1,721,010 research outputs found
Biodiversity of the genus Trichoderma and identification of marker genes involved in the antagonism between Trichoderma spp. and plant pathogenic fungi
Full text linkAs Mediterranean islands are known for their high diversity of vascular plants and a high
level of endemism, they can provide basic insights into the relationship between
geographical patterns and ecological processes. Studying the soil fungus
Trichoderma/Hypocrea is of special interest to our working group due to its world-wide
distribution, the continuos ongoing identification of new isolates, and its high percentage of
strains being effective as biocontrol agents.
We isolated 482 strains of Hypocrea/Trichoderma from 15 soils comprising undisturbed
and disturbed soil environments. Isolates were identified at the species level by the
oligonucleotide BarCode for Hypocrea/Trichoderma, sequence similarity analysis and
phylogenetic inferences. The majority of the isolates were positively identified as pan-
European and/or pan-global Hypocrea/Trichoderma species from Sections Trichoderma
and Pachybasium.
T. harzianum (H. lixii) is well known as a biocontrol agent against a range of
economically important plant pathogens. In the present study we used a Rapid Subtraction
Hybridisation approach to isolate genes which are differentially expressed during mycelial
contact between T. harzianum and R. solani, and could serve as marker genes for
selection of superior biocontrol strains. The 50 characterised clones comprised proteins
involved (i) in protein synthesis and turn-over, (ii) in metabolism, and (iii) in the hydrolysis
of macromolecules. An acetyl xylane esterase and an endoglucanase showed significant
upregulation during in vivo confrontation of a T. harzianum strain with high antagonistic
capability towards R. solani, while the expression was low or absent in a T. harzianum
strain with poor antagonistic activity
Analysis of the interaction of trichodiene synthase 5 (TRI5) with natural and natural-like inhibitors of trichothecene biosynthesis
No full textThe fungal pathogen Fusarium culmorum causes Fusarium head blight (FHB) on cereals, resulting in yield loss and contamination of grain with type B trichothecene mycotoxins. A key step in the synthesis of trichothecenes is catalyzed by the trichodiene synthase 5 (TRI5) that converts farnesyl pyrophosphate to trichodiene. Ferulic acid proved an efficient inhibitor of type B trichothecene biosynthesis and TRI5 gene expression in Fusarium liquid cultures. In this work several natural and natural-like compounds belonging to phenol and hydroxylated biphenyl structural classes were tested in vitro to determine their inhibitory activity towards TRI5. The recombinant TRI5 was expressed in E. coli, and the interaction with different inhibitors was analyzed by Surface Plasmon Resonance (SPR). The screening of inhibitors was performed in both direct and competitive binding format to determine which inhibitors could compete with the binding of farnesyl pyrophosphate to the enzyme active site. A combination of inhibition kinetics and computational modeling of interacting-structures may facilitate the testing of novel potential TRI5 inhibitors and the prediction of their inhibitory mechanism
Valutazione della efficacia di un vettore di silenziamento genico per modulare l'espressione dei geniTRI6eTRI5coinvolti nella biosintesi del deossinivalenolo nel fungo fitopatogeno fusarium culmorum
Full text linkLo scopo di questo studio è stato
quello di analizzare l’espressione differenziale di geni preposti alla biosintesi dei tricoteceni
(TRI5 e TRI6) e valutare la produzione di micotossina (DON e il suo derivato acetilato 3Acetil
DON) in un ceppo selvaggio di Fusarium culmorum e in ceppi mutanti generati per inserzione di
un vettore di silenziamento genico
Fusarium species associated with head blight and foot and root rot on durum wheat in Sardinia, Italy: results from a 12-year survey
No full textWater distribution systems in Sardinian hospitals host invasive clonal lineages of the Fusarium oxysporum and Fusarium solani species complexes
No full textA novel Fusarium culmorum gene involved in foot and root rot on durum wheat identified by trasposon-mediated insertional mutagenesis
No full textMolecular identification of Trichoderma species associated with green mould disease of Pleurotus spp. in Sardinia
No full textStraightforward preparation of biologically active dimers of ferulic acid
Full text linkFerulic acid (4-hydroxy-3-methoxycinnamic acid) is an ubiquitous phenolic compound in plant tissues, and it constitutes a naturally occurring antioxidant
compound found in many foods. Here we present different convenient ways to obtain five naturally occurring DiFAs via
peroxidase-mediated oxidative coupling reactions or classic cross-coupling oxidations through a sustainable, safe and inexpensive chemistry. Such compounds arise from 5-5’,
8-8’, 8-5’ and 8-O-4’ coupling and some of them have been tested as potential inhibitors of micotoxin productionin vitro
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