1,720,975 research outputs found
Processing of herpes simplex virus-1 glycans in cells defective in glycosyl transferases of the golgi system: Relationship to cell fusion and virion egress
No full textWe studied herpes simplex virus-1 (HSV-1) glycan structure and the expression of HSV-1 functions regulated by viral glycoproteins in Ric21 cells (P. Vischer and R. C. Hughes, Eur. J. Bioch. 117, 275-284, 1981). This is a line of ricin-resistant mutant BHK cells defective in the enzymes of the Golgi system which add terminal sugars to N-linked glycans. Twp kinds of alterations were observed in the glycosylation of HSV glycoproteins in Ric21 cells. First, there was a defective processing of complex glycans leading to a reduction of biantennary and triantennary species and an increase of incompletely processed monosialylated oligosaccharides. Second, there was an overall reduction in the accumulation of HSV-1 glycoproteins. We found that (i) the release of herpesvirions from Ric21 cells was markedly lower than that from BHK cells, possibly reflecting reduced terminal sugar addition which, in turn, might affect the intracellular transport of glycoproteins. (ii) HSV-1 (MP)-infected Ric21 cells fused with a low efficiency. Furthermore, polycaryocytosis was reduced or abolished in BHK and in Ric21 cells exposed to neuraminidase, indicating that the presence of sialic acid residues in the cell surface glycans is essential for cells to interact in a fashion that brings cell fusion. (iii) Although capsid assembly was comparable, the rate of accumulation of infectious virus decreased in Ric21 cells. Infectivity of released virions from Ric21 and BHK cells was similar, in agreement with previous studies showing that complex-type glycans do not appear to be required for herpesvirion infectivity. The decrease in infectious HSV-1 yield seems to correlate with overall reduced ability to synthesize glycoproteins. © 1983
Emamectina, nuovo formulato contro carpocapsa di melo e pero
No full textNel periodo 2014-2016 sono state condotte in Trentino ed Emilia-Romagna diverse prove finalizzate a valutare l' efficacia di un nuovo formulato a base di emamectina benzoato (Affirm Opti) nei confronti della carpocapsa del melo e del pero. Il nuovo prodotto ottenuto con la tecnologia «pepite» ha garantito un' efficacia equivalente al formulato sino a oggi in commercio (Affirm), permettendo al contempo di dimezzare la quantità di sostanza attiva distribuit
VALUTAZIONE IN SEMI-CAMPO DELLA PERSISTENZA DEI PRINCIPALI FUNGICIDI UTILIZZATI NEL CONTENIMENTO DELLA MACULATURA BRUNA DEL PERO
No full textThe persistence of the activity of old (thiram, dodine, captan, fluazinam, fludioxonil, boscalid) and newly marketed fungicides (fluopyram, penthiopyrad, fluxapyroxad) for the control of brown spot of European pear was evaluated in three semi-field experimental trials over the years 2013-15. Potted pear plantlets were treated 10, 7 and 5 days before the artificial inoculation with a Stemphylium vesicarium conidial suspension. Severity and incidence of brown spot on leaves were evaluated after 4-5 days of incubation period. Results showed that, among contact fungicides, thiram proved to be the least persistent while captan and fluazinam gave the best performance. Among the translaminar fungicides, SDHI protected the plants up to 7 days after the application
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Selettività di emamectina benzoato (Affirm®) nei confronti degli acari fitoseidi su vite e melo
No full textVariations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Valutazione dell’efficacia del nuovo formulato a base di emamectina benzoato (AFFIRM® OPTI) nei confronti di carpocapsa (Cydia pomonella) su pomacee
No full textAffirm® Opti è il nuovo formulato a base di emamectina benzoato in granuli idrodispersibili
(WG) sviluppato da Syngenta. Si caratterizza per la tecnologia “Pepite”, in grado di assicurare
una maggior stabilità della sostanza attiva, garantendo un’ottima efficacia a un dosaggio
inferiore rispetto all’attuale prodotto Affirm® in formulazione SG. Nel seguente lavoro si
riportano i risultati di alcune prove parcellari di campo condotte nel biennio 2014-2015 in
diversi areali italiani su melo e pero, per la lotta al lepidottero, Cydia pomonella. Oltre
all’efficacia su carpocapsa, a confronto con l’attuale formulato SG, le prove hanno verificato
la selettività verso le colture e la possibilità di inserimento della nuova formulazione WG in
una strategia adattata alle esigenze locali. Su tutte le colture le prove hanno dimostrato che il
prodotto, alla dose di 2 kg/ha di formulato commerciale, ha fornito risultati analoghi o
superiori alla formulazione SG utilizzata a 4 kg/ha e a vari prodotti standard di riferimento
(spinosad, clorpirifos e chlorantraniliprole), con una selettività apparsa ottimal
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