1,720,958 research outputs found
Proteomika jako nástroj pro zkoumání adaptivních strategií u protistů
No full textProteomics provides a unique and powerful framework to investigate the molecular basis of adaptive strategies in diverse protists, ranging from marine phytoplankton to parasitic protozoa. This thesis focuses on advanced proteomic methods to explore how these organisms respond to environmental and biological pressures at the molecular level. In marine eukaryotic phytoplankton, proteomic analyses uncovered the dynamic regulation of iron acquisition mechanisms, including phytotransferrins, siderophore uptake systems, and iron-responsive metabolic adaptations. Species-specific studies demonstrated rapid modulation of these proteins following changes in iron availability, while multispecies coculture experiments showed how iron scarcity restructures algal consortia composition by favoring species adapted to low-nutrient environments. In parasitic protists, including apicomplexans, trypanosomes, and free-living pathogenic amoebae, proteomics illuminated how parasites dynamically remodel their protein expression during life cycle transitions and in response to interaction with the host. Techniques such as proximity labeling and hyperLOPIT revealed novel proteins involved in host cell invasion, intracellular survival, and immune evasion. In Naegleria fowleri, comparative proteomics identified...Proteomika poskytuje jedinečný a silný nástroj pro zkoumání adaptačních strategií u různých protistů, od mořského fytoplanktonu až po parazitické prvoky. Tato práce se zaměřuje na pokročilé proteomické metody a jejich využití pro zkoumání, jak tyto organismy reagují na environmentální a biologické tlaky na molekulární úrovni. U mořského eukaryotického fytoplanktonu proteomická analýza odhalila dynamickou regulaci mechanismů příjmu železa, včetně proteinů jako fytotransferrin, regulaci systémů pro příjem sideroforů a metabolických adaptací reagujících na dostupnost železa. Studie zaměřené na jednotlivé druhy ukázaly rychlou modulaci adaptačních mechanismů při změně v dostupnosti železa, zatímco experimenty s kokulturami více druhů prokázaly, jak nedostatek železa přetváří složení těchto řasových konsorcií ve prospěch druhů přizpůsobených prostředí s nízkým obsahem živin. U parazitických protistů, včetně apikomplex, trypanosom a volně žijících patogenních améb, proteomika ozřejmila, jak parazité dynamicky přetvářejí expresi proteinů během životního cyklu a v reakci na interakce s hostitelem. Proteomické techniky jako proximity labeling a hyperLOPIT odhalily nové proteiny zapojené do invaze hostitelských buněk, intracelulárního přežívání a úniku před imunitní odpovědí. U Naegleria fowleri srovnávací...Katedra parazitologieDepartment of ParasitologyFaculty of SciencePřírodovědecká fakult
Proteomics as a tool for exploring adaptive strategies in protists
No full textProteomics provides a unique and powerful framework to investigate the molecular basis of adaptive strategies in diverse protists, ranging from marine phytoplankton to parasitic protozoa. This thesis focuses on advanced proteomic methods to explore how these organisms respond to environmental and biological pressures at the molecular level. In marine eukaryotic phytoplankton, proteomic analyses uncovered the dynamic regulation of iron acquisition mechanisms, including phytotransferrins, siderophore uptake systems, and iron-responsive metabolic adaptations. Species-specific studies demonstrated rapid modulation of these proteins following changes in iron availability, while multispecies coculture experiments showed how iron scarcity restructures algal consortia composition by favoring species adapted to low-nutrient environments. In parasitic protists, including apicomplexans, trypanosomes, and free-living pathogenic amoebae, proteomics illuminated how parasites dynamically remodel their protein expression during life cycle transitions and in response to interaction with the host. Techniques such as proximity labeling and hyperLOPIT revealed novel proteins involved in host cell invasion, intracellular survival, and immune evasion. In Naegleria fowleri, comparative proteomics identified..
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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