1,720,976 research outputs found

    Two Antarctic endophytic bacteria of Colobanthus quitensis show functional and genomic characteristics potentially responsible for plant growth promotion and cold tolerance

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    Bacterial endophytes reside in plant tissues and can promote plant growth under abiotic stresses. Complex microbial communities are associated with cold-adapted plants, but scarce information is available on the functional properties of Antarctic bacterial endophytes. This study aimed to investigate possible cold tolerance and plant growth promotion activities of two Antarctic bacterial endophytes by in vitro functional characterization and genome sequence analysis. Ewingella sp., Pseudomonas sp, and their bacterial consortium were cold tolerant and showed plant growth-promoting activity on tomato seedlings at low temperature. Phytohormones (indole-3-acetic acid) and proteases were produced by Ewingella sp. and Pseudomonas sp., respectively, while ammonia and siderophores were produced by both bacterial isolates and their consortium. Ewingella sp. and Pseudomonas sp. genomes encompassed genes possibly involved in plant growth promotion (e.g., auxin, cytokinin, ethylene, salicylic acid, and siderophore metabolism and transport) and genes related to bacterial metabolic processes that can contribute to plant growth-promoting activities, such as amino acid metabolism, iron transport, nitrogen metabolism, and lytic activities (amylases, cellulases, and proteases), phosphate metabolism, potassium transport, and zinc transport. Moreover, Ewingella sp. and Pseudomonas sp. encompassed genes possibly associated with bacterial cold tolerance that can contribute to cold stress mitigation in the plant host, such as cold shock- and heat shock-related proteins, lipid desaturases, polyamine metabolism, proline metabolism, proline and glycine betaine transport, reactive oxygen species detoxification, and trehalose metabolism. Antarctic bacterial endophytes include multiple characteristics to survive under cold conditions and some bacterial functions can contribute to plant growth promotion and stress mitigation at low temperatur

    Insight into the mechanism of conidial germination of the powdery mildew mycoparasite Ampelomyces quisqualis

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    Conidia of Ampelomyces quisqualis are able to infect powdery mildew but they must be supplied with exogenous nutrients before they are able to germinate. In the present study, conidia of three genetically different strains of A. quisqualis were stimulated by different carbon and nitrogen sources and results showed nutritional supplements at various concentrations enhanced the hyphal growth and conidial germination of A. quisqualis. These stimulators induced early swelling of conidia and enhanced early germ tube branching leading to increase the mycoparasitic activity of the fungu

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Grapevine protection: from proof of concepts to pre-industrial biofungicides

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    New low-impact molecules or BCAs are continually developed to answer at the high request of more eco-friendly biofungicides. Only few of them are able to substitute preexisting pesticide and become registered bio-fungicides with a formulation study that promise a good efficacy over time. Fondazione Edmund Mach, and in particular our group, optimised the “modus operandi” in order to validate a potential biofungicide with lab proof results on leaf disks and greenhouse and field trials on grapevine plants. A lot of products, coming from internal or external projects or important pharmaceutical companies, were tested from the lab to the field, through the greenhouse until the pre-registration stage and some became a commercial product. The data collected are necessary to complete the Dossier of the product

    Variations on the Author

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    “Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship

    Appropriate Similarity Measures for Author Cocitation Analysis

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    We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis

    Fungal biodiversity of apple bark, leaves, stems, and fruit under rain shelters with reduced fungicide schedule

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    The use of rain shelters is a promising agronomic practice to protect crops from rainfall, reducing the need for fungicides to control certain pathogens that take advantage of leaf wetness. However, the combined condition of absence of rain and reduced fungicide schedule can affect the fungal populations, possibly favoring biocontrol agents and/or other pathogens. In this study, the effects this practice on epiphytic and endophytic fungal communities associated with barks, leaves, flowers, and fruits of two apple cultivars (Fuji and Golden Delicious) were evaluated across two seasons. Apple plants were grown under two conditions in a commercial-like orchard: (1) covered by rain shelters with reduced fungicide schedule and (2) uncovered with standard integrated pest management (IPM) schedule. The use of rain shelters combined with reduced fungicide applications affects the overall fungal community structure and their abundance of specific taxa. Leaf epiphytes were the most impacted community, and fungal communities also differed between the two apple cultivars. The use of rain shelters helped reduce fungicide input in the orchard, but it increased the abundance of potential pathogens compared to the IPM in open field conditions, such as powdery mildew and apple scab. Understanding how the plant microbiome responds to new practices that help in reducing fungicides can help developing strategies that avoid the build-up of potentially new pathogens
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