153 research outputs found
"Viva" (2014): a geographically Novel by Patrick Deville
No full textThis paper presents a Novel published in 2014 by the French author Patrick Deville entitled Viva. It studies the contemporary work written new and scholar, both geographical and historical, in three stages: the first shows the singularities of this travel story; these condexamines the singular vision (both intertextual and interdisciplinary) of Mexican's landscapes; the third explains with help of geocriticism theorythis new art of fictionalize space.Cet article présente le dernier roman en date de l'écrivain français Patrick Deville, Viva, publié en 2014. Il se propose d'analyser cette écriture romanesque contemporaine inédite et érudite, á la fois géographique et historique, selon un plan tripartite, en soulignant d'abord les singularités de ce récit de voyages, en donnant ensuite á lire la vision singuliére, intertextuelle et transartistique, des territoires mexicains et enfin en scrutant á l'aide des théories géocritiques ce nouvel art de fictionnaliser l'espace
"Viva" (2014): un roman géographique de Patrick Deville
No full textThis paper presents a Novel published in 2014 by the French author Patrick Deville entitled Viva. It studies the contemporary work written new and scholar, both geographical and historical, in three stages: the first shows the singularities of this travel story; these condexamines the singular vision (both intertextual and interdisciplinary) of Mexican’s landscapes; the third explains with help of geocriticism theorythis new art of fictionalize space.Cet article présente le dernier roman en date de l’écrivain français Patrick Deville, Viva, publié en 2014. Il se propose d’analyser cette écriture romanesque contemporaine inédite et érudite, à la fois géographique et historique, selon un plan tripartite, en soulignant d’abord les singularités de ce récit de voyages, en donnant ensuite à lire la vision singulière, intertextuelle et transartistique, des territoires mexicains et enfin en scrutant à l’aide des théories géocritiques ce nouvel art de fictionnaliser l’espace
KC 05: on destruction and preservation in creative process
No full textParticipating panellist at Kitchen Conversations London: Creative Destruction Series; no. 05: On Destruction and Preservation in Creative Process. Panellists include Alia Syed, Noski Deville, Architects Smout Allen and poet Agnieszka Studzinska.
Cinematographer Noski Deville and artist filmmaker Alia Syed discuss creation and destruction in their film collaboration 'Priya'(2011).
Architects Smout Allen, whose work Infractus: the taking of Robin Hood Gardens, deals with the controversial demolition of Alison and Peter Smithson’s 1972 housing estate in east London, join the conversation on destruction and preservation.
A rare opportunity to see Alia Syed’s film Priya (2011) projected from 16mm print.
Plus Poet Agnieszka Studzinska, author of Passage reading her work, launching Wapping Project's next publication of commissioned writing.
Kitchen Conversations London are presented in partnership and with generous support of The Future Laboratory
Dynamics of a fully stochastic discretized neuronal model with excitatory and inhibitory neurons
No full textWe consider here an extension and generalization of the stochastic
neuronal network model developed by DeVille et al.; their model
corresponded to an all-to-all network of discretized
integrate-and-fire excitatory neurons where synapses are
failure-prone. It was shown that this model exhibits different
metastable phases of asynchronous and synchronous behavior, since the
model limits on a mean-field deterministic system with multiple
attractors. Our work investigates adding inhibition into the
model. The new model exhibits the same metastable phases, but also
exhibits new non-monotonic behavior that was not seen in the DeVille
et al. model. The techniques used by DeVille et al. for finding the
mean-field limit are not suitable for this new model. We explore
early attempts at obtaining a new mean-field deterministic system that
would give us an understanding of the behavior seen in the new
model. After redefining the process we do find a mean-field
deterministic system that the model limits on, and we investigate the
behavior of the new model studying the mean-field system.Submission published under a 24 month embargo labeled 'U of I only', the embargo will last until 2017-08-01The student, Stephen Berning, accepted the attached license on 2015-07-15 at 01:11.The student, Stephen Berning, submitted this Dissertation for approval on 2015-07-15 at 01:27.This Dissertation was approved for publication on 2015-07-16 at 13:11.DSpace SAF Submission Ingestion Package generated from Vireo submission #8443 on 2015-09-29 at 14:59:30Made available in DSpace on 2015-09-29T20:49:56Z (GMT). No. of bitstreams: 2
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Previous issue date: 2015-07-16Embargo set by: Seth Robbins for item 89469
Lift date: 2017-09-29T20:50:34Z
Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD systemU of I Only Restriction Lifted for Item 89469 on 2017-09-30T09:15:18Z
Investigating the effect of poly-l-lactic acid nanoparticles carrying hypericin on the flow-biased diffusive motion of HeLa cell organelles
No full textObjectives In this study, we investigate in human cervical epithelial HeLa cells the intracellular dynamics and the mutual interaction with the organelles of the poly-l-lactic acid nanoparticles (PLLA NPs) carrying the naturally occurring hydrophobic photosensitizer hypericin. Methods Temporal and spatiotemporal image correlation spectroscopy was used for the assessment of the intracellular diffusion and directed motion of the nanocarriers by tracking the hypericin fluorescence. Using image cross-correlation spectroscopy and specific fluorescent labelling of endosomes, lysosomes and mitochondria, the NPs dynamics in association with the cell organelles was studied. Static colocalization experiments were interpreted according to the Manders' overlap coefficient. Key findings Nanoparticles associate with a small fraction of the whole-organelle population. The organelles moving with NPs exhibit higher directed motion compared to those moving without them. The rate of the directed motion drops substantially after the application of nocodazole. The random component of the organelle motions is not influenced by the NPs. Conclusions Image correlation and cross-correlation spectroscopy are most appropriate to unravel the motion of the PLLA nanocarrier and to demonstrate that the rate of the directed motion of organelles is influenced by their interaction with the nanocarriers. Not all PLLA-hypericin NPs are associated with organelles.</p
Stability bounds for nonlinear dispersive Hamiltonian partial differential equations
No full textSubmission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2024-03-01 without embargo termsThe student, Sarah Simpson, accepted the attached license on 2023-11-26 at 19:56.The student, Sarah Simpson, submitted this Dissertation for approval on 2023-11-26 at 20:18.This Dissertation was approved for publication on 2023-11-27 at 11:46.DSpace SAF Submission Ingestion Package generated from Vireo submission #20006 on 2024-03-01 at 13:14:59Existing approaches for analyzing the stability of periodic traveling wave solutions to dispersive partial differential equations (PDEs) often rely on numerical methods. However, due to computational limitations, these methods require an implicit assumption that all instabilities lie within a bounded region containing the origin. Recent research has revealed that this assumption does not always hold true, as in certain cases, the spectrum extends to infinity along curves having non-zero real part. Numerical methods are also susceptible to missing spectrum with small real part located further up the imaginary axis. To address these concerns, explicit bounds indicating the region within which all instabilities must be contained would be valuable. This dissertation focuses on providing such bounds for a specific subset of these problems, namely, dispersive, Hamiltonian PDEs. We provide explicit bounds for the region within which all instabilities must lie, along with giving an upper bound on the number of instabilities. For cases where the dispersion relation exhibits at least cubic growth rate these bounds are obtained via a Gershgorin disc theorem type argument coupled with application of the fact that the spectrum of Hamiltonian operators is symmetric with respect to reflection across both the real and imaginary axes. In instances where the growth rate of the dispersion relation is only quadratic we provide an alternative argument following the form of a second-order perturbation calculation to bound the desired region. We provide these results in a general form readily available for applications along with discussing several specific examples in detail including generalized Korteweg-de Vries, Benjamin-Bona-Mahony, Kawahara, and generalized Benjamin-Ono
Comparison of extracellular vesicle isolation and storage methods using high-sensitivity flow cytometry
No full textExtracellular vesicles (EVs) are of interest for a wide variety of biomedical applications. A major limitation for the clinical use of EVs is the lack of standardized methods for the fast and reproducible separation and subsequent detection of EV subpopulations from biofluids, as well as their storage. To advance this application area, fluorescence-based characterization technologies with single-EV resolution, such as high-sensitivity flow cytometry (HS-FCM), are powerful to allow assessment of EV fractionation methods and storage conditions. Furthermore, the use of HS-FCM and fluorescent labeling of EV subsets is expanding due to the potential of high-throughput, multiplex analysis, but requires further method development to enhance the reproducibility of measurements. In this study, we have applied HS-FCM measurements next to standard EV characterization techniques, including nanoparticle tracking analysis, to compare the yield and purity of EV fractions obtained from lipopolysaccharide-stimulated monocytic THP-1 cells by two EV isolation methods, differential centrifugation followed by ultracentrifugation and the exoEasy membrane affinity spin column purification. We observed differences in EV yield and purity. In addition, we have investigated the influence of EV storage at 4 degrees C or -80 degrees C for up to one month on the EV concentration and the stability of EV-associated fluorescent labels. The concentration of the in vitro cell derived EV fractions was shown to remain stable under the tested storage conditions, however, the fluorescence intensity of labeled EV stored at 4 degrees C started to decline within one day.</p
Intravesicular Genomic DNA Enriched by Size Exclusion Chromatography Can Enhance Lung Cancer Oncogene Mutation Detection Sensitivity
No full textExtracellular vesicles (EVs) are cell-derived structures surrounded by a lipid bilayer that carry RNA and DNA as potential templates for molecular diagnostics, e.g., in cancer genotyping. While it has been established that DNA templates appear on the outside of EVs, no consensus exists on which nucleic acid species inside small EVs (<200 nm, sEVs) are sufficiently abundant and accessible for developing genotyping protocols. We investigated this by extracting total intravesicular nucleic acid content from sEVs isolated from the conditioned cell medium of the human NCI-H1975 cell line containing the epidermal growth factor (EGFR) gene mutation T790M as a model system for non-small cell lung cancer. We observed that mainly short genomic DNA (<35–100 bp) present in the sEVs served as a template. Using qEV size exclusion chromatography (SEC), significantly lower yield and higher purity of isolated sEV fractions were obtained as compared to exoEasy membrane affinity purification and ultracentrifugation. Nevertheless, we detected the EGFR T790M mutation in the sEVs’ lumen with similar sensitivity using digital PCR. When applying SEC-based sEV separation prior to cell-free DNA extraction on spiked human plasma samples, we found significantly higher mutant allele frequencies as compared to standard cell-free DNA extraction, which in part was due to co-purification of circulating tumor DNA. We conclude that intravesicular genomic DNA can be exploited next to ctDNA to enhance EGFR T790M mutation detection sensitivity by adding a fast and easy-to-use sEV separation method, such as SEC, upstream of standard clinical cell-free DNA workflows
Macrophages Release Extracellular Vesicles of Different Properties and Composition Following Exposure to Nanoparticles
No full textExtracellular vesicles are membrane-bound carriers with complex cargoes, which play a major role in intercellular communication, for instance, in the context of the immune response. Macrophages are known to release extracellular vesicles in response to different stimuli, and changes in their size, number, and composition may provide important insights into the responses induced. Macrophages are also known to be highly efficient in clearing nanoparticles, when in contact with them, and in triggering the immune system. However, little is known about how the nature and composition of the vesicles released by these cells may vary upon nanoparticle exposure. In order to study this, in this work, alveolar-like macrophages were exposed to a panel of nanoparticles with varying surface and composition, including amino-modified and carboxylated polystyrene and plain silica. We previously showed that these nanoparticles induced very different responses in these cells. Here, experimental conditions were carefully tuned in order to separate the extracellular vesicles released by the macrophages several hours after exposure to sub-toxic concentrations of the same nanoparticles. After separation, different methods, including high-sensitivity flow cytometry, TEM imaging, Western blotting, and nanoparticle tracking analysis, were combined in order to characterize the extracellular vesicles. Finally, proteomics was used to determine their composition and how it varied upon exposure to the different nanoparticles. Our results show that depending on the nanoparticles’ properties. The macrophages produced extracellular vesicles of varying number, size, and protein composition. This indicates that macrophages release specific signals in response to nanoparticles and overall suggests that extracellular vesicles can reflect subtle responses to nanoparticles and nanoparticle impact on intercellular communication
Transient loading of CD34+ hematopoietic progenitor cells with polystyrene nanoparticles
No full textSarah Deville,1,2 Wahyu Wijaya Hadiwikarta,1 Nick Smisdom,1,2 Bart Wathiong,1,3 Marcel Ameloot,2 Inge Nelissen,1 Jef Hooyberghs1,3 1VITO, Flemish Institute for Technological Research, Mol, Belgium; 2Biomedical Research Institute, Hasselt University, Diepenbeek, Belgium; 3Theoretical Physics, Hasselt University, Diepenbeek, Belgium Abstract: CD34+ hematopoietic progenitor cells (HPCs) offer great opportunities to develop new treatments for numerous malignant and non-malignant diseases. Nanoparticle (NP)-based strategies can further enhance this potential, and therefore a thorough understanding of the loading behavior of HPCs towards NPs is essential for a successful application. The present study focusses on the interaction kinetics of 40 nm sized carboxylated polystyrene (PS) NPs with HPCs. Interestingly, a transient association of the NPs with HPCs is observed, reaching a maximum within 1 hour and declining afterwards. This behavior is not seen in dendritic cells (CD34-DCs) differentiated from HPCs, which display a monotonic increase in NP load. We demonstrate that this transient interaction requires an energy-dependent cellular process, suggesting active loading and release of NPs by HPCs. This novel observation offers a unique approach to transiently equip HPCs. A simple theoretical approach modeling the kinetics of NP loading and release is presented, contributing to a framework of describing this phenomenon. Keywords: nanoparticles, hematopoietic progenitor cells, dendritic cells, uptake, releas
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