1,720,977 research outputs found
α7-integrin enhancing compounds regulate α7β1-integrin and laminin-α2 expression in airway smooth muscle cells via miRNA-mediated gene silencing mechanisms
No full textThe University of Nevada, Reno Libraries will promptly respond to removal requests related to content that violates intellectual property laws, data protections, or has been uploaded without creator consent. Takedown notices should be directed to our ScholarWolf team ([email protected]) with information about the object, including its full URL and the nature of your complaint.Asthma is characterized by airway inflammation, mucus production, and airway smooth muscle hyperreactivity that result in difficulty breathing. There has been an increasing need to develop new treatments for asthma that target airway smooth muscle in order to decrease airway remodeling. Previous studies have shown that ?7?1-integrin, a transmembrane protein receptor that promotes extracellular laminin-?2 binding, maintains a contractile airway smooth muscle phenotype and inhibits airway smooth muscle cell proliferation. However, the molecular mechanisms by which ?7-integrin regulates airway smooth muscle phenotype and function remain unknown. The hypothesis is that compounds targeting ?7-integrin expression regulate airway smooth muscle phenotype via miRNA-mediated gene silencing mechanisms. Our laboratory has verified that miR-124a binds to the 3’untranslated region of ?7-integrin and other studies have identified compounds modulating ?7-integrin expression in skeletal muscle. Non-asthmatic and asthmatic human airway smooth muscle cells were treated with these compounds and then transcript levels of ?7?1-integrin, laminin- ?2, and miR-124a were analyzed by quantitative real-time polymerase chain reaction. Results showed an inverse relationship between miR-124a and the expression of ?7?1- integrin and laminin-?2 in treated asthmatic differentiated cells
miR-25 expression inhibits proliferation and supports a healthy contractile phenotype in a mouse model of allergic inflammation
No full textThe University of Nevada, Reno Libraries will promptly respond to removal requests related to content that violates intellectual property laws, data protections, or has been uploaded without creator consent. Takedown notices should be directed to our ScholarWolf team ([email protected]) with information about the object, including its full URL and the nature of your complaint.Asthma is a respiratory illness that can be primarily characterized by airway remodeling, airway hyperresponsiveness, and inflammation. While current treatments help to ease symptoms, they do little to treat these underlying causes of asthma. This results in poorly controlled symptoms and raises the need for novel therapeutic targets. One such potential target is miR-25, a microRNA demonstrated in human airway smooth muscle cells to inhibit key cell division pathways. This study aims to test the hypothesis that miR-25 regulates airway smooth muscle phenotypes in a mouse model of allergic inflammation. To investigate the role of miR-25 in proliferation, tissue samples were obtained from second and third generation of transgenic mice in which miR-25 overexpression was targeted to smooth muscle cells (TgSM-miR-25) and wild-type (WT) mice treated with ovalbumin or an adjuvant control. Protein samples from tissues were subjected to Western blot analysis using proliferating cell nuclear antigen or cyclin D1 antibodies. To determine the effects of miR-25 on contractile protein expression, blots from the above experiment were re-probed with smooth muscle myosin heavy chain and smooth muscle myosin heavy chain. In lung tissue from ovalbumin-treated TgSM-miR-25 mice, proliferating cell nuclear antigen expression was significantly decreased compared to the wild-type group. Additionally, smooth muscle myosin heavy chain and smooth muscle-?-actin protein levels were significantly increased in the same tissues. Together these data provide evidence that miR-25 mediates airway smooth muscle phenotype in mice by inhibiting airway smooth muscle cell division and contributed to the development of 2 a contractile phenotype. Thus, miR-25 may be a novel therapeutic target for the development of new asthma therapies
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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