18,445 research outputs found
S. Prakash Sinha, New Nations and the Law of Nations
No full textS. Prakash Sinha, New Nations and the Law of Nations. In: Revue internationale de droit comparé. Vol. 21 N°2, Avril-juin 1969. pp. 454-456
S. Prakash Sinha, New Nations and the Law of Nations
No full textS. Prakash Sinha, New Nations and the Law of Nations. In: Revue internationale de droit comparé. Vol. 21 N°2, Avril-juin 1969. pp. 454-456
Investigations of Streptomyces coelicolor A3(2) siderophore binding proteins
No full textSiderophores are small, high-affinity ferric iron chelators released by many microorganisms and some plants to solubilize iron. They are of great interest due to their clinical use to treat iron overload in humans, and also in relation to the development of novel antibiotics that target the biosynthetic and uptake pathways for iron in pathogens. Pathogens such as Bacillus anthracis excrete more than one type of siderophore. This is linked to increased pathogenicity. The Gram-positive soil bacterium Streptomyces coelicolor A3(2) excretes three siderophores: desferrioxamine B, desferrioxamine E and coelichelin. These displace iron from insoluble ferric hydroxides, and the resulting ferric complexes are transported into the cell via siderophore-binding proteins (lipoprotein receptors) associated with ATP-binding cassette (ABC) transporters. Previous studies showed that some of the genes in the biosynthetic clusters of the desferrioxamines (des) and coelichelin (cch) were required for efficient uptake of ferrioxamine E and ferri-coelichelin respectively and a third ABC transporter gene cluster (cdt), not associated with siderophore biosynthesis genes, was implicated in the import of ferrioxamine B. In this study, the lipoprotein receptors encoded within the des, cch and cdt clusters - DesE, CchF and CdtB – were recombinantly overproduced in E. coli and purified by immobilized metal affinity chromatography. Also, ferri-coelichelin was purified from cultures of S. coelicolor. The binding of the ferric complexes of the three cognate siderophores, as well as the xenosiderophores ferrichrome and ferrialbomycin, to the lipoprotein receptors was monitored by intrinsic fluorescence quenching. Dissociation constants of receptor-siderophore complexes were found to be in the nanomolar range, and a revised model of cognate siderophore transport in S. coelicolor was proposed. In collaboration with researchers at St. Andrews University, an X-ray crystal structure was solved for apo-DesE and DesE bound to ferrioxamine B, which demonstrated the similarity of DesE to other bacterial siderophore-binding proteins and the negligible conformational change on substrate binding. Ferrioxamine B also exhibited an unusual configuration not observed before in X-ray crystals of this ferri-siderophore. Also, a forcefield was constructed to model the structure and distortions ferric-tris-hydroxamate complexes, which could be used in the future to investigate the molecular basis of the tight and specific binding of ferri-siderophores to siderophore-binding proteins
Notes on some crinoid associated decapod crustaceans (Crustacea: Decapoda) of Lakshadweep Archipelago, Central Indian Ocean
No full textPrakash, S., Marimuthu, N. (2020): Notes on some crinoid associated decapod crustaceans (Crustacea: Decapoda) of Lakshadweep Archipelago, Central Indian Ocean. Zootaxa 4766 (1): 86-100, DOI: 10.11646/zootaxa.4766.1.
Body on the Barricades is a book of hope amidst curtailment of rights and freedom
No full textK. Kalyani interviews Brahma Prakash, author and academician, on his new book, Body on the Barricades: Life, Art and Resistance in Contemporary Indi
The Folio: Forman Christian College Magazine
No full textIqbal Number [Urdu Section]Satyindra Singh-Editorial. pp. 1-2; Latif, I.-Essay-The Unconscious Mind. pp. 3-7; Story-The World of Darkness. pp. 7-10; Prakash Chandar Chopra-A Student Looks at Television. pp. 10-11; Basant Saigal-News and Notes. pp. 12-14; Malik, H.-Spring. pp. 14-15; Trilokinath Anand-The Average Graduate. pp. 15-17; Satyindra Singh-Sports. pp. 17-18; The Co-Eds' Corner. pp. 18; Mathai, S.-Essay-Shakespeare's Comedies of the Middle Period. pp. 18-22; Hostel News. pp. 22-25; Satyindra Singh-Societies. pp. 25-26; Om Prakash Sharma-Poetry-Dark Light. pp. 26; Alumni Corner. pp. 27; W. P. B. pp. 29-30; Late News. pp. 30-31; Obituary. pp. 31; [Hindi]. 17 p.; Folio [Urdu]. 16 p.Editorial Staff. before page
Kinship genomics approach to study mating systems in a depleted sea turtle rookery
No full textKnowledge of demographic processes and life history strategies is necessary for the conservation and management of endangered sea turtle populations, but it is difficult to ascertain because of the limited accessibility to marine environments that sea turtles use during the different stages of their life cycle. In such cases, molecular genetics and genomic approaches are useful to assess mating systems and operational sex ratios (OSR), which ultimately influence demography. This pilot study used genome-wide single nucleotide polymorphism (SNP) genetic markers for exploring kinship and mating systems in sea turtles where major obstacles prevent a comprehensive assessment in the wild. We sampled 217 young hawksbill turtles (Eretmochelys imbricata) of unknown parentage that had originally been collected locally from seven nests and were being temporarily kept in captivity by Treasure Island Ltd as part of its captive rearing conservation project at Bounty and Treasure Islands, Fiji, in the South Pacific. The raw dataset comprised 13,573 SNPs, of which we retained 639 SNPs for parentage and relatedness analyses. Our findings from seven different pairs of parents suggest a 1:1 male:female OSR and demonstrate that genome-wide SNP genotyping approaches can be used to infer OSR. Knowledge of OSR can help evaluate the magnitude of the impact of warming temperatures and consequent feminisation in sea turtles. Our approach can complement or substitute field observation of breeding males and nesting females when logistical or budgetary constraints prevent observation of OSR in wild sea turtle populations. This approach allows inference of OSR. Protection of beaches with a higher number of pairs of parents should be prioritised to increase genetic resilience. Conservation actions in rookeries with a female-skewed OSR should be prioritised to address population declines in the long run.The research was supported by The University of the South Pacific (USP SRT grant F1006-RI001-71502-623 to S.P. and C.R. and USP FRC grant F3086-FST15-71502-001 to S.S.P.).Peer reviewe
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