89 research outputs found
Poloczek Jarrold rehearsal multi method
No full textData associated with a paper authored by Poloczek, S., & Jarrold, C. The Development of the Adaptive Use of Different Forms of Rehearsal in Verbal Serial Recall Tasks. A Multi-Method Stud
Women’s Power To Be Loud: The Authority of the Discourse and Authority of the Text in Mary Dorcey’s Irish Lesbian Poetic Manifesto “Come Quietly or the Neighbours Will Hear”
No full textThe following article aims to examine Mary Dorcey’s poem “Come Quietly or the Neighbours Will Hear,” included in the 1991 volume Moving into the Space Cleared by Our Mothers. Apart from being a well-known and critically acclaimed Irish poet and fiction writer, the author of the poem has been, from its beginnings, actively involved in lesbian rights movement. Dorcey’s poem “Come Quietly or the Neighbours Will Hear” is to be construed from a perspective of lesbian and feminist discourse, as well as a cultural, sociological and political context in which it was created. While analysing the poem, the emphasis is being paid to the intertwining of various ideological and subversive assumptions (dominant and the implied ones), their competing for importance and asserting authority over one another, in line with, and sometimes, against the grain of the textual framework. In other words, Dorcey’s poem introduces a multilayered framework that draws heavily on various sources: the popular culture idiom, religious discourse (the references to the Virgin Mary and the biblical annunciation imagery), the text even employs, in some parts, crime and legal jargon, but, above all, it relies upon sensuous lesbian experience where desire and respect for the other woman opens the emancipating space allowing for redefining of one’s personal and textual location. As a result of such a multifarious interaction, unrepresented and unacknowledged Irish women’s standpoints may come to the surface and become articulated, disrupting their enforced muteness that the controlling heteronormative discourse has attempted to ensure. In Dorcey’s poem, the operating metaphor of women’s silence (or rather – silencing women), conceived of, at first, as the need to conceal one’s sexual (lesbian) identity in fear of social ostracism and contempt of the “neighbours,” is further equated with the noiseless, solitary and violent death of the anonymous woman, the finding of whose body was reported on the news. In both cases, the unwanted Irish women’s voices of either agony, during the unregistered by anybody misogynist bloodshed that took place inside the flat, or the forbidden sounds of lesbian sexual excitement, need to be (self) censored and stifled, not to disrupt an idealised image of the well-established family and heteronormative patterns. In the light of the aforementioned parallel, empowered by the shared bodily and emotional closeness with her female lover, and already bitterly aware that silence in discourse is synonymous with textual, or even, actual death, the speaker in “Come Quietly or the Neighbours Will Hear” comes to claim her own agency and makes her voice heard by others and taken into account
Analýza trhu vysokorychlostního připojení k internetu v ČR
No full textCílem této práce je poskytnout čtenáři náhled na situaci vysokorychlostního připojení k Internetu v České republice. Na úvod je představena historie internetu a jsou popsány nejpoužívanější technologie k vysokorychlostnímu připojení k internetu. Následuje porovnání poptávky a nabídky na trhu vysokorychlostního připojení v České republice. Nebere se v potaz pouze technická stránka, ale též ekonomická situace, vládní regulace a změny od roku 2009. V závěru práce je shrnutí a vyhodnocení výsledků spolu s nastíněním budoucího vývoje trhu s vysokorychlostním připojením v České republice.The objective of the thesis is to provide a view of the situation in high-speed Internet connection in the Czech Republic. At the beginning we present a history of internet and describes in particular the most frequently used technology of high-speed connectivity to the Internet. This is followed by a comparison of the current supply and demand in a market of Internet connection in the Czech Republic. This study not only compare technological aspects, but takes into consideration the economic situation, government regulations and changes from 2009 as well. Finally, there is a summarization and an evaluation of the results and a reflection on possible future on a market of high-speed connection in Czech republic
Is Perceptual Priming Affected by Culture? A Study With German Middle-Class and Cameroonian Nso Farmer Children
No full textVöhringer IA, Poloczek S, Graf F, et al. Is Perceptual Priming Affected by Culture? A Study With German Middle-Class and Cameroonian Nso Farmer Children. The Journal of Genetic Psychology. 2015;176(3):156-170.The authors explored priming in children from different cultural environments with the aim to provide further evidence for the robustness of the priming effect. Perceptual priming was assessed by a picture fragment completion task in 3-year-old German middle-class and Cameroonian Nso farmer children. As expected, 3-year-olds from both highly diverging cultural contexts under study showed a priming effect, and, moreover, the effect was of comparable size in both cultural contexts. Hence, the children profited similarly from priming, which was supported by the nonsignificant interaction between cultural background and identification performance as well as the analysis of absolute difference scores. However, a culture-specific difference regarding the level of picture identification was found in that German middle-class children identified target as well as control pictures with less perceptual information than children in the Nso sample. Explanations for the cross-cultural demonstration of the priming effect as well as for the culturally diverging levels on which priming occurs are discussed
Index for Commitment to inclusion
No full textIn the Italian context the children with special educational needs and disabilities are included in mainstream schools since the Italian legislation had recognised the importance of the process of inclusion to the activity and participation dimensions of the child and the importance of the environmental factors to the determination of a disability.
Starting from the “Index for inclusion” (Booth et al., 2000) the purpose of this study is to modify this document by simplifying and compressing the items and to create an instrument that can be more easy to use. The new “Index for Commitment to inclusion” can be used by the schools in a double way:
- to recognise specific actions for inclusion in which schools are committed to, and which could be observed, implemented and evaluated;
- to certificate and accredit their process of inclusion.
In the first phase of the study we have created three tools to which schools, students and parents/carers give their responses (based on a 6-point scale) considering the process to inclusion of their school. A representative group of schools (primary and secondary schools) of the Veneto Region spread over all the different provinces is involved, in collaboration with the Regional Instructional Office and an on line form of the tools is provided. The second phase of the research will extend the participation to all Veneto Schools. The implications of these findings are considerable because the study provides results directly derived from the application of a Commitment version of Index in which the corresponding actions for inclusion made by teachers and schools politics in that direction are expressed and became sharable and available among different institutions and evaluable also by families and students. The data implementation is still in progress and will be presented and discussed in the paper, as well as the main educational implications of the results
Biochemical Characterization of Escherichia coli PgaB, an Enzyme Essential for Biofilm Formation
No full textThe formation of bacterial biofilms requires an extracellular matrix to facilitate adherence of bacteria to the surface they colonize. Carbohydrate polymers, known as exopolysaccharides, form a key component of most biofilm matrices. A wide variety of medically-important biofilm forming bacterial strains, including S. epidermidis, S. aureus, E. coli, B. pertussis, and Y. pestis generate the same β-1,6-N-acetyl glucosamine (PNAG) homopolymer as a key biofilm matrix exopolysaccharide. In E. coli, as well as in the other bacterial strains, the PNAG undergoes partial enzymatic de-N-acetylation, which is essential for surface attachment and subsequent biofilm formation. In vivo studies implied that the enzyme responsible for carrying out de-N-acetylation in E. coli is PgaB, an enzyme with sequence homologues in many Gram negative species capable of forming biofilms.
In this work, the first biochemical characterization of PgaB is presented. We confirmed the activity of PgaB on β-1,6-GlcNAc oligosaccharides. The activity of PgaB is specific for the β-1,6 linkage and no de-N-acetylation of β-1,4-GlcNAc oligosaccharides was detected. Enzyme activity is dependent on the degree of substrate polymerization, as the second order rate constant for pentasaccharide substrate was determined to be four times higher than that of the tetrasaccharide substrate. Oligosaccharide sequencing studies indicate that there may be a pattern in the de-N-acetylation of substrates by PgaB. The central residue is modified in mono-de-N-acetylated pentasaccharide substrate, while di-de-N-acetylated hexasaccharide substrate shows modification mainly at the third and fifth residues from the non-reducing terminus of the substrate. Activity studies revealed that PgaB is activated by Ni2+ as well as by Fe2+, which is uncommon for deacetylase enzymes. Metal coordination to active site residues His184 and His189 was confirmed by mutagenesis studies, which also indicated that the metal likely plays a catalytic role. The results of these metal dependence studies support the observed binding of nickel and iron to the active site in PgaB crystal structures. The characterization studies presented in this thesis allow us to gain a better understanding of the de-N-acetylation aspect of the PNAG biosynthetic process and will serve as a basis for enzyme inhibitor design.Ph
Biochemical Characterization of Escherichia coli PgaB, an Enzyme Essential for Biofilm Formation
No full textThe formation of bacterial biofilms requires an extracellular matrix to facilitate adherence of bacteria to the surface they colonize. Carbohydrate polymers, known as exopolysaccharides, form a key component of most biofilm matrices. A wide variety of medically-important biofilm forming bacterial strains, including S. epidermidis, S. aureus, E. coli, B. pertussis, and Y. pestis generate the same β-1,6-N-acetyl glucosamine (PNAG) homopolymer as a key biofilm matrix exopolysaccharide. In E. coli, as well as in the other bacterial strains, the PNAG undergoes partial enzymatic de-N-acetylation, which is essential for surface attachment and subsequent biofilm formation. In vivo studies implied that the enzyme responsible for carrying out de-N-acetylation in E. coli is PgaB, an enzyme with sequence homologues in many Gram negative species capable of forming biofilms.
In this work, the first biochemical characterization of PgaB is presented. We confirmed the activity of PgaB on β-1,6-GlcNAc oligosaccharides. The activity of PgaB is specific for the β-1,6 linkage and no de-N-acetylation of β-1,4-GlcNAc oligosaccharides was detected. Enzyme activity is dependent on the degree of substrate polymerization, as the second order rate constant for pentasaccharide substrate was determined to be four times higher than that of the tetrasaccharide substrate. Oligosaccharide sequencing studies indicate that there may be a pattern in the de-N-acetylation of substrates by PgaB. The central residue is modified in mono-de-N-acetylated pentasaccharide substrate, while di-de-N-acetylated hexasaccharide substrate shows modification mainly at the third and fifth residues from the non-reducing terminus of the substrate. Activity studies revealed that PgaB is activated by Ni2+ as well as by Fe2+, which is uncommon for deacetylase enzymes. Metal coordination to active site residues His184 and His189 was confirmed by mutagenesis studies, which also indicated that the metal likely plays a catalytic role. The results of these metal dependence studies support the observed binding of nickel and iron to the active site in PgaB crystal structures. The characterization studies presented in this thesis allow us to gain a better understanding of the de-N-acetylation aspect of the PNAG biosynthetic process and will serve as a basis for enzyme inhibitor design.Ph
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