1,720,975 research outputs found
The effect of pH on the calcium ionophore A23187 induced acrosome reaction in boar semen : a preliminary study
No full textAspetti tecnici del prelievo ecoguidato transvaginale di ovociti bovini
No full textNella specie bovina con la messa a punto di tecniche per la produzione di embrioni “in vitro” si è determinata l’esigenza di reperire ovociti in numero cospicuo e nel modo più agevole possibile per sviluppare programmi di riproduzione e di selezione basati sulle tecniche “in vitro”. Nel presente lavoro vengono descritte tre diverse versioni di aghi cosiddetti semimonouso e i risultati del loro impiego in termini di risultati di percentuali di raccolta di ovociti rispetto ai follicoli aspirati e di qualità degli ovociti ottenuti(cumulo ooforo intatto, parzialmente intatto, assente ) I migliori risultati(82,7% di recupero e 76,4% di ovociti di buona qualità ) sono stati ottenuti impiegando aghi monouso per anestesia epidurale (Terumo 18G) opportunamente modificati e connessi ad un tubo prolunga in acciaio (diam. int. 2 mm) con la possibilità di estrazione dal portasonda per eseguire un lavaggio dopo la puntura-aspirazione di ciaschedun follicolo.In this work are described three different disposable needles to be used in bovine ovum pick- up. The best results, 82.7% of collection rate (123 oocytes collected out of 154 follicles diam. ≥4 mm aspirated ) with 76.4% of cumulus intact oocytes were obtained using disposable Terumo 18G spinal needles customized to be attached to a stainless steel tube ( length 60 cm, internal diam. 2 mm) The main features of this long semi –disposable needles are that they are both inexpensive and with the advantage of a very sharp point at every ovum pick-up(OPU) session
Preliminary evaluation of Carbofuran Cytotoxicity and steroidogenesis effects on Bovine Granulosa cells
No full textIncubazione in liquido follicolare preovulatorio di spermatozoi di stallone freschi e crioconservati
No full textRecently, several studies were conducted to develop new artificial insemination techniques in horses based on reduced spermatozoa dose. Few data are available on intrafollicular insemination in mare. We analyzed morfological and functional changes of fresh and cryopreserved stallion spermatozoa incubated for six hours in preovulatory follicular fluid at 1:2 and 1:50 concentrations. Fresh sperm viability decreased from 78% to 57% at the dilution 1:2, whereas no changes were recorded for frozen spermatozoa (38% to 39%). At diluition 1:50 sperm viability showed a similar trend and there were no statistical differences with dilution 1:2. Either fresh and cryopreserved sperm showed a slight increase in acrosomal reactions during incubation at 1:2 dilution. At 1:50 dilution acrosome reactions in thawed sperm didn’t change significantly (25.86% to 18.42%), while for fresh sperm the variation from 22.58% to 51.28% risulted to be significant (P<0,05)
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Caratteristiche dello sperma in rapporto alla stagionalità dell'attivita’ sessuale della specie bufalina
No full textApoptosis and mitosis in pre antral porcine follicles during short term culture in a biocompatible matrix
No full text
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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