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Preliminary results on ex-vitro conversion of encapsulated somatic embryos of Citrus reticulata Blanco (cv. Mandarino Tardivo di Ciaculli)
Preliminary studies on encapsulation of gametic and somatic embryos of Citrus clementina and Citrus reticulata: effect of cold storage
Le potenzialità delle colture in vitro nella conservazione e diffusione di germoplasma di Morus spp..
Research on conversion of Carrizo citrange [C. sinensis (L.) Osb. × P. trifoliata (L.) Raf.] synthetic seeds.
Perspectives of the encapsulation technology in the nursery activity of Citrus
The genetic and phytosanitary certification has to guarantee the quality of the propagation material also in Citrus. Traditional methods can be assisted by the techniques of in vitro culture, which are fundamental for the mass production of high quality plants. The encapsulation technology represents an innovative tool to combine the advantages of micropropagation with handling ease, storability, reduced size of the propagules, mechanization potentiality, transportability of the gamic seeds. In this work somatic embryos obtained from in vitro anther culture of the cultivar Mandarino Tardivo di Ciaculli (Citrus reticulata Blanco) were encapsulated in a sodium alginate matrix and synthetic seeds were obtained. The conversion ability in non sterile conditions of the synthetic seeds has been evaluated and the addition to the artificial endosperm of the fungicide Thiophanate-methyl did not limit the further development into plantlets of the encapsulated somatic embryos sown on paper (control) and on perlite. A high level of viability has been observed and conversion in ex vitro conditions on substrates similar to those used in the traditional nursery structures (perlite and paper-filter) has been obtained
Estimating alfalfa somaclonal variation in axillary branching propagation and indirect somatic embryogenesis through RAPD fingerprinting.
Le potenzialità delle colture in vitro nella conservazione e diffusione di germoplasma di Morus spp.
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