266 research outputs found
Effect of pre-<i>in vitro</i> maturation with cAMP modulators on the acquisition of oocyte developmental competence in cattle
No full textThe administration of follicle-stimulating hormone (FSH) prior to oocyte retrieval improves oocyte developmental competence. During bovine embryo production in vitro, however, oocytes are typically derived from FSH-unprimed animals. In the current study, we examined the effect of pre-in vitro maturation (IVM) with cAMP modulators, also known as the second messengers of FSH, on the developmental competence of oocytes derived from small antral follicles (2–4 mm) of FSH-unprimed animals. Pre-IVM with N6,2ʹ-O-dibutyryladenosine 3′,5′-cyclicmonophosphate (dbcAMP) and 3-isobutyl-1-methylxanthine (IBMX) for 2 h improved the blastocyst formation in oocytes stimulated by FSH or amphiregulin (AREG). Furthermore, pre-IVM enhanced the expression of the FSH-or AREG-stimulated extracellular matrix-related genes HAS2, TNFAIP6, and PTGS2, and epidermal growth factor (EGF)-like peptide-related genes AREG and EREG. Additionally, pre-IVM with dbcAMP and IBMX enhanced the expression of EGFR, and also increased and prolonged cumulus cell-oocyte gap junctional communication. The improved oocyte development observed using the pre-IVM protocol was ablated by an EGF receptor phosphorylation inhibitor. These results indicate that pre-IVM with cAMP modulators could contribute to the acquisition of developmental competence by bovine oocytes from small antral follicles through the modulation of EGF receptor signaling and oocyte-cumulus/cumulus-cumulus gap junctional communication. © 2018 by the Society for Reproduction and Development
DEVELOPMENT OF PRE-IMPLANTATION EMBRYO ASSESSMENT TECHNIQUES TO CREATE OPTIMAL CULTURE ENVIRONMENTS
No full text近畿大学Kindai University博士（工学）主査：山縣 一夫 教授
学内授与番号：生第66号Tatsuma Yao, Rie Suzuki, Natsuki Furuta, Yuka Suzuki, Kyoko Kabe, Mikiko Tokoro, Atsushi Sugawara, Akira Yajima, Tomohiro Nagasawa, Satoko Matoba, Kazuo Yamagata, Satoshi Sugimura
"Live-cell imaging of nuclear–chromosomal dynamics in bovine in vitro fertilised embryos. " Scientific Reports 2018, 8 (1), 7460
Tatsuma Yao, Akane Ueda, Atchalalt Khurchabilig, Daisuke Mashiko, Mikiko Tokoro, Hiroki Nagai, Tei Sho, Satoko Matoba, Kazuo Yamagata, Satoshi Sugimura "Micronucleus formation during early cleavage division is a potential hallmark of preimplantation embryonic loss in cattle." Biochemical and Biophysical Research Communications, 617(2022), Pages 25-32
https://doi.org/10.1016/j.bbrc.2022.05.075 掲載doctoral thesi
Trajectories of victimization across the school years: individual and peer context predictors
No full textPeer victimization can be a chronic stressor for some youth but not all youth. To understand the predictors of continuity and discontinuity of overt and relational victimization over an extended period of time, this study examined the independent and unique contributions of predictors that are associated with individual characteristics (social behavior) and a contextual factor (peer adversity), from 2nd to 8th grade, spanning the transition to middle school. In Chapter 1, the overall trajectories of victimization were found to decline for both types of victimization for both girls and boys. In Chapter 2, the independent contribution of each predictor to the intercepts and trajectories of victimization was examined. The analyses demonstrated that both individual and peer context factors contribute to 2nd grade victimization and the trajectories of victimization. Interestingly, some of these effects were stronger in boys during elementary school and in girls after the transition to middle school. In Chapter 3, unique contributions of predictors were investigated by examining the effects of predictors simultaneously. Overall, the results suggested that peer adversity did not predict 2nd grade victimization or the trajectories of victimization above and beyond individual characteristics. Results are discussed in regards to prevention of prolonged victimization, with attention to sex differences and youth who have multiple risk factors.Submission published under a 24 month embargo labeled 'U of I only', the embargo will last until 2017-08-01The student, Niwako Sugimura, accepted the attached license on 2015-07-01 at 14:50.The student, Niwako Sugimura, submitted this Dissertation for approval on 2015-07-01 at 15:00.This Dissertation was approved for publication on 2015-07-02 at 14:00.DSpace SAF Submission Ingestion Package generated from Vireo submission #8326 on 2015-09-29 at 14:58:55Made available in DSpace on 2015-09-29T20:49:39Z (GMT). No. of bitstreams: 2
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Previous issue date: 2015-07-02Embargo set by: Seth Robbins for item 89433
Lift date: 2017-09-29T20:50:34Z
Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD systemU of I Only Restriction Lifted for Item 89433 on 2017-09-30T09:15:30Z
Promotion of EGF receptor signaling improves the quality of low developmental competence oocytes
No full textAvailable online 14 May 2015Abstract not availableSatoshi Sugimura, Lesley J. Ritter, Ryan D. Rose, Jeremy G. Thompson, Johan Smitz, David G. Mottershead, Robert B. Gilchris
Oocyte induction of EGF responsiveness in somatic cells is associated with the acquisition of porcine oocyte developmental competence
No full textOocytes progressively acquire the competence to support embryo development as oogenesis proceeds with ovarian folliculogenesis. The objectives of this study were to investigate oocyte-secreted factor (OSF) participation in the development of somatic cell epidermal growth factor (EGF) responsiveness associated with oocyte developmental competence. A well-established porcine model was employed using oocytes from small (4 mm) antral follicles, representing low vs moderate developmental competence, respectively. Cumulus-oocyte complexes (COCs) were treated in vitro with inducers of oocyte maturation, and cumulus cell functions and oocyte developmental competence were assessed. COCs from small follicles responded to FSH but, unlike COCs from larger follicles, were incapable of responding to EGF family growth factors known to mediate oocyte maturation in vivo, exhibiting perturbed cumulus expansion and expression of associated transcripts (HAS2 and TNFAIP6). Low and moderate competence COCs expressed equivalent levels of EGF receptor (EGFR) mRNA; however, the former had less total EGFR protein leading to failed activation of phospho-EGFR and phospho-ERK1/2, despite equivalent total ERK1/2 protein levels. Native OSFs from moderate, but not from low, competence oocytes established EGF responsiveness in low competence COCs. Four candidate recombinant OSFs failed to mimic the actions of native OSFs in regulating cumulus expansion. Treatment with OSFs and EGF enhanced oocyte competence but only of the low competence COCs. These data suggest that developmental acquisition by the oocyte of capacity to regulate EGF responsiveness in the oocyte's somatic cells is a major milestone in the oocyte's developmental program and contributes to coordinated oocyte and somatic cell development.Lesley J. Ritter, Satoshi Sugimura, and Robert B. Gilchris
Oocyte maturation and quality: role of cyclic nucleotides
No full textAdvance Publication first posted online on 15 July 2016 - Accepted manuscriptThe cyclic nucleotides, cAMP and cGMP, are the key molecules controlling mammalian oocyte meiosis. Their roles in oocyte biology have been at the forefront of oocyte research for decades and many of the long standing controversies in relation to the regulation of oocyte meiotic maturation are now resolved. It is now clear that the follicle prevents meiotic resumption through the actions of natriuretic peptides and cGMP inhibiting the hydrolysis of intra-oocyte cAMP and that the preovulatory gonadotrophin surge reverses these processes. The gonadotrophin surge also leads to a transient spike in cAMP in the somatic compartment of the follicle; research over the past 2 decades has conclusively demonstrated that this surge in cAMP is important for the subsequent developmental capacity of the oocyte. This is important, as oocyte in vitro maturation (IVM) systems practiced clinically do not recapitulate this cAMP surge in vitro, possibly accounting for the lower efficiency of IVM compared to clinical IVF. This review focuses in particular on this latter aspect - the role of cAMP/cGMP in the regulation of oocyte quality. We conclude that clinical practice of IVM should reflect this new understanding of the role of cyclic nucleotides, thereby creating a new generation of ART and fertility treatment options.Gilchrist RB, Luciano AM, Richani D, Zeng HT, Wang X, De Vos M, Sugimura S, Smitz J, Richard FJ and Thompson J
Neuroendocrine differentiation in adenocarcinoma of prostate during combined androgen blockade therapy : a case report
No full text85歳男性.患者は3年半前に排尿困難で受診し, 前立腺生検でpoorly differentiated adenocarcinoma, T3bN0M0と診断された.Combined androgen blockade(CAB)療法で軽快したが, 今回, 再び排尿困難で受診となり, 直腸診で前立腺腫大を認めたため入院となった.検査では貧血と軽度の腎機能障害を認め, 腫瘍マーカーのPSAは感度以下で, CEAとCA19-9は正常値, NSEとPro-GRPが著明に上昇していた.またMRI T2強調像で内部不均一な前立腺, 精嚢の著明な腫大を認め, 更に直腸との境界は不明であったことから浸潤が疑われた.そして前回と全く異なり前立腺生検ではN/C比の高い, 高度異型核を有する未分化な細胞が充実性に存在し, 免疫染色ではPSA染色陰性, NSE, クロモグラニンA, グリメリウス染色陽性と逆の結果を示していた.NE(Neuroendocrine) carcinoma, T4N0M0と診断し, 無治療にて現在も生存中であるProstatic neuroendocrine (NE) carcinoma is a rare disease with a poor prognosis because of its rapid progression and the androgen-independent characteristic, for which no successful therapy is available presently. We report a case of NE differentiated prostate cancer, which was diagnosed as adenocarcinoma initially and progressed with NE differentiation during the combined androgen blockade therapy
Modifications of human growth differentiation factor 9 to improve the generation of embryos from low competence oocytes
No full textGrowth differentiation factor 9 (GDF9) is an oocyte-derived growth factor that plays a critical role in ovarian folliculogenesis and oocyte developmental competence, and belongs to the transforming growth factor-β (TGF-β) family of proteins. Recombinant human GDF9 (hGDF9) is secreted in a latent form, which in the case of the fully processed protein, has the pro-region non-covalently associated with the mature region. In this study we investigated a number of amino acid residues in the mature region of hGDF9 which are different from the corresponding residues in the mouse protein, which is not latent. We designed, expressed and purified four forms of chimeric hGDF9 (M1-M4) which we found to be active in a granulosa cell bioassay. Utilizing a porcine in vitro maturation (IVM) model with inherent low developmental competence (yielding 10-20 blastocysts) we tested the ability of the chimeric hGDF9 proteins to improve oocyte maturation and developmental competence. Interestingly, one of the chimeric proteins, M3, was able to significantly increase the level of embryo production utilizing such low competence oocytes. Our molecular modelling studies suggest that in the case of hGDF9 the Gly(391)Arg mutation likely increases receptor binding affinity thereby creating an active protein for granulosa cells in vitro. However, for an improvement in oocyte developmental competence a second mutation (Ser(412)Pro), which potentially decreases affinity of the mature region for the pro-region, is also required.Jing-Jie Li, Satoshi Sugimura, Thomas D. Mueller, Melissa A. White, Georgia A. Martin, Lesley J. Ritter, Xiao-Yan Liang, Robert B. Gilchrist, and David G. Mottershea
Interspecies Somatic Cell Nuclear Transfer Technique for Researching Dog Cloning and Embryonic Stem Cells
No full textA Cooled Vortex Street Generated by Cooling a Circular Cylinder in Low Reynolds Number Wake (1st Report, Change of Flow Pattern)
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