171 research outputs found
Dataset shifts in medical, cross-silo, federated learning
No full textAuthor Iacopo CiampaMasterarbeit Universität Linz 202
Plasmacytoid urothelial carcinoma of the urinary bladder: Clinicopathologic, immunohistochemical, ultrastructural, and molecular analysis of a case series
No full textA plasmacytoid variant of urothelial carcinoma has been recently recognized in the World Health Organization classification system. This is characterized by a discohesive growth of plasmacytoid cells with eccentric nuclei, extending in the bladder wall and often in the perivesical adipose tissue. Herein, we report the clinicopathologic, immunohistochemical, ultrastructural, and molecular features of a series of plasmacytoid urothelial carcinoma of the urinary bladder. Four bladder carcinomas characterized by epithelial cells with morphologic appearance resembling plasma cells were evaluated at the immunohistochemical, electron microscopic, and molecular genetic levels. Tumor cells stained with cytokeratins, epithelial membrane antigen, GATA-3 (endothelial transcription factor 3), CD15, p53, and p16. In addition, malignant cells strongly stained with CD138 in all the cases, whereas leukocyte common antigen and multiple myeloma 1/interferon regulatory factor 4 were completely negative, nor immunoreactivity was seen for either Îo or λ light chains. The electron microscopic examination showed the presence of divergent squamous and glandular differentiation. At variance with conventional urothelial carcinoma, the analysis of exons 4-9 of TP53 gene revealed no alteration in all the 4 tumors tested, and this can be of value in choosing additional chemotherapy after surgery. Plasmacytoid carcinoma of the bladder is a tumor entity, which can be characterized by specific immunohistochemical markers, including positivity for GATA-3, and presents phenotypic and genotypic peculiarities. © 2011 Elsevier Inc. All rights reserved
Iacopo Sannazaro nella critica d’età barocca
No full textIl saggio si propone di indagare la ricezione di Iacopo Sannazaro nel Seicento, dal punto di vista della sua fortuna critica. A questo scopo, si esaminano le principali opere della prosa critica di età barocca per verificare il ruolo dell’autore dell’Arcadia e della sua poesia italiana e latina nel dibattito letterario contemporaneo.The essay aims to investigate the reception of Iacopo Sannazaro in the 17th century, from the point of view of his critical fortune. To this end, the main works of critical essays from the Baroque period are examined in order to verify the role of the author of Arcadia and his Italian and Latin poetry in the contemporary literary debate
Iacopo da Benevento, giudice e poeta alla corte di Federico II: “status quaestionis”
No full textL’intervento si concentra sui problemi identificativi e cronologici riguardanti la figura storica e letteraria di Iacopo da Benevento, giudice e poeta vissuto in epoca federiciana, autore della commedia elegiaca «De uxore cerdonis» e dei «Carmina moralia», troppo spesso confuso con un altro Iacopo da Benevento, a lui contemporaneo, un frate domenicano autore del «Viridarium consolationis» e di altri scritti di carattere omiletico ed esegetico.The intervention focuses on the identification and chronological problems concerning the historical and literary figure of Iacopo from Benevento, judge and poet who lived in the Frederick era, author of the elegiac comedy «De uxore cerdonis» and of the «Carmina moralia», too often confused with another Iacopo from Benevento, a contemporary of him, a Dominican friar, author of the «Viridarium consolationis» and other writings of a homiletic and exegetical nature
Giuseppe Sardi architetto degli Albani e la chiesa di Sant’Eutizio a Soriano nel Cimino
No full textFigura oggetto di contrastanti giudizi nella storia della critica, qualificato ora come semplice capomastro ora come compiuto architetto, Giuseppe Sardi (1688-1770) ha ricoperto un ruolo rilevante negli sviluppi dell’architettura tardobarocca soprattutto nei primi decenni del Settecento, erede tra i più originali dello sperimentalismo borrominiano ma non estraneo alle nuove istanze di chiarezza neocinquecentesca. E agli esiti più significativi di una diversificata produzione architettonica sembra ora potersi aggiungere la chiesa di S. Eutizio a Soriano nel Cimino, fabbrica patrocinata dalla famiglia Albani durante il regno di papa Clemente XI (1700-1721) che, frutto di un preciso bilanciamento fra le istanze di magnificenza e le contratte possibilità di investimento proprie dell’epoca, introduce nuovi importanti dati di riflessione.Subject to conflicting interpretations, Giuseppe Sardi (1688-1770) played a significant role in the develop- ment of late Baroque architecture, especially in the first decades of the 18th century.
Qualified as both a simple master builder as well as an accomplished architect, surely, he was one of the most original heirs of Borromini’s experimentalism, but not unrelated to the new instances of neo-16th-century clarity. And among his most significant achievements, it seems possible to add now the church of St. Eutizio in Soriano nel Cimino. Spon- sored by the Albani family during the reign of Pope Clement XI (1700-1721), this sacred building seems to be in fact the result of a precise balancing act between the need of magnificence and the reduced investment capacity of that time, introducing unexpected facts to consider
Intracranial mesenchymal chondrosarcoma: Report of two pediatric cases
No full text[No abstract available
Down-regulation of nitric oxide synthase-2 and cyclooxygenase-2 pathways by p53 in squamous cell carcinoma
No full textThe goal of this study was to analyze the correlation between inducible nitric oxide synthase (iNOS) and COX-2 activities and p53 gene status in head and neck squamous cell carcinomas (HNSCCs) in vivo and in vitro. In a series of 43 HNSCCs we observed an up-regulation of both iNOS and COX-2 pathways in tumor tissues and both activities were correlated each other (rs = 0.612 and P = 0.0002). We also found that p53-mutated HNSCCs (25 cases, 58.1%) showed higher levels of iNOS activity and cGMP in comparison with wild-type p53 tumors (18 cases, 41.9%) (P = 0.0005 and P = 0.01), as well as higher iNOS immunohistochemical expression (P = 0.03). Analogously, higher PgE2 levels were documented in p53-mutated HNSCCs when compared with wild-type p53 tumors (P = 0.015) and COX-2 protein expression was higher in p53-mutated HNSCCs (P = 0.007). A431 cancer cells expressing a p53 temperature-sensitive mutant showed an 1.9- and 2.6-fold decrease in spontaneous NO2-/NO3- and PgE2 synthesis at permissive temperature, respectively, when compared with the same cells at nonpermissive temperature (P 0.001). Basal levels of iNOS and COX-2 proteins and mRNAs were markedly suppressed by restoration of p53 activity. Our results indicate that p53 gene mutation(s) may be responsible for iNOS and COX-2 up-regulation frequently observed in HNSCCs and suggest that restoration of wild-type p53 expression may interfere with tumor growth by inhibiting iNOS and COX-2 pathways
Accessit eruditissima Epistola, de ratione edendorum librorum, ad Ioannem Wolfium Tigurinum
No full textIacobo Acontio authore ; Accessit eruditissima Epistola, de ratione edendorum librorum, ad Ioannem Wolfium Tigurinum / eodem author
Cyclooxygenase-2 Pathway Correlates with VEGF Expression in Head and Neck Cancer. Implications for Tumor Angiogenesis and Metastasis
No full textAbstractWe evaluated the role of COX-2 pathway in 35 head and neck cancers (HNCs) by analyzing COX-2 expression and prostaglandin E2 (PGE2) production in relation to tumor angiogenesis and lymph node metastasis. COX-2 activity was also correlated to vascular endothelial growth factor (VEGF) mRNA and protein expression. COX-2 mRNA and protein expression was higher in tumor samples than in normal mucosa. PGE2 levels were higher in the tumor front zone in comparison with tumor core and normal mucosa (P<0001). Specimens from patients with lymph node metastasis exhibited higher COX-2 protein expression (P=.0074), PGEZ levels (P=.0011) and microvessel density (P<.0001) than specimens from patients without metastasis. A significant correlation between COX-2 and tumor vascularization (rs=0.450, P=.007) as well as between COX-2 and microvessel density with VEGF expression in tumor tissues was found (rs=0.450, P=.007; rs=0.620, P=.0001, respectively). The induction of COX-2 mRNA and PGE2 synthesis by EGF and Escherichia coli lipopolysaccharide (LPS) in A-431 and SCC-9 cell lines, resulted in an increase in VEGF mRNA and protein production. Indomethacin and celecoxib reversed the EGF- and LPS-dependent COX-2, VEGF, and PGE2 increases. This study suggests a central role of COX-2 pathway in HNC angiogenesis by modulating VEGF production and indicates that COX-2 inhibitors may be useful in HNC treatment
GENE-03. MICRORNAS PROFILE IN PAEDIATRIC GBMS
No full textMicroRNAs are a novel class of gene regulators and are involved in several physiologic cellular mechanisms, anti-viral defense, cancer and drug sensitivity/resistance of several tumors. Recently, specific microRNA expression profiles have been identified in Glioblastoma Multiforme (GBM), but there are only limited data on the role of microRNA in pediatric GBM (pGBM). In this study we explored the expression profile of 377 microRNAs in 3 pGBM versus a pool of 5 no-tumor pediatric cerebral cortex using TaqMan® Human MicroRNA Array v2.0, Applied Biosystems. We identified a set of microRNAs differentially expressed: miR-490, miR-876-3p and miR-876-5p, miR-448 (under expressed) and miR-501-3p (overexpressed). Through Bioinformatic analysis of this set of miRNAs, we predicted a series of hypothetical target genes: GRIA1, SORL1, NUCKS1, SOX11, SAP30L, HTT, PXMP4, THRB, PSD3, SPN, AGPAT4, USP31, GRIK3, POM121L8P, TNRC6B, SNX29, HIPK2, RIMKLA, ZNF738, LOC388692. We individually validated the expression of all of these genes in 3 pGBM and in 3 cell lines of human GBM (U87MG, A172 and T98G). Interestingly, drug resistant T98G cell line showed an over expression of NUCKS1 (nuclear, casein kinase and cyclin-dependent kinase substrate 1), a cell cycle-related protein that plays an important role in cell proliferation and cell progression. NUCKS1 is overexpressed in many tumors but its precise role in cancer development remains unknown. On the basis of this preliminary report it could be of paramount importance to investigate the role of miR-501-3p and NUCKS1 in the multidrug resistance mechanism of pediatric brain tumors
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