3,333 research outputs found
Repliek I
No full textThe author\u27s reply to a critique of his book, Het Koninklijk Instituut voor de Marine (The Royal Naval Institute, no publisher, 1963), a study which combined participant observation (Lammers was trained at the Institute as a naval reserve officer) and a survey. Kruijer\u27s main objection was that Lammers overestimated the results of his survey at the expense of his participant observation. It is objected that: (1) the result of the comparison depends on the definition of the concept of \u27hyp,\u27 and (2) the general \u27equality\u27 of participant observation (ie with surveys) is not at issue in this case. I. Langnas
The Mr 30,000-33,000 major protein components of the lateral elements of synaptonemal complexes of the rat
No full textSynaptonemal complexes (SCs) are intranuclear structures which are formed during meiotic prophase between homologous chromosomes. The SC consists of two protein-rich axes, either of which is found at the basis of one of the homologous chromosomes. These axes, called lateral elements (LEs), are connected along their entire length by so-called transverse filaments. Between and parallel to the LEs runs a third element, called central element. The assembly and disassembly of SCs take place in a period during which a number of important events takes place at the chromosomal level: condensation, pairing, recombination and segregation of homologous chromosomes. The possible involvement of the SC in these events is an importnat topic in the research program of our section. This thesis focusses on the possible function of the LE and its components (Chapter 1). The experimental work described in this thesis (Chapters 2-5) concerns the characterization of the LE-components with relative electrophoretic mobilities of 30,000 and 33,000 (the M r 30,000-33,000 components).The isolation of cDNAs encoding the M r 30,000-33,000 components is described in Chapter 2. The isolation took place by screening of an expression cDNA-library with an affinity-purified polyclonal antiserum. The largest cDNA, 2A4, encodes a protein with a predicted molecular mass of 29.7 kDa, which we have termed s ynaptonemal c omplex p rotein 3 (SCP3). A polyclonal antiserum raised against SCP3 recognizes only the M r 30,000-33,000 components on a westernblot of SC-protein and exhibits a similar immunological localisation as monoclonal antibodies and a polyclonal antiserum raised against the M r 30,000-33,000 components. The deduced amino acid sequence shows that SCP3 is a potential ATP-binding protein and that the C-terminal half of the protein is capable of forming an amphipaticα-helix. Moreover, part of the amino acid sequence exhibits considerable homology to the predicted protein products of two members of a gene familiy of X-linked lymphocyte-regulated ( Xlr ) genes.We conclude that cDNA 2A4 encodes at least one of the M r 30,000-33,000 components and that SCP3 is a major component of the LEs of the rat. We speculate that the homology between SCP3 and two products of Xlr genes might be connected to a function of both types of protein in processes which share the common purpose of blocking certain recombination events.The M r 30,000- and the 33,000 component are closely related according to their almost identical peptide maps and the fact that all isolated antibodies always recognize both proteins. A first step to elucidate the difference between these two components and the level at which such a difference arises is described in the addendum to Chapter 2. We conclude that the M r 30,000-33,000 components are probably encoded by a single gene and a single messenger RNA and that the difference between the two components arises during or after translation. Mechanisms by which this could be achieved are discussed. A practical problem which arises here is that N-terminal sequencing of the two components by means of standard techniques is not possible.When SC-protein is separated by means of two-dimensional gel electrophoresis (2D-electrophoresis) and the gel is subsequently blotted and the blot used for immunological detection of the M r 30,000-33,000 components, a large number of variants, differing in isoelectric point and relative electrophoretic mobility can be discerned. In Chapter 3 experiments are described which were performed to determine (i) the nature of the differences between the variants and (ii) possible changes in the observed pattern on 2D-gels during subsequent stages of meiotic prophase. We conclude that differences in the number of attached phosphate groups are largely responsible for the existence of different variants of the M r 30,000 as well as the M r 33,000 component. Moreover, we observed a change in the phosphorylation pattern between early- and midpachytene, probably because of the addition of one phosphate group to all variants. We speculate that the phosphorylation pattern observed as early as zygotene is the result of the action of a kinase which responds to DNA-damage.The presence of the LEs at the bases of the chromatin of the homologous chromosomes prompted us to investigate whether points of contact exist between LEs and the chromatin, and if so, which LE-components and DNA-sequences are involved (Chapter 4). Based on crosslinking experiments in vivo of chromatin from mouse spermatocytes, we assumed that the M r 30,000-33,000 components are bound to DNA or at least associated with DNA. By immunoprecipitation of protein-DNA complexes, obtained after crosslinking in vivo of mouse spermatocytes, with antibodies raised against the M r 30,000-33,000 components as well as against isolated SCs, a region was identified within a 120 kbp gene cluster as a SC - a ssociated r egion (SCAR). This SCAR colocalizes with a so-called 'matrix attachment region' (MAR). We also developed a method by which specific binding of DNA-fragments to the SC can be tested in vitro . By means of this method we show that MARs from different organisms bind specifically to rat SCs. We conclude that the organisation of the chromatin during meiotic prophase shows a certain similarity to those during mitosis and interphase.After it was determined that the M r 30,000-33,000 components are probably bound to DNA in vivo , we have further analysed the possible DNA-binding of these proteins as well as SCP3 in vitro (Chapter 5). Because of the insolubility of SCP3 in aqueous solutions, these studies were carried out by means of the Southwestern blotting technique. We conclude that SCP3 binds to DNA in vitro and shows a preference for single-stranded DNA. From a comparison of different single-stranded DNA substrates we conclude that SCP3 probably favours binding to single-stranded DNA which adopts a spatial conformation caused by intra- or intermolecular interactions. In similar experiments the M r 30,000-33,000 components also exhibit a preference for single-stranded DNA, although the variants which caary the most phosphate residues show no detectable DNA-binding at all. Based on the assumption that in the situation in vivo the M r 30,000-33,000 components would exhibit a similar type of DNA-binding as in vitro , we speculate on the (temporal) existence of single-stranded DNA during meiotic prophase.Finally, I present a summary on what is known about the M r 30,000-33,000 components at the beginning of the general discussion (Chapter 6). Then I discuss a model which describes the chromatin organization during meiotic prophase, in which I particularly point at the modifications which, starting from a general organization pattern, are necessary for the proper progress of the processes of pairing, recombination and segregation of homologous chromosomes. Within this model I discuss the possible involvement of components of the SC. After that I focuss on the function of the M r 30,000-33,000 components in this model. I propose that the M r 30,000-33,000 components play a role in the structural organization of the chromosomes, in such a manner that recombination between sister chromatids is inhibited temporarily and cohesion between sister chromatids is maintained as long as this is needed.</p
"Have you not read this Scripture?":Memory Variation and Context-Based Modification in the Old Testament Quotations in the Gospel of Mark
No full textIn the study presented in this thesis, Hans Lammers sets out to answer the question which Old Testament (OT) version was used by the Markan Evangelist as the source of his scriptural quotations.Next, he observes that in most cases, the text of the quotations in Mark deviates from that in the OT source used. Whereas scholars usually ascribe the observed variation to the use of variant versions of the OT text that are no longer extant, Lammers offers a different explanation. He argues that the Markan author quoted the OT from memory, a process in the course of which a considerable portion of the observed variation was generated. This explanation requires us to modify current views of evangelists sifting through biblical books from which they copy certain passages. Lammers suggests that another part of the observed variation may be the result of conscious adaptation of OT quotations making them better serve the purpose of supporting the Gospel’s message. By means of four case studies, he tries to demonstrate how views of Jesus developed in the Markan Gospel may have prompted the Evangelist to alter OT quotations, a practice for which he proposes the designation context-based modification.In het onderzoek dat beschreven wordt in dit proefschrift gaat Hans Lammers op zoek naar het antwoord op de vraag welke versie van het Oude Testament door de evangelist Marcus werd gebruikt als bron voor de Schriftcitaten in zijn evangelie. Vervolgens stelt hij vast dat de tekst van de citaten in Marcus in de meeste gevallen afwijkt van die van de geciteerde teksten in de gebruikte versie van het Oude Testament. Waar onderzoekers deze variatie gewoonlijk toeschrijven aan het gebruik van variante versies van de tekst van het Oude Testament die verloren zijn gegaan, stelt Lammers een andere verklaring voor: Marcus heeft het Oude Testament uit zijn hoofd geciteerd en daarbij is een aanzienlijk deel van de variatie ontstaan. Deze verklaring vereist een aanpassing van het traditionele beeld waarbij evangelisten worden afgebeeld te midden van bijbelboeken waaruit zij passages overschrijven. Een ander deel van de waargenomen variatie zou volgens Lammers het gevolg kunnen zijn van bewuste aanpassing van Oude Testament citaten zodat ze de boodschap die het evangelie wil overbrengen beter ondersteunen. In een viertal case studies probeert hij aan te tonen hoe visies op Jezus die in het Marcusevangelie worden ontwikkeld aanleiding kunnen zijn geweest voor Marcus om Oude Testament citaten aan te passen, een gebruik waarvoor hij de benaming context-based modification voorstelt.<br/
High Aspect Ratio Bioprinted Structures
No full textThese files constitute the dataset for evaluating 3D Bioprinting of high aspect ratio structures. Material rheology data, 3D printing geometric data, and the python code used for generating the plots are shared here
Lichtspur
No full textA 5-hour durational performance collaboration with artist Christoph Lammers and local musicians in St. Pauls, Munich as part of the festival Die Lange Nacht der Musik, April 201
Lichtung
No full textAn exhibition project by artist Christoph Lammers with guest artists Stefanie Sachsenmaier and Mie Imori, Unicredit Kunstraum, Munich, December 201
Tanz auf dem Vulkan (Dancing on a volcano)
No full textSolo Performance
Collaboration with artist Christoph Lammers
Sound Simjon Spengler
Drawing, movement and sound exist in the same space. A three-hour live performance installation took place at the private view on 27 April. A film of the event was screened next to the remaining charcoal wall drawing
Does influence beget autonomy? Clarifying the relationship between social and personal power
No full textWe iteratively develop and test a model to clarify the relationship between both high and low levels of social (influence) and personal (autonomy) power. A meta-analysis synthesising primary data (n = 298) and secondary data (n = 498) found that impaired personal power coincided with impaired social power, but not vice versa. Unexpectedly, elevated social power did not coincide with elevated personal power, suggesting that the association between influence and autonomy attenuates with increasing levels of power. Predictions arising from the meta-analysis and our revised theoretical model were supported in a subsequent study (n = 266). We discuss implications of these findings and avenues for future research
Fabrieksontwerp: De bereiding van vinyl-tolueen
No full textDocument(en) uit de collectie Chemische ProcestechnologieDelftChemTechApplied Science
Nordwestdeutschland und seine Regionen im Standortwettbewerb
No full textNordwestdeutschland hat sich seit Ende der 80er Jahre günstiger entwickelt als das übrige westdeutsche Bundesgebiet. Damit hat sich ein langanhaltender räumlicher Entwicklungstrend umgekehrt. Die Ursachen hierfür liegen teils in der spezifischen Wirtschaftsstruktur Nordwestdeutschlands begründet, teils sind sie aber auch auf eine verbesserte Standortsituation zurückzuführen. So hat die deutsche Einheit und die Öffnung Osteuropas die Region besonders begünstigt. Nordwestdeutschland konnte seine Attraktivität für das Sachkapital steigern, allerdings nicht für das Humankapital. Unter anderem deshalb ist nicht sichergestellt, daß Nordwestdeutschland dauerhaft auf einen steileren Wachstumspfad eingeschwenkt ist. Hamburg ist seit Ende der 80er Jahre zur treibenden Kraft der wirtschaftlichen Entwicklung in Nordwestdeutschland geworden. Die Stadt nimmt zunehmend eine Metropolfunktion für die Region wahr. Die administrative Gliederung in Stadtstaaten und Flächenländer stellt einen Nachteil für Nordwestdeutschland im Standortwettbewerb dar, da vielfach miteinander verflochtene Wirtschaftsräume administrativ zerschnitten werden. Dadurch sind die Bedingungen für eine optimale Standortpolitik in dieser Region weniger günstig als anderswo. Die folgenden Ausführungen stellen das zusammenfassende Kapitel einer Untersuchung dar, die im Rahmen der Arbeitsgruppe „Strukturberichterstattung Norddeutschland” der Landesarbeitsgemeinschaft Bremen/Hamburg/Niedersachsen/Schleswig-Holstein der Akademie für Raumforschung und Landesplanung durchgeführt wurde. Vgl. Akademie für Raumforschung und Landesplanung (Hrsg.): Landesentwicklung in Norddeutschland, Nordwestdeutschland und seine Regionen im Standortwettbewerb. — Hannover 1997. Diese Veröffentlichung beinhaltet Einzeluntersuchungen von: Lammers, K.: Die sektorale Spezialisierung Nordwestdeutschlands und seiner Regionen, S. 51–81; Legler, H.; Machate-Weiß, V.: Zukunftsorientierung der nordwestdeutschen Wirtschaft — Investitionen in Sachanlagen, Forschung & Entwicklung und Humankapital, S. 83–118; Richert, R.: Nordwestdeutschlands Verflechtungen mit dem Ausland, S. 119–157; Voss, G.: Die Entwicklung der Wirtschaftsleistung des Einkommens und der Beschäftigung in Nordwestdeutschland, S. 19–49. Auf deren Ergebnissen basiert das zusammenfassende Kapitel
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