1,720,976 research outputs found
Sanguinarine inhibits VEGF-induced endothelial cell growth in a three dimensional fibrin gel matrix.
No full textQuercitin, a diet-derived flavonoid, acts as a free radical savenger in swine granulosa cell.
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Reactive oxygen species and anti-oxidant defences in swine follicular fluids
No full textA growing body of evidence indicates that the pro-oxidant/anti-oxidant balance inside the ovarian
follicle plays an important role in folliculogenesis. Therefore, the aim of the present study was to
assess the redox status of follicular fluids collected from different-sized swine follicles. We
quantified the most important reactive oxygen species (ROS), namely superoxide anion (O(2)(-)),
hydrogen peroxide and hydroperoxides (ROOH); in addition, we examined the activity of the
detoxifying enzymes superoxide dismutase, catalase (CAT) and glutathione peroxidase and the total
non-enzymatic antioxidant capacity as determined by the ferric-reducing anti-oxidant power assay.
Our data demonstrate that oxidative stress does not affect follicle growth because O(2)(-) levels do
not change during follicle development, whereas concentrations of H2O2 and ROOH are reduced (P
< 0.05). Surprisingly, all non-enzymatic and enzymatic scavengers examined in the present study,
except for CAT, demonstrated reduced activity during follicle development (P < 0.05). Taken
together, these results suggest that other factors could be involved in ROS detoxification during
follicle developmen
Hydrogen peroxide enzymatic detoxifycation in swine follicular fluid: follicular size-dependent changes.
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Sanguinarine inhibits VEGF-induced Akt phosphorylation.
No full textAngiogenesis is the process of vascular growth by sprouting of preexisting vessels. This process
impacts significantly on many important disease states including cancer, diabetic retinopathy, and
arthritis. Endothelial cells receive multiple information from their environment, which leads them to
progress along all stages of new vessel formation. Vascular endothelial growth factor (VEGF), in
particular appears to be a master regulator of this process. This molecule interacts with cellular
receptors and communicates with cell nucleus through a network of intracellular signaling, most of
all by activating Akt pathway. This activation accounts for many of VEGF effects, including cell
survival, migration, tube formation, and promotion of NO release. Sanguinarine (SA), an alkaloid
isolated from Sanguinaria canadensis, is known for its antiangiogenetic effects by suppressing basal
and VEGF-induced new vessel growth. This article was aimed to evaluate the possible effect of SA
(300 nM) on Akt phosphorylation in a porcine aortic endothelial cell line. The alkaloid significantly
(P < 0.001) inhibited the VEGF-induced Akt increase, thus suggesting that this mode of action
could be responsible, at least partially, for the antiangiogenetic effect of SA
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