1,720,979 research outputs found
DNA extraction from phytoseiid specimens preserved on a glass slide and molecular diagnostic
No full textThis study focuses on the diagnostic of seven species of mite belonging to the family of Phytoseiidae; very important biocontrol agents of phytophagous mites. However, they are morphologically very similar and specific diagnostic is very difficult in fact using the traditional identification methods it is uncertain to identify a species especially when Authors use different characters, very often not stable in the time. In the 60’s M.me Athias-Henriot took into consideration the insemination apparatus that is considered a stable character in the time. According to her, the shape of this apparatus could be considered a major criterion for genus distinction. The aim of the present study is to establish, using molecular investigation, which theories is more correct. DNA were extracted from specimens preserved on a glass slide by three different methods, the organic (phenol-chloroform) Method, the Chelex® 100 extraction procedure and the DNA extraction by a DNneasy Tissue Kit. Phenol-chloroform extraction takes a relatively long period of time (5-6 days), but it produces consistent results. The modified Chelex technique involves less time and work (only 2 hours) and doesn’t require toxic organic solvents, the Qiagen kit did not allow extraction from mites mounted on glass slide 20 years ago. Molecular result (12S rRNA and RAPD-PCR) showed a clear difference between the species
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Mitochondrial DNA deletion and male infertility
No full textMITOCHONDRIAL DNA DELETIONS AND MALE INFERTILITY
Salerno Barbara, Gerardi Eliana and Elena Carra
Dipartimento di Biologia Cellulare e dello Sviluppo,Università di Palermo Viale delle Scienze, Palermo, Italy;
In men, oligozoospermia, asthenozoospermia, teratozoospermia and azoospermia are the main causes of infertility. The present research is aimed to investigate if mtDNA deletions can cause sperm defects in idiopathic astenozoospermic patients with different sperm motility and sperm concentration.
The aim of this investigation was to test the hypothesis that whole sperm samples with lower levels of motility would have a higher incidence of spermatozoa with deletions in mitochondrial genome. We were able to evidence mtDNA when the DNA was amplified from the non motile fraction from semen samples or whether DNA from whole sperm samples was employed long PCR.
In conclusion this study have demonstrated that the use of LPCR clearly indicated mtDNA in patients OAT also with small amount of whole seminal samples. A further indication is that the ND5 and ND6 mtDNA region were preferentially associated to deletions with poor quality semen characteristic
Dal quadro al modello virtuale: una ricostruzione tridimensionale interattiva dell'Albergheria di Palermo nel 1749
Full text linkThis study suggests a virtual three-dimensional reconstruction of a large urban portion of Palermo Historical Centre modelled on an isometrical view of the 17th century entitled Descrizzione del distretto parrocchiale dell’Albergheria fatta l’anno 1749. It is a painting on canvas that has been kept inside the parish of S.Nicolò all’Albergheria, and which in latter days has been taken by the Museum of Palermo Diocese where it is presently exposed to the public.
The work presents different levels of interest for the reading of urban ancient morphology which now has disappeared and for the right information of road textures, blocks and monuments represented there. The digital elaboration that has been made has been overlayed to a second three-dimensional model that has been got from the present maps of Palermo Historical Centre which describe the present configuration of the quarter: thus the model which derives from the 17th century representation has been referred to the real quotes of the ground and to the elevations of the existing buildings which are illustrated and described in the picture. Then all the proposed material has been used to make a new reading of the work and to propose its transformation in a “visual hypertext”. All the elements of the picture have been “translated” as file folders, links about the town history and of its monuments, either disappeared or still existing
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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