3 research outputs found
EDITORIAL: Functional Food Products & Ingredients For Gut Health
No full textGUEST EDITOR'S COMMENTAR
Microencapsulation of L. acidophilus NRRL B-4495 in whey Protein-Pullulan Microparticles: Influence of Pullulan Concentration and Outlet Temperature
No full textSpray drying technique is one of the oldest methods adapted to many industrial areas to protect bioactive components. In this study, pH and heat tolerance of encapsulated probiotic Lactobacillus acidophilus NRRL B-4495 cells were investigated. Additionally, influence of process conditions including outlet temperature and pullulan concentration on spray drying process was observed. Scanning electron microscopy images showed that incorporation of pullulan higher than 2.0 % in wall matrix created huge amount of fibrous particles. Spherical microcapsules having smooth surface were formed with 2.0 % pullulan (WPI-pullulan4.5:1) formulation leading to an improvement of barrier properties of microcapsules. Moreover, incorporation of pullulan improved the survival rate to 94.21 % after spray drying. Results suggested that decreasing outlet temperature exhibited much higher cell survivals up to 92.68 %. However, between outlet temperatures, significant differences (p≤0.05) in moisture content and recovery of final product indicated that more effective encapsulation of L. acidophilus NRRL B-4495 cells was achieved at 50 °C. During spray drying, due to dehydration and high heat, cytoplasmic membrane of bacterial cells undergo damage and therefore, microencapsulation in WPI-pullulan blend by spray drying provided the highest survival against heat stress at 45 ºC. Moreover results showed that encapsulated cells survived at minimum desired level (7 log CFU/g) at pH 2.0 in contrast to free cells
Developing a functional lozenge with microencapsulated Lactiplantibacillus pentosus to improve oral and dental health
No full textIn this study, a functional lozenge was developed by using microencapsulated Lactiplantibacillus pentosus NRRL-B 227. Antimicrobial activity of L. pentosus was determined to find out the efficiency on cariogenic and pathogen microorganisms that are known to cause dental caries and gum diseases. Streptococcus mutans is a well-known cariogenic strain, which easily converts sugars to acids and exert adverse effects on dental health. Candida albicans is an opportunistic pathogen when body resistance is weak, it becomes dominant in the mouth, causing disruption of oral health. Within this context, by applying several methods (e.g. broth microdilution, disc diffusion, agar overlay and planktonic culture assays), L. pentosus showed antibacterial and antifungal activities against S. mutans ATCC 25175 and C. albicans DSMZ 5817, respectively. Thus, three different lozenge formulation were produced such as; CL control formulation without cells, CPL formulation containing micro encapsulated cells and FPL formulation containing free cells. The microbiological, physicochemical and sensorial studies were carried out for all formulations stored at 4 degrees C and 25 degrees C. Among these, CPL was found to be more stable than FPL, since microencapsulation with Pullulan/WPC water-in-oil emulsion play a protective role. L. pentosus viability has been lost after 1 month at 25 degrees C, however the viability was maintained without significant reduction at 4 degrees C in case of CPL samples. In the light of findings, lozenge formulation incorporated with L. pentosus can be considered as potential antimicrobial to improve oral health and therefore may become a promising candidate to contribute to the range of functional food products
