266 research outputs found
Education Architecture: an analysis of municipal early childhood schools by Mayumi Watanabe de Souza Lima
No full textEsta dissertação de mestrado tem como objetivo a análise das transformações espaciais dos espaços educativos das Escolas Municipais de Ensino Infantil (EMEI) projetadas pela equipe do Centro de Desenvolvimento de Equipamentos Urbanos e Comunitários (CEDEC), sob direção da arquiteta Mayumi Watanabe de Souza Lima, entre os anos de 1990 a 1992, em São Paulo, durante o mandato de Luiza Erundina (1989 - 1992). Esta pesquisa situa-se primordialmente na grande área de Ciências Sociais Aplicadas (Arquitetura, Urbanismo e Design) e caracteriza-se pela abordagem qualitativa. Utiliza-se, para a análise, de dados obtidos por meio de entrevistas semiestruturadas, de projetos arquitetônicos, da análise gráfica e de documentação primária. Propõe-se, portanto, estudar as alterações projetuais que ocorreram ao longo dos anos nas escolas construídas com a tecnologia de argamassa pré- -moldada. Com ênfase nas áreas externas e de brincar, pretende-se investigar as diferenças projetuais que existem entre o que existe atualmente e os projetos originais pensados e realizados pela equipe do CEDEC. Esta análise procura refletir sobre as alterações espaciais e construtivas e como sua nova organização espacial reflete diretamente no edifício e no entorno. Também serão discutidas as alterações dos programas de necessidades e espaços dedicados ao brincar nos projetos selecionados, suas adaptações ou, em alguns casos, supressões. Em última instância, o presente trabalho busca contribuir com os estudos sobre a trajetória na docência e funcionalismo público e das produções de Watanabe, autora que ocupa hoje lugar ainda pouco destacado na historiografia da arquitetura moderna e contemporânea brasileira.This master\'s thesis aims to analyze the spatial transformations of the educational spaces of Municipal Early Childhood Education Schools (EMEI) designed by the team from the Center for Development of Urban and Community Equipment (CEDEC), under the direction of architect Mayumi Watanabe de Souza Lima, between the years 1990 to 1992, in São Paulo, during the mandate of Luiza Erundina (1989 - 1992). This research is primarily located in the broad area of Applied Social Sciences (Architecture, Urbanism and Design) and is characterized by a qualitative approach. For analysis, data obtained through semi-structured interviews, architectural projects, graphic analysis and primary documentation are used. It is therefore proposed to study the design changes that have occurred over the years in schools built with precast mortar technology. With an emphasis on outdoor and play areas, the aim is to investigate the design differences that exist between what currently exists and the original projects designed and carried out by the CEDEC team. This analysis seeks to reflect on the spatial and constructive changes and how its new spatial organization directly reflects on the building and its surroundings. Changes to programs of needs and spaces dedicated to play in the selected projects, their adaptations or, in some cases, suppressions will also be discussed. Ultimately, this work seeks to contribute to studies on the trajectory in teaching and public service and the productions of Watanabe, an author who today occupies a place that is still little highlighted in the historiography of modern and contemporary Brazilian architecture
Functional characterization of a lipoprotein-encoding operon in Campylobacter jejuni
No full textBackground:
Bacterial lipoproteins have important functions in bacterial pathogenesis and physiology. In Campylobacter jejuni, a major foodborne pathogen causing gastroenteritis in humans, the majority of lipoproteins have not been functionally characterized. Previously, we showed by DNA microarray that CmeR, a transcriptional regulator repressing the expression of the multidrug efflux pump CmeABC, modulates the expression of a three-gene operon (cj0089, cj0090, and cj0091) encoding a cluster of lipoproteins in C. jejuni.
Methodology/Principal Findings:
In this work, we characterized the function and regulation of the cj0089-cj0090-cj0091 operon. In contrast to the repression of cmeABC, CmeR activates the expression of the lipoprotein genes and the regulation is confirmed by immunoblotting using anti-Cj0089 and anti-Cj0091 antibodies. Gel mobility shift assay showed that CmeR directly binds to the promoter of the lipoprotein operon, but the binding is much weaker compared with the promoter of cmeABC. Analysis of different cellular fractions indicated that Cj0089 was associated with the inner membrane, while Cj0091 was located on the outer membrane. Inactivation of cj0091, but not cj0089, significantly reduced the adherence of C. jejuni to INT 407 cells in vitro, indicating that Cj0091 has a function in adherence. When inoculated into chickens, the Cj0091 mutant also showed a defect in early colonization of the intestinal tract, suggesting that Cj0091 contributes to Campylobacter colonization in vivo. It was also shown that Cj0091 was produced and immunogenic in chickens that were naturally infected by C. jejuni.
Conclusion/Significance:
These results indicate that the lipoprotein operon is subject to direct regulation by CmeR and that Cj0091 functions as an adhesion mechanism in C. jejuni and contributes to Campylobacter colonization of the intestinal tract in animal hosts.
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Distribution of TT virus (TTV), TTV-like minivirus, and related viruses in humans and nonhuman primates
No full textTT virus (TTV) and TTV-like minivirus (TLMV) are small DNA viruses with single-stranded, closed circular, antisense genomes infecting man. Despite their extreme sequence heterogeneity (>50%), a highly conserved region in the untranslated region (UTR) allows both viruses to be amplified by polymerase chain reaction (PCR). TTV/TLMV infection was detected in 88 of 100 human plasma samples; amplified sequences were differentiated into TTV and TLMV by analysis of melting profiles, showing that both viruses were similarly prevalent. PCR with UTR primers also detected frequent infection with TTV/TLMV-related viruses in a wide range of apes (chimpanzees, gorillas, orangutans, gibbons) and African monkey species (mangabeys, drills, mandrills). These findings support the hypothesis for the co-evolution of TTV-like viruses with their hosts over the period of primate speciation, potentially analogous to the evolution of primate herpesviruses
A study of the sense of loneliness observed in imagination generated by the unconscious: based on a case of clinical psychology practice
No full textA sense of loneliness exists in clients with psychiatric symptoms and psychological problems. Their sense of loneliness is considered linked to both the pathology of their psychiatric disorders -- such as schizophrenia or depression -- and their creation of new world views, such as new insights and discoveries. In this study, the author discusses the human experience of loneliness based on psychotherapy sessions In clinical practice with a client. When people experience true loneliness, they attempt to avoid it by linking to imaginary objects generated by the unconscious, thereby defending themselves from the anxiety and fear caused by loneliness. The imaginary objects, as transitional objects, also have a function in linking the objective and subjective worlds. The author discusses the importance of; (1) 'linking' to internal and external objects just as an embryo is connected to the mother's womb by an umbilical cord, and (2) 'being surrounded' by the environment (i.e., people, Nature) just as an embryo is surrounded in amniotic fluid in the womb, to protect it from destructive loneliness and help in returning to reality from the imaginary world
A Study of Inner Experience in "Clinical Image" : From the Viewpoint of Body Sense in Sandplay
No full textこの論文は国立情報学研究所の学術雑誌公開支援事業により電子化されました。In this study, the image is considered, not from the expressed one or an objective measure, but from the viewpoint of a "Clinical image" where one has "inner experience". The author then focuses on the "body sense", which includes the "sense of touch", felt by those who decided to place items in sandplay. A simplified sandplay task was set as the method: The subjects were asked to decide the place of one item in the state of closed eyes (image situation), and then put the item in the same place actually in the state of open eyes (actual situation). They were then interviewed regarding their experiences. One subjects' report was taken up from among 28 subjects. In the image situation, she told with feeling that she felt the item moved autonomously, the subjective sense. Moreover in the actual situation, it was reported that the sense felt in the image situation changed because of the senses of sight and touch. From these reports, it is assumed that there are two sides in body sense, which exert an influence on the process of sandplay. One is an extremely subjective and undifferentiated sense and the other is the differentiated sense, which includes the senses of touch and sight. In conclusion, in the process of sandplay, the body sense with two sides is interactive
Roman’s Diminutions : How a transcription can help a violinist understand composed ornaments, Johan Helmich Roman’s diminutions over Archangelo Corelli’s Violin sonata no 4 op 5, Adagio 1
No full textThis thesis is a violinists study of diminutions (ornaments; improvisation around an already composed melody) composed by Johann Helmich Roman over the first movement of Archangelo Corelli’s violin sonata no 4 op 5. In order to perform the diminutions the author of this thesis made a transcription of Roman’s handwritten sheet music. This thesis also contains necessary background information for the subject, such as biographies over Roman and Corelli. In the end of the thesis the author is discussing why Roman wrote these diminutions, the author’s thoughts on performing them and if the transcription helped preparing the performance.Repertoire: Archangelo Corelli; Violin sonata no 4 op.5 "Adagio 1", with diminutions by Johan Helmich RomanBaroque violinist in the recording; Torun Fredberg (author)Harpsichordist in the recording; Mayumi Kamata</p
Combining carbon-ion irradiation and PARP inhibitor, Olaparib efficiently kills BRCA1 mutated Triple-negative breast cancer cells
No full text東京女子医科大学博士(医学)博士(医学) 甲第764号(主論文の要旨、要約、審査結果の要旨、本文)，著者名: Miki KAWANISHI, Mayumi FUJITA, Kumiko KARASAWA，タイトル:Combining Carbon-Ion Irradiation and PARP Inhibitor, Olaparib Efficiently Kills BRCA1-Mutated Triple-Negative Breast Cancer Cells，掲載誌:Breast cancer(1178-2234)，巻・頁・年:16巻 p.11782234221080553(2022)，著作権関連情報:© 2022 Author(s).Any re-use terms for users of websites and repositories are restricted to non-commercial and no derivative uses.，DOI:10.1177/11782234221080553doctoral thesi
Combining carbon-ion irradiation and PARP inhibitor, Olaparib efficiently kills BRCA1 mutated Triple-negative breast cancer cells
No full text博士(医学) 甲第764号(主論文の要旨、要約、審査結果の要旨、本文)，著者名: Miki KAWANISHI, Mayumi FUJITA, Kumiko KARASAWA，タイトル:Combining Carbon-Ion Irradiation and PARP Inhibitor, Olaparib Efficiently Kills BRCA1-Mutated Triple-Negative Breast Cancer Cells，掲載誌:Breast cancer(1178-2234)，巻・頁・年:16巻 p.11782234221080553(2022)，著作権関連情報:© 2022 Author(s).Any re-use terms for users of websites and repositories are restricted to non-commercial and no derivative uses.，DOI:10.1177/1178223422108055
A genome-wide gain-of-function analysis of rice genes using the FOX-hunting system
No full textFunding Information: Acknowledgements This work was supported by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Green Technology Project EF-1004). We are grateful to Dr. Takuji Sasaki for his encouragement throughout the project and his excellent advice on the improvement of this manuscript, and to Dr. Shoshi Kikuchi for providing useful information on rice FL-cDNAs. We thank Professors Kokichi Hinata, Atsushi Hirai, Hiroshi Kamada and Masashi Ugaki for their encouragement, critical comments and helpful suggestions, and Drs. Hisato Okuizumi and Hiroyuki Kawahigashi for their administrative support throughout the project. We also thank Mayumi Akagawa, Hiroko Abe, Keiko Mori, Etsuko Sugai, Yumiko Nakane, Ken-ichi Watanabe, Mayumi Takeya, and Kana Miyata for their technical assistance; the members of the Technical Support Section of the National Institute of Agrobiological Sciences for their help in the care of the FOX-rice plants; Haruko Onodera and Kazuko Ono for their technical assistance and advice on rice transformation; Inplanta Innovations Inc. for their technical help on the construction of theThe latest report has estimated the number of rice genes to be ∼32 000. To elucidate the functions of a large population of rice genes and to search efficiently for agriculturally useful genes, we have been taking advantage of the Full-length cDNA Over-eXpresser (FOX) gene-hunting system. This system is very useful for analyzing various gain-of-function phenotypes from large populations of transgenic plants overexpressing cDNAs of interest and others with unknown or important functions. We collected the plasmid DNAs of 13 980 independent full-length cDNA (FL-cDNA) clones to produce a FOX library by placing individual cDNAs under the control of the maize Ubiquitin-1 promoter. The FOX library was transformed into rice by Agrobacterium-mediated high-speed transformation. So far, we have generated approximately 12 000 FOX-rice lines. Genomic PCR analysis indicated that the average number of FL-cDNAs introduced into individual lines was 1.04. Sequencing analysis of the PCR fragments carrying FL-cDNAs from 8615 FOX-rice lines identified FL-cDNAs in 8225 lines, and a database search classified the cDNAs into 5462 independent ones. Approximately 16.6% of FOX-rice lines examined showed altered growth or morphological characteristics. Three super-dwarf mutants overexpressed a novel gibberellin 2-oxidase gene, confirming the importance of this system. We also show here the other morphological alterations caused by individual FL-cDNA expression. These dominant phenotypes should be valuable indicators for gene discovery and functional analysis.Peer reviewe
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