1,721,040 research outputs found
Studi Q Fever pada Sapi “ Idul Adha” 2014 di Kota Tangerang dengan Metode Imunohistokimia
No full textQuery fever merupakan penyakit zoonosis yang menyebar ke seluruh
dunia. Q fever disebabkan oleh infeksi bakteri Coxiella burnetii. Hewan
yang paling rentan terkena infeksi Q fever adalah ruminansia. Penelitian ini
bertujuan untuk mengetahui kejadian infeksi C. burnetii dan melihat
gambaran histopatologi organ sapi “Idul Adha” 2014 di kota Tangerang.
Metode yang digunakan adalah Random sampling organ hewan dan
pewarnaan Hematoksilin-Eosin serta Imunohistokimia pada sampel.
Penelitian dilakukan sejak bulan Oktober 2014 sampai september 2015 di
Laboratorium Histopatologi, Departemen Klinik Reproduksi dan Patologi,
Fakultas Kedokteran Hewan, Institut Pertanian Bogor. Sampel diambil dari
organ limpa, hati, dan paru-paru di empat tempat pemotongan hewan
Kurban. Hasil penelitian menunjukan terdapat 3 dari 10 sampel yang diuji
imunoreaktif terhadap C. burnetii. Hasil pewarnaan menunjukan terjadi
perubahan histopatologi pada sampel negatif dan positif yang terinfeksi C.
burnetii. Perubahan yang terjadi pada organ limpa berupa deplesi pulpa
putih, peradangan, kongesti, edema dan hemoragi. Perubahan yang terjadi
pada organ hati berupa degenerasi hidropsis, degenerasi lemak, peradangan,
kongesti, hemoragi. Perubahan yang terjadi pada organ paru-paru berupa
emfisema, peradangan, kongesti dan hemoragi
The Effect of Sambiloto, Adas and Sirih Merah Extract on Avian Influenza Virus Infection in Broiler: Histopathology of Liver and Kidney.
No full textThe objective of this research was to study the effect of extract formulation of sambiloto (Andrographis paniculata), adas (Foeniculum vulgare), and sirih merah (Piper crocatum) to inhibit H5N1 avian influenza virus infection in broiler. Active compounds from each herb were andrographolide, anethole, and piperine respectively. Broiler was divided into eight groups, two as negative (no herbal, unvaccinated) and positive (no herbal, vaccinated) control and the other six groups were treated with herbal formula concentration given 5%, 7.5%, and 10% respectively. The six treated groups were split into three vaccinated and the remaining unvaccinated groups. After infected with H5N1 avian influenza virus, sample from each groups were necropsied and liver and kidney were taken to be evaluated histopathologically. Result of this study indicated that the group treated with 7.5% herb and vaccinated showing the mildest histopathological changes in both organs.Penelitian ini bertujuan mengkaji pengaruh pemberian formula ekstrak sambiloto (Andrographis paniculata), adas (Foeniculum vulgare), dan sirih merah (Piper crocatum) dalam menghambat infeksi virus avian influenza H5N1 pada ayam broiler. Komponen aktif yang terkandung dalam masing-masing tanaman obat yakni andrografolid (sambiloto), anetol (adas), dan piperin (sirih merah). Ayam broiler dibagi menjadi delapan kelompok; dua kelompok masing-masing sebagai kontrol negatif (tanpa ekstrak, tanpa vaksinasi) dan kontrol positif (tanpa ekstrak, divaksin); enam kelompok diberi formula ekstrak dengan konsentrasi 5%, 7,5%, dan 10%. Keenam kelompok yang diberi formula ekstrak terdiri atas tiga kelompok divaksinasi, dan tiga kelompok tidak mendapat vaksinasi. Setelah diinfeksi dengan virus avian influenza H5N1, diambil satu sampel dari masingmasing kelompok untuk dinekropsi. Organ hati dan ginjal dari masing-masing sampel dibuat preparat histopatologi lalu diamati dan dievaluasi secara mikroskopis. Hasil penelitian ini mengindikasikan bahwa kelompok perlakuan yang diberi 7,5% formula ekstrak menunjukkan lesio histopatologi yang paling ringan dibandingkan dengan kelompok perlakuan lainnya
Studi Kasus Q Fever Pada Sapi “Idul Adha” Di Wilayah Jakarta Pusat Dengan Metode Imunohistokimia
No full textQ fever merupakan zoonosis yang disebabkan oleh Coxiella burnetii, bakteri
gram negatif, bersifat intraseluler obligat. Hewan ternak dan hewan kesayangan
merupakan reservoir utama infeksi, dan transmisi ke manusia terutama terjadi melalui
inhalasi aerosol. Q fever merupakan penyakit yang ada di seluruh dunia kecuali
Selandia Baru. Q fever memiliki gejala subklinis yang bervariasi dan tidak
patognomonis, sehingga untuk mendiagnosanya perlu dilakukan uji dengan akurasi
tinggi. Penelitian tentang Q fever pada sapi “idul adha” di wilayah Jakarta Pusat dengan
metode imunohistokimia dilakukan dari oktober 2014 sampai september 2015 di Divisi
Patologi FKH IPB. Hasil penelitian menunjukkan 3 sampel imunoreaktif dan 15 sapi
non imunoreaktif terhadap antibodi anti Coxiella burnetii. Hasil pewarnaan HE sampel
positif menunjukkan terdapat lesio pada organ limpa, hati dan paru-paru. Lesio pada
limpa berupa deplesi folikel limfoid, peradangan, dan edema. Lesio pada hati berupa
degenerasi hidropis. Lesio pada paru-paru berupa kongesti, dan hemoragi. Hasil
penelitian ini merupakan laporan kejadian Q fever pada sapi kurban yang pertama kali
dilaporkan di Wilayah Jakarta Pusat
Studi Kasus: Kajian Histopatologi Pada Kematian Seekor Biawak Ambon (Varanus indicus)
No full textThe aim of this case study was to study the cause of death of an Ambon monitor lizard (Varanus indicus) which died suddenly without showing any clinical signs. Organs that were showing abnormality were collected and histopathologically processed. Gross examination showed enlarged liver with multiple white foci ranging from 0.1 to 2 cm in diameter and diagnosed as multifocal abcess. Abcesses were also found in the spleen and kidney. The mucosa of the intestine was filled with hemorrhage exudate and contained many necrotic areas. The intestine was diagnosed as enteritis hemorrhagica et necroticans. The histopathological examination showed granulomatous inflammation in the liver, spleen, kidney and intestine, and amoebas were found in all of these organs. The death of this lizard is caused by liver failure and multifunctional organ failure caused by chronic inflammation of amoebiasis
Studi Kasus Query Fever Pada Sapi “Idul Adha” Di Kota Depok Tahun 2014: Histopatologi Limpa, Hati Dan Paru-Paru
No full textQuery fever atau Q fever merupakan salah satu zoonosis yang menyebar ke
seluruh dunia. Q fever disebabkan oleh infeksi bakteri Coxiella burnetii (C.
burnetii). Ruminansia merupakan hewan paling rentan terkena infeksi C. burnetii.
Penelitian ini bertujuan untuk mengetahui kejadian infeksi C. burnetii dan melihat
gambaran histopatologi organ sapi “Idul Adha” di kota Depok tahun 2014 yang
positif terinfeksi C. burnetii. Metode yang digunakan adalah sampling organ
hewan dan dilanjutkan dengan metode pewarnaan Hematoksilin-Eosin serta
Imunohistokimia. Penelitian dilakukan sejak bulan Oktober 2014 sampai
September 2015 di Laboratorium Histopatologi, Departemen Klinik Reproduksi
dan Patologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor. Sampel
diambil dari organ limpa, hati, dan paru-paru dari 3 tempat penyembelihan hewan
Kurban masing-masing 5 sampel. Hasil penelitian menunjukan terdapat 3 sampel
imunoreaktif C. burnetii dari 15 sampel yang diuji. Hasil pewarnaan HE
menunjukkan terjadi perubahan histopatologi pada sampel yang positif terinfeksi
C. burnetii. Perubahan pada limpa berupa deplesi pulpa putih, peradangan dan
edema. Perubahan pada organ hati berupa degenerasi hidropis, degenerasi lemak
dan peradangan. Perubahan pada paru-paru berupa emfisema, peradangan dan
kongesti. Adanya sampel positif terinfeksi C. burnetii menandakan di kota Depok
terdapat kasus Q fever
Pengaruh Kurkumin terhadap Skleroderma dan Fibrosis Paru-paru Akibat Aplikasi Bleomisin pada Mencit (Mus musculus)
No full textCurcumin is an curcuminoid compound of turmeric. Curcumin has been demonstrated to have anti-oxidant and anti-inflammatory properties. Bleomycin (BLM) is an anti-cancer drug which induced scleroderma and pulmonary fibrosis in human and animals. Here we investigate biological effects of curcumin on bleomycin-induced scleroderma and pulmonary fibrosis in mice through pathomorphological assesment. In this study, each group consisted of 4-6 mice ddy strain, that groups namely (i) control, mice were subcutaneously (SC) injected with 100 μl sterilized aquadest in dorsal skin, (ii) bleomisin (BLM) group, injected with 100 μl of 1 mg/ml BLM SC in dorsal skin, (iii) curcumin (CMN) group, mice were intraperitoneally (IP) injected with 100 mg/kg body weight (BW) curcumin dissolved in 0.5% carboxymethylcellulose (CMC) and injected with 100 μl sterilized aquadest SC, (iv) BLM+CMN group, injected with 100 μl of BLM 1 mg/ml SC and injected with 100 mg/kg BW CMN in 0.5% CMC IP. Injections of PBS, BLM, CMN were performed daily for four weeks period. The mice were euthanized with overdosed ketamine, and afterwards the dorsal skin and lung samples were collected and fixed in 10% buffered neutral formalin (BNF). Histopathological evaluation was performed with hematoxylin-eosin (HE) and Masson’s trichrome (MT) stains. The results showed that BLM treatment significantly decreased hair follicles number and size. Additionally, in BLM-treated group there was significant increase in skin fibrosis area and inflammatory cells (macrophage and lymphocyte) number. In the lung, repeated BLM treatment initiated higher fibrosis area and alveolar wall area fraction as compared with control. In the other hand, CMN treatment results significantly increased hair follicles number and size, significantly reduced skin fibrosis area, lower inflammatory cells number, and significantly decreased fibrosis area, reduced alveolar wall area fraction in BLM-treated mice. Conclusively, our study showed that CMN treatment may inhibit skin and lung fibrogenesis in BLM-induced scleroderma and pulmonary fibrosis
Gambaran Histopatologi bursa Fabricius dan Limpa Ayam Broiler yang Diuji Tantang Virus Avian Influenza H5N1 Setelah Pemberian Ekstrak Sambiloto (Andrographis paniculata Nees).
No full textThis research aim was to study the histopathology of lymphoid organ bursa of Fabricius and spleen of broiler which were challenged by H5N1 AI virus after treatment medicinal plant extract of Sambiloto (Andrographis paniculata Nees). Fourteen chickens were divided into four groups with different treatment that are K1 (without the extract of Sambiloto, without the AI virus infection), K2 (without the extract of Sambiloto, infected with AI virus), P1 (given the extract of Sambiloto without the AI virus infection), and P2 (given Sambiloto extract and infected with AI virus). Bursa of Fabricius and spleen were taken for histopathological preparations, and then were observed using light microscopy. Histopathological observations showed that the H5N1 AI virus infection causes oedema, necrotic, and depletion of lymphoid follicles on the bursa of Fabricius; and congestion, hemorrhage, and depletion of lymphoid follicles in the spleen. The treatment of extract of Sambiloto before H5N1AI virus infection can reduce lesion in the bursa of Fabricius and spleen. Sambiloto extract indicated potentially inhibit the infection of H5N1 avian influenza viruses
Kajian Histopatologi Limpa dan Jantung Sapi yang Terinfeksi Coxiella burnetii di Rumah Potong Hewan Terpadu Kota Bogor
No full textQuery fever is a zoonotic disease caused by Coxiella burnetii. This agent infects any kinds of animal including cattle. In many cases report, there are no clinical signs appear during the infection of C. burnetii. Then, it is also difficult to diagnose this disease. The study about infection of C. burnetii in cattle was carried out from February to October 2013 at Bogor Slaughterhouse. The aim of this study was to know the infection of C. burnetii and compare the histopathological finding of spleen and heart of infected cattle. A total of 50 samples of cattle spleen, heart, liver, kidney, and lung were collected, and processed for immunohistochemical and hematoxyllin and eosin staining. The result of immunohistochemical staining showed 5 samples were immunoreactive to polyclonal rabbit anti-Coxiella burnetii antibody FKH IPB in the spleen, liver, and kidney. The histopathological finding indicated hemosiderin pigment deposits, lymphoid follicle depletion, infiltration of lymphocyte, macrophage, neutrophil, and eosinophil cells, and oedema. No lesion appeared in heart with positive infection of C. burnetii, however myocardial degeneration, myocardial regeneration, and myocardial congestion were found in heart with negative infection of C. burnetii
Studi histopatologi organ limpa hamster (Mesocricetus auratus) yang Diinfeksi Coxiella burnetii
No full textQuery fever is a zoonosis caused by Coxiella burnetii (C. burnetii). The aim of this research was to determine the histopathological changes of spleen of hamster (Mesoscricetus auratus) which were infected by C. burnetii using Haematoxylin-Eosin (HE) and Immunohistochemistry (IHC) methods. This research used 6 hamsters which were devided into two passage. The histopathological examination picture with HE staining of first passage in hamsters showed relatively similar results with those the second passage such as red pulp hemorrhage and infiltration of inflammatory cells. Histopathological observation of spleen from first passage hamster by using IHC staining showed the positivity was unclear compared to the second passage hamster
Gambaran Histopatologi Organ Hati dan Ginjal Setelah Pemberian Ekstrak Ethanol Sirih Merah dan Diuji Tantang Virus AI H5N1
No full textThe objective of this study was to determine the potency of Piper crocatum ethanolic extract (PC) to prevent virus infection, and to describe the liver and kidney histopathology of broiler after challenged by H5N1 virus. There were four group treatments in this study, such as K1 (Positive Control), K2 (Negative Control), P1 (Chickens PC treatment and challenged by H5N1 virus), and P2 (Chicken PC treatment). The histopathological data was processed by Kruskall Wallis statistical analysis followed by Dunn test. Based on mortality data, PC in a single composition capable to reduce mortality up to 62.5%. Histopathological lesion that appeared in liver and kidney were congestion, cell degeneration, and necrose. It can be concluded that PC able to prevent the death of chicken due to infection of H5N1 virus, however remain lesion in the liver and kidney
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