22 research outputs found
Isolation of new genes involved in temperature synchronization of the circadian clock of Drosophila melanogaster
No full textPhDCircadian clocks regulate behaviour and physiology of many organisms and keep
them in synchrony with the environment. Drosophila's circadian clock is mainly
synchronized by natural light-dark cycles and temperature
fluctuations, both at
molecular and behavioural levels. The mechanisms underlying temperature entrainment
are poorly understood, but previous studies have shown that this process
can be genetically dissected.
In this work, I isolated several mutants which interfere with the temperature
synchronization of Drosophila's circadian clock. Three variants were isolated
in a chemical EMS-mutagenesis screen monitoring putative second- and thirdchromosomal
mutations. The mutants behave normal in light-dark cycles suggesting
that they specifically interfere with temperature entrainment. In a different,
RNAi-based screen, a Forkhead-domain transcription factor encoding gene was
isolated, which shows defective circadian activity of per expression and PER accumulation
in temperature-entrainment condition, when down-regulated. Finally,
a candidate approach led me to identify three genes encoding proteins belonging
to the TRP family of ion channels. Mutations in the pyrexia, trpM and trpA1
genes show abnormal temperature synchronization of locomotor behaviour, similar
to our EMS-candidates.
The isolation and analysis of those mutations are described, as well as a behavioural
analysis of the already-known "temperature-mutant" nocte. In particular,
I discuss the involvement of chordotonal organs as structures required for
temperature entrainment of the clock and the role of nocte for signalling the temperature
information from the periphery to the brain.
The rest-activity pattern is a well-studied circadian output behaviour; the pupal
emergence, named eclosion, is another behaviour strictly regulated by the circadian clock. Here we show that genes important for entrainment of adult locomotor
behaviour to temperature do not play the same role in regulating the synchronization
of eclosion. To gain insight into the synchronization mechanisms of eclosion,
I studied how different entrainment conditions affect the phase and free-running
period of eclosion
A VIEW OF THE WHOLE THAT ECHOES THE TOTAL THEORY
No full textAbstract: The structure of the Whole is briefly described and is what TOTALITARIAN THEORY brings. There is a one-dimensional, two-dimensional and three-dimensional world of real dimensions. Behind them there are the imaginary space, but there are also the dimensions of time, real and imaginary. Simple motion determines the time Dt, which is the same in all three dimensions of a movement.
While space has three dimensions with the corresponding imaginary ones, time, as defined, has the three dimensions with the corresponding imaginary ones belonging to the goddess and so many others that belong to the ultimate reality. It is the world of 18 dimensions.
Finally, it remains to create the world of 24 dimensions, which will have in addition to the dimensions of the time of the goddess and which will have a distant zero and another six that will belong to the man who will become a creator
Keywords: Totalitarian Theory, real dimensions.
Title: A VIEW OF THE WHOLE THAT ECHOES THE TOTAL THEORY
Author: ALEKOS CHARALAMBOPOULOS
International Journal of Mathematics and Physical Sciences Research
ISSN 2348-5736 (Online)
Vol. 10, Issue 1, April 2022 - September 2022
Page No: 59-67
Research Publish Journals
Website: www.researchpublish.com
Published Date: 12-September-2022
DOI: https://doi.org/10.5281/zenodo.7072430
Paper Download Link (Source)
https://www.researchpublish.com/papers/a-view-of-the-whole-that-echoes-the-total-theoryInternational Journal of Mathematics and Physical Sciences Research, ISSN 2348-5736 (Online), Research Publish Journals, Website: www.researchpublish.co
THE NEW PHYSICS
No full textAbstract: With the correct mathematics, it turns out that scientifically Ohm's law is not correct and the capacity and resistance of the coil proportional to the square of the charge and the change of current respectively. A new theory of electricity is created.
By overturning the explanation given by Edison to the emission of electrons from the incandescent lamp, we are changing physics, since we no longer accept electrons and protons. In their place there are two bubbles of sparse ether, proposed by my cosmic theory THE IODION. They are electrical bodies opposite (electric charge opposite), they roam circularly and create electric current I = ef. Charges and currents are attracted and comes the new theory of the atom, partially different at low pressure of the element gas with it in one atmosphere. And the unit system is differentiated.
It is proved by unshakeable mathematics, that when one body is attracted to another and rotates circularly around the other, then the force, the attraction, is inverse of the square of their radius. This overturns the established theory of the solar system! And the earth is light if we correct the Cavedish's mistake, and in its core it has a plasma surrounded by dense gases
Keywords: changing physics, incandescent lamp, electrical bodies, solar system!.
Title: THE NEW PHYSICS
Author: ALEKOS CHARALAMPOPOULOS
International Journal of Mathematics and Physical Sciences Research
ISSN 2348-5736 (Online)
Vol. 10, Issue 1, April 2022 - September 2022
Page No: 19-25
Research Publish Journals
Website: www.researchpublish.com
Published Date: 24-May-2022
DOI: https://doi.org/10.5281/zenodo.6576528
Paper Download Link (Source):
https://www.researchpublish.com/papers/the-new-physicsInternational Journal of Mathematics and Physical Sciences Research, ISSN 2348-5736 (Online), Research Publish Journals, Website: www.researchpublish.co
THE FINAL FORM OF ATOMIC THEORY
No full text<p><strong>Abstract:</strong> We will find the centripetal force that we will apply to atomic physics, which is very different from the accepted physics. We will find the speeds and accelerations hidden in a circular smooth motion. We formulated some of why atomic physics was misformulated, and we proved the area and volume of spheres, different from the existing ones.</p>
<p>We set principles, like the ancient Greek philosophers, to formulate the theory.</p>
<p>We formulated the two different magnetic fields, which produce a circularly rotating charge.</p>
<p>We have formulated the existence of the ether. An ether with low viscosity does not contradict the Mickelson-Morley experiment and much more, it does not contradict the geocentric system of the universe, where the earth is almost stationary.</p>
<p><strong>Keywords:</strong> Atomic Theory, atomic physics, geocentric system.</p>
<p><strong>Title:</strong> THE FINAL FORM OF ATOMIC THEORY</p>
<p><strong>Author:</strong> ALEKOS CHARALAMPOPOULOS</p>
<p><strong>International Journal of Mathematics and Physical Sciences Research </strong></p>
<p><strong>ISSN 2348-5736 (Online)</strong></p>
<p><strong>Vol. 11, Issue 2, October 2023 - March 2024</strong></p>
<p><strong>Page No: 1-9</strong></p>
<p><strong>Research Publish Journals</strong></p>
<p><strong>Website: www.researchpublish.com</strong></p>
<p><strong>Published Date: 02-October-2023</strong></p>
<p><strong>DOI: <a href="https://doi.org/10.5281/zenodo.8398908">https://doi.org/10.5281/zenodo.8398908</a></strong></p>
<p><strong>Paper Download Link (Source)</strong></p>
<p><strong><a href="https://www.researchpublish.com/papers/the-final-form-of-atomic-theory">https://www.researchpublish.com/papers/the-final-form-of-atomic-theory</a></strong></p>International Journal of Mathematics and Physical Sciences Research, ISSN 2348-5736 (Online), Research Publish Journals, Website: www.researchpublish.co
Genetic control strategies for population suppression in the Anopheles gambiae complex: a review of current technologies
No full textMalaria continues to pose a critical public health threat, with mosquitoes from the Anopheles gambiae complex acting as the main vectors of the disease in sub-Saharan Africa, where approximately 95% of malaria-related deaths occur. Despite significant advancements in vector control, such as insecticide-treated bed nets and indoor spraying, the effectiveness of these interventions is increasingly compromised by various challenges, including rising levels of insecticide and pathogen resistance, mosquito behavioural adaptations, and persistent funding gaps. In this context, genetic vector control strategies have shown considerable promise, primarily based on findings from controlled laboratory studies. This review explores the development of these genetic approaches within the Anopheles gambiae complex and outlines future directions for their advancement and potential integration into malaria control efforts
A body broken: a critical biography of Alekos Doukas (1900-1962)
No full textThis thesis is a critical biography as cultural history that tracks the life of an Asia Minor Greek, Alekos Doukas (1900-1962), through his childhood years, exile, wartime experience, refugee life in the 1920s and migration to Australia where he is gradually radicalised in the 1930s. In the 1940s and 1950s he becomes an activist and published author. Through a close study of a literary archive of letters and manuscripts, Doukas is located and analysed as an historical subject within the broader events and discourses of the first half of the twentieth century. The thesis works to open up the neglected field of migrant subjectivity in which cultural history has been flattened by assimilationist monocultural theoretical frameworks. The thesis critically examines contemporary Greek-Australian historiographical and literary accounts that construct Doukas in terms of a mythologised figure and that read his 1950s socialist realist novels as unproblematic autobiography and historical testimony. The study explores the cultural history of a minority group through a transnational perspective within broad Australian cultural debates about national history, ongoing colonialist discourses, ethnicity and race, memorialisation, and the competing claims of fiction and history. The thesis constructs a biographical narrative that focuses on the interplay between broad discursive systems and everyday practice, addressing in the process questions of agency, location and cultural specificity. It analyses Doukas within the broad cultural and political crisis that followed WWI and the Greek-Turkish war, as well as the transition of the Greek diaspora from traditional trading to industrial migrant patterns of overseas settlement. The methodology is informed by critical discourse analysis and, in particular, Edward Said's Orientalism which provides a model for the movement from broad analysis to close textual readings. The study analyses original sources in Greek and Greek-Australian cultural history, connecting a wide range of theoretical and historical perspectives. The thesis is also informed by contemporary research that seeks to rewrite the separate national narratives of communities in the old Ottoman Empire as interconnected and entangled histories. Finally, the thesis also focuses on the corporeal aspects of memory and the materiality of culture in which intellectual and cultural phenomena operate through the location of a unique body
A male-biased sex-distorter gene drive for the human malaria vector Anopheles gambiae
No full textOnly female insects transmit diseases such as malaria, dengue and Zika; therefore, control methods that bias the sex ratio of insect offspring have long been sought. Genetic elements such as sex-chromosome drives can distort sex ratios to produce unisex populations that eventually collapse, but the underlying molecular mechanisms are unknown. We report a male-biased sex-distorter gene drive (SDGD) in the human malaria vector Anopheles gambiae. We induced super-Mendelian inheritance of the X-chromosome-shredding I-PpoI nuclease by coupling this to a CRISPR-based gene drive inserted into a conserved sequence of the doublesex (dsx) gene. In modeling of invasion dynamics, SDGD was predicted to have a quicker impact on female mosquito populations than previously developed gene drives targeting female fertility. The SDGD at the dsx locus led to a male-only population from a 2.5% starting allelic frequency in 10-14 generations, with population collapse and no selection for resistance. Our results support the use of SDGD for malaria vector control
Cryptochrome Antagonizes Synchronization of Drosophila’s Circadian Clock to Temperature Cycles
No full textSummaryBackgroundIn nature, both daily light:dark cycles and temperature fluctuations are used by organisms to synchronize their endogenous time with the daily cycles of light and temperature. Proper synchronization is important for the overall fitness and wellbeing of animals and humans, and although we know a lot about light synchronization, this is not the case for temperature inputs to the circadian clock. In Drosophila, light and temperature cues can act as synchronization signals (Zeitgeber), but it is not known how they are integrated.ResultsWe investigated whether different groups of the Drosophila clock neurons that regulate behavioral rhythmicity contribute to temperature synchronization at different absolute temperatures. Using spatially restricted expression of the clock gene period, we show that dorsally located clock neurons mainly mediate synchronization to higher (20°C:29°C) and ventral clock neurons to lower (16°C:25°C) temperature cycles. Molecularly, the blue-light photoreceptor CRYPTOCHROME (CRY) dampens temperature-induced PERIOD (PER)-LUCIFERASE oscillations in dorsal clock neurons. Consistent with this finding, we show that in the absence of CRY very limited expression of PER in a few dorsal clock neurons is able to mediate behavioral temperature synchronization to high and low temperature cycles independent of light.ConclusionsWe show that different subsets of clock neurons operate at high and low temperatures to mediate clock synchronization to temperature cycles, suggesting that temperature entrainment is not restricted to measuring the amplitude of such cycles. CRY dampens temperature input to the clock and thereby contributes to the integration of different Zeitgebers
Dōmu di Katsuhiro Otomo. Dal reale all’immaginario, l’architettura come parte integrante della narrazione
No full textKatsuhiro Otomo (Hasama, 1954) è universalmente riconosciuto come uno dei più importanti maestri dell’industria del fumetto giapponese. Akira (1982), storia di fantascienza che presenta una location caratterizzata dai sterminati grattacieli, è sicuramente la sua opera più nota a livello internazionale. Due anni prima di dar vita al mondo di Akira, Otomo gettò le basi della creazione di uno scenario urbano coerente in Dōmu, Sogni di Bambini. La storia di Dōmu è interamente ambientata in un condominio intensivo giapponese, un enorme serpentone chiamato Tsutsumi Danchi. Un’architettura che Otomo rappresenta in modo fin troppo detta-gliato per essere una sua invenzione. Il riferimento per lo Tsutsumi infatti è lo Shibazono Danchi, un edificio di abitazioni intensive situato nella prefettura di Saitama. Otomo, come un regista navigato, seleziona la location, distorcendola e plasmandola rispetto alla sua visione. Attraverso prospettive drammatiche e dettagliatissime, Otomo rende l’edificio a tutti gli effetti uno dei protagonisti del racconto. Il paper si propone di indagare quanto il disegno riesca a manipolare una semplice architettura suburbana per soddi-sfare una visione creativa. Un oggetto morto, inanimato, all’interno della narrazione diventa vivo, pulsante di informazioni. Il disegno permette così, al pari di una inquadratura cinematografica di un autore visionario, di creare un nuovo mondo (tangenziale a quello reale di riferimento) ma assolutamente nuovo e che vive di vita propria.Katsuhiro Otomo (Hasama, 1954) is universally recognized as one of the masters of the Japanese comics industry. Akira (1982), a science fiction story set in a location characterized by endless skyscrapers, is certainly his most internationally known work. Two years before giving life to the world of Akira, Otomo laid the foundations for the creation of a coherent urban scenario in Dōmu, A Child’s Dream. Dōmu’s story is entirely set in an intensive Japanese condominium, a huge snake building called Tsutsumi Danchi. An architecture that Otomo represents in too much detail to be his own invention. The reference for Tsutsumi is in fact the Shibazono Danchi, a building of intensive housing located in the Saitama prefecture. Otomo, like an expert director, selects the location, distort-ing and shaping it to fit his vision. Through dramatic and highly detailed perspectives, Otomo makes the Tsutsumi Danchi one of the protagonists of the story. The paper aims to investigate how much drawing allows one to manipulate a simple suburban architecture to satisfy a creative vision. A dead inanimate object within the narrative becomes alive, pulsating with information. The drawing thus allows, like a cinematographic shot of a visionary author, to create a new world (tangential to the real one) but absolutely new and which has a life of its own
