132 research outputs found
Selection of sAPRIL-BP.
No full text<p>(A) The binding affinity of phage clones No.1–20 for sAPRIL were determined by ELISA. Clone 21 was used as a positive control. The fold change of the optical density was normalized to the positive control. Clones that had at least a 6-fold greater affinity than the positive control were considered ‘positive’ for sAPRIL binding. (B) Three binding peptides were synthesized and their binding affinity with sAPRIL (black bars) was determined and compared with the negative control (NC) using ELISA. Cross-reactivity was assessed by measuring the binding affinity to BAFF (grey bars). (C) Clone BP1 (sAPRIL-BP) was mixed with sAPRIL at different doses to compete for binding with fixed LOVO cells.</p
Identification of the sAPRIL binding peptide and its growth inhibition effects in the colorectal cancer cells.
No full textA proliferation-inducing ligand (APRIL) is a member of the tumor necrosis factor (TNF) super family. It binds to its specific receptors and is involved in multiple processes during tumorigenesis and tumor cells proliferation. High levels of APRIL expression are closely correlated to the growth, metastasis, and 5-FU drug resistance of colorectal cancer. The aim of this study was to identify a specific APRIL binding peptide (BP) able to block APRIL activity that could be used as a potential treatment for colorectal cancer.A phage display library was used to identify peptides that bound selectively to soluble recombinant human APRIL (sAPRIL). The peptides with the highest binding affinity for sAPRIL were identified using ELISA. The effects of sAPRIL-BP on cell proliferation and cell cycle/apoptosis in vitro were evaluated using the CCK-8 assay and flow cytometry, respectively. An in vivo mouse model of colorectal cancer was used to determine the anti-tumor efficacy of the sAPRIL-BP.Three candidate peptides were characterized from eight phage clones with high binding affinity for sAPRIL. The peptide with the highest affinity was selected for further characterization. The identified sAPRIL-BP suppressed tumor cell proliferation and cell cycle progression in LOVO cells in a dose-dependent manner. In vivo in a mouse colorectal challenge model, the sAPRIL-BP reduced the growth of tumor xenografts in nude mice by inhibiting proliferation and inducing apoptosis intratumorally. Moreover, in an in vivo metastasis model, sAPRIL-BP reduced liver metastasis of colorectal cancer cells.sAPRIL-BP significantly suppressed tumor growth in vitro and in vivo and might be a candidate for treating colorectal cancers that express high levels of APRIL
Serological markers “CEA test & sAPRIL test” in Iraqi patients with colon cancer
Get PDFBackground: Colonic cancer is a very common disease world-wide being fourth most common cancer characterized by abnormal proliferation of the inner wall of colon then taking full colon wall thickness then spreading to surrounding lymph nodes and tissues and finally distant metastasis. It is one of most complicated diseases with debilitating symptoms which becomes more sever , prominent and specific with advancing stage with high percent of fatality and relatively short survival if diagnosed late or if left untreated.
Objective: To evaluate the efficacy of serum CEA & sAPRIL levels in the diagnosis and screening of colon cancer and their validity for this.
Patients and methods: This study was applied on 35 patients with colonic cancer, 35 patients with benign polyps and 15 negative controls. All individuals were subjected to blood sampling for measuring their serum CEA & sAPRIL using ELISA technique.
Results: In this study, majority of patients with colon cancer were presented at ages between 53-82 years of age (mean 68.5±6.4 years). Serum levels of sAPRIL & CEA were significantly elevated in those patients with advancing stages (C & D) compared with stages (A & B) and lower levels were found in patients who had surgical removal of tumor or received chemotherapy. Also a positive relation was found between sAPRIL & CEA with alcohol intake and smoking
Conclusion: according to this study sample it was found that sAPRIL and CEA together are strong indicators for colon cancer screening & diagnosis, and by this will reduce the need for more invasive screening & diagnostic tools
Effect of sAPRIL-BP on cell cycle and apoptosis of LOVO cells.
No full text<p>LOVO cells were treated with the indicated doses of sAPRIL-BP for 48 h. Cells were stained with PI for cell cycle analysis (A and B) and PI + Annexin V for apoptosis analysis (C and D) by flow cytometry. *<i>p</i> <0.05 compared to Vehicle control; #<i>p</i> <0.05 compared to the low dose group.</p
<i>In vivo</i> effect of sAPRIL-BP on liver metastasis.
No full text<p>LOVO cells were injected into the spleens of nude mice to observe experimental liver metastasis. Three weeks after injection, the mice were divided into 3 groups (N = 5) and treated with PBS (control), low (20 mg/kg), or high (40 mg/kg) doses of sAPRIL-BP every other day. Mice were sacrificed after two weeks of treatment with sAPRIL-BP. (A) Representative pictures of the metastatic liver tumors from each group are shown. (B) Numbers of metastatic nodules per mouse were recorded. *<i>P</i> <0.05 compared to control. #<i>P</i> <0.05 compared to the low dose group. (C) Numbers of metastatic nodules with the indicated size were recorded. Total numbers of metastatic nodules: n = 197 (Con), n = 130 (20 mg/kg), and n = 84 (40 mg/kg).</p
<i>In vivo</i> effects of sAPRIL-BP on tumor development.
No full text<p>LOVO cells were injected subcutaneously into nude mice and allowed to grow for 3 weeks. Once the tumor was establishes, the mice were divided into 3 groups (N = 5) and treated with PBS (control), low (20 mg/kg), or high (40 mg/kg) dose of sAPRIL-BP every other day. (A) Representative examples of the tumors from each group are shown. Top panel: Scale bar, 8 mm. Bottom panel: Scale bar, 7 mm. (B) Mice were sacrificed after two weeks of treatment with sAPRIL-BP and the tumor weights were recorded. *<i>p</i> <0.05 compared to control. #<i>p</i> <0.05 compared to the low dose group. (C) The tumor volume was recorded every two days during treatment. *<i>p</i> <0.05 compared to control.</p
Analysis of the correlation between Th1 cytokines and sBAFF/sAPRIL. A
No full text<p>. The levels of IFN-γ, IL-12p70 and IL-2 were detected in plasma (HD, n = 15; LTB<sub>L</sub>, n = 11; LTB<sub>H</sub>, n = 14; TB, n = 11) and pleural effusion (TP, n = 10) using Luminex technology. <b>B</b>. Significant positive correlations were identified between sAPRIL, and IFN-γ (Pearson’s correlation coefficient, <i>r</i> = 0.8412, <i>p</i> = 0.0023), IL-12p70 (Pearson’s correlation coefficient <i>r</i> = 0.8211, <i>p</i> = 0.0036) and IL-2 (Pearson’s correlation coefficient <i>r</i> = 0.7424, <i>p</i> = 0.0139) in the TP pleural effusion.</p
Effect of sAPRIL-BP on the proliferation of LOVO and SW620 cells.
No full text<p>(A) APRIL<sup>high</sup> LOVO and HCT116 cells and (B) APRIL<sup>low</sup> SW620 and HT-29 cells were treated with the indicated doses of sAPRIL binding peptides for 24, 48, and 72 h, and proliferation was determined using the CCK-8 kit. The rate of proliferation inhibition was calculated as: (%) = [(mean of OD<sub>control</sub>—mean of OD<sub>experimental</sub>) / mean of OD<sub>control</sub>]×100%.</p
KOMPETENSI MANAJERIAL KEPALA SEKOLAH DALAM PENINGKATAN MUTU PEMBELAJARAN DI BALAI PENDIDIKAN DAN PELATIHAN ILMU PELAYARAN MALAHAYATI ACEH DAN SEKOLAH USAHA PERIKANAN MENENGAH NEGERI LADONG ACEH
Get PDFKOMPETENSI MANAJERIAL KEPALA SEKOLAHDALAM PENINGKATAN MUTU PEMBELAJARANDI BALAI PENDIDIKAN DAN PELATIHAN ILMUPELAYARAN MALAHAYATI ACEH DANSEKOLAH USAHA PERIKANANMENENGAH NEGERILADONG ACEHOleh: Muhammad Sapril SiregarNPM: 1609200050050Komisi Pembimbing:1.Prof. Dr. Yusrizal, M. Pd.2.Dr. Bahrun, M. Pd.ABSTRAKSeorang kepala sekolah harus memiliki kompetensi dalam memimpin organisasi pendidikan di sekolah, salah satu kompetensi yang sangat mendukung dalam kepemimpinannya yaitu kompetensi manajerial. Penelitian ini bertujuan untukmengetahui kompetensi manajerial kepala sekolah dalam peningkatan mutu pembelajaran di BP2IP Malahayati Aceh dan SUPM Negeri Ladong Aceh melalui program, strategi dan hambatan yang dihadapi kepala sekolah dalam meningkatkan mutu pembelajarandi BP2IP Malahayati Aceh dan SUPM Negeri Ladong. Penelitian yang digunakan yaitu pendekatan kualitatif dengan metode deskriptif. Subjek dalam penelitian ini meliputi kepala sekolah, guru dan siswa sebagai peserta didik. Pengumpulan data dilakukan dengan cara observasi langsung, wawancara mendalam dan studi dokumentasi. Teknik analisis data melalui tiga tahap yaitu reduksi data, penyajian data dan verifikasi. Hasil penelitian menunjukkan bahwa:(1) Kepala BP2IP Malahayati Aceh dan SUPM Negeri Ladong Aceh telah membuat program dalam meningkatkan mutu pembelajaran; (2) kepala BP2IP Malahayati Aceh dan SUPM Negeri Ladong Aceh telah memiliki strategi untuk mengsukseskan program yang telah dibuat dalam meningkatkan mutu pembelajaran; (3) kepala BP2IP Malahayati Aceh dan SUPM Negeri Ladong Aceh memiliki beberapa hambatan yang harus segera dievaluasi untuk perbaikan kedepannya dalam meningkatkan mutu pembelajaran.Kata Kunci: Program, Strategi dan Hambata
Two Gln187 mutants of human soluble APRIL inhibit proliferation of lung carcinoma A549 cells
No full textSoluble APRIL (sAPRIL), the active form of a proliferation-inducing ligand (APRIL), is implicated in the proliferation of tumor cells. Suppressing APRIL function has been considered as a potential strategy for the therapy of APRIL-associated tumors. In the present study, we generated human sAPRIL and its two mutants, Gln187-D-sAPRIL (Gln187 deleted) and Gly187-sAPRIL (Gln187 replaced by Gly). In vitro experiments showed that the two mutants had similar specific binding capacity to lung carcinoma A549 cells compared to the wild-type sAPRIL, and both, especially Gly187-sAPRIL, exhibited significant antagonistic effect on sAPRIL-induced tumor cell proliferation in a dose-dependent manner, which might be predominantly mediated by blocking sAPRIL-induced MEK and ERK phosphorylation but not p38MAPK or JNK signaling. In vivo experiments with nude mice bearing A549 cell-derived xenograft tumor showed that only the Gly187-sAPRIL mutant could significantly suppress the tumor growth. These results suggest that Gln187 may be a crucial amino acid in APRIL-mediated tumor cell proliferation via the MEK-ERK signaling pathway and that the sAPRIL mutants may serve as novel potential antagonists of APRIL for the therapy of APRIL-associated cancers
- …
