148 research outputs found
Antimalarial activity of Syzygium guineense during early and established Plasmodium infection in rodent models
No full textAbstract
Background
In Ethiopia, the leaves of Syzygium guineense have been found useful for the prevention and cure of malaria, and demonstrated antiplasmodial activity in vitro. Nevertheless, no scientific study has been conducted to confirm its antimalarial activity in vivo. Therefore, the objective of the study was to evaluate the antimalarial effect of Syzygium guineense leaf extract in mice.
Methods
Inoculation of the study mice was carried out by using the malaria parasite, Plasmodium berghei . The plant extract was prepared at 200, 400 and 600\ua0mg/kg. Chloroquine and distilled water was administered to the positive and negative control groups respectively. Parameters like parasitaemia, survival time and body weight were determined following standard tests (4-day suppressive, Rane\u2019s and repository tests).
Results
Syzygium guineense crude leaf extract displayed considerable ( p \u2009<\u20090.05) parasite suppression at doses of 600 and 400\ua0mg/kg in a 4-day suppressive test with chemosuppressive value of 59.39 and 49.09% respectively. S. guineense crude leaf extract also showed dose-dependent schizontocidal activity in both the repository and curative tests. The extract also prevented body weight loss and prolonged survival date of mice significantly ( P \u2009<\u20090.05) at the highest dose employed in the study. Qualitative chemical assay for S. guineense methanolic leaf extract revealed that the plant is endowed with different plant secondary metabolites exemplified by terpenoids, alkaloids, triterpenes, flavonoids, anthraquinones, tannins, glycosides, saponins and phenols.
Conclusion
Syzygium guineense leaf extract possess antimalarial activity in mice. The test substance was found to be safe with no observable signs of toxicity in the study mice. The results of the present work confirmed the in vitro antiplasmodial finding and traditional claims in vivo in mice. Therefore, Syzygium guineense could be regarded as a potential source to develop safe, effective and affordable antimalarial agent
Regulation of protein and carbohydrate intake in caged honeybees Apis mellifera scutellata : assessment based on consumption and various performance measures
No full textDissertation (MScAgric)--University of Pretoria, 2010.When provided with the opportunity to select their diet, most insect herbivores regulate their nutrient intake. However, in a nutritionally heterogeneous environment and with changing demands for growth, development and reproduction, obtaining the required amount and balance of nutrients is a challenge. This is especially true for social insects where the workers bring food into the colony to be shared by nestmates. The ability of insects to self-select their diet is an important trait related to fitness. In this study we investigated whether and how caged worker honeybees meet their nutritional requirements in response to the nutritional composition of the food they find. Using the ‘geometric framework’ we looked at the behavioural and physiological mechanisms used by caged worker honeybees in balancing their diet when provided with different pairs of complementary imbalanced foods. First, we investigated whether caged worker honeybees maintain their intake target by providing them with pairs of complementary imbalanced foods with varying protein to carbohydrate (P:C) ratios. Diets were formulated using different protein sources: casein, royal jelly and Feed-Bee®. Honeybees self-selected or balanced their diet by switching between the complementary foods in accordance with the composition of the food and the type of protein that they encountered. Honeybees selected average P:C ratios of 1:12, 1:14 and 1:11 on casein, royal jelly and Feed-Bee® diets respectively. The level of self-selection was confirmed using two performance measures: survival and ovarian activation. Both survival and ovarian activation differed depending on the type of protein source used. Second, we investigated if honeybees regulated their growth target, which is the amount of nutrients incorporated into growth and storage tissue, by measuring physiological parameters in honeybees confined on imbalanced complementary food combinations having different P:C ratios. Feed-Bee® was used as a protein source. The physiological parameters measured were head fresh mass, hypopharyngeal gland (HPG) development, and protein concentration in the haemolymph. The bees fed on different diet combinations with different P:C ratios maintained each of the performance measures to the same level, which supports the ability of worker honeybees to self-select their diet. The measured physiological parameters were compared with other studies to asses the appropriateness Feed-Bee® diet as a protein source for the bees. In the absence of brood the intake target is directly related to the physiological requirements of the worker bees. The behavior of these individual adult bees gives an insight in to the complex system; similar responses may be seen in nurse bees in the colony condition to obtain protein, carbohydrate and other nutrient requirements from stored pollen and nectar in the hive, either for their own nutritional requirements or for other colony members, especially larvae.Zoology and Entomologyunrestricte
PLoS One
No full textBackgroundIPT with or without concomitant administration of ART is a proven intervention to prevent tuberculosis among PLHIV. However, there are few data on the routine implementation of this intervention and its effectiveness in settings with limited resources.ObjectivesTo measure the level of uptake and effectiveness of IPT in reducing tuberculosis incidence in a cohort of PLHIV enrolled into HIV care between 2007 and 2010 in five hospitals in southern Ethiopia.MethodsA retrospective cohort analysis of electronic patient database was done. The independent effects of no intervention, \u201cIPT-only,\u201d \u201cIPT-before-ART,\u201d \u201cIPT-and-ART started simultaneously,\u201d \u201cART-only,\u201d and \u201cIPT-after-ART\u201d on TB incidence were measured. Cox-proportional hazards regression was used to assess association of treatment categories with TB incidence.ResultsOf 7,097 patients, 867 were excluded because they were transferred-in; a further 823 (12%) were excluded from the study because they were either identified to have TB through screening (292 patients) or were on TB treatment (531). Among the remaining 5,407 patients observed, IPT had been initiated for 39% of eligible patients. Children, male sex, advanced disease, and those in Pre-ART were less likely to be initiated on IPT. The overall TB incidence was 2.6 per 100 person-years. As compared to those with no intervention, use of \u201cIPT-only\u201d (aHR\u200a=\u200a0.36, 95% CI\u200a=\u200a0.19\u20130.66) and \u201cART-only\u201d (aHR\u200a=\u200a0.32, 95% CI\u200a=\u200a0.24\u20130.43) were associated with significant reduction in TB incidence rate. Combining ART and IPT had a more profound effect. Starting IPT-before-ART (aHR\u200a=\u200a0.18, 95% CI\u200a=\u200a0.08\u20130.42) or simultaneously with ART (aHR\u200a=\u200a0.20, 95% CI\u200a=\u200a0.10\u20130.42) provided further reduction of TB at 3c80%.ConclusionsIPT was found to be effective in reducing TB incidence, independently and with concomitant ART, under programme conditions in resource-limited settings. The level of IPT provision and effectiveness in reducing TB was encouraging in the study setting. Scaling up and strengthening IPT service in addition to ART can have beneficial effect in reducing TB burden among PLHIV in settings with high TB/HIV burden.2014U2G PS000858/PS/NCHHSTP CDC HHS/United StatesU2GPS000858/PEPFAR/United States25105417PMC41267261023
Characterization of indigenous breeding strategies of the sheep farming communities of Ethiopia
No full textThis working paper synthesizes and analyzes the characteristics of the indigenous sheep production and breeding strategies and practices of four sheep farming communities located in pastoral (Amibara), sub-alpine sheep-barley (Menz), perennial crop-livestock (Bonga), and cereal-livestock (Horro) production systems. The paper also provides a model framework for characterizing the indigenous sheep production and breeding practices of traditional sheep producers in Ethiopia as a basis for designing suitable community-based breeding programs. Sections 1-3 of the paper present introduction to, objectives and study framework of the ICARDA-ILRI-BOKU research project.
Chapters 4 and 5 give highlights, respectively, on sheep breeding strategies and the basis for designing community-based breeding programs in Ethiopia. Section 6.1 analyses indigenous sheep production and breeding strategies and practices of sheep farming communities. The paper closes with a synthesis of approaches to the design of community-based breeding programs including definition of breeding objectives, designing, optimizing and implementing community-based breeding programs in section 6.2
Yield Effects of Plot-Level Korra Tef (Eragrostis tef) Seed Rate in Central Ethiopia: Application of the Dose-Response Model
No full textEthiopia's government and development practitioners have encouraged the utilization of improved tef (Eragrostis tef) varieties to increase crop production. However, apart from introducing improved tef varieties, more needs to be known about the plot-level yield effects of adopting these varieties. Based on this, the current study sought to investigate the yield effects of plot-level tef seed rate in Central Ethiopia, focusing on the Korra tef variety. Two Hundred Twenty One (221) Korra tef producers were selected using multi-stage stratified sampling technique. Survey data were gathered from these respondents using a questionnaire, and interview guides were used to gather qualitative data from the key informants. The yield outcomes of seed rate users categorized as users below, within, and above the recommendations were examined using one-way ANOVA. The F-test results indicated disparities in yield across the three types of seed rate users. The impacts of seed rate on yield were examined using the Dose-Response Model, which was applied to five seeding rates (12, 14, 16, 18, and 20kgha-1). According to a Dose-Response analysis, the highest average tef yield was attributed to a seed rate of 20 kgha-1, slightly higher than recommended. The findings revealed a significant correlation between the utilization of the recommended Korra seed rate and increased crop productivity among growers. It is evident that encouraging farmers to solely use improved seed varieties is insufficient. Rather, the focus should be on promoting the utilization of the recommended seed rates. Furthermore, a reassessment of the ideal Korra tef seed rate for the study area is warranted, considering the observed benefits in productivity as farmers approached the utilization of the recommended rate
Early initiation of breastfeeding and exclusive breastfeeding practices and associated factors among first-time mothers attending governmental maternal and child health clinics in Gondar town, Northwest Ethiopia: a mixed method study
No full textAbstract Background Early initiation of breastfeeding and exclusive breastfeeding are among those optimal breastfeeding practices endorsed by World Health Organization to reduce child morbidity and mortality. However, worldwide, less than half of the mothers practiced early initiation and exclusive breastfeeding including first-time mothers who need even more emphasis as their decision to initiate early and exclusively breastfeed their first child raises the likelihood of doing the same for the subsequent. This study aimed to assess early initiation and exclusive breastfeeding practices and associated factors among first-time mothers attending governmental maternal and child health clinics in Gondar town, Northwest Ethiopia. Methods A facility-based cross-sectional quantitative study on 885 first-time mothers selected by two-stage sampling complemented by a phenomenological qualitative study on 23 purposively chosen participants was conducted from February 24 to May 27, 2021. The quantitative study involved a pre-tested structured questionnaire and binary logistic regression was done for data analysis. The qualitative study involved in-depth interview, and the data was thematically analyzed. Result In this study, 74.7% (95% CI 71.8, 77.5) of the mothers practiced early initiation while 46.8% (95% CI 43.5, 50.1) of them breastfed exclusively. Vaginal delivery (AOR 5.63, 95% CI 3.64, 8.71), not giving prelacteal feeding (AOR 5.54, 95% CI 3.50, 8.78) and colostrum feeding (AOR 2.89, 95%CI 1.85, 4.52) were predictors of early initiation of breastfeeding. On the other hand; delivery at a health facility (AOR 3.13, CI 1.58, 6.18), number of PNC visits [1 visit (AOR 1.88, CI 1.27, 2.77), 2–3 (AOR 1.97, CI 1.25, 3.12) and ≥ 4 (AOR 3.61, CI 1.53, 8.54)], not giving prelacteal (AOR 2.14, CI 1.32, 3.48), husband’s support (AOR 2.34, CI 1.13, 4.83) and health workers’ support (AOR 4.34, CI 1.98, 9.53) were found to be determinants for exclusive breastfeeding which were also shown in the qualitative. Conclusion The magnitudes of early initiation and exclusive breastfeeding practices were lower than the national target and global recommendation for universal coverage plus most of the significant factors were maternal and child health service related factors. So, strengthening the services is crucial and the main focus should be on first-time mothers
Acetylation and Evaluation of Taro Boloso-I Starch as Directly Compressible Excipient in Tablet Formulation
No full textTaro Boloso-I (TB1), a newly improved Colocasia esculenta variety, is a potential source of starch with high yield. However, to improve some limitations of the native starches (NS), such as flowability and compactibility, different physical and chemical starch modifications have been employed. Acetylation is one of the chemical modifications which improves the flow and compaction of the NS, which are prerequisite during direct compression (DC) of tablets. Hence, in this study, TB1 starch was acetylated using acetic anhydride and evaluated as an ideal excipient for direct compression. Starch acetates (SA) with a degree of substitution (DS) of 0.072 (SA1) and 0.695 (SA2) were produced and evaluated. FTIR spectra of the SAs were used to verify the acetylation of the NS. Powder flow evaluation parameters showed significant improvement in the flow properties of the NS following acetylation. In addition, the swelling power, solubility, and compactibility were also improved. Tensile strength (TS) of the tablets comprising SAs only, SA1 (41.40) and SA2 (63.43 Kg/cm2), was significantly higher than tablets made of the NS (31.96) and Starch 1500® (15.12 Kg/cm2). The SAs also showed lower sensitivity towards lubrication than the NS and Starch 1500® as lower lubricant sensitivity ratios were recorded. In addition, tablets comprising the SAs satisfactorily accommodated at least up to 50 % w/w paracetamol—compared to 30 % w/w by Starch 1500®—upon DC processing. The paracetamol tablets comprising SAs also complied with the United States Pharmacopeia specifications for disintegration and dissolution studies. Therefore, taking all the facts into consideration, the SAs could be potential DC excipients in tablet formulations
The impact of Korra tef (Eragrostis tef) adoption on commercialization status of tef producing farmers in Northwestern Ethiopia: A propensity score matching analysis
No full textAbstractThe government and other development practitioners in Ethiopia have promoted crop technologies like improved tef varieties to improve crop productivity of farmers and, in turn, their commercialization status. The commercialization impacts of these crops, however, were not thoroughly examined. This study examined the commercialization impact of adopting improved tef variety (Korra) in North-western Ethiopia using cross-sectional data of 479 tef producer farm households drawn from two districts, one from the adopters and the other from non-adopters. A multi-stage sampling procedure was followed to select the respondents. A semi-structured questionnaire was used as a principal primary data collection method to collect household survey data, and interviews were conducted with the relevant key informants of the study. The extent of smallholder commercialization was examined using the Household Commercialization Index (HCI), and the impact of Korra tef adoption on adopters’ commercialization was estimated using Propensity Score Matching (PSM). The HCI result revealed that 46.95% of sampled farmers sold tef during 2020; while it was 58.92% and 36.7% for the adopters and non-adopters, respectively. This indicated that the non-adopters and adopters were, respectively, semi- and commercialized. The PSM result also revealed a positive and significant impact on households’ tef commercialization, with the adopters’ commercialization rate exceeding the non-adopters by about 23.43%. Hence, efforts should focus on ensuring that farmers have access to sufficient quantities of high-quality improved Korra tef seed as well as encouraging improved access to institutional services for the same. [Figure: see text
Data from: Carbon sequestration and soil restoration potential of grazing lands under exclosure management in a semi-arid environment of northern Ethiopia
No full textExclosures are used to regenerate native vegetation as a way to reduce soil erosion, increase rain water infiltration and provide fodder and woody biomass in degraded grazing lands. Therefore, this study assessed the impact of grazing exclosure on vegetation biomass, carbon sequestration and soil nutrients under five and ten years of grazing exclosures and freely grazed areas in Tigray, northern Ethiopia. Vegetation biomass, carbon stocks and soil nutrients increased with increasing grazing exclusion. However, open grazing lands and five years of grazing exclosure did not differ in aboveground biomass, above-and-belowground carbon stocks. Moreover, ten years of grazing exclosure had a higher (P<0.01) grass, herb and litter carbon stocks compared to five years exclosure and open grazing lands. The total carbon stock was higher for ten years exclosure (75.65 t C ha-1) than the five years exclosure (55.06 t C ha-1) and in open grazing areas (51.98 t C ha-1). Grazing lands closed for ten years had a higher SOC, organic matter, total N, available P, and exchangeable K+ and Na+ compared to five year’s exclosure and open grazing lands. Therefore, establishment of grazing exclosures had a positive effect in restoring degraded grazing lands, thus improving vegetation biomass, carbon sequestration potentials and soil nutrients
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