1,721,092 research outputs found
La richesse référentielle dans le corpus
No full textCohen-Scali M., Marcellesi Christiane. La richesse référentielle dans le corpus. In: Langue française, n°32, 1976. Aspects socio-culturels de l'enseignement du Français, sous la direction de Christiane Marcellesi. pp. 29-39
L'osservazione del processo penale minorile. Proposta di una griglia di analisi comunicativa
No full textGrapevine origin and diversity
No full textThis text is intended to be a brief discussion of some salient conceptual and methodological aspects concerning the cultivated grapevine (Vitis vinifera L). The essay, far from aiming to be exhaustive, strives instead to highlight original and significant steps taken by the international scientific community over the last thirty years to deepen the historical and biological path that this woody species, which has played an essential role in human history, has taken. Possible future scenarios involving genetic modifications to improve adaptive mechanisms are also discussed
Pollen tube taxol dependent structures co-assemble with neuronal HMW MAPs (MAP2)
No full textPollen tube microtubules (MTs) are as dynamic as animal MTs and they may interact with plasma membrane, endoplasmic reticulum, Golgi apparatus, mitocondria and a variety of cytoplasmic proteins. Bridges connecting MTs to each other and to membranes have been documented in pollen tubes by electron microscopy; however, the biochemical and molecular nature of these linkages is not known. In other cell types interaction between organelles and MTs require the participation of Microtubule-Associated Proteins (MAPs) that bridge the cytoskeleton to these organelles. Although biochemical documentation of such bridging MAPs in plant cells is lacking, it is reasonable to assume, by analogy with the animal systems, that specialized MAPs regulate MTs polymerization and dynamic in pollen tube. As a first step toward testing this hypothesis, the ability of Nicotiana tabacum pollen tube taxol-stabilized MTs to bind mammalian brain High Molecular Weight MAPs (HMWMAPs)(MAP2) was tested. This association analysis revealed the presence of mammalian MAP2-binding sites on pollen tube taxol-induced structures suggesting that the association presumably occurs at conserved domains on the tubulin molecules
Molecular markers and genomics for food and beverages characterization
No full textWe summarize and discuss latest trends and works on methods and most significant case studies concerning molecular-based food and beverages quality and authenticity assessment. DNA-based technology may work as a wild card between food authentication, gut microbiome profiling, and the evaluation of food-borne microbiological risk. The central axiom of food security necessarily passes through the validation of the concept of food authenticity that in turn depends on the analytical assessment of ingredients. With increasing molecular detail, literature demonstrates how the food intake is associated with specific epigenetic effects on genomes leading to gene expression regulation. Similarly, food intake deeply affects human health by influencing secondary metabolite production regulated by the gut microbiome. In the last decade, biology moved rapidly from small numbers to big data, keeping the DNA as a major tagged molecule for food characterization. Simple molecular markers have the advantage to be used by small-medium laboratories and may be effective in addressing simple questions, while the integrated genomics approaches address exhaustive description of food and beverages quality traits. More significant case studies of molecular characterization of plant and animal-derived food and grapevine- derived beverages are also reported. Finally we conclude on how the interdisciplinary and multidisciplinary approaches could improve our relation with food and beverages from the point of view of health and quality of life and against frauds. Visual Abstract DNA-based methods integrated with bioinformatics for genetic identification may act as a wild card under several molecular approaches that intercept human health issues. Food has a deep impact on human health on a direct basis through its nutritional properties, its sanitary status, affecting the gut microbiome and the cell epigenome, and likely, under certain circumstances, a stochastic mechanism even by transforming the cell genome itself. DNA-based authentication can be effective in profiling food microbiome related to the potential risk of causing food-borne diseases and food intrinsic qualities related to raw ingredients and origin. The DNA test aimed to address food authentication should be planned according to the specific goal and type of food or beverage, where species identification or intraspecific identification are selectively required
Grapevine (Vitis vinifera L.) Germplasm from Siena (Italy) Includes Rare Strains and Reveals Population Structuring
Full text linkMolecular characterization was carried out on samples of historical grapevine populations that were gathered from within and around the medieval walls of Siena. Forty-nine grapevines were selected based on their age, historical site of growth, grapevines’ ampelography, and for being relict accessions, obsolete to cultivation. SSR profiling data were compared to 44 known grapevines, revealing six functional genetic groups with significant similarity to grapevine types generally grown in Tuscany. The Sienese germplasm is enriched with rare grapevines at risk of extinction, such as Zuccaccio, Gorgottesco, Tenerone, Prugnolo gentile, Occhio di Pernice, Procanico, Rossone, Mammolo, and Canina. Population genetics analysis revealed the existence of five subpopulations structure (-k5) in analogy with cluster analysis. (Figure presented.)
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
A universal and rapid protocol for protein extraction from recalcitrant plant tissues for proteomic analysis
No full textA simple and universally applicable protocol for extracting high-quality proteins from recalcitrant plant tissues is described. We have used the protocol with no modification, for a wide range of leaves and fruits. In all cases, this protocol allows to obtain good electrophoretic separation of proteins. As the protocol is rapid, universal, and compatible with silver staining, it could be used for routine protein extraction from recalcitrant plant tissues for proteomic analysi
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