6 research outputs found
High potential biological stains from the traditional dyes of Manipur, India
874-879Cytogenetics depends to a large extent on the biological stains and advancement of the microscopic devices. Most important
and oldest biological stain is carmine, an animal extract. The application of natural dyes for staining of various biological tissues
from an alternative source will decrease the expense for purchasing the synthetic dye and reduce their effects on human and
environment. The plant derivative dyes were screened for biological staining in the present study. Two of the most popular
traditional vegetative dyes in Manipur are extracted from Bixa orellana (local name Ureirom, UR) and Strobilanthes cusia
(local name KUM, KU). The water extracts of two the plants were taken to study for the stainability of nuclear on root tip cells
of Allium ascalonicum L. to test the feasibility of the dyes as the biological stains. The different stages of mitosis cell division in
A. ascalonicum were stained with the dyes of KU and UR and compared with the standard stain acetocarmine. The UR stain is
nonspecific as it stains whole cytoplasm as well as the nuclear parts. The KU stained the nuclear parts more precisely than UR
and was as good as acetocarmine. The nuclear stainability of KU or UR is significant in the sense that these are natural products
with no allergic response as that of carmine and it is time tested (particularly in Manipur). Hence, KU and UR are promising
candidates for cytological/biological application in future that will be cost effective and environmental friendly. In future these
two could be used as food colourant for human consumption
SEA BUCKTHORN (HIPPOPHAE SP.): HIGHLY POTENTIAL UNDERUTILISED PLANT IN SIKKIM: A REVIEW
Seabuckthorn (SB) has gained tremendous importance as a multi utility plant. SB products include foodstuffs, healthcare products, cosmetics, medicine and many more. Countries like China, Russia, and Canada exploited the plant with immense commercial success. Seabuckthorn grow in Lachung and Lachen area of North Sikkim. The berries were mainly use as dye and meat tenderiser and leaves as fodder in Sikkim. SB holds the potential of changing the socio economic status of rural Sikkim if the plant is exploited like China and elsewhere but government must have proper policy and R&D backup to realised the potential
Distinct differentiation of closely related species of Bacillus subtilis group with industrial importance
High potential biological stains from the traditional dyes of Manipur, India
Cytogenetics depends to a large extent on the biological stains and advancement of the microscopic devices. Most important and oldest biological stain is carmine, an animal extract. The application of natural dyes for staining of various biological tissues from an alternative source will decrease the expense for purchasing the synthetic dye and reduce their effects on human and environment. The plant derivative dyes were screened for biological staining in the present study. Two of the most popular traditional vegetative dyes in Manipur are extracted from Bixa orellana (local name Ureirom, UR) and Strobilanthes cusia (local name KUM, KU). The water extracts of two the plants were taken to study for the stainability of nuclear on root tip cells of Allium ascalonicum L. to test the feasibility of the dyes as the biological stains. The different stages of mitosis cell division in A. ascalonicum were stained with the dyes of KU and UR and compared with the standard stain acetocarmine. The UR stain is nonspecific as it stains whole cytoplasm as well as the nuclear parts. The KU stained the nuclear parts more precisely than UR and was as good as acetocarmine. The nuclear stainability of KU or UR is significant in the sense that these are natural products with no allergic response as that of carmine and it is time tested (particularly in Manipur). Hence, KU and UR are promising candidates for cytological/biological application in future that will be cost effective and environmental friendly. In future these two could be used as food colourant for human consumption.
Fermented foods affect the seasonal stability of gut bacteria in an Indian rural population
Abstract The effect of fermented foods on healthy human gut microbiota structure and function, particularly its seasonal preference and frequent long-term consumption, has been largely uncharacterised. Here, we assess the gut microbiota and metabolite composition of 78 healthy Indian agrarian individuals who differ in the intake of fermented milk and soybean products by seasonal sampling during hot-humid summer, autumn and dry winter. Here we show that, seasonal shifts between the Prevotella- and Bifidobacterium/Ruminococcus-driven community types, or ecological states, and associated fatty acid derivatives, with a bimodal change in Bacteroidota community structure during summer, particularly in fermented milk consumers. Our results associate long-term fermented food consumption with reduced gut microbiota diversity and bacterial load. We identify taxonomic groups that drive the seasonal fluctuation and associated shifts between the two ecological states in gut microbiota. This understanding may pave the way towards developing strategies to sustain a healthy and resilient gut microbiota through dietary interventions
