1,721,514 research outputs found
Analysis of HIF-1 alpha and HIF-2 alpha in dendritic cells stimulated under hypoxic conditions
Full text linkAnalysis of HIF-1 alpha and HIF-2 alpha in dendritic cells stimulated under hypoxic conditions Barroeta Seijas AB.1, Filippi I.2, Vitale S. 1, Simonetti S. 3, Naldini A.2, Di Rosa F.1 1 Institute of Biology and Molecular Pathology (IBPM)- National Research Council (CNR), Rome, Italy 2 Department of Physiology, University of Siena, Siena, Italy. 3 Department of Molecular Medicine, University of Rome “Sapienza”, Rome, Italy Dendritic cells (DCs) are professional antigen-presenting cells that have a pivotal role in linking innate and acquired immunity, and in the maintenance of selftolerance. DCs can activate resting T cells in spleen and lymph nodes, stimulate effector T cells in inflamed tissues and contribute to maintenance of memory T cells in different organs, including the bone marrow (BM). Alternatively activated DCs have been involved in regulation of angiogenesis during chronic inflammation. Inflamed tissues and BM share a common feature: a low oxygen tension. Under hypoxic conditions, different levels of hypoxia inducible factors HIF-1 alpha and HIF-2 alpha modulate the expression of a variety of genes related to apoptosis, cell migration, metabolic changes necessaries for adaptation to the hypoxic environment. Although it is generally believed that hypoxic conditions are inhibitory for protective immune responses, a clear picture of the hypoxia-induced pathways in DCs has not emerged yet. We aim to investigate the molecular and functional changes induced by hypoxia in mouse BM-derived DCs. Specifically, we will evaluate the regulation of the transcription factors HIF-1 alpha and HIF-2 alpha in DCs stimulated with classical (e.g. LPS) and alternative stimuli (e.g. IL-10), under either normoxic or hypoxic conditions. We will investigate the production by stimulated DCs of molecular mediators affecting both angiogenesis and T cell responses. We expect to gain more information on the cross-talk between angiogenic molecules and cytokines involved in immune regulation, occurring both in hypoxic lymphoid organs and inflamed tissues
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Inquinamento ambientale e Mycobacterium chelonae: a proposito di un caso di ipodermite nodulare suppurativa in immunodepressa
No full textVariations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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