403 research outputs found

    sj-pdf-1-ajs-10.1177_03635465231180323 – Supplemental material for Efficacy and Safety of Stempeucel in Osteoarthritis of the Knee

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    Supplemental material, sj-pdf-1-ajs-10.1177_03635465231180323 for Efficacy and Safety of Stempeucel in Osteoarthritis of the Knee by Pawan Kumar Gupta, Sunil Maheshwari, Joe Joseph Cherian, Vijay Goni, Arun Kumar Sharma, Sujith Kumar Tripathy, Keerthi Talari, Vivek Pandey, Parag Kantilal Sancheti, Saurabh Singh, Syamasis Bandyopadhyay, Naresh Shetty, Surendra Umesh Kamath, Purohit Sharad Prahaldbhai, Jijy Abraham, Suresh Kannan, Samatha Bhat, Shivashankar Parshuram, Vinayaka Shahavi, Akhilesh Sharma, Nikhil N. Verma and Uday Kumar in The American Journal of Sports Medicine</p

    Assessment of somaclonal variation and stability in in vitro regenerated grass pea plants using SDS-PAGE

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    Tissue culture may be one of the possible sources of variation for crop improvement. To assess variation and stability in regenerated plants, shoots were regenerated from the callus derived from axillary explants of 11 grasspea genotypes, and their shoot protein profiles were compared with those of seed cultured plants. The highest response of callus induction (87%) was observed when 2.0 mg/l2,4-dichlorophenoxyacetic acid(2,4-D) and 0.25 mg/l BAP were supplemented in Murashige and Skoog’s (MS) medium. -naphthaleneacetic acid(NAA) and 6-benzylaminopurine(BAP) showed variable redifferentiation response along with callus formation. The MS medium supplemented with 0.5 mg/l BAP showed better multiplication and elongation of shoots.Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed a unique protein band of 43 kDa in both tissue and seed cultured plants of Pusa 24. Polypeptide banding pattern of regenerated plants from 11 grasspea genotypes did not deviate from the banding pattern of parental seed protein. Similarity coefficient values ranged from 0.37 to 0.85 with a mean of 0.43 among the 55 genotypic combinations. Comparison of protein bands between calli raised regenerated shoots and parental seeds revealed the absence of somaclonal variation in regenerated plants, suggesting that the regeneration protocol used in the present study can be used for genomics enabled improvement in grasspea without the risk of additional variation or instability

    Advances in PGPR research/ edited by Harikesh Bahadur Singh, Banaras Hindu University, India, Birinchi Kumar Sarma, Banaras Hindu University, India, Chetan Keswani, Banaras Hindu University, India.

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    Includes bibliographical references and index.This book includes 25 contributions from vastly experienced, global experts in PGPR research in a comprehensive and influential manner, with the most recent facts and extended case studies. Also, the chapters address the current global issues in biopesticide research.Mechanisms of Growth Promotion by Members of the Rhizosphere Fungal Genus Trichoderma / Artemio Mendoza-Mendoza, Guillermo Nogueira-L opez, Fabiola Padilla Arizmendi, Natalia Cripps-Guazzone, Mar ia Fernanda Nieto-Jacobo, Robert Lawry, Diwakar Kandula, Fatima Berenice Salazar-Badillo, Silvia Salas-Mu noz, Jorge Armando Mauricio-Castillo, Robert Hill, Alison Stewart and Johanna Steyaert -- Physiological and Molecular Mechanisms of Bacterial Phytostimulation / Randy Ortiz-Castro, Jes us Salvador L opez-Bucio and Jos e L opez-Bucio -- Real-time PCR as a Tool towards Understanding Microbial Community Dynamics in Rhizosphere / Gautam Anand, Upma Singh, Abhineet Sain, Virendra S. Bisaria and Shilpi Sharma -- Biosafety Evaluation: A Necessary Process Ensuring the Equitable Beneficial Effects of PGPR / Juan Ignacio V ilchez, Richard Daniel Lally and Rafael Jorge Le on Morcillo -- ^Role of Plant Growth-Promoting Microorganisms in Sustainable Agriculture and Environmental Remediation / Rama Kant Dubey, Vishal Tripathi, Sheikh Adil Edrisi, Mansi Bakshi, Pradeep Kumar Dubey, Ajeet Singh, Jay Prakash Verma, Akanksha Singh, B.K. Sarma, Amitava Rakshit, D.P. Singh, H.B. Singh and P.C. Abhilash -- Pseudomonas Communities in Soil Agroecosystems / Betina Cecilia Agaras, Luis Gabriel Wall and Claudio Valverde -- Management of Soilborne Plant Pathogens with Beneficial Root-Colonizing Pseudomonas / Dmitri V. Mavrodi, Mingming Yang, Olga V. Mavrodi and Shanshan Wen -- Rhizosphere, Mycorrhizosphere and Hyphosphere as Unique Niches for Soil-Inhabiting Bacteria and Micromycetes / Elena Voronina and Irina Sidorova -- ^The Rhizospheres of Arid and Semi-arid Ecosystems are a Source of Microorganisms with Growth-Promoting Potential / Fatima Berenice Salazar-Badillo, Silvia Salas-Mu noz, Jorge Armando Mauricio-Castillo, Jorge S aenz-Mata, Artemio Mendoza-Mendoza, Maria Fernanda Nieto-Jacobo and Johanna Steyaert -- Rhizosphere Colonization by Plant-Beneficial Pseudomonas spp.: Thriving in a Heterogeneous and Challenging Environment / Antoine Zboralski, Adrien Biessy and Martin Filion -- Endophytomicrobiont: A Multifaceted Beneficial Interaction / Shatrupa Ray, Vivek Singh, Kartikay Bisen, Chetan Keswani, Surendra Singh and H.B. Singh -- Contribution of Plant Growth-Promoting Bacteria to the Maize Yield / Vivian Jaskiw Szilagyi Zecchin, Angela Cristina Ikeda and Atila Francisco M ogor -- The Potential of Mycorrhiza Helper Bacteria as PGPR / Marieta Marin Bruzos -- Methods for Evaluating Plant Growth-Promoting Rhizobacteria Traits / Antonio Castellano-Hinojosa and E.J. Bedmar -- ^The Rhizosphere Microbial Community and Methods of its Analysis / Mukesh Meena, Manish Kumar Dubey, Prashant Swapnil, Andleeb Zehra, Shalini Singh, Punam Kumari and R.S. Upadhyay -- Improving Crop Performance under Heat Stress using Thermotolerant Agriculturally Important Microorganisms / M.K. Chitara, Chetan Keswani, Kartikay Bisen, Vivek Singh, S.P. Singh, B.K. Sarma and H.B. Singh -- Phytoremediation and the Key Role of PGPR / Elisabetta Franchi and Gianniantonio Petruzzelli -- Role of Plant Growth-Promoting Rhizobacteria (PGPR) in Degradation of Xenobiotic Compounds and Allelochemicals / Deepika Goyal, Janmejay Pandey and Om Prakash -- Harnessing Bio-priming for Integrated Resource Management under Changing Climate / Deepranjan Sarkar, Sumita Pal, H.B. Singh, Ranjeet Singh Yadav and Amitava Rakshit -- ^Unravelling the Dual Applications of Trichoderma spp. as Biopesticide and Biofertilizer / Vivek Singh, Shatrupa Ray, Kartikay Bisen, Chetan Keswani, R.S. Upadhyay, B.K. Sarma and H.B. Singh -- 21 Genome Insights into Plant Growth-Promoting Rhizobacteria, an Important Component of Rhizosphere Microbiome / Vasvi Chaudhry, Niladri Chaudhry and Shrikant S. Mantri -- Plant Growth-Promoting Rhizobacteria (PGPR): Mechanism, Role in Crop Improvement and Sustainable Agriculture / Pallavi Mittal, Madhu Kamle, Shubhangini Sharma, Pooja Choudhary, Devendra Pratap Rao and Pradeep Kumar -- PGPR: A Good Step to Control Several of Plant Pathogens / Laith K. Tawfeeq Al-Ani -- Role of Trichoderma Secondary Metabolites in Plant Growth Promotion and Biological Control / Jyoti Singh, Rahul Singh Rajput, Kartikay Bisen, Surendra Singh and H.B. Singh -- ^PGPR-Mediated Defence Responses in Plants under Biotic and Abiotic Stresses / Gagan Kumar, Jai Singh Patel, Anupam Maharshi, Arpan Mukherjee, Chetan Keswani, S.P. Singh, H.B. Singh and B.K. Sarma.1 online resourc

    Chlorine leak on Mumbai port trust′s Sewri yard: A case study

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    Chemical emergencies involving hazardous chemicals are not uncommon in India. More than 25 incidents have been identified in National Disaster Management Guidelines - Chemical (Industrial) Disaster Management, released in May 2007. In a recent occurrence on the morning of 14 July 2010, nearly at 3:00 a.m., chlorine leak was reported from a gas cylinder referred as turner, weighing about 650 kg, corroding with time at the Haji Bunder hazardous cargo warehouse in Mumbai Port Trust, Sewri, affecting over 120 people in the neighborhood, including students, laborers, port workers and fire fighters, of whom 70 were reported critical. It has been observed to be a blatant case of ignorance and negligence as well as contraventions to the safety and environmental safeguard requirements under existing statues as well as non-maintenance of failsafe conditions at the site requisite for chlorine storage. The analysis revealed significant gaps in the availability of neutralization mechanism and the chlorine stored in open increased the possibility of formation of ingress mixture due to busting of chlorine filled tankers. The Government of India has institutionalized emergency preparedness framework at national, state and district level as envisaged in Disaster Management Act, 2005, to prepare the nation to mitigate such incidences, if all the preventive safety provisions fail. Ministry of Environment and Forests (MoEF) is preparing National Action Plan-Chemical (Industrial) Disaster Management based on National Guidelines to implement all the mechanisms of capacity development across the country

    Study of differences in presentation, risk factors and management in diabetic and nondiabetic patients with acute coronary syndrome

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    Objectives: To compare clinical characteristics, treatment, and utilization of evidence-based medicines at discharge from hospital in acute coronary syndrome (ACS) patients with or without diabetes at a tertiary care cardiac center in India. Methods: We performed an observational study in consecutive patients discharged following management of ACS. We obtained demographic details, comorbid conditions, and cardiovascular risk factors, physical and biochemical parameters, and management. Descriptive statistics are reported. Results: We enrolled 100 patients (diabetics = 28) with mean age of 59.0 ± 10.8 years (diabetics 59.3 ± 11.6, nondiabetics 58.9 ± 8.5). Forty-nine patients had ST-elevation myocardial infarction (STEMI) (diabetics = 14, 28.7%) while 51 had nonSTEMI/unstable angina (diabetics = 14, 27.4%) (P = nonsignificant). Among diabetics versus nondiabetics there was greater prevalence (%) of hypertension (78.6% vs. 44.4%), obesity (25.0% vs. 8.3%), abdominal obesity (85.7% vs. 69.4%) and sedentary activity (89.2% vs. 77.8%), and lower prevalence of smoking/tobacco use (10.7% vs. 25.0%) (P < 0.05). In STEMI patients 28 (57.1%) were thrombolysed (diabetes 17.8% vs. 31.9%), percutaneous coronary interventions (PCI) was in 67.8% diabetics versus 84.7% nondiabetics and coronary bypass surgery in 21.4% versus 8.3%. At discharge, in diabetics versus nondiabetics, there was similar use of angiotensin converting enzyme inhibitors (67.9% vs. 69.4%) and statins (100.0% vs. 98.6%) while use of dual antiplatelet therapy (85.7% vs. 95.8%) and beta-blockers (64.3% vs. 73.6%) was lower (P < 0.05). Conclusions: Diabetic patients with ACS have greater prevalence of cardiometabolic risk factors (obesity, abdominal obesity, and hypertension) as compared to nondiabetic patients. Less diabetic patients undergo PCIs and receive lesser dual anti-platelet therapy and beta-blockers

    Association of low educational status with microvascular complications in type 2 diabetes: Jaipur diabetes registry-1

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    Objective: To determine the association of educational status (ES), as a marker of socioeconomic status, with the prevalence of microvascular complications in diabetes. Methods: Successive patients (n = 1214) presenting to our centre were evaluated for sociodemographic, anthropometric, clinical, and therapeutic variables. Subjects were classified according to ES into Group 1 (illiterate, 216); Group 2 (8.0%) was significantly greater in illiterate (38.0%), low (46.0%) and middle (41.0%) compared to high (31.5%) ES subjects (P < 0.05). Conclusions: There is a greater prevalence of the microvascular disease in illiterate and low ES diabetes patients in India. This is associated with the higher prevalence of smoking/tobacco use, poor quality diet and sub-optimal diabetes control

    Association of low educational status with microvascular complications in type 2 diabetes: Jaipur diabetes registry

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    Objective: To determine the association of educational status (ES), as marker of socioeconomic status, with the prevalence of microvascular complications in diabetes.Methods: Successive patients (n = 1214) presenting to our center were evaluated for sociodemographic, anthropometric, clinical, and therapeutic variables. Subjects were classified according to ES into Group 1 (illiterate, 216); Group 2 (≤ primary, 537), Group 3 (≤ higher secondary, 312), and Group 4 (any college, 149). Descriptive statistics is reported. Results: Mean age of patients was 52 ± 10 years, duration of diabetes 7 ± 7 years and 55% were men. Prevalence of various risk factors was smoking/tobacco 25.5%, obesity body mass index ≥25 kg/m2 64.0%, abdominal obesity 63.4%, hypertension 67.5%, high fat diet 14.5%, low fruits/vegetables 31.8%, low fiber intake 60.0%, high salt diet 16.9%, physical inactivity 27.5%, coronary or cerebrovascular disease 3.0%, and microvascular disease (peripheral, ocular or renal) in 20.7%. Microvascular disease was significantly greater in illiterate (25.9%) and low (23.6%) compared to middle (15.0%) and high (14.7%) ES groups (P .0%) was significantly greater in illiterate (38.0%), low (46.0%), and middle (41.0%) compared to high (31.5%) ES subjects (P < 0.05). Conclusions: There is a greater prevalence of the microvascular disease in illiterate and low ES diabetes patients in India. This is associated with the higher prevalence of smoking/tobacco use, poor quality diet, and sub-optimal diabetes control

    Comparative Study of Heavy Metal Toxicity on Chlorophyll and Carotenoid Levels in Vigna radiata L. Seedlings

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    Rapid industrialization and urbanization have resulted in the contamination of natural resources, particularly water bodies and agricultural soils. This pollution has severely disrupted ecological balance and agricultural productivity, posing a serious threat to global food security. Among the various pollutants, heavy metals such as cadmium (Cd), lead (Pb), and nickel (Ni) are of particular concern due to their high toxicity, persistence in the environment, and tendency to accumulate in plant tissues. This study examines the effects of these heavy metals on the photosynthetic pigments of Vigna radiata L. (mung bean) seedlings, a nutritionally important legume crop. Seedlings were exposed to different concentrations of Cd, Pb, and Ni, and their total chlorophyll and carotenoid contents were measured. The results showed that all three heavy metals significantly reduced pigment levels, with the degree of reduction increasing alongside concentration. At 1000 ppm, cadmium caused the greatest decline in both chlorophyll and carotenoid levels, followed by lead and then nickel, establishing a toxicity order of Cd &gt; Pb &gt; Ni. The substantial decrease in photosynthetic pigments indicates impaired photosynthetic activity, leading to reduced plant growth and productivity. These findings underscore the urgent need for effective monitoring and management of heavy metal pollution to safeguard agricultural systems and promote sustainable food production
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