1,908 research outputs found
NO RECOGNITION OF MHC CLASS II+ CELLS INFECTED WITH A VACCINIA VIRUS ENCODING INFLUENZA TYPE-A NUCLEOPROTEIN BY CLASS II-RESTRICTED T-CELLS
No full textMHC class II is mostly charged by antigens derived from the outside of the antigen-presenting cell (APC), while class I presents endogenous antigens transported as peptides to the endoplasmic reticulum (ER) by specific transporters. Nevertheless, many antigens, especially glycoproteins, can be presented in vitro by class II even if endogenous. In order to investigate the class-II-restricted T-cell response to endogenously synthesized influenza nucleoprotein (NP) synthesized in infected cells as a model of non-glycosylated nuclear protein, class-II-restricted cytolytic T-cell (CTL) clones were established from BALB/c (H-2d) mice immunized with either influenza A/PR/8/34 (PR8) strain or with a vaccinia virus encoding the NP protein. Two of the clones were characterized in detail and turned out to be cytolytic, I-A(d)-restricted and NP peptide 218-229 specific. Even though an in vivo class-II-restricted T-cell response was elicited in BALB/c mice immunized with a vaccinia virus encoding nucleoprotein (Vacc-NP), class II+ mouse lymphoma cells were not lysed by the class-II-restricted clones in vitro when they were infected with the same virus or with a vaccinia virus encoding a truncated form of NP with no karyophilic sequence, showing that the de novo synthesized protein targeted to the nucleus or remaining in the cytoplasm cannot charge class II through the same pathway as class I in murine APC. These results extend previous observations made on transfected cells to cells that express an antigen during viral infection
APEX DNA Microarray for the Identification of Pathogenic Fungi
No full textThe identification of fungal pathogens, though continuously improving, is still time-consuming and often
inadequate for ensuring an early targeted therapy, which may be crucial for the treatment of invasive mycoses.
Here, we describe a DNA-microarray system based on the arrayed-primer extension (APEX) technique
for a rapid identification of pathogenic fungi, which represents a critical step in medical practice
EFFECT OF ADULT PERITONEAL CELLS ON ANTIBODY RESPONSE OF NEWBORN MICE TO SHEEP RED BLOOD CELLS
No full textThe findings that grafts of adult peritoneal cells enhance the antibody response of newborn mice (1, 2), rats (3) and rabbits (4) have suggested that the immunologic immaturity of newborns might partly be due to a functional deficiency of macrophages.
The results reported here show that unstimulated peritoneal cells (PC), but not peritoneal cells induced by thioglycolate (IPC), are endowed with the property of potentiating the antibody response of newborn mice to sheep red blood cells (SRBC).
Inbred BALB/c mice were used throughout the experiments. PC and IPC were collected from mice of both sexes aged 8 to 12 weeks, the latter cells 3 days after i.p. inoculation of 3 ml thioglycolate medium (Difco Laboratories. Detroit, Mich.), by washing the peritoneal cavity with 5 ml of Eagle's minimal essential medium containing 5 i.u./ml of heparin. Litters of 6 to 8 mice were divided into four groups
Features of Bacillus cereus swarm cells
No full textWhen propagated on solid surfaces, Bacillus cereus can produce differentiated swarm cells under a wide range of growth conditions. This behavioural versatility is ecologically relevant, since it allows this bacterium to adapt swarming to environmental changes. Swarming by B. cereus is medically important: swarm cells are more virulent and particularly prone to invade host tissues. Characterisation of swarming-deficient mutants highlights that flagellar genes as well as genes governing different metabolic pathways are involved in swarm-cell differentiation. In this review, the environmental and genetic requirements for swarming and the role played by swarm cells in the virulence this pathogen exerts will be outlined
Molecular characterization and identification of Bacillus clausii Strains marketed for use in oral bacteriotherapy
No full textA substantial number of Bacillus species have been marketed for use in oral bacteriotherapy because of their purported ability to prevent or treat various gastrointestinal disorders. Recently, some of the Bacillus strains in Enterogermina, which is made up of aqueous suspensions of viable Bacillusspores, have been partially characterized and aligned with members of the Bacillus alcalophilus subgroup rather than withBacillus subtilis, as previously reported. With a view toward verifying the original taxonomic position of the Enterogermina strains, we catalogued both phenotypic and genotypic traits exhibited by the four Bacillus strains isolated from the spore mixtures found in original commercial preparations dated 1975 and 1984 and commercial preparations now being propagated industrially. Analyses of physiological and biochemical traits, complete 16S rRNA gene sequences, DNA-DNA reassociation, tRNA intergenic spacer length polymorphism, single-strand conformation polymorphism of PCR-amplified spacer regions of tRNA genes, and randomly amplified polymorphic DNA led to the finding that all of the Enterogermina strains belong to a unique genospecies, which is unequivocally identified as the alkalitolerant species Bacillus clausii. Moreover, we provide evidence that in contrast to several reference strains ofB. clausii, the strains constituting Enterogermina are characterized by a notable low level of intraspecific genome diversity and that each strain has remained the same for the last 25 years
Pulitzer-Prize Winning Author Sonia Nazario
No full text--Pulitzer-Prize Winning Author Sonia Nazario--Enrique\u27s Journey--Monday, April 11 @ 7 p.m.--Latino Americans 500 Years of History--Sponsors--More information can be found at library.uni.edu/diversityhttps://scholarworks.uni.edu/latinoamericans_documents/1004/thumbnail.jp
Autograph of Sonia Johnson in "The SisterWitch Conspiracy"
No full textThe title page and an autograph by the author, Sonia Johnson, in their work ""The SisterWitch Conspiracy"" with an inscription.To Carolyn, In appreciation of your courage and passion. Soni
Author interview: Q and A with Sonia Livingstone and Alicia Blum-Ross, authors of Parenting for a Digital Future
No full textIn this author interview, we speak to Sonia Livingstone and Alicia Blum-Ross about their new book, Parenting for a Digital Future, which draws on interviews and a national survey with UK parents to explore how hopes and fears about digital technologies are shaping parenting today
Interview with Ruba Salih, by Nadeem Karkabi and Sonia Boulos
No full textOn the occasion of publishing a special issue of Palestine/
Israel Review (PIR) on the decolonization of the city of Haifa, PIR interviewed Ruba Salih, a professor of anthropology at the Department of
Arts, University of Bologna. Salih’s research focuses on transnational
migration and diasporas across Europe, the Middle East, and North
Africa; Islam and gender; the Palestine question and refugees; and
trauma and conflict in the Middle East. She is the author of numerous academic works, including the book Gender in Transnationalism:
Home, Longing and Belonging among Moroccan (Routledge, 2003), and
the article “Bodies That Walk, Bodies That Talk, Bodies That Love: Palestinian Women Refugees, Affectivity, and the Politics of the Ordinary” (Antipode, 2017). Currently, she is working on a book on waiting and the politics of return among Palestinian refugees (Cambridge University Press). Ruba herself is the daughter of a Palestinian refugee from
Haifa, and her mother was born in Yafa/Jaffa. PIR held an interview
with her to talk about her work and her own experience of refuge as a
descendant of Palestinian refugees from Haifa. Nadeem Karkabi and
Sonia Boulos conducted the interview for PIR
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