1,153 research outputs found
Mesotomisation of Diammine=dinitro=oxalo=cobalt Complex and Determination of the Configurations of this Complex and of Diammine=tetranitro=cobalt Complex
No full text1. Diammine-dinitro-oxalo-cobalt complex [Co(NH3)2(NO2)2C2O4]Me has been resolved into the optically active isomers by fractionation of the salts of brucine, strychinine and cinchomine. 2. In the fractionation of the alkaloid salts of the complex, either the l-alkaloid-l-cobalt complex or d-alkaloid-d-cobalt complex was always found to separate out as the least soluble fraction, while either the l-alkaloid-d-cobalt complex or d-alkaloid-l-cobalt complex always contituted the most easily soluble fraction. 3. The specific rotations of potassium and ammonium diammine-dinitro-oxalo-cobaltiates, which have been obtained by replacing the alkaloid molecules with potassium-and ammonium ions respectively, were measured, and found to have the values of ca±115°, using sodium light. 4. As a results of the mesotomisation of diammine-dinitro-oxalo-cobalt complex, the configurations of this complex and also of its mother substance, diammine-tetranitro-cobalt complex, have been made clear, the possibility of mesotomisation indicating that the complex ion [Co(NH2)2(NO2)2C2O4] is constructed asymmetrically, which means that the two ammonia molecules in this complex ion occupy the adjacent spacial position. As to the two nitro-groups, their position was previously determined, by one of the authors (Shibata), by a spectroscopic study. 5. The molecular asymmetry of the above mentioned complex ion, which is the first example of a mesotomised complex anion containing a cobalt atom, belongs to a new type and has been introduced into the stereochemistry of the metal complexes, calling it the type of molecular asymmetry III, in extension of Werner\u27s classification. The senior author, Yuji Shibata, has profound sorrow in recording here the death of his collaborator Mr. Toshio Maruki, Rigakushi, which occurred during the progress of the investigation recorded in this paper. At the same time, he has much pleasure in expressing his hearty thanks to Messrs. K. Matsuno, Rigakushi, and S. Mitsukuri for the great assistance they have given him in completing this work
sj-jpg-2-jhs-10.1177_17531934221150495 - Supplemental material for Comparison of surgical treatments for distal ulna fracture when combined with anterior locking plate fixation of distal radius in the over 70 age group
No full textSupplemental material, sj-jpg-2-jhs-10.1177_17531934221150495 for Comparison of surgical treatments for distal ulna fracture when combined with anterior locking plate fixation of distal radius in the over 70 age group by Ryutaro Shibata, Katsuhiro Tokutake, Yasuhiko Takegami, Tadahiro Natsume, Yuji Matsubara and Shiro Imagama in Journal of Hand Surgery (European Volume)</p
sj-jpg-1-jhs-10.1177_17531934221150495 - Supplemental material for Comparison of surgical treatments for distal ulna fracture when combined with anterior locking plate fixation of distal radius in the over 70 age group
No full textSupplemental material, sj-jpg-1-jhs-10.1177_17531934221150495 for Comparison of surgical treatments for distal ulna fracture when combined with anterior locking plate fixation of distal radius in the over 70 age group by Ryutaro Shibata, Katsuhiro Tokutake, Yasuhiko Takegami, Tadahiro Natsume, Yuji Matsubara and Shiro Imagama in Journal of Hand Surgery (European Volume)</p
sj-jpg-3-jhs-10.1177_17531934221150495 - Supplemental material for Comparison of surgical treatments for distal ulna fracture when combined with anterior locking plate fixation of distal radius in the over 70 age group
No full textSupplemental material, sj-jpg-3-jhs-10.1177_17531934221150495 for Comparison of surgical treatments for distal ulna fracture when combined with anterior locking plate fixation of distal radius in the over 70 age group by Ryutaro Shibata, Katsuhiro Tokutake, Yasuhiko Takegami, Tadahiro Natsume, Yuji Matsubara and Shiro Imagama in Journal of Hand Surgery (European Volume)</p
Long-Term Results of Proton Therapy for Hepatocellular Carcinoma Using Four-Dimensional Computed Tomography Planning without Fiducial Markers
No full text博士論文 要旨Abstract/本文Full 以下に掲載：Cancers 14(23) pp.5842- 2022. MDPI（Multidisciplinary Digital Publishing Institute）. 共著者：Sayuri Bou, Shigeyuki Takamatsu, Sae Matsumoto, Satoko Asahi, Hitoshi Tatebe, Yoshitaka Sato, Mariko Kawamura, Satoshi Shibata, Tamaki Kondou, Yuji Tameshige, Yoshikazu Maeda, Makoto Sasaki, Kazutaka Yamamoto, Hajime Sunagozaka, Hiroyuki Aoyagi, Hiroyasu Tamamura, Satoshi Kobayashi, Toshifumi Gabat
Abstract 4700: Poly G oligonucleotide enhances anti-tumor immune response among the patients with primary lung cancer
No full textAbstract
Synthetic oligonucleotides (ODNs) containing specific sequences have an immunomodulatory effect. It is highly expected that such ODNs can be developed into a novel anti-cancer immunotherapy with these effects. Our past data revealed that a novel poly-G ODN has an anti-tumor immune effect in the tumor-bearing murine model. This effect of poly-G ODN was mediated by T cells in a TLR9 independent manner. Poly-G ODN directly induced the phosphorylation of Lck, thereby enhancing the production of IL-2 and T cell proliferation. Meanwhile, our recent data suggested that the immune effect by poly-G ODN against cancer was different in human. We identified poly-G ODN induced T cell proliferation and production of IFN-gamma through the enhancement of monocyte maturation in human mononuclear cells isolated from peripheral blood or malignant pleural effusion of lung cancer patients. Further studies are continued for the establishment of a novel anti-cancer immune therapy with poly-G ODN.
Citation Format: Nobuaki Kobayashi, Yuji Shibata, Masaki Yamamoto, Takashi Sato, Masaharu Shinkai, Harumi Koizumi, Kenichi Takahashi, Makoto Kudo, Hidekazu Shirota, Takeshi Kaneko. Poly G oligonucleotide enhances anti-tumor immune response among the patients with primary lung cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 4700. doi:10.1158/1538-7445.AM2017-4700</jats:p
Long-Term Results of Proton Therapy for Hepatocellular Carcinoma Using Four-Dimensional Computed Tomography Planning without Fiducial Markers
No full text金沢大学博士(医学）博士論文 要旨Abstract/本文Full 以下に掲載：Cancers 14(23) pp.5842- 2022. MDPI（Multidisciplinary Digital Publishing Institute）. 共著者：Sayuri Bou, Shigeyuki Takamatsu, Sae Matsumoto, Satoko Asahi, Hitoshi Tatebe, Yoshitaka Sato, Mariko Kawamura, Satoshi Shibata, Tamaki Kondou, Yuji Tameshige, Yoshikazu Maeda, Makoto Sasaki, Kazutaka Yamamoto, Hajime Sunagozaka, Hiroyuki Aoyagi, Hiroyasu Tamamura, Satoshi Kobayashi, Toshifumi Gabatadoctoral thesi
From the plate to the brain: associations between dietary patterns and reduced dementia prevalence and white matter lesions in older Japanese adults
No full textPreKURA:1124*Japan Prospective Studies Collaboration for Aging, Dementia (JPSC-AD) study group:
Jun Hata, Mao Shibata, Takanori Honda, Tomoyuki Ohara, Masato Akiyama, Koichi Murashita, Tatsuya Mikami, Songee Jung, Mina Misawa, Naoki Ishizuka, Hiroshi Akasaka, Yasuo Terayama, Hisashi Yonezawa, Junko Takahashi, Masahito Yamada, Kazuo Iwasa, Sohshi Yuki-Nozaki, Shogyoku Bun, Hidehito Niimura, Ryo Shikimoto, Hisashi Kida, Yasuyo Fukada, Hisanori Kowa, Kenji Wada, Masafumi Kishi, Takaaki Mori, Yuta Yoshino, Hideaki Shimizu, Ayumi Tachibana, Shu-ichi Ueno, Tomohisa Ishikawa, Ryuji Fukuhara, Asuka Koyama, Mamoru Hashimoto, Manabu Ikeda, Yoshihiro Kokubo, Midori Esaki, Yuji Takano, Koji Yonemoto, Hisako Yoshida, Kaori Muto, Yusuke Inoue, Yukihide Momozawa, Chikashi Terao, Michiaki Kubo & Yutaka Kiyoharajournal articl
Pure yolk sac tumor of the testis with brain metastasis: report of an adult case
No full textHerein we report an adult case of pure yolk sac tumor with brain metastasis. The patient was a 37-year-old male who presented with indulation of his left scrotum for 10 months. The plain computerized tomographic (CT) scan on entry demonstrated tumor metastasis to his lung and liver and serum alpha-fetoprotein (AFP) level was 786 ng/ml. Five days after admission, he developed hemiplegia secondary to the cerebral metastasis and hemorrhage. After chemotherapy and operation of right-posterior lobectomy, PVB (cisplatinum, vinblastine, bleomycin) chemotherapy produced a complete remission and the elevated serum AFP was normalized. However, the second course of chemotherapy had to be discontinued because of drug-induced hepatitis. He died of massive tumor metastasis to his brain 6 months after craniotomy
Three-dimensional Structure of Nylon Hydrolase and Mechanism of Nylon-6 Hydrolysis
No full textThis research was originally published in the Journal of Biological Chemistry. Seiji Negoro, Naoki Shibata, Yusuke Tanaka, Kengo Yasuhira, Hiroshi Shibata, Haruka Hashimoto, Young-Ho Lee, Shohei Oshima, Ryuji Santa, Shohei Oshima, Kozo Mochiji, Yuji Goto, Takahisa Ikegami, Keisuke Nagai, Dai-ichiro Kato, Masahiro Takeo and Yoshiki Higuchi. Three-dimensional Structure of Nylon Hydrolase and Mechanism of Nylon-6 Hydrolysis. J. Biol. Chem. 2012; 287, 5079-5090. © the American Society for Biochemistry and Molecular BiologyWe performed x-ray crystallographic analyses of the 6-aminohexanoate oligomer hydrolase (NylC) from Agromyces sp. at 2.0 Å-resolution. This enzyme is a member of the N-terminal nucleophile hydrolase superfamily that is responsible for the degradation of the nylon-6 industry byproduct. We observed four identical heterodimers (27 kDa + 9 kDa), which resulted from the autoprocessing of the precursor protein (36 kDa) and which constitute the doughnut-shaped quaternary structure. The catalytic residue of NylC was identified as the N-terminal Thr-267 of the 9-kDa subunit. Furthermore, each heterodimer is folded into a single domain, generating a stacked αββα core structure. Amino acid mutations at subunit interfaces of the tetramer were observed to drastically alter the thermostability of the protein. In particular, four mutations (D122G/H130Y/D36A/E263Q) of wild-type NylC from Arthrobacter sp. (plasmid pOAD2-encoding enzyme), with a heat denaturation temperature of Tm = 52 °C, enhanced the protein thermostability by 36 °C (Tm = 88 °C), whereas a single mutation (G111S or L137A) decreased the stability by ∼10 °C. We examined the enzymatic hydrolysis of nylon-6 by the thermostable NylC mutant. Argon cluster secondary ion mass spectrometry analyses of the reaction products revealed that the major peak of nylon-6 (m/z 10,000–25,000) shifted to a smaller range, producing a new peak corresponding to m/z 1500–3000 after the enzyme treatment at 60 °C. In addition, smaller fragments in the soluble fraction were successively hydrolyzed to dimers and monomers. Based on these data, we propose that NylC should be designated as nylon hydrolase (or nylonase). Three potential uses of NylC for industrial and environmental applications are also discussed
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