4,437 research outputs found
Interview of Thomas Scott Sutton by Robert B. Sutton
No full textDean Rummell: Dean of the College of Agriculture (pp. 1-3) --
M.S. Randhawa: Agricultural Research in India (pp. 2, 10) --
P.N. Thapar: Ministry of Agriculture in India (p. 6) --
Ronald Thompson: member of OSU research team in India (p. 7)This interview describes a cooperative program started in 1954 between OSU's College of Agriculture and the state of Punjab, India. Over fifteen years, the program resulted in limited successes, including a wider perspective on international cooperatio
Scott Sutton
No full textPhotograph used for a story in the Daily Oklahoman newspaper. Caption: "Scott Sutton buries his head on his towel as he is comforted by a teammate at he final seconds of Sunday's loss to Tulsa in the NCAA Midwest Regional Tournament.
Fairground Organ at Sutton Coldfield
No full textPat Collins' Wonderland stand alone Marenghi organ photographed at Sutton Coldfield, 1950. Digitisation and record funded by the Pilgrim Trust
Limiting Avoidable Microbiological Variability
No full text[ "Microbiology Topics" discusses various topics in microbiology of practical use in validation and compliance. We intend this column to be a useful resource for daily work applications. Reader comments, questions, and suggestions are needed to help us fulfill our objective for this column. Case studies from readers are most welcome. Please send your comments and suggestions to column coordinator Scott Sutton at scott.sutton@microbiol. org or journal coordinating editor Susan Haigney at [email protected]. KEY POINTS The following key points are discussed in this article: • Quality control (QC) microbiology test data are subject to significant variability, both avoidable and unavoidable • Good microbiological procedures, backed by sound microbiological practices, can serve to minimize avoidable variability • The lab's standard operating procedure (SOP) system is a powerful tool to describe and document compliance with good practice • The lab should determine critical areas of coverage for the SOP system to ensure a comprehensive program • The SOP for a lab test should describe critical parameters of the test and meet the criteria of regulatory requirements and guidance for that procedure. The documentation of compliance with these requirements is both a legitimate good manufacturing practice (GMP) audit concern and a useful source of information for investigations. • A sound SOP system can serve to minimize avoidable variability in the microbiology lab • SOPs may be categorized into testing methods, documentation and SOPs, environmental monitoring, and laboratory support activities • Training for the members of the lab should be tightly tied to the SOP system, and can support functional specialization of staff • SOPs for each functional area are described • The content of this discussion should serve to benchmark your system, guide regulatory compliance, and be a framework for training • Considering the SOP system from a functional perspective links job skills to SOPs and facilitates tracking of revisions • Controlling variability and avoidable error is critical to successful microbiology laboratory operation because microbiology is exquisitely sensitive to personnel performance and techniques. INTRODUCTION Microbiology in the QC laboratory is subject to variability in the test results, in the samples taken, in the manner in which they are taken (with severe limitations in sample size contributing to the problem), and Limiting Avoidable Microbiological Variability Scott Sutton ABOUT THE AUTHOR Scott Sutton, Ph.D., is owner and operator of The Microbiology Network (www.microbiol.org), which provides services to microbiology-related user's groups. Dr. Sutton can be reached at scott. [email protected]. g x p a n d j v t . c o
Rowland Scott photograph, Sutton Coldfield, 1953.
No full textPat Collins' Razzle Dazzle centre photographed 18 July 1953. Scott negative number 1096. See also 178C57.1060
Rowland Scott photograph, Sutton Coldfield, 1952.
No full textPat Collins' Savage engine with Steam Yachts - SY16 - photographed 16 April 1952. Scott negative number 842
Rowland Scott photograph, Sutton Coldfield, 1949.
No full textPat Collins' Band Wagon photographed 19 April 1949. Scott negative number 389
Antony Sutton statement to the 1972 Republican Platform Committee
No full textStatement by author and professor of economics Antony Sutton to the Platform Committee at the 1972 Republican National Convention. His statement regards the backwards state of Soviet technology and his recommendations on trade policy
Showmen at Sutton Coldfield
No full textPhotograph of showmen John J. Collins and William Bagnall standing in front of Pat Collins Scenic Railway at Sutton Coldfield, 28 May 1950. Digitisation and record funded by the Pilgrim Trust
Microbiology Topics. ABOUT THE AUTHOR Measurement of Microbial Cells by Optical Density
No full text"Microbiology Topics" discusses various topics in microbiology of practical use in validation and compliance. We intend this column to be a useful resource for daily work applications. Reader comments, questions, and suggestions are needed to help us fulfill our objective for this column. Please send your comments and suggestions to column coordinator Scott Sutton at scott. [email protected] or journal coordinating editor Susan Haigney at [email protected]. KEY POINTS The following key points are discussed: Quality control (QC) microbiology tests require controlled levels of inocula and require fresh preparations of cells for those inocula The concentration of cells in a suspension can be estimated by optical density, but this must be confirmed by plate count The optical density readings against cell mass are specific to the microorganism species The qualification of these readings must be confirmed after major maintenance to the bench top spectrophotometer (e.g., after replacement of the bulb). There are, of course, two problems with these instructions. The first is that the technician is instructed to use an inoculum of about 10 8 microorganisms per milliliter and then instructed to determine this by plate count. Colony forming units (CFU) and cells (micro-organisms and spores) are different measures. This will inevitably lead to difficulties as the unfortunate lab worker cannot guarantee the number of cells in the suspension, only the number of CFU found. However, we can accept the scientific inaccuracy, as the numbers will generally work out. The more serious problem is the instruction to use the plate count CFU for determination of the inoculum for the test, and that the suspension shall be used immediately. This quite frankly cannot be done. If you use the suspension immediately, the plate counts are unavailable; if you use the plate counts to set the inoculum, then the suspension is at least a day old. DETERMINATION OF INOCULUM FOR THE AET Contrast these instructions with those in the United States Pharmacopeia (USP) (2) for the same exercise: Scott Sutton are needed to help us fulfill our objective fo fo for this column. Please send your comments an an and d d su u ugg gg gges es etions to column coordinator Scott Su Su Sutt tt tton n n at sc sc scot ot ott. t. t. [email protected] or journal co co coor o o dina a nating e e editor Susan Haigney at shaigney@ y@ [email protected]. KEY POINTS suspending fluid … Add sufficient suspend fluid to reduce the microbial count to about micro-organisms per milliliter…Remove imm ately a suitable sample from each suspension d d de d termine the number of colony-forming u per milliliter in each suspension by plate coun membrane filtration (2.6.12). This value se KEY POINTS The following key points are discuss sed ed: Quality control (QC) microbiolog gy test sts s re equ quire controlled levels of inocula and nd r re equi ire f fresh sh preparations of cells for those inocula The co o onc ncen en ntr tr trat at atio io ion n n of cells in n a a su uspen nsi sion o can be es s sti i imated b b by y y op op opti ti tical dens nsity, y, b but ut this s mu must s be membrane filtration (2.6.12). This value se to determine the inoculum and the baselin use in the test. The suspensions shall be u immediately." There are, of course, two problems with these t t tion n ns. s. s. T T The he he f f fir ir irst st i is s s th th hat at t t th h he t t tec e echnicia an is is i ins nstr truc u te an an an ino no noculum of a a abo out 10 0 0 8 8 8 m m mic croorg gan nisms s pe per m c co conf f fir rmed by p p plate te te c c cou o o nt t Th h he op p ptical den n nsit t ty r re rea adin ings gs a agai inst st cel e l m mass a are r s s sp s ec ec ec e ifi i ic to th h h he e e e m m mi m croo o o org gan an anis is sm specie ie es Th Th Th he q qu l l alif if ifi i ication of the h h se readings mu t t st be confirmed e e e a a a after m m m maj j j jor o o mai ai aint t ten e e ance ce ce t t to o o the e e be b b nc c ch h h t t top sp sp sp spec ec e ect t tropho ho ho hoto to to tom m meter r (e (e (e.g g g., aft fter er er r repla ace ement nt nt of the bulb) a a and d d th th then n n i i instru u ucte e ed to o o d d deter erm mine e thi his s by by plat C C Colony f f fo o orm m ming un n nits ( ( (CF C CFU) U) a and nd ce ells s ( (mi mic cro-or an an and d d d sp p por r res es es es) ) ) ) are d d dif f ffer er er ere e en e t measu ur u es es es. T This w w wil ill l l in l l lead d d d t t t to o o di di di dif fficulties as t t the unfortun t t ate lab wo k k rke guar r r ran a a a tee th th th he e e e numb mb mb ber of f f f ce ce ce cell ll lls s in t t the he he s s sus u u pens ns nsi i i the e e nu nu nu numb m m m er o o of CFU U U foun n n nd d. d. d H H Ho ow o ev ver er r, , w w we can n n a a ac scientific inaccuracy as the numbers will genera of the bulb) ) ). DE DE DE DETE TE TE TERM RM RM RMIN IN IN INAT AT AT ATIO IO IO ION N N N OF OF OF OF I I INO NO NOCU CU CULU LU LUM M M FO FO FOR R R scientific inaccuracy, y as the numbers will g genera ou ou ou out. t t t Th Th Th The e e e mo mo mo more re re re s s ser er er erio io io ious us us us p p p pro r ro robl bl bl blem em em i i is s s th th the e e in in inst st stru ru ruct ct ctio o th th th the e e e pl pl pl plat at at a e e e e co co co coun un un unt t t t CF CF CF CFU U U U fo fo fo for r r r de de de dete te te t rm rm rmin in inat at atio io ion n n of of of t t the he he i i in
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