29 research outputs found
Author Correction: COVIDiSTRESS diverse dataset on psychological and behavioural outcomes one year into the COVID-19 pandemic (Scientific Data, (2022), 9, 1, (331), 10.1038/s41597-022-01383-6)
No full textThe original version of this Article contained an error in the spelling of the author Krzysztof Hanusz, which was incorrectly given as Hanusz Krzysztof. This has now been corrected in both the PDF and HTML versions of the Article. © The Author(s) 2023
Broad spectrum antimicrobial compounds from the bacterium Exiguobacterium mexicanum MSSRFS9.
No full textClinical bacterial pathogens front a major challenge for the clinical researchers and physicians. In particular microbial pathogens like Escherichia coli, Shigella flexneri, Klebsiella pneumonia and Salmonella enterica are apparelled with systemic machineries to bring down the human immune system as well as proliferate dramatically in a short period which in turn cause a pronounced ailment to the human health. In vitro evaluation of four purified compounds isolated from rhizosphere bacterium Exiguobacterium mexicanum tested against clinical pathogens mentioned above by disc diffusion method showed the two compounds viz., 3,6,18-trione, 9,10-dihydro-12'-hydroxyl-2methyl-5-(phenyl methyl) (5'-alpha, 10-alpha)-dihydroergotamine (C3) and dipropyl - S-propyl ester (C4) exhibit antibacterial property against all the tested pathogens. Among the four clinical pathogens tested, compound C3 has shown higher zone of inhibition against S. enterica with 17±0mm, followed by S. flexneri with 16.5±0.7mm, E. coli with 15±0mm and K. pneumoniae with 14±0mm, respectively. The compound C4 has shown higher antimicrobial activity against S. enterica with 21.5±0.7mm zone of inhibition, followed by S. flexneri with 19.5±0.7mm, E. coli with 17±0mm and K. pneumoniae with 16±0mm, these two compounds were found to be safer when subjected to rat haematological and enzymatic analysis
Combining neural and semantic features in the analysis of being supportive in online feedback from customers
Get PDFOver the past ten years, there has been a notable increase in the number of individuals accessing the internet. Positive evaluations serve as social evidence, convincing future purchasers of the product's quality and advantages. They can impact purchase decisions by offering real-world user information. Good reviews increase a product's or brand's trust and reputation. Customers are more inclined to buy from a firm that has received excellent feedback since it demonstrates dependability and contentment. Reviews can be considered user-generated content since they emphasise different applications, features, or advantages associated with a product. This material has the potential to persuade indecisive shoppers. The Yelp website was utilised to scrape feedback data for all Asian restaurants in New York City, which was then trained and assessed using three different models like Navie Bayes, next one is Logistic Regression, and then finally with Support Vector Classifiers. The Logistic Regression classifier outperformed the others by having the lowest proportion of mistakes and the highest Area under the ROC Curve noted as AUC on the receiver operating characteristic curve ROC curve. Commercial insights were gathered by recognising the existence of highly significant phrases while contrasting how they performed to the universal probabilities when the machine learning system was given review data from my restaurant
Sleep loss and sleep disorders: shedding light on common but under-recognised individual and community problems
Get PDFDarren R Mansfield, David R Hillman, Nicholas A Antic, R Doug McEvoy and Shantha M W Rajaratna
AN EXAMINATION OF AUSTRALIAN GENERAL PRACTITIONERS’ KNOWLEDGE, ATTITUDES AND PRACTICES IN RELATION TO SLEEP DISORDERS
No full textBackground: Sleep disorders represent an under-recognised public health problem and are reported to be underdiagnosed in general practices.Aims: To examine general practitioners’ (GPs) attitude, knowledge and practice behaviour and identify barriers to detection,diagnosis and treatment of sleep disorders encountered in the Australian primary care setting.Method: Using mixed methods, quantitative data from the Dartmouth Sleep Knowledge Questionnaire (DSKQ) were analysedusing MS Excel 2007. Qualitative data were obtained from one focus group and eight interviews. Data were thematicallyanalysed.Results: 15 GPs participated; seven in a focus group and eight in interviews. Scores from DSKQ suggest gaps in GPs’knowledge. Qualitative analysis revealed that patients frequently presented with sleep disorders underpinned by mentalhealth disorders. GPs agreed that prescribing pharmacological interventions was undesirable and behavioural interventionswere preferred. Barriers included limited training for GPs, lack of resources, patient expectations and willingness to engagein lifestyle changes, and consultation time constraints.Discussion: Greater flexibility to investigate sleep related problems within the standard consultation and improved accessto educational activities could assist GPs. Patient factors, such as adherence to management strategies, are paramount tosuccessful management of sleep disorders; however, these obstacles to clinical practice may be difficult to overcome.Conclusion: Providing education for GPs about sleep disorders, greater flexibility within consultations may improve patientcare and patient engagement in management strategies may assist, yet a critical success factor in disease managementincludes patient engagement in management strategies
Исследование морфологических признаков и параметров роста психрофильных микроводорослей и цианобактерий
Get PDFRecently, a question of producing a complex of biologically active substances from microalgae has aroused widespread interest. It is known that microalgae are able to produce a significant amount of exopolysaccharides. The aim of this work was to study morphological features and growth parameters of psychrophilic microalgae and cyanobacteria for the subsequent production of exopolysaccharides. The morphology of microalgae was observed using a binocular microscope. Growth parameters were studied by spectrophotometry; parameters of the culture medium were determined using a pH-meter. Exponential dependency graphs that show the dynamics and expected growth rate of microalgae were built. A rate of growth and polysaccharide biosynthesis in microalgae was determined upon changing the light intensity from 50 to130 mmol/m2/s. The highest level of cell counts in the logarithmic growth phase was up to 0.8 for Scenedesmus obtusiusculus Chod IPPAS S-329. A level of cells also varied in the deceleration phase from 0.25 for Ankistrodesmus acicularis Korsch IPPAS А-218 to 1.8 for Scenedesmus obtusiusculus Chod IPPAS S-329. Microalgae showed a high level of biomass accumulation under alkalophylic conditions. Eukaryotic algae actively photosynthesized at a pH of more than 8.0 and a temperature of 30 °C.The maximum activity at the level of pH 3.0/3.2 in the lag phase was 100% in C-1509 Nannochloris sp. Naumann. Microalga C-1509 Nannochloris sp. Naumann showed a high level of biomass accumulation under alkalophylic conditions; it photosynthesized at a pH of more than 8.0 and a temperature of 30 °C. It has been proved that neutrophiles can grow at pH lower than 3.0; this corresponds to the results of the experiments with the collection strains of microalgae with biomass productivity of 27.3%. At the alkaline pH values of 8.3-9.0, biomass productivity reduced from 46.0 to 37.2%. It is especially interesting that at the alkaline pH values of 7.5 and 8.0 biomass productivity of microalgae increased, which indicates the optimal growth conditions at this narrow pH range. An ability of microalgae to produce exopolysaccharides opens prospects of their use for practical purposes.В настоящее время широкий интерес приобрел вопрос получения комплекса биологически активных веществ из микроводорослей. Известно, что микроводоросли способны производить значительное количество экзополисахаридов. Целью данного исследования являлось изучение морфологических признаков и параметров роста психрофильных микроводорослей и цианобактерий для последующего получения экзополисахаридов. Морфологию микроводорослей рассматривали с помощью бинокулярной микроскопии. Параметры роста изучали с помощью спектрофотометрии, параметры культуральной среды выявляли с помощью рН-метрии. Были построены экспоненциальные графические зависимости, показывающие динамику и ожидаемую скорость роста микроводорослей. Определяли скорость роста и биосинтеза полисахаридов микроводорослей при изменении освещенности от 50 до 130 ммоль/м2/сек. Наибольший уровень количества клеток в фазе жизненного цикла — логарифмического роста составил до 0,8 для Scenedesmus obtusiusculus Chod IPPAS S-329. Уровень клеток варьировался также в фазе жизненного цикла — замедленного роста от 0,25 для Ankistrodesmus acicularis Korsch IPPAS А-218 до 1,8 для Scenedesmus obtusiusculus Chod IPPAS S-329. Микроводоросли показали высокий уровень накопления биомассы в алкалофильных условиях. Эукариотические водоросли активно фотосинтезировали с рН более 8,0 и температурой 30 °C. Максимальная активность на уровне лаг-фазы роста рН 3,0/3,2 для C-1509 Nannochloris sp. Naumann составляет 100%. Микроводоросль C-1509 Nannochloris sp. Naumann показала высокий уровень накопления биомассы в алкалофильных условиях, они фотосинтезировали при рН более 8,0 и температуре 30 °C. Доказано, что нейтрофилы могут расти при pH ниже 3,0, это соответствует результатам экспериментов с коллекционными штаммами микроводорослей, с продуктивностью по биомассе 27,3%. При щелочном рН 8,3-9,0 продуктивность по биомассе снижалась с 46,0 до 37,2%. Особый интерес представляет то, что при щелочных значениях рН 7,5 и 8,0 продуктивность микроводорослей по биомассе увеличилась, что указывает на оптимальные условия роста в этом узком диапазоне pH. Способность микроводорослей продуцировать экзополисахариды открывает перспективы их использования в практических целях
COVIDiSTRESS diverse dataset on psychological and behavioural outcomes one year into the COVID-19 pandemic
Get PDFDuring the onset of the COVID-19 pandemic, the COVIDiSTRESS Consortium launched an open-access global survey to understand and improve individuals’ experiences related to the crisis. A year later, we extended this line of research by launching a new survey to address the dynamic landscape of the pandemic. This survey was released with the goal of addressing diversity, equity, and inclusion by working with over 150 researchers across the globe who collected data in 48 languages and dialects across 137 countries. The resulting cleaned dataset described here includes 15,740 of over 20,000 responses. The dataset allows cross-cultural study of psychological wellbeing and behaviours a year into the pandemic. It includes measures of stress, resilience, vaccine attitudes, trust in government and scientists, compliance, and information acquisition and misperceptions regarding COVID-19. Open-access raw and cleaned datasets with computed scores are available. Just as our initial COVIDiSTRESS dataset has facilitated government policy decisions regarding health crises, this dataset can be used by researchers and policy makers to inform research, decisions, and policy. © 2022, The Author(s).U.S. Department of Education, ED: P031S190304; Texas A and M International University, TAMIU; National Research University Higher School of Economics, ВШЭThe COVIDiSTRESS Consortium would like to acknowledge the contributions of friends and collaborators in translating and sharing the COVIDiSTRESS survey, as well as the study participants. Data analysis was supported by Texas A&M International University (TAMIU) Research Grant, TAMIU Act on Ideas, and the TAMIU Advancing Research and Curriculum Initiative (TAMIU ARC) awarded by the US Department of Education Developing Hispanic-Serving Institutions Program (Award # P031S190304). Data collection by Dmitrii Dubrov was supported within the framework of the Basic Research Program at HSE University, RF
Implementing a Sleep Health Education and Sleep Disorders Screening Program in Fire Departments: A Comparison of Methodology
Get PDFObjective: The objective of this study is to compare three methods of administering a sleep health program (SHP) in fire departments. Methods: An SHP, comprising sleep health education and screening for common sleep disorders, was implemented in eight fire departments using three approaches: expert-led, train-the-trainer, and online. Participation rates, knowledge assessments, surveys, and focus group interviews were analyzed to assess the reach and effectiveness of the methodologies. Results: The Expert-led SHP had the highest participation rate, greatest improvement in knowledge scores, and prompted more firefighters to seek clinical sleep disorder evaluations (41%) than the other approaches (20 to 25%). Forty-two percent of focus group participants reported changing their sleep behaviors. Conclusion: All approaches yielded reasonable participation rates, but expert-led programs had the greatest reach and effectiveness in educating and screening firefighters for sleep disorders.Version of Recor
A co-registration method to validate in vivo optical coherence tomography in the breast surgical cavity
No full textBreast-conserving surgery accompanied by adjuvant radiotherapy is the standard of care for patients with early-stage breast cancer. However, re-excision is reported in 20-30 % of cases, largely because of close or involved tumor margins in the specimen. Several intraoperative tumor margin assessment techniques have been proposed to overcome this issue, however, none have been widely adopted. Furthermore, tumor margin assessment of the excised specimen provides only an indirect indication of residual cancer in the patient following excision of the primary tumor. Handheld optical coherence tomography (OCT) probes and their functional extensions have the potential to detect residual cancer in vivo in the surgical cavity. Until now, validation of in vivo OCT has been achieved through correlation with ex vivo histology performed on the specimen removed during surgery that is adjacent to the tissue scanned in vivo. However, this indirect approach cannot accurately validate in vivo imaging performance. To address this, we present a method for robust co-registration of in vivo OCT scans with histology performed, not on the main specimen, but on cavity shavings corresponding directly to the tissue scanned in vivo. In this approach, we use ex vivo OCT scans as an intermediary, surgical sutures as fiducial markers, and extend the in vivo field-of-view to 15 × 15 mm2 by acquiring partially overlapping scans. We achieved successful co-registration of 78 % of 139 in vivo OCT scans from 16 patients. We present a detailed analysis of three cases, including a case where a functional extension of OCT, quantitative micro-elastography, was performed
