5,360 research outputs found
Effectiveness, Against Tuberculosis, Of Pseudo-ternary Complexes: Peptide-dna-cationic Liposome
No full textWe report the effects of a synthetic peptide designed to act as a nuclear localization signal on the treatment of tuberculosis. The peptide contains 21 amino acid residues with the following specific domains: nuclear localization signal from SV 40T, cationic shuttle sequence, and cysteamide group at the C-terminus. The peptide was complexed with the plasmid DNAhsp65 and incorporated into cationic liposomes, forming a pseudo-ternary complex. The same cationic liposomes, composed of egg chicken l-α-phosphatidylcholine, 1,2-dioleoyl-3-trimethylammonium-propane, and 1,2-dioleoyl-3-trimethylammonium-propane (2:1:1. M), were previously evaluated as a gene carrier for tuberculosis immunization protocols with DNAhsp65. The pseudo-ternary complex presented a controlled size (250. nm), spherical-like shape, and various lamellae in liposomes as evaluated by transmission electron microscopy. An assay of fluorescence probe accessibility confirmed insertion of the peptide/DNA into the liposome structure. Peptide addition conferred no cytotoxicity in vitro, and similar therapeutic effects against tuberculosis were seen with four times less DNA compared with naked DNA treatment. Taken together, the results indicate that the pseudo-ternary complex is a promising gene vaccine for tuberculosis treatment. This work contributes to the development of multifunctional nanostructures in the search for strategies for in vivo DNA delivery. © 2011 Elsevier Inc..3731102109Gomes, D.C.D., Pinto, E.F., de Melo, L.D.B., Lima, W.P., Larraga, V., Lopes, U.G., Rossi-Bergmann, B., (2007) Vaccine, 25, p. 2168Bao, L., Gao, L., Bao, Y., (2010) Int. J. Infect. Dis., 14, pp. E446Han, D.J., Weiner, D.B., Sin, J.I., (2010) Biomol. 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Gene Med., 11, p. 66Perrie, Y., Frederik, P.M., Gregoriadis, G., (2001) Vaccine, 19, p. 3301Rosada, R.S., de la Torre, L.G., Frantz, F.G., Trombone, A.P.F., Zarate-Blades, C.R., Fonseca, D.M., Souza, P.R.M., Coelho-Castelo, A.A.M., (2008) BMC Immunol., 9, p. 13de la Torre, L.G., Rosada, R.S., Trombone, A.P.F., Frantz, F.G., Coelho-Castelo, A.A.M., Silva, C.L., Santana, M.H.A., (2009) Colloids Surf., B, 73, p. 175Nakaie, C.R., Oliveira, E., Vicente, E.F., Jubilut, G.N., Souza, S.E., Marchetto, R., Cilli, E.M., (2011) Bioorg. Chem., 39, p. 101Kalderon, D., Roberts, B.L., Richardson, W.D., Smith, A.E., (1984) Cell, 39, p. 499Subramanian, A., Ranganathan, P., Diamond, S.L., (1999) Nat. Biotechnol., 17, p. 873McKenzie, D.L., Kwok, K.Y., Rice, K.G., (2000) J. Biol. Chem., 275, p. 9970Simeoni, F., Morris, M.C., Heitz, F., Divita, G., (2003) Nucleic Acids Res., 31, p. 2717Wetzer, B., Byk, G., Frederic, M., Airiau, M., Blanche, F., Pitard, B., Scherman, D., (2001) Biochem. 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Vaccine Immunol., 13, p. 876Rosada, R.S., de la Torre, L.G., Frantz, F.G., Trombone, A.P., Zarate-Blades, C.R., Fonseca, D.M., Souza, P.R., Coelho-Castelo, A.A., (2008) BMC Immunol., 9, p. 38de la Torre, L.G., Rosada, R.S., Trombone, A.P., Frantz, F.G., Coelho-Castelo, A.A., Silva, C.L., Santana, M.H., (2009) Colloids Surf., B, 73, p. 175Lange, A., Mills, R.E., Lange, C.J., Stewart, M., Devine, S.E., Corbett, A.H., (2007) J. Biol. Chem., 282, p. 5101Mandavilli, A., (2007) Nat. Med., 13, p. 268Lasic, D.D., (1997) Nature, 387, p. 26Tokunaga, M., Hazemoto, N., Yotsuyanagi, T., (2004) Int. J. Pharm., 269, p. 71Subramanian, A., Ranganathan, P., Diamond, S.L., (1999) Nat. Biotechnol., 17, p. 87
Domain Names in Turkey
No full textTurkey faces both unique and common domain name regulatory issues. In this paper, the author focuses on the challenges ahead for Turkey, with especial focus on the regulation of IDNs
Plagiarism: concepts and contexts
No full textAfter outlining the key difficulties in modern literature on academic plaigarism, the author suggests that these may be resolved by a new model of plaigarism (whilst noting that this new model does raise additional uncertainties, e.g. as to the status of 'self plagiarism') that she has developed
Schmidarius kondratieffi Santos-Silva, Heffern, Botero & Nascimento 2020
No full textSchmidarius kondratieffi Santos-Silva, Heffern, Botero & Nascimento, 2020 Figures 35–36 Schmidarius kondratieffi Santos-Silva et al., 2020: 6; Monné, 2022b: 743 (cat.). Schmidarius kondratieffi was described based on a single female from Mexico (Mexico). We have examined two additional females, which allows us to establish new state records and point out some morphological variations. These specimens differ from the holotype (see photographs on Bezark 2022) by the antennomere III slightly slender, sinuous, and less densely setose (Figs. 35–36), and the mesoventral process has no distinct extension on each side near the apex (not projected on the mesocoxae) (Fig. 36). As the other morphological features perfectly agree with those of the holotype, we believe that they are intraspecific variations. Material examined. MEXICO, Jalisco (new state record): Nevado de Colima Road, 7 mi. W hwy. junct. (near Atenquique), 1 female, 5.VIII.1978, Plitt & Schaffner leg. (FSCA). Michoacán (new state record): Parque Nacional Cerro de Carnica, Mi Ranchito, km 179.5, on Hwy 15, 8300′, 1 female, 24.VIII.2002, C.L. Bellamy leg. (MZSP).Published as part of Nearns, Eugenio H., Swift, Ian P. & Santos-Silva, Antonio, 2023, Three new species of longhorned beetles (Coleoptera: Cerambycidae) with notes on additional taxa, pp. 137-156 in Zootaxa 5228 (2) on page 153, DOI: 10.11646/zootaxa.5228.2.3, http://zenodo.org/record/753224
Distribution Of Environmental Compartments Of Herbicides Used In The Cotton, Coffee And Citrus Cultures [distribuição Nos Compartimentos Ambientais Dos Herbicidas Utiilizados Nas Culturas De Algodão, Café E Citros]
No full textThe aim of this paper was to evaluate the environmental fate of herbicides (acetochlor, 2,4-D, diuron, clomazone, thidiazuron, paraquat, simazine, fluazifo-p-butil, clethodim, oxufluorfen, flumioxazin, carfentrazone-ethyl, ametrina, trifluralin and MSMA) in cotton, coffee and citrus cultivation areas, applying the level I fugacity model. The methodology basically used the chemical and physical characteristics of the pesticides, environmental compartments and the fugacity equations. The preliminary evaluation of risk of contamination due to the use of these pesticides on the cultures studied was carried out swiftly, based on the chemical and physical properties of these products as the level I fugacity model was applied. For most of the herbicides evaluated, the water compartment was the most vulnerable. The prediction evaluation study using fugacity level I was found to be a relevant tool for the environmental fate of the herbicides studied for cotton, coffee and citrus cultures.271123132Bru, R., Carrasco, J.M., Paraíba, L.C., Unsteady state fugacity model by a dynamic control system (1998) Appl. Math Model., 22 (7), pp. 485-494Calamari, D., Vighi, M., Bacci, E., The use of terrestrial plant biomass as a parameter in the fugacity model (1987) Chemosphere, 16 (10-12), pp. 2359-2364Mackay, D., Paterson, S., Calculating fugacity (1981) Environ. Sci. Technol., 15 (9), pp. 1006-1014Mackay, D., (1991) Multimedia Environmental Models: The Fugacity Approach, p. 257. , Boca Raton: Lewis PublishersParaíba, L.C., (1999) Modelización de la Fugacidad Mediante Sistemas Dinámicos Discretos, p. 199. , Tesis (Doctoral) - Universidad Politécnica de Valência, Valência, 1999Paraíba, L.C., Carrasco, J.M., Bru, R., Level IV fugacity model by continuous time control system (1999) Chemosphere, 38 (8), pp. 1763-1775Paraíba, L.C., Simulation of the fate of the insecticide carbifuran in a rice field using a level IV fugacity model (2007) Span J Agric Res., 5 (1), pp. 43-50Plese, L.P.M., Silva, C.L., Foloni, L.L., Previsão da distribuição ambiental dos herbicidas utilizados na cultura da cana-de-açúcar (2006), p. 133. , CONGRESSO BRASILEIRO DA CIÊNCIA DAS PLANTAS DANINHAS, 25., 2006. Brasília. Anais... BrasíliaPlese, L.P.M., Silva, C.L., Foloni, L.L., Previsão da distribuição ambiental dos herbicidas utilizados na cultura do milho (2006), p. 134. , CONGRESSO BRASILEIRO DA CIÊNCIA DAS PLANTAS DANINHAS, 25., 2006, Brasília. Anais... BrasíliaSilva, C.L., Previsão ambiental da distribuição dos pesticidas aplicados na cultura do arroz (2007) Pestic.: R. Ecotoxicol Meio Amb., 17 (1), pp. 75-86Tomlin, C.D.S., (2000) The Pesticide Manual, , Farnham: British Crop Protection CouncilYenigün, O., Sohtorik, D., Calculation with the level II fugacity model for selected organophosphorus insecticides (1995) Water Air Soil Poll., 84 (1), pp. 175-18
Determination Of Anions, Cations, And Sugars In Coconut Water By Capillary Electrophoresis
No full textThis work presents the determination of several analytes in coconut water by using capillary electrophoresis with contactless conductivity detection (CCD). It was analyzed 5 different samples: two unprocessed (natural) coconut water and three processed (commercial) ones. The samples were injected without pretreatment and only a simple dilution was necessary. The technique was applied to quantify 12 analytes: chloride, sulfate, phosphate, malate, ascorbate, potassium, sodium, calcium, magnesium, fructose, glucose, and sucrose with limit of detection (LOD) of 0.09, 0.12, 0.50, 0.97, 1.2, 0.05, 0.17, 0.12, 0.08, 5.4, 7.4, and 8.9 mg L-1, respectively. These analysis were done utilizing four different running buffers. ©2005 Sociedade Brasileira de Química.166 A11341139Banzon, J.A., (1977) Philippine J. Coconut Studies, 2, p. 13Seow, C.C., Gwee, C.N., (1997) Int. J. Food Sci. Tecnol., 32, p. 189Aragão, W.M., A Importância Do Coqueiro-Anão Verde, , http://www.embrapa.brSantoso, U., Kubo, K., Ota, T., Tadokoro, T., Mackawa, A., (1996) Food Chem., 7, p. 299Aleixo, P.C., Nóbrega, J.A., Santos, D., Muller, P.C.S., (2000) Quim. Nova, 23, p. 310Dey, G., Sachan, A., Ghosh, S., Mitra, A., (2003) Ind. Crops Prod., 18, p. 171Leong, L.P., Shui, G., (2002) Food Chem., 76, p. 69Wagner, K.-H., Derkits, S., Herr, M., Schuh, H., Elmadfa, I., (2002) Food Chem., 78, p. 375Andrade, E.R., Amorim, C.A., Matos, M.H.T., Rodrigues, A.P.R., Silva, J.R.V., Dode, M.A.N., Figueiredo, J.R., (2002) Small Ruminant Res., 43, p. 235Jackson, J.C., Gordon, A., Wizzard, G., McCook, K., Rolle, R., (2004) J. Sci. Food Agric., 84, p. 1049Petroianu, G.A., Kosanovic, M., Shehatta, I.S., Mahgoub, B., Saleh, A., Maleck, W.H., (2004) J. Trace Element. Exp. Med., 17, p. 273Khan, M.N., Muti-Ur-Rehman, Khan, K.W., (2003) Pakistan J. Botany, 35, p. 925Brito, N.M., Navickiene, S., Polese, L., Jardim, E.F.G., Abakerli, R.B., Ribeiro, M.L., (2002) J. Chromatogr. A, 957, p. 201Ge, L.Y., Yong, J.W.H., Tan, S.N., Yang, X.H., Ong, E.S., (2004) J. Chromatogr. A, 1048, p. 119Da Silva, J.A.F., Guzman, N., Do Lago, C.L., (2002) J. Chromatogr. A, 942, p. 249Da Silva, J.A.F., Do Lago, C.L., (1998) Anal. Chem., 70, p. 4339Rocha, F.R., Da Silva, J.A.F., Do Lago, C.L., Fornaro, A., Gutz, I.G.R., (2003) Atmos. Environ., 37, p. 105Da Silva, J.A.F., Ricelli, N.L., Carvalho, A.Z., Do Lago, C.L., (2003) J. Braz. Chem. Soc., 14, p. 265Munoz, R.A.A., Richter, E.M., De Jesus, D.P., Do Lago, C.L., Angnes, L., (2004) J. Braz. Chem. Soc., 15, p. 523Carvalho, A.Z., Da Silva, J.A.F., Do Lago, C.L., (2003) Electrophoresis, 24, p. 213
Dictionary of Acoustics
No full textThe science and technology of acoustics embraces an unusually wide range of disciplines, from aircraft noise reduction to ultrasonics in medicine, from psychoacoustics to signal processing. The student of acoustics has to become familiar with a corresponding range of specialist terms in order to communicate with others and to understand the literature. Here, in one informative dictionary, for the first time, are listed accurate and helpful definitions to provide the student - or the specialist from another discipline - with a point of entry into the world of acoustics. The dictionary's 2,800 entries cover most of the essential concepts and terminology that the practicing acoustician needs to understand, outside the subfields of music and speech communication. The author has drawn on experience gained during a long career spent mostly at Southampton University's multidisciplinary Institute of Sound and Vibration Research, supplemented by the expertise and perspective of a team of subject specialists
Also By The Same Author: AKTiveAuthor, a Citation Graph Approach to Name Disambiguation
No full textThe desire for definitive data and the semantic web drive for inference over heterogeneous data sources requires co-reference resolution to be performed on those data. In particular, name disambiguation is required to allow accurate publication lists, citation counts and impact measures to be determined. This paper describes a graph-based approach to author disambiguation on large-scale citation networks. Using self-citation, co-authorship and document source analyses, AKTiveAuthor clusters papers, achieving precision of 0.997 and recall of 0.818 over a test group of eight surname clusters
Modelling of turbulent jets and wall layers: extensions of Lighthill's acoustic analogy with application to computational aeroacoustics
No full textTwo extensions to Lighthill’s aeroacoustic analogy are presented. First, equivalent sources due to initial conditions are derived that supplement those due to boundary conditions, as given by Ffowcs Williams & Hawkings. The resulting exact inhomogeneous wave equation is then reformulated with pressure rather than density as the wave variable, and the right-hand side is rearranged using the energy equation with no additional assumptions. Applications to computational aeroacoustics are discussed, and illustrated with examples based on 2D and 3D simulations
Comparison Of Potassium And Sodium Content In Diet And Non-diet Soft Drinks By Using Capillary Electrophoresis With Capacitively Coupled Contactless Conductivity Detection
No full textCapillary electrophoresis (CE) with capacitively coupled contactless conductivity detection (C4D) was used for determination of sodium and potassium concentrations in diet and non-diet soft drinks. Higher sodium concentrations were found in the diet samples due to the utilization of sodium salts of cyclamate and saccharine as sweeteners. The CE-C 4D method can be used by food industries and health regulatory agencies for monitoring sodium and potassium content, not only in soft drink but in many others food products.3435156Macgregor, G.A., (2001) Am. J. Kidney Dis., 37, pp. S34He, F.J., MacGregor, G.A., (2001) Br. Med. J., 323, p. 497Toseto, A., (2005), Master's Dissertation, Universidade Estadual de Ponta Grossa, BrazilTavares, M.F.M., (1996) Quim. Nova, 19, p. 173Jorgenson, J.W., Lukacs, K.D., (1981) Anal. Chem., 53, p. 1298Da Silva, J.A.F., Lago, C.L., (1998) Anal. Chem., 70, p. 4339Silva, J.A.F.D., Guzman, N., Lago, C.L., (2002) J. Chromatogr. a, 942, p. 249Munhõz, R.A.A., Richter, E.M., Jesus, D.P., Lago, C.L., Angnes, L., (2004) J. Braz. Chem. Soc., 15, p. 523Richter, E.M., Munhõz, R.A.A., Jesus, D.P., Lago, C.L., Angnes, L., (2005) J. Braz. Chem. Soc., 16, p. 1134Carvalho, A.Z., Da Silva, J.A.F., Lago, C.L., (2003) Electrophoresis, 24, p. 2138Gong, X.Y., Hauser, P.C., (2006) Electrophoresis, 27, p. 468Samcova, E., Tuma, P., (2006) Electrophoresis, 18, p. 15
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