23 research outputs found
Chinese literary works translated into Baba Malay: a bibliographical study
No full textAnalyses 68 unique titles of Baba translated works published between 1889 and 1950. The titles are held in the libraries of the University of Malaya (UM), Science University Malaysia (USM), National University of Malaysia (UKM), the Dewan Bahasa dan Pustaka (DBP), National University of Singapore (NUS), National Library of Singapore (NLS) and the British Library (BL). The results reveal three periods of active publication of Baba translated works. A total of 18 works were translated before World War I, followed by 10 just after the war, 39 titles were published before the break of the World War II and 1 was identified in 1950. There were 103 persons involved in the 68 translated works, some of whom are responsible for more than one title. The most prominent translators were Chan Kim Boon, Wan Boon Seng, Seow Chin San and Lee Seng Poh. Some of the translators were also be editors, illustrators or editors. There were 31 publishers and 21 printing presses involved, all were located in Singapore. The most active publishers were Wan Boon Seng, Kim Seck Chy Press and Nanyang Romanised Malay Book Co. The translated works mainly cover historical classical Chinese stories, chivalrous stories, romances, folklore and legends. The titles were priced between 10 cents to 2 dollars in Straits currency. The University of Malaya Library held the largest number of unique title (62) out of which 15 were unique titles
Developing a long-term microfluidic system to study the mesoscopic dynamics of Piezo1 activity
No full textThe cellular microenvironment (or cell niche) is a complex and specialised environment, consisting of physical, chemical, and biological factors that collectively influence the cells residing within. Interactions within this dynamic microenvironment play a critical role in regulating and maintaining cellular activities and function. Within the cellular microenvironment, resident cells constantly experience various mechanical force stimuli, which previous in vitro studies cannot fully recapitulate. Hence, the inclusion of mechanical forces (for example fluid shear stress) in the cell culturing system would yield a more physiologically relevant investigation of cellular behaviour.
Cells commonly express mechanosensitive proteins and receptors to sense and transduce external mechanical stimuli to respond appropriately. A widely expressed mechanosensitive ion channel, Piezo1, has been shown to play an important role in cellular mechanotransduction. Our lab previously generated a genetically-encoded Piezo1 sensor (GenEPi), which allows precise detection of Piezo1-specific activities. To investigate the activities of Piezo1 in near-physiological conditions, I developed a microfluidic-based system to study the dynamics of Piezo1 in mammalian cells using our GenEPi sensor.
Using my developed system, I discovered that Piezo1 clusters exhibit more directed diffusive motion after fluid shear stress. By studying the dynamics of the protein, I revealed differential Piezo1 responses across fluid shear stress magnitudes. I also reported a mechanical threshold to HEK-Piezo1 activation during constant fluid shear stress. Through the GenEPi dynamic responses, I observed that cellular Piezo1 activations are synchronised to mechanical shear triggers but become increasingly asynchronous under sustained shear stress. Crucially, the characteristics of Piezo1’s dynamic within cells reported here will significantly advance our understanding of the interplay between mechanical forces and cellular behaviour within the cellular microenvironment. This system can also be a model for others to adopt in investigating the dynamics of other mechanosensitive proteins expressed in the cells.Open Acces
MicroRNA Regulation in Infectious Diseases and Its Potential as a Biosensor in Future Aquaculture Industry: A Review
No full textAn infectious disease is the most apprehensive problem in aquaculture as it can lead to high mortality in aquatic organisms and massive economic loss. Even though significant progress has been accomplished in therapeutic, prevention, and diagnostic using several potential technologies, more robust inventions and breakthroughs should be achieved to control the spread of infectious diseases. MicroRNA (miRNA) is an endogenous small non-coding RNA that post-transcriptionally regulates the protein-coding genes. It involves various biological regulatory mechanisms in organisms such as cell differentiation, proliferation, immune responses, development, apoptosis, and others. Furthermore, an miRNA also acts as a mediator to either regulate host responses or enhance the replication of diseases during infection. Therefore, the emergence of miRNAs could be potential candidates for the establishment of diagnostic tools for numerous infectious diseases. Interestingly, studies have revealed that miRNAs can be used as biomarkers and biosensors to detect diseases, and can also be used to design vaccines to attenuate pathogens. This review provides an overview of miRNA biogenesis and specifically focuses on its regulation during infection in aquatic organisms, especially on the host immune responses and how miRNAs enhance the replication of pathogens in the organism. In addition to that, we explored the potential applications, including diagnostic methods and treatments, that can be employed in the aquaculture industry
Author Correction: Increased Serum Hyaluronic Acid and Heparan Sulfate in Dengue Fever: Association with Plasma Leakage and Disease Severity (Scientific Reports, (2017), 7, 1, (46191), 10.1038/srep46191)
No full text10.1038/s41598-021-92487-3Scientific Reports1111334
Non-Invasive Multimodality Imaging Directly Shows TRPM4 Inhibition Ameliorates Stroke Reperfusion Injury
No full textThe transient receptor potential melastatin 4 (TRPM4) channel has been suggested to play a key role in the treatment of ischemic stroke. However, in vivo evaluation of TRPM4 channel, in particular by direct channel suppression, is lacking. In this study, we used multimodal imaging to assess edema formation and quantify the amount of metabolically functional brain salvaged after a rat model of stroke reperfusion. TRPM4 upregulation in endothelium emerges as early as 2 h post-stroke induction. Expression of TRPM4 channel was suppressed directly in vivo by treatment with siRNA; scrambled siRNA was used as a control. T2-weighted MRI suggests that TRPM4 inhibition successfully reduces edema by 30% and concomitantly salvages functionally active brain, measured by F-FDG-PET. These in vivo imaging results correlate well with post-mortem 2,3,5-triphenyltetrazolium chloride (TTC) staining which exhibits a 34.9% reduction in infarct volume after siRNA treatment. Furthermore, in a permanent stroke model, large areas of brain tissue displayed both edema and significant reductions in metabolic activity which was not shown in transient models with or without TRPM4 inhibition, indicating that tissue salvaged by TRPM4 inhibition during stroke reperfusion may survive. Evans Blue extravasation and hemoglobin quantification in the ipsilateral hemisphere were greatly reduced, suggesting that TRPM4 inhibition can improve BBB integrity after ischemic stroke reperfusion. Our results support the use of TRPM4 blocker for early stroke reperfusion.sponsorship: This work was supported by grant NMRC/1283/2011 from the
Singapore Ministry of Health’s National Medical Research Council to
P.L. and funding from the Singapore Bioimaging Consortium, Agency
for Science, Technology and Research (A*STAR) to J.G. (Singapore Ministry of Health’s National Medical Research Council|NMRC/1283/2011, Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR))status: Publishe
Publication productivity of Malaysian researchers in the field of Computer Science and Information Technology
No full textSearches made in the CD-ROM databases, COMPENDEX (1987-1999), IEL (IEE/IEEE Electronic Library) (1988-1999) and INSPEC (1990-1998) revealed a total of 389 publications contributed by Malaysian researchers in the field of computer science and information technology. The trend in output indicates rapid growth that is expected to continue in future. A total of 458 unique Malaysian authors contributed to the 389 publications. Collaboration between two authors was the dominant authorship pattern. Single-authored or more than 3-authored works were rare. The active authors were affiliated to a few institutions, with the Universiti Teknologi Malaysia, Universiti Sains Malaysia and Universiti Malaya accounting for the highest number of publications, either in the form of journalarticles or papers in conference proceedings. The most active research areas include simulation system, control engineering, computer-assisted instruction, programming techniques, expert systems, asynchronous transfer mode, image processing, software engineering and digital signal processing and applications
An inclusion initiative in Singapore for preschool children with special needs
No full textThis paper describes a preschool inclusion initiative in Singapore, which currently has no mandate for integrating children with special needs in mainstream schools. This very small-scale qualitative study involving children with mild learning disabilities discusses a therapy outreach programme by a local children’s hospital. It explores the supports and challenges of this experience based on interviews with therapists, teachers, principals, and parents. Facilitators of inclusion included communication, collaboration, availability of training and resources, and a readiness for inclusion. Barriers to inclusion included person-related hindrances, structural obstacles, gaps in program delivery, and limited specialized training and resources. We learned that in the absence of mandatory provisions for inclusion, children with special needs can be supported in regular education when there is “buy in” for early inclusion and intervention amongst key stakeholders. Practical strategies toward this end are discussed.Accepted versio
Author Correction: Highly specific and non-invasive imaging of Piezo1-dependent activity across scales using GenEPi
No full textCorrection to: Nature Communications, published online 19 July 2023 In the original version of this article, the given and family names of Armando Del Rio Hernandez were incorrectly structured. The name was displayed correctly in all versions at the time of publication. In this article the affiliation details for Nordine Helassa were incorrectly given as ‘7. Centre for Cardiovascular Science, Department of Cardiovascular Science and Metabolic Medicine, Institute of Life Course and Medical Sciences, Faculty of Health and Life Sciences, University of Liverpool, Liverpool, UK.’ but should have been ‘7. Department of Biochemistry, Cell and Systems Biology, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool, UK.’. The original article has been corrected
Figure 6 from Patterns of Oncogene Coexpression at Single-Cell Resolution Influence Survival in Lymphoma
No full textEvaluation of M+2+6− cells in scRNA-seq datasets of DLBCL. A, Uniform Manifold Approximation and Projection (UMAP) of malignant B cells from the Roider and Steen cohorts. B, Proportion of subpopulations across samples and annotation of M+2+6− cells in UMAP. C, Correlation of genes enriched in the M+2+6− subpopulation as evaluated by scRNA-seq with hits from the bulk GEP cohorts (Fig. 5E). CCND2 is highlighted and is among the concordant hits (see also Supplementary Table S13). D, WikiPathways terms enrichment among genes positively associated with M+2+6− cells. Both axes in C and D are scaled exponentially for clarity (see also Supplementary Table S14).</p
Figure 2 from Patterns of Oncogene Coexpression at Single-Cell Resolution Influence Survival in Lymphoma
No full textPrognostic significance of subpopulations after R-CHOP therapy. A, Pooled univariate Cox PH model analysis for MYC, BCL2, and BCL6 single oncogene and subpopulations percentage extent as predictors of OS across multiple DLBCL cohorts. Percentage extent was used as a continuous variable in the model at 5% increments (see Survival Analysis) for an unbiased comparison between the variables. Pooled P values were Bonferroni corrected for single oncogenes and subpopulations independently to adjust for multiple testing and are shown for each variable. Hazard ratio (HR) with 95% confidence interval (CI) per 5%-positivity increment is shown (see also Supplementary Table S4). B, Kaplan–Meier OS analysis of dichotomized into M+2+6− high and low groups. Log-rank test, shading denotes 95% CI. An optimal dichotomization cutoff was used for stratification; total patient numbers in each group are shown.</p
