1,721,033 research outputs found
Potential Applications of DPSCs in Regenerative Medicine: Differentiation Ability and Support in the Angiogenesis Process
No full textProtocol for the generation and analysis of organoids from Dental pulp stem cells (DPSCs)
No full textDPSCs are a valuable resource for creating three-dimensional (3D) in vitro models, such as organoids, due to their accessibility and differentiation potential. Organoids have revolutionized in vitro culture, thanks to their ability to more faithfully replicate the architecture and complexity of tissues compared to 2D cultures. This enables their use in a wide range of applications, including research on genetic diseases, innovative therapies, and tissue engineering. This project aims to establish an in vitro protocol for generating 3D models from DPSCs to study dental diseases and test new drugs for regenerative medicine and tissue reconstruction. To this end, the organoids underwent morphological, viability, and functional analyses, allowing us to evaluate the effectiveness of the protocol and the validity of the model. The protocol includes the culture of DPSCs in Matrigel (R) Matrix, an extracellular matrix that supports 3D-fabricated organoids formation and growth. These 3D structures are exposed to differentiation factors to generate dental pulp organoids. However, the presence of Matrigel (R) Matrix may interfere with subsequent molecular analyses. To overcome this limitation, an efficient method for removing the Matrigel (R) Matrix was developed to allow efficient extraction of nucleic acids and proteins. Immunohistochemical and immunofluorescence techniques were also optimized to visualize cellular structures and markers. Cell count and proliferation were assessed using a CCK-8 viability assay, as well as Trypan Blue staining. The feasibility and effectiveness of this protocol can provide a new tool for studying dental pulp biology, paving the way for future applications in the field of dental pulp regeneration research
Mesenchymal Stem Cells and Their Role in Neurodegenerative Diseases
No full textMesenchymal stem cells (MSCs) have garnered significant interest in the field of regenerative medicine for their ability to potentially treat various diseases, especially neurodegenerative disorders [...
Study of CCM Microvascular Endothelial Phenotype by an In Vitro Tubule Differentiation Model
No full text
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Study of CCM Microvascular Endothelial Phenotype by an In Vitro Tubule Differentiation Model
No full textVariations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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