213 research outputs found
supplementary_table_primers - Disruption of mitochondrial membrane potential coupled with alterations in cardiac biomarker expression as early cardiotoxic signatures in human ES cell–derived cardiac cells
No full textsupplementary_table_primers for Disruption of mitochondrial membrane potential coupled with alterations in cardiac biomarker expression as early cardiotoxic signatures in human ES cell–derived cardiac cells by Saras Jyoti and Simran Tandon in Human & Experimental Toxicology</p
Sistem Kontrol Motor Pompa Pengisi Tower Air (Tandon)
No full textThe need for clean water has become a basic need that must be met every day. The community needs a lot of water consumption but sometimes a little. Due to the irregular use of water, it will result in the supply of water in the reservoir will quickly decrease which results in the volume of water in the tendon will run out. Usually to monitor the water supply in the water storage tank (tower) using an ordinary pump, where humans fill the pump continuously or permanently. This has an impact on the reduction of water supply due to erratic usage. The author with the title "Pump Motor Control System Filling Water Tower (Tandon)" which aims if at the time of use of water expenditure is very much, the volume in the reservoir will quickly decrease. Then the pump motor will accelerate the automatic rotation so that it will quickly fill the volume of water in the reservoir. So that the tendon is not empty due to more use. And if at the time of using a small amount of water discharge, the volume in the reservoir will decrease slowly, the pump motor will automatically rotate slowly. This will be efficient and flexible due to the uncertainty of excessive usage so that the volume of water in the reservoir does not empt
Achyranthes aspera Root Extracts Induce Human Colon Cancer Cell (COLO-205) Death by Triggering the Mitochondrial Apoptosis Pathway and S Phase Cell Cycle Arrest
No full textAchyranthes aspera (AA) has been used traditionally for the cure of various disorders. However, the action of root extracts of AA as anticancer agent and its cellular mechanism remain unclear. The aim was to screen the antitumor effect of ethanolic (EAA) and aqueous (AAA) root extracts on the growth of colon cancer COLO-205 cells by testing their cytotoxicity, followed by their effect on clonogenicity, migration, and induction of apoptosis. Mechanisms leading to apoptosis and cell cycle arrest were also investigated by expression studies of caspase-9, caspase-3, Bax, Bcl-2, p16, p21, and p27 genes, followed by flow cytometric analysis for cell cycle distribution. Cytotoxicity screening of AA extracts indicated greater cytotoxic activity of AAA extract against COLO-205 cells. A series of events marked by apoptosis revealed loss of cell viability, chromatin condensation, and DNA fragmentation in AAA treated cells to a greater extent. The mRNA expression levels of caspase-9, caspase-3, Bax, p16, p21, and p27 were markedly increased in the AAA treated cells, along with decreased Bcl-2 expression. The cell cycle arrest at S phase was detected by flow cytometric analysis after treatment with AAA. Overall the study signifies the aqueous extracts as a promising therapeutic candidate against cancer
Disruption of mitochondrial membrane potential coupled with alterations in cardiac biomarker expression as early cardiotoxic signatures in human ES cell–derived cardiac cells
No full textEvaluation of the Cytotoxic Effects of CAM Therapies: An In Vitro Study in Normal Kidney Cell Lines
No full textThe purpose of this current study was to justify the incorporation of complementary and alternate medicine (CAM) in current cancer treatments. The major drawback of anticancer drugs is their nonselective killing, which ultimately leads to attrition of normal cells. Keeping this as the foundation of our study, we made an effort to compare the cytotoxicity associated with a known chemotherapeutic drug 5-Fluorouracil (5-FU), with certain CAM therapies previously reported to have anticancer activity. The parameters chosen for the study were based on antiproliferative and cytotoxic effects on normal, kidney epithelial cells (NRK-52E). The MTT assay, colony formation assay, DNA fragmentation, and differential staining using AO/EB, following treatment with either 5-FU or CAM therapies, were performed. The CAM therapies under study were various extracts of wheatgrass, roots of Achyranthes aspera (AA), mushroom extracts (Pleurotus ostreatus, Macrolepiota procera, and Auricularia polytricha), and a homeopathic drug, Ruta graveolens (Ruta). The results showed that treatment of normal cells with the CAM therapies led to minimum cell damage in comparison to 5-FU. This evidence-based study will lead to greater acceptance of alternative therapies against cancer
Role of Matrix Metalloproteinases in Degenerative Kidney Disorders
No full text
Matrix metalloproteinases (MMPs) are members of calcium dependent-zinc
containing endopeptidases that play a pivotal role in extracellular matrix (ECM) remodeling.
MMPs are also known to cleave non-matrix proteins, including cell surface receptors,
TNF-α, angiotensin-II, growth factors, (especially transforming growth factor-β1, ΤGF-
β1) plasminogen, endothelin and other bioactive molecules. The tissue inhibitors of metalloproteinases
(TIMPs) inhibit the activity of MMPs and decrease ECM degradation.
Various patho-physiological conditions have been linked with the imbalance of ECM synthesis
and degradation. Numerous studies have reported the significance of MMPs and
TIMPs in the progression of kidney pathologies, including glomerulonephritis, diabetic
nephropathy, renal cancer, and nephrolithiasis. Although dysregulated activity of MMPs
could directly or indirectly lead to pathological morbidities, their contribution in disease
progression is still understated. Specifically, MMP activity in the kidneys and it's relation
to kidney diseases has been the subject of a limited number of investigations. Therefore,
the aim of the present review is to provide an updated insight of the involvement of
MMPs and TIMPs in the pathogenesis of inflammatory and degenerative kidney disorders.
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Inclusive Design for Regaining Lost Identity: Accessible, Aesthetic and Effortless Clothing
No full textInclusive Design for Regaining Lost Identity is a research project that aims to design and develop accessible clothing that is inclusive and fashionable to foster psycho-social well-being and to enhance the self-esteem of women with disabilities. The research explored inclusive design solutions for the saree – a traditional Indian garment for women, through a practice-led iterative methodology of designing and producing pragmatic and aesthetic sarees with enhanced ease of dressing and undressing. These sarees are intended to offer the participants a sense of independence and individuality whilst responding to their needs for self-expression. Clothing for persons with disabilities frequently follows a medical model which focuses on their inabilities. Such experiences tend to aggravate feelings of inadequacy and lower self-esteem due to the limited choices of pertinent fashionable clothing. Ethnographic research was conducted with women in the age range of twenty to forty years with mild to moderate physical disabilities, including those with cerebral palsy, reduced dexterity and limb amputations. Investigations into apparel-related barriers they face and their expectations and desires about clothing pointed to a huge void in terms of designed inclusive clothing for women with physical disabilities. Self-efficacy emerged as a significant criteria for the selection of garments. A series of informed study prototypes of adaptive sarees were designed and developed along with an exploration into the feasibility of 3D printed fasteners intended to alleviate potential feelings of frustration and deprivation while wearing saree is an elaborate garment that requires precise draping, which makes the saree complicated to wear and inconvenient to carry, particularly for women with physical disabilities. For many women in India, the saree remains the customary dress, especially for work and occasions, yet minimal progress and advancement has been made to enhance its accessibility and ease of use. This research aspires to provide inclusive design solutions that seamlessly embrace persons with disabilities, to elevate their agency and to challenge and change prevailing negative attitudes, misconceptions, and stereotypes in relation to their specific needs and desires
Corporate governance and equity prices : evidence from the Czech and Slovak Republics
No full textThe 1992 Czechoslavakia mass privatization program involving about 1,500 eneterprises and implemented through a voucher scheme with competitive bidding was a bold step in changing the ownership and governance of a large part of the economy. It represents a clear test case of one approach, and other countries may benefit from its lessons. At the time, much skepticisism was voiced about mass privatization: it would lead to diffuse ownership, and no effective corporate governance would result. But innovative forces led to the emergence of investment funds that collected much of the individuals'voucher points, leading to a much more concentrated ownership structure. It has been expected that this concentrated ownership would lead to improved corporate governance. But the jury is still out. So far, only limited and largely anecdotal evidence is available on the impact investment funds have on the way firms are being managed. Too little time has passed and too many shocks have occurred (for example, the split of the Czech and Slovak Republics) to expect to find discernible changes in corporate governance on measures of actual firm performance. An alternative approach is to investigate whether firms that ended up with more concentratedownership -- and possibly improved governance -- sell for higher prices, either in the last voucher round or in the secondary market since then. In a forward-looking financial market, one can expect prices to incorporate the effects of better ownership on future firm performance and associated dividends to shareholders. Put differently, one would expect that two firms with different shareholding structures, but otherwise identical, would trade at different prices -- with the firm with a more concentrated ownership, and presumably better corporate governance, trading at a higher price. On a cross-sectional basis, ownership structure may thus be significant in explaining (relative) share prices. The author explores this line of reasoning. Controlling for a number of firm and sector-specific variables: he finds: 1) Majority ownership by a domestic or foreign investor has a positive influence on firm prices. 2) Firms with many small owners have lower prices. 3) Ownership by many small scale investors makes it easier for any single investor to establish effective control, but such control does not necessarily translate into higher prices. The author also provides two possible explanations of why higher prices appear to be associated only with majority ownership by a single investor: he finds: 1) The corporate legal framework and the difficulty in collecting proxy votes in the Czech and Slovak Republics may prevent a small investor from making the necessary changes in the way firms are managed, thus keeping prices low; and 2) Commercial banks are both managers of invesment funds and creditors of individual firms. Funds managers may face conflicts of interest and not be interested in increasing the value of equity alone but also the value of credits. This could explain why prices are relatively lower for those firms in which investment funds have effective control.Economic Theory&Research,Payment Systems&Infrastructure,International Terrorism&Counterterrorism,Environmental Economics&Policies,Labor Policies,International Terrorism&Counterterrorism,Payment Systems&Infrastructure,Environmental Economics&Policies,Financial Intermediation,Economic Theory&Research
Reduction of oxalate-induced renal tubular epithelial (NRK-52E) cell injury and inhibition of calcium oxalate crystallisation in vitro by aqueous extract of Achyranthes aspera
No full textMechanistic Insights into the Antilithiatic Proteins from Terminalia arjuna: A Proteomic Approach in Urolithiasis.
No full textKidney stone formation during hyperoxaluric condition is inherently dependent on the interaction between renal epithelial cells and calcium oxalate (CaOx) crystals. Although modern medicine has progressed in terms of removal of these stones, recurrence and persistent side effects restricts their use. Strategies involving plant based agents which could be used as adjunct therapy is an area which needs to be explored. Plant proteins having antilithiatic activity is a hitherto unexplored area and therefore, we conducted a detailed identification and characterization of antilithiatic proteins from Terminalia arjuna (T. arjuna). Proteins were isolated from the dried bark of T. arjuna and those having molecular weights > 3 kDa were subjected to anion exchange chromatography followed by gel filtration chromatography. Four proteins were identified exhibiting inhibitory activity against CaOx crystallization and crystal growth kinetics The cytoprotective and anti-apoptotic efficacy of these purified proteins was further investigated on oxalate injured renal epithelial cells (MDCK and NRK-52E) wherein, injury due to oxalate was significantly attenuated and led to a dose dependent increase in viability of these cells. These proteins also prevented the interaction of the CaOx crystals to the cell surface and reduced the number of apoptotic cells. Identification of these 4 anionic proteins from the bark of T. arjuna was carried out by Matrix-assisted laser desorption/ionization-time of flight Mass spectrometry (MALDI-TOF MS). This was followed by database search with the MASCOT server and sequence similarity was found with Nuclear pore anchor, DEAD Box ATP-dependent RNA helicase 45, Lon protease homolog 1 and Heat shock protein 90-3. These novel proteins isolated from T. arjuna have the potential to inhibit CaOx crystallization and promote cell survival and therefore, offer novel avenues which need to be explored further for the medical management of urolithiasis
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