253 research outputs found
L' Impostore smascherato. Excerpts - Don Mus.Ms. 1936 : S, orch
No full textGiacomo TrittoQuelle: manuscript, manuscript. - Provenienz: Fürstlich Fürstenbergische Hofbibliothek, Donaueschingen[score:] L'Impostore smascherato - Teatro Nuovo | Dolce amabile nel viso | Aria | Del Sig:re D. Giacomo Tritto | 1794 | F:5 [S:] Aria Del Signore Giacomo Tritt
L’ecocontrastografia venosa nello studio della riperfusione miocardica: quali prospettive nella pratica clinica?
No full textLong-lasting response changes in deep cerebellar nuclei In vivo correlate with low-frequency oscillations
No full textThe deep cerebellar nuclei (DCN) have been suggested to play a critical role in sensorimotor learning and some forms of long-term synaptic plasticity observed in vitro have been proposed as a possible substrate. However, till now it was not clear whether and how DCN neuron responses manifest long-lasting changes in vivo. Here, we have characterized DCN unit responses to tactile stimulation of the facial area in anesthetized mice and evaluated the changes induced by theta-sensory stimulation (TSS), a 4 Hz stimulation pattern that is known to induce plasticity in the cerebellar cortex in vivo. DCN units responded to tactile stimulation generating bursts and pauses, which reflected combinations of excitatory inputs most likely relayed by mossy fiber collaterals, inhibitory inputs relayed by Purkinje cells, and intrinsic rebound firing. Interestingly, initial bursts and pauses were often followed by stimulus-induced oscillations in the peri-stimulus time histograms (PSTH). TSS induced long-lasting changes in DCN unit responses. Spike-related potentiation and suppression (SR-P and SR-S), either in units initiating the response with bursts or pauses, were correlated with stimulus-induced oscillations. Fitting with resonant functions suggested the existence of peaks in the theta-band (burst SR-P at 9 Hz, pause SR-S at 5 Hz). Optogenetic stimulation of the cerebellar cortex altered stimulus-induced oscillations suggesting that Purkinje cells play a critical role in the circuits controlling DCN oscillations and plasticity. This observation complements those reported before on the granular and molecular layers supporting the generation of multiple distributed plasticities in the cerebellum following naturally patterned sensory entrainment. The unique dependency of DCN plasticity on circuit oscillations discloses a potential relationship between cerebellar learning and activity patterns generated in the cerebellar network
Identification of genes involved in RNAi silencing, using crystal-Stellate system in Drosophila melanogaster
No full textExpression and immunolocalization of aquaporin-7 in rat gastrointestinal tract
No full textBackground information. In the gastrointestinal tract of mammals, water can either be secreted with digestive juices or absorbed by the small and large intestine. Transcellular water movement can be mediated by the transmembrane protein family of AQPs (aquaporins), as has also been recently identified in the gastrointestinal tract. However, the localization, expression and functioning of AQPs in the gastrointestinal tract have not been completely characterized. For the present study, we investigated: (1) the expression of AQP7 in some portions of rat gastrointestinal tract by semiquantitative reverse transcriptase-PCR and by immunoblotting and (2) the cellular and subcellular localization of AQP7 by immunohistochemistry.
Results. AQP7 mRNA and proteins were highly expressed in the small intestine, weakly in the caecum, colon and rectum and were absent in the stomach. Immunoblotting analysis using rat gastrointestinal tract membrane fractions showed two major bands corresponding to a molecular mass of approx. 34 and 40 kDa for the AQP7 protein. No bands were observed when the anti-AQP7 antibody was preadsorbed with the immunizing peptide. Immunohistochemistry revealed strong AQP7 labelling in the surface epithelial cells of duodenum, jejunum, ileum, caecum, colon and rectum, whereas weak or no labelling was observed in the crypt cells. The labelling was manifest particularly in the apical membrane but intracellular staining was also observed.
Conclusions. The results indicate that AQP7 is present in the small and large intestine. The higher expression of AQP7 protein at the apical pole of the superficial epithelial cells suggests its involvement in rapid fluid movement through the villus epithelium
Osmotic water permeability of rat intestinal brush border membrane vesicles: involvement of aquaporin-7 and aquaporin-8 and effect of metal ions
No full textWater channels AQP7 and AQP8 may be involved in transcellular water movement in the small intestine. We show that both AQP7 and AQP8 mRNA are expressed in rat small intestine. Immunoblot and immunohistochemistry experiments demonstrate that AQP7 and AQP8 proteins are present in the apical brush border membrane of intestinal epithelial cells. We investigated the effect of several metals and pH on the osmotic water permeability (P-f) of brush border membrane vesicles (BBMVs) and of AQP7 and AQP8 expressed in a cell line. Hg2+, Cu2+, and Zn2+ caused a significant decrease in the BBMV P-f, whereas Ni2+ and Li+ had no effect. AQP8-transfected cells showed a reduction in P-f in the presence of Hg2+ and Cue(+), whereas AQP7-transfected cells were insensitive to all tested metals. The P-f of both BBMVs and cells transfected with AQP7 and AQP8 was not affected by pH changes within the physiological range, and the P-f of BBMVs alone was not affected by phlorizin or amiloride. Our results indicate that AQP7 and AQP8 may play a role in water movement via the apical domain of small intestine epithelial cells. AQP8 may contribute to the water-imbalance-related clinical symptoms apparent after ingestion of high doses of Hg2+ and Cu2+
GENETIC CHARACTERIZATION OF KNOWN AND NOVEL COMPONENTS OF PIRNA PATHWAYS IN DROSOPHILA MELANOGASTER GONADS
No full textMutazioni autosomiche che interferiscono con il sistema crystal-Stellate in Drosophila melanogaster sono risultate alleli di geni attivi nelle prime fasi dello sviluppo
No full textSi tratta di un poster presentato al III Congresso FISV (Federeazione Italiana Scienze della Vita) e scelto per la presentazione orale in un simposio specifico
Driver drowsiness detection relying on infrared thermal imaging: a machine learning approach
No full textOne of the main factors contributing to road accidents all around the world is driver fatigue. For safety reasons, it is essential to identify drowsiness episodes as soon as possible. Numerous studies have shown that PERCLOS, i.e. the percentage of eyelid closure over the pupil across time, is one of the most accurate parameters for drowsiness state assessment. The evaluation of PERCLOS is, however, dependent on the lighting conditions because it is normally computed from the visible video of the subjects. The goal of this study is to get around these constraints by assessing sleepy states using inexpensive, high-resolution thermal infrared technologies. Twelve sleep-deprived participants were chosen for the study, which involved an hour-long driving activity on a driving simulator. Thermal camera Device Alab SmartIr640 was used to capture facial skin temperature throughout the trial, along with facial visible videos of the subjects. Relevant thermal features were estimated from facial regions of interest and extracted over a 30 second time span. A data-driven multivariate machine learning approach based on a three-level Decision Tree Classification of the drowsy state (AWAKE class: PERCLOS0.23) was developed. The average classification accuracy was 0.72±0.05 (mean ± standard deviation). These preliminary results point to the possibility of determining drivers' drowsiness based on facial thermal traits, circumventing the limitations associated with lighting and eye detection that are typical of conventional approaches
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