65,462 research outputs found
Using Affinity Capillary Electrophoresis to Evaluate Average Binding Constant of 18-Mer Diphospho-Tyrosine Peptide to Antiphosphotyrosine Fab
No full textWe used affinity electrophoresis in capillaries to investigate the interaction between a monovalent antiphosphotyrosine antibody fragment, antigen-binding fragment (Fab), and a divalent antigen (dAg), an 18-mer diphosphopeptide phosphorylated on two-site tyrosine residues. The migration shift behavior of Fab in electrophoretic solution was observed and the quantitative expression was presented to estimate the arithmetical average value of the intrinsic affinities for two epitopes on the dAg with the Ag binding site on the Fab. In dAg excess, based on measurement of mobility changes of Fab analytes at different dAg concentrations, the experimental average dissociation constant ( = 27.7 mu m) was calculated, it was also found that the structural variation of the two epitopes for binding specificity to the Ag-binding domain of Fab is not apparent. Moreover, the values of Fab-dAg complexes were measured at higher electric fields and shown to be independent of changes in the electric field. Thus, under conditions where the total dAg concentration is in excess of the total Fab concentration, the method and quantitative expression which we developed is generally useful for the understanding of molecular interaction for an unlabeled monovalent receptor and its divalent ligand in free solution
Immunoaffinity capillary Electrophoresis: Determination of binding constant and stoichiometry for antibody-antigen interaction
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Determination of binding constant of DNA-binding drug to target DNA by surface plasmon resonance biosensor technology
No full textDynamic response of glucagon/anti-glucagon pairs to pulling velocity and pH studied by atomic force microscopy
No full textAn on-Line Quantitative Immunoassay System Based on a Quartz Crystal Microbalance
No full textWe have established a QCM immunoassay system which allows on -line and quantitative monitoring throughout the entire detection procedure and provides information on the surface coverage and the binding ratio of antibody to antigen. Compared to conventional immunoassay systems the QCM system offers advantages of short response times, obviates the need for additive labeling reagents, and permits direct conversion of a frequency signal into mass accumulation. (C) 2000 Elsevier Science B.V. All rights reserve
Energy-Efficient Train Operation: Conclusions and Future Work
No full textThis chapter gives the basic conclusions about energy-efficient train operation covering energy-efficient train driving, energy-efficient train timetabling, regenerative braking, energy storage systems and power supply networks. Future work that will develop energy-efficient train operation further include the interaction of connected driver advisory systems (C-DAS) and automatic train operation (ATO) with railway traffic management systems, cooperative train control in platoons of virtually coupled trains, digital twin technology and particularly its application to power supply systems, and the interaction between the railway network with the electrical power grid and renewable energy generation.Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Transport and Plannin
Relating Research and Teaching: comparing experiences and beliefs
No full textThe relationship between research and teaching has possible benefits and inherent tensions. It is a recurrent topic of discussion by faculty including engineering educators. Exploring a potentially beneficial relationship and is of interest and possible value to engineering faculty, our students, and our stakeholders. Institutions and departments have developed a range of approaches including research-led, research informed, or just plain scholarly. This paper examines the relationship between research and teaching in the undergraduate curriculum. It compares and contrasts evidence of the beliefs and experiences of the engineering faculty and the engineering student. It presents and analyses the result of surveys which gathered qualitative and qualitative data to explore the inter-relationship of research and teaching; in the curriculum; and as it is delivered and experienced in the lab, seminar room and lecture hall. This research builds on existing work developed in a preliminary study which examined ways in which synergies between research and teaching could be achieved, particularly in the ‘hard/applied’ areas of the curriculum. It analyses data from the ‘research-intensive’ and the ‘teaching-intensive’ perspective
Education for Cataloging in Spanish Universities: A Descriptive and Critical Study
No full textObjective: This is a critical descriptive study of the situation of cataloging as an academic discipline within Library and Information Science studies in Spain. Material and methods: The descriptive analysis of the sectional contents of the general and specific guidelines of the degrees of Diplomado (three-year degree) and Licenciado (fiveyear degree) in LIS and the curricular programs of the Spanish university schools or departments. Variables analyzed: The denomination and content descriptors of the course offerings and credit hours. The test-retest method was used, with a qualitative processing of data. Results: General data is given about the studies in LIS: their introduction, the universities that offer them, and the degrees awarded. Cataloging is considered an obligatory core subject matter, and is represented by several courses that present important differences insofar as their denominations, their credits, and their character from one curricular program to the next. The average credit requisite for obligatory courses in cataloging in Spain is 14 (1 credit = 10 class hours), and 19.7 if electives are also considered. At present, this discipline is undergoing a reform that will produce important changes as a result of the adaptation of university studies to the common framework of the European Union
Introduction to Energy-Efficient Train Operation
No full textRailway, as one of the most energy-efficient transport, plays an essential role in improving the world’s energy and environmental sustainability. Statistics about rail share of transport activities and the corresponding energy consumption will demonstrate the energy efficiency of railway and indicate the potential of developing railway transport. Therefore, this chapter will provide an overview of the railway's energy consumption and traffic volume shares. Statistics presented in this chapter show that railway energy consumption decreased in these decades while its transport volume kept stable, and the traffic volume share of the railway is extremely large in urban transport. To achieve the goal of carbon neutralization, the European Union and many countries have conducted research projects on railway energy conservation. The technologies developed in these projects include energy-efficient train driving, integrated timetabling, using regenerative braking energy, etc. A summary of these technologies is also given, along with their potential energy savings, which range from 1 to 25%. This book will analyse and illustrate the whole systems processes of train operation with optimisation solutions. The structure of the following chapters will be presented at the end.Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Transport and Plannin
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