111 research outputs found
A Look at the Condition of Women in Malentendues by Azza Filali
No full textA Look at the Female Condition in Malentendues by Azza Filali
This article analyzes the female condition through the central character of Malentendus by Azza Filali. It explores the tensions between social injunctions and individual aspirations, highlighting how the author constructs a female figure striving for emancipation in a Tunisian context marked by deep contradictions. The study examines the narrative and discursive strategies that reveal the obstacles, resistances, and ambiguities shaping the character’s journey. Adopting a literary and socio-critical approach, this article investigates the representation of power dynamics and identity issues in the novelCe travail propose une analyse de la condition féminine à travers le personnage central de Malentendues d’Azza Filali. Il s’agit d’explorer les tensions entre injonctions sociales et aspirations individuelles, mettant en avant la manière dont la romancière présente une figure féminine en quête d’émancipation dans un contexte tunisien marqué par des contradictions majeures. L’article examine les stratégies narratives et discursives qui révèlent les obstacles, les résistances et les ambiguïtés du parcours de ce personnage. En adoptant une approche sociocritique, ce travail interroge la représentation des rapports de pouvoir et des dynamiques identitaires à l’œuvre dans le roman
Human models for White Sutton syndrome: POGZ mutations change the transcriptome and induce defects in neural progenitor cell biology
No full textIntellectual disability (ID) and autism spectrum disorders (ASD) are complex neurodevelopmental conditions with high genetic heterogeneity. Studies indicate that 10%-40% of individuals with ID also have ASD, suggesting shared molecular mechanisms between these disorders. Recent next-generation sequencing studies have highlighted a significant role of de novo mutations in ASD, particularly those with large effects. Among these, POGZ (Pogo Transposable Element with zinc finger “ZNF” domain) has emerged as a frequently mutated gene with potential loss-of-function effects in ASD patients. However, the underlying molecular mechanisms and the pathogenic impact of POGZ mutations are not fully understood.
POGZ encodes a protein that is mainly binds to heterochromatin protein 1α and contributes to gene regulatory functions. Functionally, POGZ is critical for kinetochore assembly, sister chromatid cohesion, and mitotic chromosome segregation. POGZ deficiency can lead to premature mitotic exit, polyploidy, and potential cell death or genomic instability, which may disrupt neural development and brain function. POGZ thought to act as a transcriptional regulator, potentially influencing molecular networks that are critical for neuronal function.
This PhD study investigated the cellular and molecular mechanisms by which POGZ mutations contribute to neurodevelopmental disorders (NDDs) using human induced pluripotent stem cells (iPSCs) derived from patient and CRISPR/Cas9-mediated gene editing to introduce heterozygous POGZ mutations. These mutant iPSCs were differentiated into neural progenitor cells (NPCs) and neurons under both two-dimensional (2D) and three-dimensional (3D) culture conditions to analyze the effects of POGZ mutations on neural development.
Key findings of the study indicated that frameshift mutations in the N-terminus or the HP1-binding zinc finger-like (HPZ) domain of POGZ led to decreased POGZ protein expression without disrupting its nuclear localization. Using 3D neurospheres and brain organoids, it was found that POGZ-deficient cells exhibited impaired self-renewal of NPCs, alongside enhanced differentiation and increased neuronal migration. Additionally, analysis of the transcriptome via RNA sequencing revealed widespread changes in gene expression in NPCs carrying POGZ mutations. These alterations were significantly enriched for genes involved in mitotic chromatid segregation, DNA repair, nonsense-mediated decay, and alternative splicing. Notably, the data revealed a transcriptomic signature characterized by the elevated expression of neuron-specific genes, suggesting an "accelerated differentiation" phenotype in mutant NPCs, mirroring the behavior observed in the 3D neurosphere models.
Furthermore, CUT&Tag sequencing was employed to identify direct targets of POGZ, providing evidence that POGZ directly regulated genes linked to synaptic function, chromosome segregation, and Wnt signaling. The overrepresentation of autism-associated risk genes among POGZ-regulated targets further suggested a potential link between POGZ dysfunction and the etiology of NDDs, including ASD.
This analysis emphasized the critical role of POGZ in regulating neural development at both the cellular and molecular levels. Understanding how POGZ mutations drive alterations in NPC behavior and gene regulation, is crucial for developing targeted therapeutic strategies for conditions associated with POGZ dysfunction. Ultimately, this study aimed to bridge the gap between genetic findings and pathophysiological mechanisms in NDDs, providing deeper insights into the developmental disruptions caused by POGZ mutations.197 Seiten ; Illustrationen, Diagramm
Roles and responsibilities of family home caregivers for elderly people in Qatar: Experiences and challenges
No full textThe relationship between overjet size and dentoalveolar compensation
No full textBackground : The purpose of this study was to investigate the effect of overjet size and the dento-alveolar compensation in subjects with normal class I molar relationship. Materials and Methods: Lateral cephalometric head records of 59 Egyptian children (34 boys and 25 girls) aged 7.5 to 10.5 years with mean age of 8.69±0.73. All had normal class I type of occlusion. The sample was classified into four quartiles according to the overjet size and the cephalometric analysis was based on seven linear and eight angular measurements using a dental tracer programme. Results: showed that, in spite of presence of high significant over jet size differences between the groups; there was no significant differences in all the studied parameters were found. Applying the least significant differences (LSD) test and coefficient correlations between the studied parameters clarifying that there was a significant differences in angular measurements (SN-AB, SN-Occl, I-I, I-ML, I-NB). Conclusion: during transitional dentition there was a sufficient dento-alveolar adaptation to growth changes in the saggittal jaw relation ship to attain normal class I type of occlusion. This compensation is pronounced in angular parameters and clustered in the lower arches particularly in incisal area
A Comparative Study of the Self-Assembly of Achiral and Chiral Hairy Nanoparticles with Polystyrene Cores and Poly (2- hydroxyethylmethacrylate) Hairs
No full textHairy nanoparticles with polystyrene cores (PS cores) and poly(2 hydroxyethylmethacrylate) (PHEMA) shells were synthesized by combining living anionic polymerization and atom transfer radical polymerization (ATRP). The structural characterization was carried out by FT-IR and NMR spectroscopy (1H NMR, 13C NMR, APT 13C NMR and 1H 13C HMQC). The thermal stability of the PS cores was not a?ected by grafting PHEMA on their surfaces. A di?erential scanning calorimetry (DSC) thermogram of the HNPs showed two distinct transition temperatures indicating microphase separation. Chiral HNPs were prepared by inducing chirality in the achiral HNPs by complexation with R- or S-mandelic acid. The circular dichroism (CD) spectroscopy of complexes of the HNPs/R- or S-mandelic acid indicated the formation of enantiomeric chiral structures. The self-assembled structures formed from the achiral HNPs show di?erent surface morphologies, porous and zigzag, dependent on the solvents used. Blends of polystyrene functionalized with hydroxyl groups and PHEMA show di?erent morphology and thermal properties compared with the core shell HNP system. The chiral HNPs self-assembled into donut like structures or toroids with sizes in the range between 200 to 5000 nm. The study suggests that chirality can be utilized to develop interesting self-assembled structures
Analysis of residential energy consumption characteristics: A comparative study between two cities in China and the U.S
No full textThe author has granted permission for their work to be available to the general public.This study compares and analyzes residential energy use patterns in the two dominant housing typologies in two cities with similar climates in the U.S. and China (Houston, TX and Xiamen, China). The study included developing four residential models representing a single-family-house and a multi-family-house in both Houston and Xiamen using the building performance simulation tool, eQuest. Inputs for the models were based on existing building codes, census data, government surveys, validated previous studies, and a household survey conducted in Xiamen, China. The models were used to study the impact of building physical characteristics and occupant behavior on residential energy consumption patterns in both cities. The results of the simulation were then analyzed and the outcomes of the analysis were used to identify opportunities for reducing Chinese residential energy use. The research outcomes indicate that the low energy efficiency of building envelopes and systems in Chinese houses are a major issue in Chinese residential buildings. Avoiding the potential increase in energy use in the Chinese residential sector requires taking steps to improve the efficiency of building envelopes, systems, and appliances. The research provides specific recommendations for Chinese energy conservation policy-makers as well as developers and companies working in the Chinese residential sector. The research also provides the foundation for future studies aiming to improve the energy efficiency of Chinese houses.Architectur
Kinetics of Base-catalysed Cyclization of 1,5-Dianilino-2,4-diphenylpent-1,4-diene-3-one
No full text1038-104
EFFECTS OF SENILE CHANGES AND HYDROCORTISONE ADMINISTRATION, AS COMPARED WITH CHANGES IN EXPERIMENTAL RUPTURE OF THE ACHILLt~STENDON IN RATS
No full textHartree-Fock Approach for Rotating Bose-Einstein Condensation in One-dimensional Deep Optical Lattice
No full textRange of Applying the Boundary Condition at Fluid/Porous Interface and Evaluation of Beavers and Joseph’s Slip Coefficient Using Finite Element Method
No full textIn this work, Finite Element Method (FEM) is applied to obtain the condition at the boundary of the interface between a channel and a porous medium. The boundary conditions that should be applied to the inhomogeneous interface zone between the two homogeneous regions of free fluid and porous medium are derived. The comparison has been performed for porous material characterizations to provide the velocity at the inhomogeneous interface zone with variable permeability between the two homogeneous regions of free fluid and porous medium. Also, the dependence of the slip coefficient on the thickness of the transition zone is established and the values of the thickness are so justified that the numerical results and the numerical results of our proposed technique are found to be in good agreement with experimental results in the literature
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