38,779 research outputs found
Research of changes in the level of vascular endothelial growth factor in experimental diabetic retinopathy. comparison of methods of correction
Get PDFSirman Ya. V., Savytskyi I. V. Research of changes in the level of vascular endothelial growth factor in experimental diabetic retinopathy. comparison of methods of correction. Journal of Education, Health and Sport. 2021;11(04): 247-257. eISSN 2391-8306. https://apcz.umk.pl/JEHS/article/view/40287
https://zenodo.org/record/7115445
The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019.
© The Authors 2021;
This article is published with open access at Licensee Open Journal Systems of Nicolaus Copernicus University in Torun, Poland
Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike. (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.
The authors declare that there is no conflict of interests regarding the publication of this paper.
Received: 26.02.2021. Revised: 31.03.2021. Accepted: 30.04.2021.
UDC:616-06:616-092.9
Research of changes in the level of vascular endothelial growth factor in experimental diabetic retinopathy. comparison of methods of correction
Ya. V. Sirman, I. V. Savytskyi
GE «Ukrainian Research Institute of Transport Medicine of the Ministry of Health of Ukraine»
Correspondence author: Savytskyi Ivan Volodymyrovich, 65039, Odesa, Fountain road 4-а/29, tel.+38050-381-21-83, [email protected]
Abstract
The aim of work to investigate the changes in the vascular endothelium growth factor in experimental animals, which were simulated diabetic retinopathy and against the background of its correction.
The results of the analysis of the vascular growth factor in the 2nd group confirm the development of diabetic retinopathy, a particularly pronounced increase in the marker was detected on the 180th day of the development of the pathological process. It was established analyzing the data of group No. 3 that the correction of the pathological condition with hypoglycemic agents has partial positive effect but requires the involvement of additional means of correction in addition to the normalization of the level of glycemia. The results of the 4th group indicate that the involvement of a nitric oxide donor and aflibercept in the correction of diabetic retinopathy has positive effect on the reduction of the level of vascular growth factor, more pronounced compared to the 3rd group, but it does not reach normative values. It is observed that the correction of the simulated pathological condition by reducing hyperglycemia, administration of aflibercept and bromfenac (group No. 5) gives positive results, but less pronounced, compared to the data of the 4th group.
It was found that in rats in which diabetic retinopathy was modeled with subsequent correction of hyperglycemia, administration of aflibercept, L-carnitine and bromfenac (group No. 6) at the first stage, there is a pronounced effectiveness of the proposed method of correction in comparison with the previously considered methods, the established positive trend is being followed also at the 2nd and 3rd stages of the experiment, which indicates the effectiveness of this correction method.
The most pronounced positive effect of normalizing the level of vascular growth factor is observed when using a hypoglycemic drug in combination with the administration of aflibercept, a solution of L-arginine and citicoline (in group No. 7).
Keywords: experimental diabetic retinopathy; streptozotocin diabetes; vascular growth factor; correction; metformin; aflibercept; bromfenac; L-carnitine; L-arginine; citicoline
Metabolic syndrome: review
Get PDFBobrova M. S., Vorona S. O., Koval S. Yu., Savytskyi I. V., Merza Y. M., Savytskyi V. I. Metabolic syndrome: review. Journal of Education, Health and Sport. 2021;11(07):274-287. eISSN 2391-8306. DOI http://dx.doi.org/10.12775/JEHS.2021.11.07.026
https://apcz.umk.pl/JEHS/article/view/JEHS.2021.11.07.026
https://zenodo.org/record/5542161
The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019.
© The Authors 2021;
This article is published with open access at Licensee Open Journal Systems of Nicolaus Copernicus University in Torun, Poland
Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,
provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike.
(http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.
The authors declare that there is no conflict of interests regarding the publication of this paper.
Received: 25.06.2021. Revised: 12.07.2021. Accepted: 29.07.2021.
METABOLIC SYNDROME: REVIEW
M. S. Bobrova1, S. O. Vorona2, S. Yu. Koval3, I. V. Savytskyi3,
Y. M. Merza3, V. I. Savytskyi4
1Volodymyr Vynnychenko Central Ukrainian State Pedagogical University, Kropyvnytskyi
2Kirovohrad Scientific Research Forensic Center of Ministry of Internal Affairs of Ukraine, Kropyvnytskyi
3International European University, Kyiv
4Odesa National Medical University, Odesa
Abstracts
The psychosomatic component of the metabolic syndrome, historical aspect of the problem under study, physiological and biochemical mechanisms of pathogenesis are considered in the article. The symptomatic components of the metabolic syndrome and their interrelation are outlined, the ways of diagnosis and therapy and directions of modern research in this field are outlined.
Key words: metabolic syndrome; insulin resistance; hypertonic disease; abdominal obesity
Dynamic of blood glucose levels in the model of experimental diabetic retinopathy and different ways to correct it
Get PDFSirman Y. V., Savytskyi I. V., Blavatska O. M. Dynamic of blood glucose levels in the model of experimental diabetic retinopathy and different ways to correct it. Journal of Education, Health and Sport. 2021;11(04): 366-373. eISSN 2391-8306. DOI http://dx.doi.org/10.12775/JEHS.2021.11.07.036
https://apcz.umk.pl/czasopisma/index.php/JEHS/article/view/JEHS.2021.11.07.036
https://zenodo.org/record/7115363
The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019.
© The Authors 2021;
This article is published with open access at Licensee Open Journal Systems of Nicolaus Copernicus University in Torun, Poland
Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike. (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.
The authors declare that there is no conflict of interests regarding the publication of this paper.
Received: 06.07.2021. Revised: 11.07.2021. Accepted: 29.07.2021.
UDC:616-06:616-092.9
Dynamic of blood glucose levels in the model of experimental diabetic retinopathy and different ways to correct it
Y. V. Sirman, I. V. Savytskyi, O. M. Blavatska
GE «Ukrainian Research Institute of Transport Medicine of the Ministry of Health of Ukraine»
Correspondence author: Savytskyi Ivan Volodymyrovich, 65039, Odesa, Fountain road 4-а/29, tel.+38050-381-21-83, [email protected]
Abstract
The aim of our study was to analyze the changes in the blood glucose level in experimental animals that simulated diabetic retinopathy and against the background of its correction. The obtained results show the development of experimental diabetes when streptozotocin is administered at a dose of 55 mg/kg of animal weight against the background of a high-fat diet. Correction with hypoglycemic agents in the 3rd group had a positive effect, but it was not able to completely normalize the level of glycemia, so the need arose for the use of additional agents. The use of aflibercept and a nitric oxide donor in the 4th group had a more pronounced positive effect compared to the 3rd group, but the result did not reach the control indicators. Combined administration of bromfenac and aflibercept in the 5th group showed itself to be less effective in the study of the blood glucose level at all stages in comparison with the data of the 4th group, in which the NO donor was involved in the correction. Administation of aflibercept, L-carnitine and bromfenac to the animals of the 6th group had a more pronounced positive effect in comparison with the group No. 5, but did not reach the values of the 4th group of the experiment. The most effective correction was the combination of metformin, aflibercept, L-arginine and citicoline in rats of the 7th group, which is evidenced by the normalization of the level of the studied indicators on the 30th and 60th days of the experiment, and on the 180th, the most pronounced decrease was recorded hyperglycemia.
Key words: experimental diabetic retinopathy; streptozotocin diabetes; blood glucose; correction; metformin; aflibercept; bromfenac; L-carnitine; L-arginine; citicoline
Results of histological examination of experimental diabetic retinopathy and its correction with metformin, aflibercept, L-arginine solution and cyticolin
Get PDFSirman Y. V., Savytskyi I. V., Preys N. I., Blavatska O. M. Results of histological examination of experimental diabetic retinopathy and its correction with metformin, aflibercept, L-arginine solution and cyticolin. Journal of Education, Health and Sport. 2020;10(12):426-435. eISSN 2391-8306. DOI http://dx.doi.org/10.12775/JEHS.2020.10.12.042
https://apcz.umk.pl/czasopisma/index.php/JEHS/article/view/JEHS.2020.10.12.042
https://zenodo.org/record/7112906
The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019.
© The Authors 2020;
This article is published with open access at Licensee Open Journal Systems of Nicolaus Copernicus University in Torun, Poland
Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,
provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike.
(http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.
The authors declare that there is no conflict of interests regarding the publication of this paper.
Received: 09.11.2020. Revised: 25.11.2020. Accepted: 29.12.2020.
UDC:616-06:616-092.9
Results of histological examination of experimental diabetic retinopathy and its correction with metformin, aflibercept, L-arginine solution and cyticolin
Y. V. Sirman, I. V. Savytskyi, N. I. Preys, O. M. Blavatska
GE «Ukrainian Research Institute of Transport Medicine of the Ministry of Health of Ukraine»
Correspondence author: Savytskyi Ivan Volodymyrovich, 65039, Odesa, Fountain road 4-а/29, tel.+38050-381-21-83, [email protected]
Abstract
The purpose of the study was to analyze the histological changes in the structures of the eyeball in experimental diabetic retinopathy and its correction with metformin, aflibercept, L-arginine solution and citicoline.
The obtained results indicate about the development of pathological changes in the retina against the background of the progression of experimental diabetes when streptozotocin is administered at dose 55 mg/kg of animal weight against the background of a high-fat diet. The development of simulated streptozotocin diabetes is accompanied by disturbance in the retina in the form of edematous shifts of fibers and swelling of the cells forming it, uneven distribution of cells of the ganglion layer and vacuolization of the cytoplasm some of them. The using of corrective drugs complex for metabolic disorders and angiopathy, which are characteristic of diabetes mellitus, affects on the retina condition. Under the influence of aflibercept, solution of L-arginine, citicoline, and metformin administration was established that edematous changes in its structural elements are significantly reduced, the number of vessels on the border with the retina visually increases, and the staining of the cells of the ganglion layer becomes more freshly.
Key words: experimental diabetic retinopathy; streptozotocin diabetes; histological examination; correction; metformin; aflibercept; L-arginine; citicoline
The choice of the optimal experimental model of myocardial infarction
Get PDFKoval'skaja V. V., Savytskyi I. V., Mironov A. А. The choice of the optimal experimental model of myocardial infarction. Journal of Education, Health and Sport. 2017;7(4):796-801. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.801185
http://ojs.ukw.edu.pl/index.php/johs/article/view/4494
The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 1223 (26.01.2017).
1223 Journal of Education, Health and Sport eISSN 2391-8306 7
© The Author (s) 2017;
This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland
Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,
provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License
(http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.
This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial
use, distribution and reproduction in any medium, provided the work is properly cited.
The authors declare that there is no conflict of interests regarding the publication of this paper.
Received: 12.04.2017. Revised 10.04.2017. Accepted: 25.04.2017.
THE CHOICE OF THE OPTIMAL EXPERIMENTAL MODEL OF MYOCARDIAL INFARCTION
V. V. Koval'skaja, I. V. Savytskyi, A. А. Mironov
Odessa National Medical University, Odessa
Abstract
Nowadays myocardial infarction has a high percentage of mortality [12], and is widespread in Ukraine and Western countries [1, 2, 3]. Such a situation calls for the development of a new model of myocardial infarction, which would be as simple and effective as possible. In this article, a model of myocardial infarction with the use of inhalation anesthesia with chloroform is proposed.
Key words: myocardial infarction, experiment, experimental model, rat, chloroform, adrenaline
Research of changes in the level of endothelin-1 in experimental diabetic retinopathy. comparison of methods of correction
Get PDFSirman Y. V., Preis N. I., Savytskyi I. V. Research of changes in the level of endothelin-1 in experimental diabetic retinopathy. comparison of methods of correction. Journal of Education, Health and Sport. 2021;11(10):411-422. eISSN 2391-8306. DOI http://dx.doi.org/10.12775/JEHS.2021.11.10.038
https://apcz.umk.pl/czasopisma/index.php/JEHS/article/view/JEHS.2021.11.10.038
https://zenodo.org/record/6449122
The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019.
© The Authors 2021;
This article is published with open access at Licensee Open Journal Systems of Nicolaus Copernicus University in Torun, Poland
Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,
provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike.
(http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.
The authors declare that there is no conflict of interests regarding the publication of this paper.
Received: 30.09.2021. Revised: 12.10.2021. Accepted: 29.10.2021.
UDC:616-06:616-092.9
RESEARCH OF CHANGES IN THE LEVEL of ENDOTHELIN-1 IN EXPERIMENTAL DIABETIC RETINOPATHY. COMPARISON OF METHODS OF CORRECTION
Y. V. Sirman, N. I. Preis, I. V. Savytskyi
SE "Ukrainian Research Institute of Transport Medicine of the Ministry of Health of Ukraine"
Abstract
Diabetic retinopathy (DR) according to the WHO is the main cause of decreased vision and blindness due to diabetes.
The aim of the study: to compare the effectiveness of correction of vasoconstriction in experimental diabetic retinopathy under the influence of different methods of correction.
Our results indicate a violation of the functional state of blood vessels on the 30th day of experimental diabetic retinopathy with subsequent progression of pathological changes on the 60th and 180th day of the study, as evidenced by a significant increase in endothelin-1 in the 2nd group (p <0.001), most pronounced in the 3rd stage.In the analysis of data of group №3 it was found that the correction of pathological conditions with hypoglycemic agents has a positive effect, but does not allow to significantly correct pathologically increased levels of endothelin-1, which indicates the need for additional correction tools other than hyperglycemia.The results of group 4 indicate that the involvement of a donor of nitric oxide and aflibercept in the correction of diabetic retinopathy corrects the level of the studied marker, the most pronounced effect is observed on the 180th day of the experiment, but normative values can not be achieved. It was found that in the 5th group, in which the correction of the simulated pathological condition was performed by reducing hyperglycemia, the introduction of aflibercept and bromfenac gives positive results, but less pronounced than in the 4th group, where L-arginine solution was added to the complex correction. .It was found that rats of group 6, which simulated diabetic retinopathy with subsequent correction of hyperglycemia, administration of aflibercept, L-carnitine and bromfenac have a pronounced effectiveness of the proposed method of correction compared to previous methods, the level of vasoconstriction stage and continues to recover in the following stages of the experiment, but does not reach the normative values. The obtained data suggest that the method of correction selected in group 7, which includes reduction of hyperglycemia, administration of aflibercept, solution of L-arginine and citicoline is most effective for normalizing vascular tone and structural and functional state of the endothelium.
Key words: experimental diabetic retinopathy; endothelial dysfunction; endothelin-1; vasoconstriction; correction
DYNAMICS OF BIOCHEMICAL MARKERS IN THE PATHOGENESIS OF EXPERIMENTAL BILE PERTONITIS
No full textZnamerovsky S. G., Savytskyi I. V., Dzygal O. F. DYNAMICS OF BIOCHEMICAL MARKERS IN THE PATHOGENESIS OF EXPERIMENTAL BILE PERTONITIS. ВІСНИК МОРСЬКОЇ МЕДИЦИНИ. 2020;3(88): 84-90. ISSN 0049-6804.
DOI http://dx.doi.org/10.5281/zenodo.4156227
http://www.herald.com.ua
УДК 616 - 092.9 : 616 - 0.35
DOI http://dx.doi.org/10.5281/zenodo.4156227
DYNAMICS OF BIOCHEMICAL MARKERS IN THE PATHOGENESIS OF
EXPERIMENTAL BILE PERTONITIS
S. G. Znamerovsky, I. V. Savytskyi, O. F. Dzygal
Odessa National Medical University, Odessa, Ukraine
Summary
Biliary peritonitis (BP), which is a severe disease of the abdominal cavity in severity, prognosis and mortality rate depends on endogenous intoxication Systemic Inflammatory Reaction Syndrome (SIRS) is considered in surgical practice as a non-specific response to injury. The study was performed on 180 Wistar rats weighing 180-200 g. The animals were divided into 4 groups. As a result of the conducted researches it is proved that the level of leukocytes is an important diagnostic test in the pathogenesis of experimental peritonitis. When studying the dynamics of leukocyte levels on the first and third days of the experiment, its increase is noted against the background of experimental biliary peritonitis. The results of the 4th group indicate a decrease in this indicator against the background of the proposed method of remediation and are closer to the values of the norm in comparison with the 3rd group. On the 7th day of the experiment, data on the normalization of this marker in the 4th group were obtained.
Key words: biliary peritonitis, model, sanitation of abdominal cavity, leukocytes, correctio
Study of indicators of endothelial dysfunction in rats with experimental peritonitis
Get PDFGutsulyuk V. G., Savytskyi I. V., Tsypoviaz S. V., Znamerovskyi S. G., Sarahan V. M., Gerasymenko T. V. Study of indicators of endothelial dysfunction in rats with experimental peritonitis. Journal of Education, Health and Sport. 2022;12(1):514-522. eISSN 2391-8306. DOI http://dx.doi.org/10.12775/JEHS.2022.12.01.043
https://apcz.umk.pl/JEHS/article/view/41740
https://zenodo.org/record/7512638
The journal has had 40 points in Ministry of Education and Science of Poland parametric evaluation. Annex to the announcement of the Minister of Education and Science of December 1, 2021. No. 32343.
Has a Journal's Unique Identifier: 201159. Scientific disciplines assigned: Physical Culture Sciences (Field of Medical sciences and health sciences); Health Sciences (Field of Medical Sciences and Health Sciences).
Punkty Ministerialne z 2019 - aktualny rok 40 punktów. Załącznik do komunikatu Ministra Edukacji i Nauki z dnia 1 grudnia 2021 r. Lp. 32343. Posiada Unikatowy Identyfikator Czasopisma: 201159.
Przypisane dyscypliny naukowe:Nauki o kulturze fizycznej (Dziedzina nauk medycznych i nauk o zdrowiu); Nauki o zdrowiu (Dziedzina nauk medycznych i nauk o zdrowiu).
© The Authors 2022;
This article is published with open access at Licensee Open Journal Systems of Nicolaus Copernicus University in Torun, Poland
Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,
provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike. (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.
The authors declare that there is no conflict of interests regarding the publication of this paper.
Received: 05.01.2022. Revised: 21.01.2022. Accepted: 31.01.2022.
UDC 616.381-018.74
Study of indicators of endothelial dysfunction in rats with experimental peritonitis
V. G. Gutsulyuk, I. V. Savytskyi, S. V. Tsypoviaz, S. G. Znamerovskyi, V. M. Sarahan, T. V. Gerasymenko
International Academy of Ecology and Medicine, Kyiv, Ukraine
Correspondence author: Savytskyi Ivan Volodymyrovich, 65039, Odesa, Fountain road4-а/29, tel.+38050-381-21-83, [email protected]
Abstract
The article presents the results of the study of the activity of endothelial and inducible NO-synthase, the level of Willebrand factor and endothelin-1 in rats with experimental peritonitis. The most likely mechanism that is damaged in the endothelium during peritonitis is the activation of the synthesis of inducible NO-synthase by neutrophils/macrophages in response to infection. It is possible that hyperproduction of nitric oxide (NO), on the one hand, is aimed at destroying microflora and oxidizing toxins, and on the other hand, at suppressing the expression of tissue factor and cell adhesion molecules. platelet aggregation and cascade disorders in the hemostasis system. All this indicates that the hyperproduction of NO not only reflects the processes that occur in the focus of damage to the vascular endothelium, but also affects the severity of the inflammatory process and the outcome of the disease. In animals with experimental peritonitis on the background of OS, an increase in the number of circulating desquamated endothelial cells in the blood, which is a highly specific marker of endothelial dysfunction, was noted. The level of the Willebrand factor also increased, which can serve as a marker of increased risk of thrombus formation and indicate the pathogenetic dependence of the factors that damage the vascular wall endothelium on the concentration of the Willebrand factor, which contributes to the reduction of vascular permeability by adhesion of platelets to the endothelium. Confirmation of the development of endothelial dysfunction in peritonitis is an increase in the concentration of endothelin-1, which is a regulator of the process of vascular neoangiogenesis in response to endothelial damage.
Keywords: peritonitis; endothelial dysfunction; oxidative stress; Willebrand factor; pathogenesis
Study of the role of erythrocyte and leukocyte intoxication indexes in the pathogenesis of experimental peritonitis
No full textGutsulyuk V. G., Savytskyi I. V., Tsypoviaz S. V., Znamerovskyi S. G., Zashchuk R. G. Study of the role of erythrocyte and leukocyte intoxication indexes in the pathogenesis of experimental peritonitis. Journal of Education, Health and Sport. 2022;12(4):341-346. eISSN 2391-8306. DOI http://dx.doi.org/10.12775/JEHS.2022.12.04.029
https://apcz.umk.pl/JEHS/article/view/41779
https://zenodo.org/record/7514133
The journal has had 40 points in Ministry of Education and Science of Poland parametric evaluation. Annex to the announcement of the Minister of Education and Science of December 1, 2021. No. 32343.
Has a Journal's Unique Identifier: 201159. Scientific disciplines assigned: Physical Culture Sciences (Field of Medical sciences and health sciences); Health Sciences (Field of Medical Sciences and Health Sciences).
Punkty Ministerialne z 2019 - aktualny rok 40 punktów. Załącznik do komunikatu Ministra Edukacji i Nauki z dnia 1 grudnia 2021 r. Lp. 32343. Posiada Unikatowy Identyfikator Czasopisma: 201159.
Przypisane dyscypliny naukowe:Nauki o kulturze fizycznej (Dziedzina nauk medycznych i nauk o zdrowiu); Nauki o zdrowiu (Dziedzina nauk medycznych i nauk o zdrowiu).
© The Authors 2022;
This article is published with open access at Licensee Open Journal Systems of Nicolaus Copernicus University in Torun, Poland
Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,
provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike. (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.
The authors declare that there is no conflict of interests regarding the publication of this paper.
Received: 23.03.2022. Revised: 04.04.2022. Accepted: 29.04.2022.
UDC 616-092:616-036.8
Study of the role of erythrocyte and leukocyte intoxication indexes in the pathogenesis of experimental peritonitis
V. G. Gutsulyuk, I. V. Savytskyi, S. V. Tsypoviaz, S. G. Znamerovskyi, R. G. Zashchuk
International Academy of Ecology and Medicine, Kyiv, Ukraine
Correspondence author: Savytskyi Ivan Volodymyrovich, 65039, Odesa, Fountain road4-а/29, tel.+38050-381-21-83, [email protected]
Abstract
The article presents the results of studying the role of erythrocyte and leukocyte indices of intoxication in the pathogenesis of experimental peritonitis. When studying the role of erythrocyte and leukocyte indices, a unidirectional trend of their change was revealed. An increase in the erythrocyte index of intoxication in the pathogenesis of experimental fecal peritonitis was established, most pronounced on the 10th day of the experiment (the level of EII increased by 107% compared to intact animals). The results of the study of the leukocyte intoxication index indicate a unidirectional trend, as in the case of the erythrocyte intoxication index. On the first day, the leukocyte index of intoxication increased on 225% (р<0.05), on the 4th day – on 256.7% (р<0.05), and on the 10th day – on 260% (р <0.05) compared to intact animals. It has been proven that the leukocyte index of intoxication is a more sensitive marker compared to the erythrocyte index.
Keywords: peritonitis; leukocyte index of intoxication; erythrocyte index of intoxication; pathogenesis
Results of construction of correlation fields with trend lines and determination of direction of relationships between studied indicators
Get PDFSirman Y. V., Preys N. I., Savytskyi I. V. Results of construction of correlation fields with trend lines and determination of direction of relationships between studied indicators. Journal of Education, Health and Sport. 2021;11(06): 348-361. eISSN 2391-8306. https://apcz.umk.pl/JEHS/article/view/40290
https://zenodo.org/record/7115435
The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019.
© The Authors 2021;
This article is published with open access at Licensee Open Journal Systems of Nicolaus Copernicus University in Torun, Poland
Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike. (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.
The authors declare that there is no conflict of interests regarding the publication of this paper.
Received: 26.05.2021. Revised: 30.05.2021. Accepted: 30.06.2021.
UDC:616-06:616-092.9
Results of construction of correlation fields with trend lines and determination of direction of relationships between studied indicators
Y. V. Sirman, N. I. Preys, I. V. Savytskyi
GE «Ukrainian Research Institute of Transport Medicine of the Ministry of Health of Ukraine»
Correspondence author: Savytskyi Ivan Volodymyrovich, 65039, Odesa, Fountain road 4-а/29, tel.+38050-381-21-83, [email protected]
Abstract
The aim of work is construction of correlation fields with trend lines and determination of the directionality of relationships between the investigated indicators of endothelial dysfunction, hypoxia and oxidative stress.
Our results indicate that Willebrand factor, endothelin-1, 2,3-diphosphoglycerate, MDA, diene conjugates and eNOS are informative markers in the pathogenesis of experimental diabetic retinopathy. As a result of the research, it was established that there is a direct linear relationship between the Willebrand factor indicator and malondialdehyde, since the points of the correlation field are practically located on a straight line. We trace a similar type of direct linear relationship between Willebrand factor and diene conjugates. The specified results confirm the mutually aggravating relationship between the development of endothelial dysfunction and oxidative stress. We trace an inverse linear relationship between Willebrand factor and peroxidase. With an increase in Willebrand factor indicators by stages, we trace a decrease in peroxidase indicators, which indicates a relationship between endothelial dysfunction and a weakening of antioxidant protection. As a result of the study, it was established that the first two indicators: malondialdehyde and diene conjugates have an almost direct linear relationship with endothelin-1 indicators. These results confirm the relationship between the functional and structural state of the endothelium and the development of oxidative stress. It was established that the peroxidase indicator has an inverse linear relationship with the endothelin-1 indicators, which, as in the case of the Willebrand factor, indicates a violation of antioxidant protection in the event of endothelial dysfunction. As a result of the analysis of the dependence of the indicators of 2,3 diphosphoglycerate, Willebrand factor and eNOS, it was found that in the first two pairs of indicators, there is an inverse linear relationship between the indicators. As eNOS levels increase by stage, levels of 2,3 diphosphoglycerate and Willebrand factor decrease. In the case of an inverse relationship between the parameters of the Willebrand factor and eNOS, a practically functional connection is observed, since the coefficient of determination is almost 1. The obtained results indicate that the violation of the structural and functional state of the endothelium and the development of hypoxia are interconnected with the violation of the physiological pathway of oxide synthesis nitrogen. The relationship between the indicators of 2,3 diphosphoglycerate and the Willebrand factor was revealed, which has a direct relationship between the variables, which once again confirms the aggravating interaction between hypoxia and endothelial dysfunction.
Keywords: experimental diabetic retinopathy; streptozotocin diabetes; endothelial dysfunction; oxidative stress; hypoxia; nitric oxide synthesis
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