394 research outputs found
Internal Genomic DNA Standard for Quantitative Metagenome Analysis v3
No full textSatinsky, Brandon M., et al. 'Use of internal standards for quantitative metatranscriptome and metagenome analysis.' Methods in enzymology 531 (2012): 237-250. </p
Internal Genomic DNA Standard for Quantitative Metagenome Analysis v1
No full textSatinsky, Brandon M., et al. 'Use of internal standards for quantitative metatranscriptome and metagenome analysis.' Methods in enzymology 531 (2012): 237-250. </p
Preparation of Custom Synthesized RNA Transcript Standard v1
No full textThis protocol is from: Satinsky, Brandon M., et al. "Use of internal standards for quantitative metatranscriptome and metagenome analysis." Methods in enzymology 531 (2012): 237-250. Please see the full chapter for additional details. </p
Preparation of Custom Synthesized RNA Transcript Standard v3
No full textThis protocol is from: Satinsky, Brandon M., et al. "Use of internal standards for quantitative metatranscriptome and metagenome analysis." Methods in enzymology 531 (2012): 237-250. Please see the full chapter for additional details. </p
Exploring improvisation pedagogy based on participatory and M-base characteristics: an action research study
No full textThis action research study explored my personal pedagogy of improvisation through a combined lens of Thomas Turino’s (2008) participatory field of music and the M-base collective. Influenced by the imbalance between performance-based goals and presentational music-making in high school settings and my personal lack of experience teaching improvisation, this study focused on learning how to teach improvisation away from typical educational outcomes such as assessment, curricular outcomes, and presentational values. Four high school students and two post-high school musicians participated in four two-hour sessions which took place at the Bassment in Saskatoon, Saskatchewan. Through the use of an action research cycle, several key themes emerged based on the participants’ experiences in regards to the purpose, process and reason for teaching improvisation. I came to the following four conclusions based on the actions that occurred during the study: a teacher needs to create attainable levels for successful improvisation; the facilitator role requires time for reflection and practice; participating allowed me to recognize and challenge personal self-doubt around improvisation; and improvisation can thrive in a non-traditional environment through a balance between structure and freedom. The thesis concludes with future recommendations for educators, researchers, and myself.
Keywords: improvisation, participatory, M-base, secondary, music education, action researchIncludes bibliographical references (pages 133-147)."In partial fulfillment for the degree of Master of Music (Education), Brandon University
Jenni Brandon: Colorist Landscape Composer Three Selected Works for Oboe
No full textThis project examines three contrasting works, featuring the oboe by award-
winning American composer, Jenni Brandon. A champion of writing new music for the
oboe, Brandon has composed thirteen works from 2003 to 2016, ranging from
unaccompanied solo works to woodwind chamber ensemble.
Brandon is highly influenced by her surrounding landscapes and paints vivid
musical pictures. Apart from being emotionally impactful, Brandon’s works are enriched
with color, lyricism, and technical demand. This document focuses on three selected oboe
works by Brandon: On Holt Avenue (2006), In the City at Night (2008), and The Sequoia
Trio (2008). It presents a biographical sketch of the composer, surveys of the works,
compositional process and provide performance practice considerations. It is hoped that
this research will continue to increase awareness of the composer, provide performers a
better understanding on approaching the selected work, while promoting individual
expression.Embargo status: Restricted to TTU community only. To view, login with your eRaider (top right). Others may request the author grant access exception by clicking on the PDF link to the left
Improving community wellness through the inclusion of traditional knowledge: a participatory action research study in Nunavut
No full textNunavut communities struggle with a variety of social and emotional challenges, which are evidenced by elevated rates of: completed suicides among youth, childhood sexual assault, domestic violence and addiction. However, this struggle is not congruent with traditional Inuit culture, as many of these issues have only arisen since colonization occurred in the mid 1950’s. In an effort to account for this incongruence, this participatory action research project uses the methodology of descriptive phenomenology to interview ten elders from a Nunavut community.
These interviews enlighten the reader on the traditional knowledge and lived experience of elders (including historical trauma), and put modern Inuit culture into perspective. They also identify values and beliefs that have the potential to improve community wellness. The themes that emerge include: respect, leadership, family connection, inclusion of traditional knowledge, working together, and resiliency. Unsurprisingly, the project’s results are consistent with other community wellness research projects in Nunavut that recommend community-based solutions focused on resilience and strength. This project expands on this generalization to provide concrete solutions that communities can utilize to improve community wellness.Includes bibliographical references (pages 100-109).
"Thesis submitted in partial fulfillment of the requirements for the degree Master of Psychiatric Nursing in the Graduate Studies Program at Brandon University, School of Health Studies.
AAC Brandon Hard Red Spring Wheat
No full textAbstract:
AAC Brandon Hard Red Spring Wheat. Can. J. Plant Sci. ***** 2016.
AAC Brandon hard red spring wheat (Triticum aestivum L.) has grain yield and time to maturity within the range of the check cultivars: Katepwa, Laura, Lillian, Carberry, CDC Kernen. AAC Brandon has an awned spike, a low lodging score indicative of strong straw, and a short plant stature typical of a semidwarf. AAC Brandon expressed resistance to prevalent races of leaf rust, stem rust, and stripe rust, and moderate resistance to fusarium head blight, and loose smut. Compared to the five Canada Western Red Spring check cultivars, AAC Brandon has improved flour yield, and lower flower ash. AAC Brandon is eligible for grades of Canada Western Red Spring.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author
Patterns of Transcript Abundance of Eukaryotic Biogeochemically-Relevant Genes in the Amazon River Plume
No full textThe Amazon River has the largest discharge of all rivers on Earth, and its complex plume system fuels a wide array of biogeochemical processes, across a large area of the western tropical North Atlantic. The plume thus stimulates microbial processes affecting carbon sequestration and nutrient cycles at a global scale. Chromosomal gene expression patterns of the 2.0 to 156 μm size-fraction eukaryotic microbial community were investigated in the Amazon River Plume, generating a robust dataset (more than 100 million mRNA sequences) that depicts the metabolic capabilities and interactions among the eukaryotic microbes. Combining classical oceanographic field measurements with metatranscriptomics yielded characterization of the hydrographic conditions simultaneous with a quantification of transcriptional activity and identity of the community. We highlight the patterns of eukaryotic gene expression for 31 biogeochemically significant gene targets hypothesized to be valuable within forecasting models. An advantage to this targeted approach is that the database of reference sequences used to identify the target genes was selectively constructed and highly curated optimizing taxonomic coverage, throughput, and the accuracy of annotations. A coastal diatom bloom highly expressed nitrate transporters and carbonic anhydrase presumably to support high growth rates and enhance uptake of low levels of dissolved nitrate and CO2. Diatom-diazotroph association (DDA: diatoms with nitrogen fixing symbionts) blooms were common when surface salinity was mesohaline and dissolved nitrate concentrations were below detection, and hence did not show evidence of nitrate utilization, suggesting they relied on ammonium transporters to aquire recently fixed nitrogen. These DDA blooms in the outer plume had rapid turnover of the photosystem D1 protein presumably caused by photodegradation under increased light penetration in clearer waters, and increased expression of silicon transporters as silicon became limiting. Expression of these genes, including carbonic anhydrase and transporters for nitrate and phosphate, were found to reflect the physiological status and biogeochemistry of river plume environments. These relatively stable patterns of eukaryotic transcript abundance occurred over modest spatiotemporal scales, with similarity observed in sample duplicates collected up to 2.45 km in space and 120 minutes in time. These results confirm the use of metatranscriptomics as a valuable tool to understand and predict microbial community function.Gordon and Betty Moore Foundation (River Ocean Continuum of the Amazon Project. Grants GBMF 2293 and 2928)National Science Foundation (U.S.) (Grant NSF-OCE 0934095
High school teachers' attitudes towards inclusion: a Canadian perspective
No full textThis study investigated Canadian high school teachers’ attitudes towards inclusion of students with special education needs into the general classroom environment. Teachers’ cognitive, affective and behavioral attitudes towards inclusion were examined. Data was collected using a 7-point Likert scale survey called the Inclusion Scale for High School Teachers created by Dr. Catherine Ernst (2006). The survey, which included a demographic questionnaire as well as cognitive, affective and behavioral attitude statements regarding inclusive practices, was conducted with a population of 150 high school teachers from a single urban school division in a large city in central Canada. Participants’ demographic information was analyzed using descriptive statistics. The Somers' Delta (Somers’ D) statistic was used to determine the strength and relatedness of independent variables of teacher demographics and school environmental variables with the dependent variable of teacher attitude. Findings showed that high school teachers’ attitudes towards inclusion were more positive than negative. It was found that teachers’ behavioral attitudes towards inclusion were most positive while their affective attitudes towards inclusion were least positive. The demographic variables with the greatest influence on teacher attitudes towards inclusion were: (a) experience as lead teacher in an inclusive setting, (b) access to human resources and supports, and (c) professional development and training related to inclusion. This study is of particular importance as it is the first study to focus specifically on Canadian high school teachers’ attitudes towards inclusion.
Keywords: high school, teachers, inclusion, attitudeIncludes bibliographical references (pages 88-94)."In partial fulfillment of the requirements for the degree of Master of Education.
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