42,022 research outputs found
Erratum to: Effect of moderate red wine intake on cardiac prognosis after recent acute myocardial infarction of subjects with Type 2 diabetes mellitus (Diabetic Medicine, (2006), 23, 9, (974-981), 10.1111/j.1464-5491.2006.01886.x)
No full textIn an article by Marfella et al, the author name C. Saron is incorrect and should be listed as C. Sardu. Therefore the correct author list is: R. Marfella, F. Cacciapuoti, M. Siniscalchi, F. C. Sasso, F. Marchese, F. Cinone, E. Musacchio, M. A. Marfella, L. Ruggiero, G. Chiorazzo, D. Liberti, G. Chiorazzo, G. F. Nicoletti, C. Sardu, F. D'Andrea, C. Ammendola, M. Verza and L. Coppola.In an article by Marfella et al, the author name C. Saron is incorrect and should be listed as C. Sardu. Therefore the correct author list is: R. Marfella, F. Cacciapuoti, M. Siniscalchi, F. C. Sasso, F. Marchese, F. Cinone, E. Musacchio, M. A. Marfella, L. Ruggiero, G. Chiorazzo, D. Liberti, G. Chiorazzo, G. F. Nicoletti, C. Sardu, F. D'Andrea, C. Ammendola, M. Verza and L. Coppola
The nickel site of Bacillus pasteurii UreE, a urease metallo-chaperone, as revealed by metal-binding studies and X-ray absorption spectroscopy
No full textUreE is a homodimeric metallo-chaperone that assists the insertion of Ni2+ ions in the active site of urease. The crystal structures of UreE from Bacillus pasteurii and Klebsiella aerogenes have been determined, but the details of the nickel-binding site were not elucidated due to solid-state effects that caused disorder in a key portion of the protein. A complementary approach to this problem is described here. Titrations of wild-type Bacillus pasteurii UreE (BpUreE) with Ni2+, followed by metal ion quantitative analysis using inductively coupled plasma optical emission spectrometry (ICP-OES), established the binding of 2 Ni2+ ions to the functional dimer, with an overall dissociation constant K-D = 35 mu M. To establish the nature, the number, and the geometry of the ligands around the Ni2+ ions in BpUreE-Ni-2, X-ray absorption spectroscopy data were collected and analyzed using an approach that combines ab initio extended X-ray absorption fine structure (EXAFS) calculations with a systematic search of several possible coordination geometries, using the Simplex algorithm. This analysis indicated the presence of Ni2+ ions in octahedral coordination geometry and an average of two histidine residues and four O/N ligands bound to each metal ion. The fit improved significantly with the incorporation, in the model, of a Ni-O-Ni moiety, suggesting the presence of a hydroxide-bridged dinuclear cluster in the Ni-loaded BpUreE. These results were interpreted using two possible models. One model involves the presence of two identical metal sites binding Ni2+ with negative cooperativity, with each metal ion bound to the conserved His(100) as well as to either His(145) or His(147) from each monomer, residues found largely conserved at the C-terminal. The alternative model comprises the presence of two different binding sites featuring different affinity for Ni2+. This latter model would involve the presence of a dinuclear metallic core, with one Ni2+ ion bound to one His(100) from each monomer, and the second Ni2+ ion bound to a pair of either His(145) or His(147). The arguments in favor of one model as compared to the other are discussed on the basis of the available biochemical data
[Memo from Lieutenant Colonel M. F. Hass, Civil Affairs Division, with amendments to an evacuation proposal]
No full textA memorandum sent form Lieutenant General M. F. Hass, Civil Affairs Division, which has two corrections from a an evacuation proposal originally sent on May 13, 1942. The correction changes the destination to the Merced Assembly Center.The War Relocation Authority (WRA), together with the Wartime Civil Control Administration (WCCA), the Civil Affairs Division (CAD) and the Office of the Commanding General (OFG) of the Western Defense Command (WDC) operated together to segregate and house some 110,000 men women and children from 1942 to 1945. The collection contains documents and photographs relating to the establishment and administrative workings of the (WDC), the (WRA) and the (WCCA) for the year 1942
Corrigendum to “Presence and function of kisspeptin/KISS1R system in swine ovarian follicles” (Theriogenology (2018) 115 (1–8), (S0093691X1830147X), (10.1016/j.theriogenology.2018.04.006))
No full textThe authors regret the following changes to the author group G. Basinia, F. Grassellia, S. Bussolatia, R. Ciccimarraa, M. Maranesib, A. Bufalarib, C. Dall'Agliob, F. Parilloc,#, M. Zeranib,c,*. a Dipartimento di Scienze Mediche Veterinarie, Università di Parma, 43126 Parma, Italy. b Dipartimento di Medicina Veterinaria, Università di Perugia, 06126 Perugia Italy. c Scuola di Bioscienze e Medicina Veterinaria, Università di Camerino, 62024 Matelica Italy. # Deceased. * Corresponding author: tel.: +39 0755857642; fax +39 0755857654. E-mail address: [email protected] (M. Zerani). And to the acknowledgements and figures
[Memo from Lieutenant Colonel M. F. Hass, Civil Affairs Division, with amendments to an evacuation proposal]
No full textA memorandum sent form Lieutenant General M. F. Hass, Civil Affairs Division, which has two corrections from a an evacuation proposal originally sent on May 11, 1942. The first correction changes the number of evacuees to 275 people, and the second correction changes the destination to Tanforan Assembly Center.The War Relocation Authority (WRA), together with the Wartime Civil Control Administration (WCCA), the Civil Affairs Division (CAD) and the Office of the Commanding General (OFG) of the Western Defense Command (WDC) operated together to segregate and house some 110,000 men women and children from 1942 to 1945. The collection contains documents and photographs relating to the establishment and administrative workings of the (WDC), the (WRA) and the (WCCA) for the year 1942
[Memo from Lietnenant Colonel M. F. Hass, Civil Affairs Division, regarding the destination for Exclusion Order No. 98]
No full textA one page memo from Lieutenant Colonel M. F. Hass, Civil Affairs Division, that declares the destination for evacuees affected by Exclusion Order No. 98. The evacuees from all three movements were sent to the Portaln Assembly Center in Oregon.The War Relocation Authority (WRA), together with the Wartime Civil Control Administration (WCCA), the Civil Affairs Division (CAD) and the Office of the Commanding General (OFG) of the Western Defense Command (WDC) operated together to segregate and house some 110,000 men women and children from 1942 to 1945. The collection contains documents and photographs relating to the establishment and administrative workings of the (WDC), the (WRA) and the (WCCA) for the year 1942
Nicotinic acetylcholine receptors in rat forebrain that bind ¹⁸F-nifene: relating PET imaging, autoradiography, and behavior
No full textNicotinic acetylcholine receptors (nAChRs) in the brain are important for cognitive function; however, their specific role in relevant brain regions remains unclear. In this study, we used the novel compound ¹⁸F-nifene to examine the distribution of nAChRs in the rat forebrain, and for individual animals related the results to behavioral performance on an auditory-cognitive task. We first show negligible binding of ¹⁸F-nifene in mice lacking the β2 nAChR subunit, consistent with previous findings that ¹⁸F-nifene binds to α4β2* nAChRs. We then examined the distribution of ¹⁸F-nifene in rat using three methods: in vivo PET, ex vivo PET and autoradiography. Generally, ¹⁸F-nifene labeled forebrain regions known to contain nAChRs, and the three methods produced similar relative binding among regions. Importantly, ¹⁸F-nifene also labeled some white matter (myelinated axon) tracts, most prominently in the temporal subcortical region that contains the auditory thalamocortical pathway. Finally, we related ¹⁸F-nifene binding in several forebrain regions to each animal's performance on an auditory-cued, active avoidance task. The strongest correlations with performance after 14 days training were found for ¹⁸F-nifene binding in the temporal subcortical white matter, subiculum, and medial frontal cortex (correlation coefficients, r > 0.8); there was no correlation with binding in the auditory thalamus or auditory cortex. These findings suggest that individual performance is linked to nicotinic functions in specific brain regions, and further support a role for nAChRs in sensory-cognitive function.Peer reviewedAuthor's Manuscript is also available open access in PubMed Central: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3292694.This is the peer reviewed version of the following article: Bieszczad, K. M., Kant, R., Constantinescu, C. C., Pandey, S. K., Kawai, H. D., Metherate, R., Weinberger, N. M. and Mukherjee, J. (2012), Nicotinic acetylcholine receptors in rat forebrain that bind 18F-nifene: Relating PET imaging, autoradiography, and behavior. Synapse, 66: 418–434. doi: 10.1002/syn.21530, which has been published in final form at http://dx.doi.org/10.1002/syn.21530. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving
[Memo from Lieutenant Colonel M. F. Hass, Civil Affairs Division, about the evacuation schedule from Sacramento County]
No full textA one page memo from Lieutenant Colonel M. F. Hass, Civil Affairs Division, with an adjusted evacuation schedule related to Exclusion Order No. 93. The memo states that the adjusted schedule was due to requests from Japanese families to be reunited in the Sacramento Assembly Center. The evacuation dates are from May 27 to May 29, 1942.The War Relocation Authority (WRA), together with the Wartime Civil Control Administration (WCCA), the Civil Affairs Division (CAD) and the Office of the Commanding General (OFG) of the Western Defense Command (WDC) operated together to segregate and house some 110,000 men women and children from 1942 to 1945. The collection contains documents and photographs relating to the establishment and administrative workings of the (WDC), the (WRA) and the (WCCA) for the year 1942
[Memo from Lieutenant Colonel M. F. Hass, Civil Affairs Division, regarding the evaucation schedule for Exclusion Order No. 92]
No full textA one page memo from Lieutenant Colonel M. F. Hass, Civil Affairs Division, with an adjusted evacuation schedule related to Exclusion Order No. 93. Due to over-registration in the Sacramento area, the evacuation schedule was revised. The memo includes the number of people to be evacuated between May 27 and May 29, 1942.The War Relocation Authority (WRA), together with the Wartime Civil Control Administration (WCCA), the Civil Affairs Division (CAD) and the Office of the Commanding General (OFG) of the Western Defense Command (WDC) operated together to segregate and house some 110,000 men women and children from 1942 to 1945. The collection contains documents and photographs relating to the establishment and administrative workings of the (WDC), the (WRA) and the (WCCA) for the year 1942
[Memo from M. F. Hass, Lieutenant Colonel, regarding over-registration under Executive Order No. 54]
No full textA memo sent from Lieutenant Colonel M. F. Hass regarding over-registration due to Exclusion Order No. 54. The memo details the number of evacuees per day between May 12 and May 14, 1942, including the assembly center destination.The War Relocation Authority (WRA), together with the Wartime Civil Control Administration (WCCA), the Civil Affairs Division (CAD) and the Office of the Commanding General (OFG) of the Western Defense Command (WDC) operated together to segregate and house some 110,000 men women and children from 1942 to 1945. The collection contains documents and photographs relating to the establishment and administrative workings of the (WDC), the (WRA) and the (WCCA) for the year 1942
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