12,643 research outputs found
Capillipedium mistryi A. P. Tiwari & S. N. Landge 2021, sp. nov.
No full textCapillipedium mistryi A. P. Tiwari & S. N. Landge, sp. nov. (Fig. 1 & 2) Type:— INDIA, Madhya Pradesh, Hoshangabad district, Bori Wildlife Sanctuary, Bori Range, 22°15’ to 22° 30’ N lat. and 77°45’ to 78 30’ E long., ca. 500 m elevation, 15 November 2019, A. P . Tiwari 35 (holotype: CAL!; isotype: BLAT!). Diagnosis:— Capillipedium mistryi is closely allied to C. spicigerum in longer racemes, but differs in pedicel and rhachis internode solid, if slightly channeled then not with a translucent groove (vs. with a distinct translucent groove), shorter panicle 4.0–7.0 × 3.0–5.0 cm (vs. 10–25 × 5.0–8.0 cm in the latter), racemes with 11–25 sessile spikelets (vs. racemes with 3–8 sessile spikelets), sessile spikelet 2.5–3.0 mm long (vs. 3.0–4.0 mm long).Published as part of Tiwari, Arjun Prasad & Landge, Shahid Nawaz, 2021, Capillipedium mistryi (Andropogoneae, Poaceae): a new remarkable species from central India, pp. 51-57 in Phytotaxa 498 (1) on page 52, DOI: 10.11646/phytotaxa.498.1.6, http://zenodo.org/record/542409
Notepad in Java: A notepad made in Java
No full textAn Information Technology Engineering IIIrd Semester mini group project on Notepad a project based on Java language by Smit Shetye, Sarwesh Nandeshwar, Avinash Tiwari at Shivajirao S. Jondhale College Of Engineering. Smit Shetye, Sarwesh Nandeshwar, & Avinash Tiwari. (2021). Notepad in Java (p. 28). Smit Shetye, Sarwesh Nandeshwar, Avinash Tiwari. https://doi.org/10.5281/zenodo.578586
DEVELOPMENT AND CHARACTERIZATION OF A WHOLE GENOME RADIATION HYBRID PANEL FOR TETRAPLOID WHEAT
No full textThe recent release of high-quality sequence information from hexaploid wheat (IWGSC 2014)
coupled with the availability of high-density consensus maps for tetraploid wheat (Maccaferri et al.
2014) has accelerated marker and gene discovery in durum wheat (Triticum durum), thus
facilitating the genetic dissection of agronomic traits (Tuberosa and Pozniak 2014). This
notwithstanding, the construction of genetic maps remains a bottleneck for the investigation of the
durum wheat genome. In this context, the development of physical mapping resources to facilitate
the assembly of BAC contigs in future sequencing projects of durum wheat is becoming a primary
necessity. Radiation hybrid (RH) mapping is a promising recombination-independent mapping
approach, which involves the use of radiation-induced chromosomal breakage and marker
segregation to reconstruct marker order (Tiwari et al. 2016).
In this study, for the first time, a RH panel for tetraploid wheat was developed for reference
durum genotype Svevo (Sv-WGRH panel). The Sv-WGRH panel was developed at Kansas State
University (USA), according to the protocol reported by Tiwari et al. 2016. Freshly dehiscing
pollen of Svevo was irradiated with γ-rays (10-Gy) and this was used to pollinate ~150 emasculated
spikes of Senatore Cappelli (used as the female parent), which produced ~1000 RH1 seeds, each
representing an independent RH event. Greenhouse planting of these 1000 RH1 seeds resulted in
~730 RH1 plants, each representing a RH line of Sv-WGRH panel. Initial assessment of Sv-WGRH
panel was performed based on 19 SSR markers. Results indicated that average marker retention of
Sv-WGRH panel is ~85%. Work is in progress to select most informative 188 RH lines and these
selected lines will be genotyped on a high-density marker platform. This RH panel is an important
resource contributing towards the assembly of the genome sequence of durum wheat and other
tetraploid wheats
Euconocephalus malabaricum Tiwari and Diwakar 2024, sp. n.
No full text<i>Euconocephalus malabaricum</i> Tiwari and Diwakar sp. n. <p>(Figs. 5–6)</p> <p> <b>Material examined:</b> <i>Holotype</i>: Male. <b>INDIA.</b> Kerala, Varnam P.O. Puthanangady, Cherthala, Alleppey. ~ 2 m a.s.l. 2020, Coll. Chandranshu Tiwari, Department of Environmental Studies, University of Delhi, 110007 (Delhi), India. <i>Paratype:</i> Kerala, Varnam P.O. Puthanangady, Cherthala, Alleppey. ~ 2 m a.s.l. 2020, Coll. Chandranshu Tiwari (2 ♂).</p> <p> <b>Type locality:</b> Varnam P.O. Puthanangady, Cherthala, Alleppey Kerela.</p> <p> <b>Measurements:</b> Body 33.1 (1.3); tegmen 40.3 (3.6); fastigium 2.0 (0.4); pronotum 8.1 (0.9), fore-femora 5.7 (0.7); mid-femora 7.3 (0.3); post-femora 20.1 (1.7); fore-tibia 6.1 (0.4); mid-tibia 7.0 (0.2); post-tibia 20.4 (0.3), file 2.6 (0.2).</p> <p> <b>Distribution:</b> Crepescular-Nocturnal. Recorded from rice fields and fallow lands. Likely distributed throughout the Malabar coast.</p> <p> <b>Seasonal occurrence</b>: The species was recorded during the dry season preceding the monsoon.</p> <p> <b>Etymology:</b> The species is named after Malabar coast where it was recorded from. Adjective following <i>Euconocephalus</i> in neuter.</p> <p> <b>Differential diagnosis:</b> The species is similar to <i>Euconocephalus incertus</i> (Walker 1869) but differs in the following character: shorter fastigium, tegmen, and apex of supra-anal plate obtuse. Subgenital plate smaller without the lateral ridges.</p> <p> <b>Description:</b></p> <p> <b>Male:</b> Body slender. <b>Head.</b> Fastigium of vertex conical, elongate 2.0 mm in length, apex rounded. Fastigium of vertex separated from fastigium of frons by a notch with a small ventral tubercle present at the base of fastigium. Eyes suboval and prominent. <b>Pronotum</b> with anterior dorsal margin almost straight, posterior margin broadly rounded; transverse sulcus distinct before the middle of pronotum; lateral carinae present. Lateral lobes of pronotum inclined; longer than high with distinct humeral sinus. Prosternum armed with a pair of spines, mesosternal and metasternal lobes triangular. <b>Legs</b> genicular lobes of fore femora unarmed on both side; genicular lobes of mid femora armed on the inner side with a single spine and unarmed on outer side; genicular lobes of hind femora armed with a single spine on both inner and outer side. Fore coxae armed with a long projected spine.All femora dorsally unarmed. Fore femora ventrally armed with 2 minute spines on inner margin and unarmed on outer margin. Mid femora ventrally unarmed on outer margin and 3 minute spines on inner margin. Hind femora ventrally armed with 9 minute to small spines on inner margin and 7 minute to small spines on outer margin. Tympanum on fore tibia conchate, tympanal slits facing forward with a pair of small elongated pits laterally just below the tympanum. Fore and mid tibiae dorsally unarmed. Fore tibiae ventrally armed with 6 small spines each on inner and outer margin. Mid tibiae ventrally armed with 7 small spines each on inner and outer margin. Hind tibiae dorsally armed with 15 small sized spines on inner and 19 spines on outer margin. Hind tibiae ventrally armed with 9 small spines on inner margin and 17 small more spaced spines on outer margin. Single dorsal pair of spurs and two ventral pairs (inner small and outer large) of spurs present at the apical region of hind tibiae. Male tenth abdominal tergite with two incurved lobes on apical margin. <b>Wings.</b> Tegmina longer than hind wings but not extending beyond the hind wings; apices obtusely rounded. Wings reaching beyond the middle of hind tibia when folded. Hind wings pellucid. <b>Stridulatory file</b> with 76 teeth on the ventral side of left tegmen; file slightly curved. relatively narrow on distal end than the proximal end.</p> <p> <b>Male genitalia.</b> Supra-anal plate triangular with dorsal basal groove, apex obtuse. Subgenital plate broad with well developed medial ridge. Apical margin with triangular excision; styles conical and short. Cerci thick with two incurved apical teeth, dorsal tooth shorter than ventral tooth.</p> <p> <b>Female</b>. Unknown. Supposedly similar to <i>E.incertus.</i></p> <p> <b>Coloration</b>. Green to Yellow Green when alive. Antennae yellow to pale green. Lateral margin of fastigium of vertex on both sides with narrow yellow band which extends on the vertex above eye level and reaching up to the posterior margin of pronotum. Tegmen appears green/yellow when live with anterior external margin of the tegmen pale or translucent. All femora same color as tegmen. Tibia pale yellow to white. Claws and tarsal joints pale.</p> <p> <b>Depositories:</b> The specimens are deposited in the Department of Environmental Studies, Faculty of Science, University of Delhi.</p> <p> <b>Acoustic Description</b></p> <p> The call of <i>E. malabaricum</i> consists of a buzzing call typical to Conocephalinae family, with a two part echeme (SD=4±1 ms) produced at a very high rate (209±37 echeme/s). The call had the peak frequency of 12 kHz and a bandwidth of 7±1.5 kHz (Figure 6).</p>Published as part of <i>Tiwari, Chandranshu & Diwakar, Swati, 2024, The Orchestra Nocturne: Description For Six New Katydid Species From India (Orthoptera: Tettigoniidae), pp. 227-245 in Zootaxa 5405 (2)</i> on pages 237-239, DOI: 10.11646/zootaxa.5405.2.4, <a href="http://zenodo.org/record/10603343">http://zenodo.org/record/10603343</a>
RTA 2012 Proceedings Frontmatter
No full textFrontmatter, Table of Contents, Conference Organization, External Reviewers, Author Index
Transcriptional control of epithelial to mesenchymal transition by regulatory factors and epigenetic mechanisms
No full textThe World Health Organization (WHO) states cancer to be a leading cause of death worldwide accounting for 7.6 million deaths (around 13% of all deaths) and is projected rising to over 11 million in 2030. This is an alarming call to researchers for putting more effort into the analysis of the underlying patho-mechanisms. In a very simplified manner, cancer represents the destruction of healthy tissues and organs by uncontrolled cell proliferation and subsequent formation of a tumor. One key feature of solid tumors that marks the mostly deadly feature of the disease is the acquisition of the potential to invade into the surrounding tissue and form secondary tumors at distant sites, a process called ‘metastasis’. To gain migratory and invasive properties, cancer cells undergo epithelial to mesenchymal transition (EMT) where epithelial cells lose epithelial properties, e.g. their polarized organization and cell-cell junctions, and thus undergo changes in cytoskeleton organization and cell shape and acquire mesenchymal characteristics. Importantly, besides the formation of metastatic lesions, EMT is also involved during development as well as wound healing.
To gain insights into the complex process of EMT and to identify new potential markers for ongoing metastasis, we established different in vitro EMT model systems. Global expression profiling during TGF-β-induced EMT revealed genome-wide transcriptome reprogramming during EMT and identified Krupple-like factor 4 (Klf4) and the SRY-Related HMG-Box Gene4 (Sox4) as one of the key transcription factors that were modulated and may possibly contribute to transcriptional changes during EMT.
We investigated the role of Klf4 and Sox4 during EMT by employing two different in vitro systems of EMT, using normal murine mammary gland (NMuMG) and Polyoma middle T- breast cancer (Py2T) cells, which undergo a progressive EMT upon transforming growth factor (TGF-β) treatment. We further validated the role of Sox4 in breast cancer carcinogenesis in vivo by orthotropic injection of Sox4-depleted cells into the mammary fat pad of nude mice. In addition, we also investigated whether such TGF-β-induced EMT accompanies epigenetic reprogramming and revealed how Polycomb group (PcG) complex-mediated H3K27me3 modification modulates transcription of key genes underlying this process, thereby regulating EMT.
Klf4 is a zinc-finger protein, known to be abnormally expressed in various cancers. It can act as a tumor suppressor or as an oncogene in context dependent manner in different carcinomas. Klf4 is downregulated during TGF-β-induced EMT. Our data reveal a tumor suppressor role for Klf4 in breast carcinogenesis. Klf4 is essential for the maintenance of an epithelial phenotype during EMT, and forced expression of Klf4 leads to blockage of epithelial differentiation. Furthermore, Klf4 is inhibitory to EMT-driven cell migration and also behaves as a survival factor during TGF-β-induced EMT. Genome-wide location analysis by next generation ChIP-seq analysis revealed that Klf4 directly occupies the promoter of many key EMT genes such as N-cadherin, Vimentin, β-catenin and Mapk8. Moreover, one of these Klf4 targets, Mapk8, encoding Jnk1, is upregulated during EMT and a double-knockdown of Klf4 and Jnk1 is able to overcome Klf4 knockdown-induced EMT, migration and apoptosis. These observations underscore a role of Klf4 during EMT by targeting and regulating crucial EMT genes.
Sox4 is also known to be deregulated in many cancers. Sox4 is upregulated during TGF-β-induced EMT. We show that Sox4 is required for maintaining mesenchymal identity and depletion of Sox4 prevents TGF-β-induced EMT. Sox4 reduction further impairs the migratory capacity of cells. Moreover, Sox4 provides a survival advantage to cells during breast carcinogenesis. In addition, Sox4 contributes towards TGF-β-induced tumorigenicity and metastatic spread. Gene expression profiling after Sox4 depletion in complementation with Chromatin immunoprecipitation analysis revealed many key EMT genes such as Spred1, Edn1, Palld, Cyr61, Ereg, Areg and Yap1 which are directly targeted by Sox4 for transcriptional regulation. Furthermore, Sox4 also controls many genes which are shown to regulate various other features of EMT as well as cancer development such as angiogenesis, adhesion, migration, morphogenesis, cell cycle and cytoskeleton re-modeling. Ezh2, a catalytic subunit of the Polycomb Repressive Complex 2 (PRC2), has been also found to be transcriptionally regulated by Sox4. To delineate the role of Ezh2 during EMT, a loss of function approach has been used to demonstrate that Ezh2 is required for proper acquisition of EMT and EMT-driven processes such as migration and apoptosis. Taken together, our data provides a role of Sox4 during EMT via transcriptional regulation of key genes, including the Polycomb component, Ezh2.
We also studied the role of two prominent epigenetic modifications- DNA methylation and histone 3 lysine 27 tri-methylation (H3K27me3) during TGF-β-induced EMT in a mammary epithelial cell line. Our data revealed no evidence of a reprogramming of DNA methylation during this process. To assess the role of H3K27me3 during EMT, we performed chromatin immunoprecipitation using H3K27me3-specific antibodies followed by next-generation sequencing (ChIP-seq) on 6 different stages of EMT progression. This analysis revealed that many key EMT genes are regulated by H3K27me3 mark including Mcam, Pdgfrb and Itga5 which are repressed by this mark in epithelial cells and loose it during EMT as they get activated conversely, Cdh1, Ocln and Cdx2 gain this mark during EMT and get repressed in mesenchymal cells. We further illustrated that the coordinated activities of Ezh1 and Ezh2 are required for H3K27me3-mediated repression of the gene expression and their co-depletion de-represses target genes and blocks EMT. This study provides novel insights into the important regulatory role of the Polycomb machinery during EMT.
In summary, our findings demonstrate how transcription factors, such as Klf4 and Sox4 and the epigenetic machinery, such as PcG proteins, regulate EMT by directly contributing to the transcriptional reprogramming underlying this process
Figs 11–15. Myrmecina urbanii Tiwari. 11 in TWO NEW SPECIES OF THE GENUS MYRMECINA CURTIS, 1829 (HYMENOPTERA: FORMICIDAE: MYRMICINAE) FROM INDIA
No full textFigs 11–15. Myrmecina urbanii Tiwari. 11 – head in full-face view; 12 – body in profilePublished as part of Sheela, S., Kazmi, S. I. & Roy, S., 2020, TWO NEW SPECIES OF THE GENUS MYRMECINA CURTIS, 1829 (HYMENOPTERA: FORMICIDAE: MYRMICINAE) FROM INDIA, pp. 13-19 in Far Eastern Entomologist 403 on page 17, DOI: 10.25221/fee.403.2, http://zenodo.org/record/716536
Capabilities of proprietary intermediate telehealth devices
No full textAbstract Objective: Proprietary intermediate telehealth devices are those which
are specifically designed as connectors between the entities of telehealth
systems. This article seeks to understand what are the capabilities of such
devices and then to investigate how these are clustered on the current
generation of devices. Materials and Methods: Fourteen current-generation
devices available from 12 device providers were selected and analyzed. Four
categories of questions were composed to evaluate the devices: setup/
configuration, available features, inputs, and outputs. Results: Data were
collected and synthesized on the following capabilities: availability from
suppliers, setup, environments of use, multiple-condition monitoring, multiuser
capabilities, prompts, reminders and alerts, interaction with the health
professional, access to historical data, device inputs, and their transfer
technology. Conclusions: There are three main roles for proprietary intermediate
devices in telehealth systems: displaying information to the patient; receiving
data manually/automatically; forwarding results and questionnaire responses to
another entity. Provider Perspective: Intermediate devices are usually part of
closed proprietary systems. Providers produce disease-customisable devices.
Connectivity is considerably ahead of the current generation of point-of-care
devices. However, little data are available on connection to rest of the
proprietary system. Patient Perspective: It shows clear benefit that one
intermediate device can be potentially used with several chronic conditions.
Simple setup, authentication procedures, and automatic data transfer are key
design aspects. Health Professional Perspective: Little direct interaction with
the health professional was observed. Payer Perspective: Details of costs of
devices are generally unavailable; system providers indicate that cost
variability is based on "user requirements.
The emergence of Indian multinationals: An empirical study of motives, status-quo and trends of Indian investments in Germany
No full textGermany has advanced to the position of a key destination for Indian multinational enterprises in their spirited pursuit of growth opportunities overseas. In 2008, Indian firms invested an estimated amount of US$ 1.8 billion in Germany while 16 acquisitions by Indian firms were monitored, up from 7 in 2007. In fact, Indian FDI stock in Germany seems to have surpassed German FDI stock in India. Our research shows that, as of October 2008, 123 Indian MNEs with 167 subsidiaries were active in Germany and had approx. 20,000 full-time employees on their pay-rolls. However, there has been hardly any independent, academic research, so far, on Indian investments in Germany, especially on the motives, experiences, and employment effects on the host and home economies. The present study, presumably the only empirical study of Indian firms overseas to date, provides unique insights into the motives, operations, experiences, and future plans of Indian firms. It shows that Indian firms have generally performed well and intend to further strengthen their operations, including in research & development activities, in Germany. Nonetheless, firms are also faced with several challenges, including but certainly not limited to cross-cultural issues, which need to be mastered. There are also sectoral differences in the motives, experiences, and location selection criteria of Indian MNEs. --Globalization,Multinational Enterprises,Foreign Direct Investments,Mergers & Acquisitions,India
Mobile services in banking sector: The role of innovative business solutions in generating competitive advantage
No full textThe wide-ranging economic developments of the previous decade, e.g. the integration of world economies, have made a significant impact towards increasing the mobility of the working populace and their families. At the same time, technological developments especially in the field of telecommunication have made it possible to offer innovative, location sensitive services on ubiquitous basis to customers on the move. Our paper examines innovative mobile solutions in the field of mobile financial services (MFS) by using four case studies from Germany and Switzerland - representing two banks and two different technology solutions. The paper scrutinizes the strategic relevance of MFS to the competitive position of the firm concerned. Finally, we present five propositions about the role of innovative business solutions in the banking sectors and recommend that a large scale empirical study to test these propositions be conducted in the future. --Mobile Banking,Mobile Commerce,Mobile Financial Services,Multi-channel strategy,Innovation in banking sector
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