1,720,983 research outputs found
Facial transplantation. An update of results and perspectives from tissue engineering
No full textFacial transplantation is a revolutionary procedure developed recently, which is indicated if autologous transfers fail to restore human appearance. More than 30 patients have undergone facial transplantation in different centers worldwide. Here, we provide an update on its main anatomical, surgical, immunological, ethical, and follow- up aspects. We also provide innovative perspectives of regenerative medicine and tissue engineering that could hold promise for this emerging surgical field
Facial transplantation. An update of results and perspectives from tissue engineering
No full textFacial transplantation is a revolutionary procedure developed recently, which is indicated if autologous transfers fail to restore human appearance. More than 30 patients have undergone facial transplantation in different centers worldwide. Here, we provide an update on its main anatomical, surgical, immunological, ethical, and follow- up aspects. We also provide innovative perspectives of regenerative medicine and tissue engineering that could hold promise for this emerging surgical field
Stem cells derived from adipose tissue: Possible uses and clinical applications
No full textIn the past adipose tissue was considered only as the energy storage of our body, today we can talk about adipose organ, an active and dynamic tissue from which multipotent stem cells can be obtained. The aim of our study was to focus on the properties and possible uses of stem cells derived from adipose tissue (ASCs) in regenerative medicine based on the major scientific studies reported in international literature. Referring to current scientific literature, we have described the main properties of ASCs by focus on the sources from which they can obtained, isolation procedures, clinical applications and the current legislation on their use. ASCs are multipotent stem cells with paracrine, immunomodulatory, chemotactic and differentiating activity that promote their use in various fields of regenerative medicine and tissue engineering. Currently there is no universally recognized protocol regarding the isolation procedure. Under current European legislation, ASCs can only be used if autologous, non-cultivated, mechanically isolated and transferred at the level of a homologous tissue. To date, clinical protocols that do not comply with these guidelines are considered off-lable. Today, there is a growing need for a protocol shared by the international scientific community on the stem cells isolation procedure from adipose tissue and on their clinical applications in compliance with current legislation
La liposuzione quale fonte di cellule staminali
No full textLIPECTO MY AS A SOURCE OF STEM CELLS Over the last few years, interest in adipose tissue as the ideal source of mesenchymal stem cells (MSCs) has considerably grown. MSCs represent the future of regenerative medicine, both for their multipotent capabilities and for their ability to excretion of pro-regenerative cytokines. Mesenchymal stem cells derived from adipose tissue (ASC) have become important in regenerative medicine due their abundance in adipose tissue and ease of liposuction extraction. To obtain adipose tissue, several harvesting techniques exist. Liposuction is a safe and reliable aesthetic surgical technique to obtaining ASC in large quantities, but few studies have highlighted the effects of different liposuction techniques on ASC regeneration capacity and their ASC functionality. Several studies have been considered that have assessed whether the various liposuction approaches could have influenced the vitality of ASCs. We also reported our ASC withdrawal and isolation techniques, which allowed us to isolate a quantity of 9.06 ×105 ASC from 100 mL of adipose tissue, with ASC yield of 25.9% of the pellet. The use of mesenchymal stem cells (MSC) in clinical research and applications has continued in recent decades. By comparing the various liposuction techniques in literature, we concluded that standard aspiration-assisted liposuction remains the most effective and least traumatic ASC extraction method
From liposuction to adipose-derived stem cells: indications and technique
No full textAdipose tissue is an organ of energy storage, an endocrine organ, a soft tissue filler and a cosmetically unnecessary tissue discarded by liposuction. Liposuction was designed to correct unaesthetic deposits of subcutaneous fat; it produces satisfactory silhouette contouring when performed by appropriately trained operators using properly selected technologies. However, from lipoaspirate it is possible to obtain autologous fat graft and adipose-derived stem cells (ASCs) for reconstructive surgery and regenerative medicine. Autologous fat transplantation uses include the correction of body contour, malformations and post-surgical outcomes. The regenerative properties of ASCs allow treating damaged tissues such as wounds, burns, scars and radiodermatitis. The aim of this study was to perform a literature review highlighting the crucial role of adipose tissue in plastic and reconstructive surgery, from liposuction to lipofilling and ASCs, exposing the indications, procedures and complications of these surgical techniques
Autologous fat transplantation for secondary breast reconstruction: Our experience
No full textBackground. Autologous fat transplantation is used after breast reconstruction to improve the breast profile. There are a variety of different methods used for fat harvesting, preparation, and reinjection. This study describes the specific techniques we used in this series of autologous fat transplantations in breast reconstruction patients and reports their outcomes compared with other studies in the literature. Patients and methods. At the University Hospital of Parma between May 2012 and December 2016, we performed 53 autologous fat transplantations for secondary breast reconstruction patients with an average age of 49 years (range: 34â65 y). A tumescent fluid (NaCl, epinephrine, and a local anaesthetic) was injected, and the lipoaspirate was harvested using a closed aspirationâinjection system connected to a 50 ml syringe, a 4 mm infiltration cannula, and a -650 mmHg vacuum. The average amount of lipoaspirate obtained was 100 ml (range: 50â200 ml). Centrifugation of the lipoaspirate (3000 rpm for 3 min) was performed to isolate the adipose tissue (average amount obtained, 80 ml; range: 30â180 ml). Under local anaesthesia, the retrograde injection of thin layers of fat graft in multiple tunnels was performed in the subcutaneous and/or subglandular planes. Results. Average follow-up was six months. Comparable to other studies, our complication rate was 7.4% (n = 4/53) and included cyst formation at the injection site (n = 1/53) and hematoma at the donor site (n = 3/53). Repeat fat grafting was performed in 28.3% of patients (n = 15/53) due to fat graft resorption. Conclusions. Autologous fat transplantation is a useful procedure for correcting irregularities in the breast contour in secondary breast reconstruction
Adipose-derived stem cells: Comparison between two methods of isolation for clinical applications
No full textOff-label use of adipose-derived stem cells
Full text linkBackground Adipose-derived stem cells (ASCs) have a broad range of clinical applications. The ease of cell harvest and high yield with minimal donor-site morbidity makes adipose tissue an ideal source of stem cells. Further, the multi-lineage potential of these cells present significant opportunities within the field of tissue engineering, with studies successfully demonstrating their ability to produce a range of tissue types. Materials and methods Literature review of publications on the use of ASCs, in the context of current European and US regulations. Results According to European and US regulations, many clinical trials reported in literature to date could be considered off-label. Conclusion In Europe, clinical trials involving cultured ASCs and/or the use of collagenase, which causes changes in the structural and functional properties of stem cells, and/or ASCs application in non-homologous tissue, should be considered off-label. ASCs should be non-cultured, isolated mechanically, and used only in the subcutaneous tissue
The art and science beyond body contouring a solution for massive weight loss patients
No full textThe massive weight loss often resulted in an excess of loose skin mainly in the abdomen, upper arms, thighs, chest, back, and laterally on the back. Consequently, most patients sought for a solution, provided by the plastic surgery through the body-contouring surgery. Abdominoplasty, arm lift, tight lift, breast lift, and liposuction are all body contouring procedures that aim to reshape the patients' body. Furthermore, body-contouring surgery can improve the patients' physical discomfort in daily life, also helping in the maintenance of normal BMI (body mass index) in formerly obese patients, thus having a positive aesthetic and psychosocial outcome. A key component of body contouring after massive weight loss is managing patient expectations. Understanding the risks of body contouring's procedures is important for patient counseling and the informed consent process. The goal of this study was to perform a literature review concerning the indication, surgical technique, outcomes and complications of body-contouring surgery
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