326 research outputs found
Salvatore Frasca. S. Giustino Martire, Apologie, S. Teojilo Antiocheno, I tre libri ad Autolieo, , 1938
No full textPuech Aimé. Salvatore Frasca. S. Giustino Martire, Apologie, S. Teojilo Antiocheno, I tre libri ad Autolieo, , 1938. In: Journal des savants, Janvier-février 1939. pp. 40-41
Characterization of Neoparamoeba pemaquidensis strains: PCR-RFLP of the internal transcribed spacer region from the amoeba and endosymbiont
No full textNeoparamoeba pemaquidensis continues to be an ongoing problem for commercial finfish aquaculture and has also sporadically been associated with mass mortalities of commercially relevant marine invertebrates. Despite the ubiquity and importance of this amphizoic amoeba, our understanding of the biology as it applies to host range, pathogenicity, tissue tropism, and geographic distribution is severely lacking. This may stem from the inability of current diagnostic tests based on morphology, immunology, and molecular biology to differentiate strains at the subspecies level. In the present study, we developed a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method based on the internal transcribed spacer (ITS) region that can accurately differentiate amoeba strains of N. pemaquidensis. The investigation focused on the complications of the amoeba ITS microheterogeneity in the development of a subspecies marker and the use of the endosymbiont, Ichthyobodo necator related organism (IRO), ITS region as an alternative marker. The combination of host amoeba and endosymbiont ITS PCR-RFLP analyses was successfully used to correctly identify and characterize an N. pemaquidensis isolate from an outbreak of amoebic gill disease in Atlantic salmon Salmo salar from the west coast of North America (Washington State, USA).Charles G. B. Caraguel, Nathanaëlle Donay, Salvatore Frasca Jr., Charles J. O’Kelly, Richard J. Cawthorn Spencer J. Greenwoo
Microheterogeneity and Coevolution: An Examination of rDNA sequence characteristics in Neoparamoeba pemaquidensis and its Prokinetoplastid Endosymbiont
No full textNeoparamoeba pemaquidensis, the etiological agent of amoebic gill disease, has shown surprising sequence variability among different copies of the 18S ribosomal RNA gene within an isolate. This intra-genomic microheterogeneity was confirmed and extended to an analysis of the internal transcribed spacer (ITS) region. High levels of intra-genomic nucleotide diversity (Pi=0.0201–0.0313) were found among sequenced ITS regions from individual host amoeba isolates. In contrast, the ITS region of its endosymbiont revealed significantly lower levels of intra-genomic nucleotide diversity (Pi=0.0028–0.0056) compared with the host N. pemaquidensis. Phylogenetic and ParaFit coevolution analyses involving N. pemaquidensis isolates and their respective endosymbionts confirmed a significant coevolutionary relationship between the two protists. The observation of non-shared microheterogeneity and coevolution emphasizes the complexity of the interactions between N. pemaquidensis and its obligate endosymbiont.Charles G. B. Caraguel, Charles J. O’Kelly, Pierre Legendre, Salvatore Frasca Jr., Rebecca J. Gast, Béatrice M. Després, Richard J. Cawthorn, and Spencer J. Greenwoo
sj-docx-1-vet-10.1177_03009858221133079 – Supplemental material for Ocular lesions of captive cephalopods
No full textSupplemental material, sj-docx-1-vet-10.1177_03009858221133079 for Ocular lesions of captive cephalopods by Kelsey Fiddes, Mike Murray, Salvatore Frasca, Michael M. Garner and Elise E. B. LaDouceur in Veterinary Pathology</p
Lavoro a termine e contrattazione collettiva
No full textNel ripercorrere l’evoluzione della disciplina del lavoro a tempo determinato, considerato come emblema della flessibilità e, al contempo, quale strumento per favorire l’incremento dell’occupazione, l’A. analizza in particolare il ruolo svolto dalla contrattazione collettiva, che talvolta è destinataria di un ampio rinvio legale, talaltra si ritrova ad operare entro ristretti limiti.Analysing the evolution and the regulation of fixed-term contract, considered as a symbol of flexibility and, at the same time, as a means to increase employment, the Author particularly examines the role played by collective bargaining, that sometimes is connected to a large legal referral, sometimes has to operate within stricts limit
Gene expression and pathway bioinformatics analysis detect a potential predictive value of MAP3K8 in thyroid cancer progression
No full text
GALECTIN-3 EXPRESSION MODULATES CHEMORESISTANCE OF ANAPLASTIC THYROID CARCINOMA CELL LINES
No full textPoster # SC12, selected in the short call section
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