1,721,762 research outputs found
Optimization of Gel Elution Liquid Fraction Entrapment Electrophoresis Fractionation Coupled with Reversed Phase Liquid Chromatography Separation for Deep Proteome Analysis
No full textSiyu Wu ; Biochemistry; siyu.wu‐[email protected]. Faculty mentor: Si Wu; Chemistry and Biochemistry; [email protected]
Optimization of Gel Elution Liquid Fraction Entrapment Electrophoresis Fractionation Coupled with Reversed Phase Liquid Chromatography Separation for Deep Proteome Analysis
No full textSiyu Wu ; Biochemistry; siyu.wu‐[email protected]. Faculty mentor: Si Wu; Chemistry and Biochemistry; [email protected]
Implementing a Neutral Chemical Coating to Spray - Capillary Device for Ultra Low Volume Sample Analysis
No full textDrew King ; Chemical Engineering; Drew.N.King‐[email protected]. Faculty mentor: Si Wu; Chemistry and Biochemistry; [email protected]
Implementing a Neutral Chemical Coating to Spray - Capillary Device for Ultra Low Volume Sample Analysis
No full textDrew King ; Chemical Engineering; Drew.N.King‐[email protected]. Faculty mentor: Si Wu; Chemistry and Biochemistry; [email protected]
Importance of wine-treated Angelica Sinensis Radix in Si Wu Tang, a traditional herbal formula for treating womens ailments
No full textSi Wu Tang (Four Agents Decoction), a traditional Chinese decoction composed of Angelica Sinensis Radix, Chuanxiong Rhizoma, Paeoniae Radix Alba, and Rehmanniae Radix Praeparata in a ratio of 1: 1: 1: 1, has been used to treat womens diseases for more than a thousand years. According to the original description of Si Wu Tang, Angelica Sinensis Radix should be treated with wine. However, the importance of this wine-treated Angelica Sinensis Radix in Si Wu Tangs function has not been identified. In this article, the chemical and biological properties of two decoctions processed in different ways (Si Wu Tang with crude Angelica Sinensis Radix and Si Wu Tang with wine-treated Angelica Sinensis Radix) were compared for examination. The herbal decoction Si Wu Tang prepared from wine-treated Angelica Sinensis Radix contained much different amounts of its active compounds. Compared with Si Wu Tang using crude Angelica Sinensis Radix, Si Wu Tang prepared from wine-treated Angelica Sinensis Radix had better biological responses. Therefore, these findings accentuate the functional importance of herbs treated with wine in the Chinese decoction.</p
UPLC-QTOF-MS with chemical profiling approach for rapidly evaluating chemical consistency between traditional and dispensing granule decoctions of Tao-Hong-Si-Wu decoction
No full textAbstract Background In the present study, chemical consistency between traditional and dispensing granule decoctions of Tao-Hong-Si-Wu decoction was rapidly evaluated by UPLC-QTOF-MS coupled with the MarkerLynx software. Two different kinds of decoctions, namely traditional decoction: water extract of mixed six constituent herbs of Tao-Hong-Si-Wu decoction, and dispensing granules decoction: mixed water extract of each individual herbs of Tao-Hong-Si-Wu decoction, were prepared. Results Chemical difference was found between traditional and dispensing granule decoctions, and albiflorin, paeoniflorin, gallic acid, amygdalin, and hydroxysafflor yellow A were identified as the significantly changed components during decocting Tao-Hong-Si-Wu decoction. All the peaks of mass spectrum from Tao-Hong-Si-Wu decoction and each herb were extracted and integration by using QuanLynx™. And the optimized data was used for linear regression analysis. The contribution of each herb in Tao-Hong-Si-Wu decoction, and the optimal compatibility proportion of dispensing granule decoction were derived from the linear regression equation. Conclusions The optimal dosage proportionality of Tao-Hong-Si-Wu dispensing granule decoction was obtained as 2.5:0.2:1:0.5:0.6:0.1 (DG : CX : BS : SD : TR : HH), which guided better clinic application of Tao-Hong-Si-Wu decoction as dispensing granule decoctions usage, and it also provided some experimental data to reveal the compatibility rule of the relative TCM formulae.</p
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Chinese Herbal Formulas Si-Wu-Tang and Er-Miao-San Synergistically Ameliorated Hyperuricemia and Renal Impairment in Rats Induced by Adenine and Potassium Oxonate
Full text linkBackground/Aims: Hyperuricemia is an independent risk factor for chronic kidney disease and cardiovascular disease. Here, we examined the combined protective effects of Chinese herbal formula Si-Wu-Tang and Er-Miao-San on hyperuricemia and renal impairment in rats. Methods: Rats were randomly divided into normal rats, hyperuricemic rats, and hyperuricemic rats orally administrated with benzbromarone (4.5 mg·kg-1·d-1), Si-Wu-Tang (3.78 g·kg-1·d-1) and Si-Wu-Tang plus Er-Miao-San (6.48 g·kg-1·d-1) for 4 weeks. Hyperuricemic rats were orally gavaged with adenine (0.1 g·kg-1·d-1) and potassium oxonate (1.5 g·kg-1·d-1) daily for 4 weeks. Serum uric acid, creatinine, total cholesterol (TCH), triglyceride and blood urea nitrogen (BUN) concentrations, as well as urinary uric acid and microalbuminuria were measured weekly. Serum xanthine oxidase (XOD) activity and renal histopathology were also evaluated. The renal expression of organic anion transporter 1 (OAT1) and organic anion transporter 3 (OAT3) was detected by western blot. Results: Si-Wu-Tang plus Er-Miao-San lowered serum uric acid, creatinine, triglyceride and BUN levels to a greater degree than did Si-Wu-Tang alone. Si-Wu-Tang plus Er-Miao-San ameliorated microalbuminuria and renal histopathology, as well as decreased serum TCH concentration and XOD activity in hyperuricemic rats. Combination of Si-Wu-Tang and Er-Miao-San also led to a greater increase in OAT1 and OAT3 expression than did Siwutang alone. Conclusion: Si-Wu-Tang and Er-Miao-San synergistically ameliorated hyperuricemia and renal impairment in rats through upregulation of OAT1 and OAT3
Metal Analysis of Si Wu Tang in Relation to its Clinical Application
No full textSi Wu Tang, a Chinese herbal formula composed of four Chinese herbs (Danggui, Chuanxiong, Baishao and Shudi) was analysed for its magnesium, manganese, zinc, iron and calcium concentrations when made into a decoction. The commonly prescribed amount is 42 g in a specific ratio of the four herbs, and from this it was determined that all metals analysed were within safe limits and daily tolerable limits would not be able to be reached by ingesting this formula (42 g/day) alone. The highest metal in the Si Wu Tang decoction was found to be magnesium at 25 mg/L, which is under the daily intake recommendations. Varying the brewing time during the preparation of the herbs was found to have various effects on the different micronutrients when made up into separate decoctions from 1 g of each separate herb and these observations may be beneficial to Chinese medicine practitioners who wish to vary the Si Wu Tang formula to better suit a patient’s needs. Samples were analysed at least in triplicate and error was found not to be over 15% at the 95% confidence level. Investigating the amount of metals present will increase the understanding of the levels of these beneficial metals and the potential curative effects they provide
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