107 research outputs found
Investigating chlorite dismutase biocatalyst reuse using columns
Get PDFMicrocontaminants are compounds in water present below 1 ppm which have major long-term health impacts. Traditional treatment processes are not designed to treat these chemicals and existing treatment methods come with downsides including higher chemical input, higher energy input, production of a waste stream, use of high cost media, and/or production of toxic byproducts. Biocatalysis, using enzymes to degrade contaminants, has been proposed as a treatment method. This process would require reuse of the biocatalysts to reduce costs for continuously operated water treatment systems. To study the potential for biocatalyst reuse, experiments were conducted on a chlorite dismutase biocatalyst modified with a strep tag; this reuse system allows the biocatalyst to bind to coated resin beads that can be reused in a column system. Chlorite dismutase treats chlorite to chloride and oxygen. Activity tests were conducted in batch for the purified and attached biocatalysts and in column to determine impact of attachment on the biocatalyst’s activity and inactivation. Activity results showed no impact of tag addition, with a tagged biocatalyst Vmax of 34,600 ± 2,900 μmol chlorite min-1 μmol heme-1 and a Km of 106.6 ± 70.6 μM. The attached biocatalyst was able to reach the same maximal activity as the free biocatalyst with the introduction of a mass transfer limited region, which can be described with a kl of 0.1415 cm min-1. Column activity was difficult to measure but was similar to the attached batch result in four successful experiments. Finally, the maximum theoretical turnover number in the column system was 152,800 ± 11,800 μmol chlorite μmol heme-1. While more understanding of the inactivation kinetics is needed to enable a detailed comparison, based on the maximum theoretical turnover number, a fully loaded column is projected to be able to treat 86,000 bed volumes (equivalent to 59.7 days at design flowrate and concentration) before being fully inactivated.Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2021-12-01The student, Indran Kamalanathan, accepted the attached license on 2019-10-08 at 21:50.The student, Indran Kamalanathan, submitted this Thesis for approval on 2019-10-08 at 22:04.This Thesis was approved for publication on 2019-10-09 at 15:49.DSpace SAF Submission Ingestion Package generated from Vireo submission #14475 on 2020-02-28 at 17:35:37Made available in DSpace on 2020-03-02T22:38:38Z (GMT). No. of bitstreams: 2
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Previous issue date: 2019-10-09Embargo set by: Seth Robbins for item 113974
Lift date: 2022-03-02T22:39:04Z
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Turner′s syndrome presenting as metabolic bone disease
No full textTurner′s syndrome is a genetic disorder with a complete or partial absence of one X chromosome with characteristic phenotypic features. The prevalence of renal anomalies in turner syndrome is 30-40%. However, the renal function is usually normal. We report a case of Turner′s syndrome presenting with chronic kidney disease and renal osteodystrophy
Mixing and regime transition analysis of liquid-solid conical fluidized bed through RPT technique
No full textThe current work focuses on the hydrodynamic study of liquid-solid mono and binary conical fluidized bed using the radioactive particle tracking technique. Two different sizes 0.6 and 1 mm diameter glass beads were used for the experiment. Two different bed compositions, mono dispersed (100 wt% of each solid) and 50 wt% mixture of the solids, were studied. A rich data base, including instantaneous velocities, mean and RMS velocities, granular temperature, etc., were calculated by suitable post-processing. Further, Hurst exponent, autocorrelation function, segregation index, Kolmogorov entropy, and correlation dimension were calculated using time series data to understand the mixing and chaotic nature of the fluidized bed for each set of data. Good degree of mixing was observed even at a low velocity of 0.07 m/s. Kolmogorov entropy and correlation dimension indicate regime change at liquid inlet velocity of 0.1 m/s for both mono and 50 wt% binary bed.Fil: Kalo, Lipika. Indian Institute of Technology Guwahati; IndiaFil: Kamalanathan, Premkumar. Bhabha Atomic Research Centre; IndiaFil: Pant, Harish J.. Bhabha Atomic Research Centre; IndiaFil: Cassanello Fernandez, Miryam Celeste. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias. Instituto de Tecnología de Alimentos y Procesos Químicos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Tecnología de Alimentos y Procesos Químicos; ArgentinaFil: Upadhyay, Rajesh K.. Indian Institute of Technology Guwahati; Indi
Investigating chlorite dismutase biocatalyst reuse using columns
No full textMicrocontaminants are compounds in water present below 1 ppm which have major long-term health impacts. Traditional treatment processes are not designed to treat these chemicals and existing treatment methods come with downsides including higher chemical input, higher energy input, production of a waste stream, use of high cost media, and/or production of toxic byproducts. Biocatalysis, using enzymes to degrade contaminants, has been proposed as a treatment method. This process would require reuse of the biocatalysts to reduce costs for continuously operated water treatment systems. To study the potential for biocatalyst reuse, experiments were conducted on a chlorite dismutase biocatalyst modified with a strep tag; this reuse system allows the biocatalyst to bind to coated resin beads that can be reused in a column system. Chlorite dismutase treats chlorite to chloride and oxygen. Activity tests were conducted in batch for the purified and attached biocatalysts and in column to determine impact of attachment on the biocatalyst’s activity and inactivation. Activity results showed no impact of tag addition, with a tagged biocatalyst Vmax of 34,600 ± 2,900 μmol chlorite min-1 μmol heme-1 and a Km of 106.6 ± 70.6 μM. The attached biocatalyst was able to reach the same maximal activity as the free biocatalyst with the introduction of a mass transfer limited region, which can be described with a kl of 0.1415 cm min-1. Column activity was difficult to measure but was similar to the attached batch result in four successful experiments. Finally, the maximum theoretical turnover number in the column system was 152,800 ± 11,800 μmol chlorite μmol heme-1. While more understanding of the inactivation kinetics is needed to enable a detailed comparison, based on the maximum theoretical turnover number, a fully loaded column is projected to be able to treat 86,000 bed volumes (equivalent to 59.7 days at design flowrate and concentration) before being fully inactivated.LimitedAuthor requested closed access (OA after 2yrs) in Vireo ETD syste
Synopeas pauropsyllae Veenakumari & Buhl & Mohanraj 2018, sp. nov.
No full textSynopeas pauropsyllae Veenakumari & Buhl sp. nov. (Figs 1–8) Material examined. HOLOTYPE: ♀, INDIA: KARNATAKA : Bengaluru, Hebbal, 13°02 ′ 08 ″ N 77°35 ′ 49 ″ E, 906 m, 02.XII.2011 (NBAIR, registration no. ICAR / NBAIR / P1920). PARATYPES: 11 ♀♀ (nos. ICAR / NBAIR / P1921–P1931) and 2 (nos. ICAR / NBAIR /P1932–P1933), same data as holotype (NBAIR). Description. Adult female . Body length: 1.24–1.37 mm (mean = 1.3, SD = 0.04, n = 11; holotype = 1.3 mm). Colour. General body colour black. A1 and legs golden yellow except dark brown coxae, apices of femur and tibia and basitarsi of hind leg; clava dark brown, other antennomeres golden yellow (Figs 1, 5). Head (Figs 1, 2, 4, 5). FCI = 1.23; LCI = 1.44. IOS 0.65× width of head, shortest at the level of mid-frons; frons finely reticulate, sparsely setose; inverted V shaped striae present above toruli; hyperoccipital carina distinct, joining the posterior orbits; hyperoccipital carina extending anteromedially; occiput finely reticulate; compound eye bare, L/W= 19.4/13.1; posterior ocellus contiguous with orbits; POL>LOL in ratio of 24.4/10.5; clypeus rectangular (L/W = 2.3/12.7); interantennal process projecting; length to width of antennomeres in ratios of 25.1/5.1, 6.7/3.1, 2.8/1.9, 5.5/2.0, 2.5/2.3, 3.7/3.0, 4.2/4.7, 5.1/5.7, 5.1/6.6, 7.7/5.7, respectively; radicle 0.1× length of A1. Mesosoma (Figs 1–3, 6, 8). Mesoscutum as long as wide (L/W = 29.5/30.6), convex, sparsely setose, finely reticulate, reticulations longitudinally elongate medially; posteromedial margin of mesoscutum extending as a short triangular midlobe over mesoscutellum; axilla large, triangular, covered with 12 long white setae; scutoscutellar sulcus non-foveate; pronotum clearly visible from above, reticulate, sparsely setose; epomial carina faint; cervical pronotal area finely reticulate with anterior rim of pronotum smooth; upper lateral pronotal area reticulate and sparsely setose; lower lateral pronotal area smooth; entire mesopleuron smooth; metapleuron smooth with dense long white setae; mesoscutellum triangular, convex, smooth with a short upturned spine with a lamella posteromedially, L/W = 13.7/20.4; lateral keel broad anteriorly and narrow posteriorly; metascutellum rectangular, smooth; metanotal trough smooth with two transverse median carinae (Fig. 8); propodeum with two median carinae placed close to each other; lateral propodeal area smooth with dense long white setae. Forewing (L/W = 88.7/39.4) and hind wing (L/W = 77.5/14.1) hyaline, with sparse microtrichia; forewing with no marginal cilia; hind wing cilia 0.25× width of wing. Metasoma (Figs 1, 6). L/W = 41.6/34.4; T1 short with median and submedian carinae; T2, entirely smooth with a row of long white setae on anterior margin; remaining tergites anteriorly smooth and with faint reticulations and sparse setae posteriorly; length and width of tergites T1–T 6 in ratios of 3.9/11.6, 30.3/18.2, 2.3/32.7, 2.0/28.1, 1.4/24.1, 3.5/18.7, respectively; anterior margin of S2 with dense setae. Adult male. Similar to female. Body length 1.24–1.28 mm (mean = 1.26, SD = 0.03, n = 2). Length and width of antennomeres A1–A 10 in ratios of 25.6/5.2, 6.2/3.2, 2.2/2.1, 5.8/2.6, 4.3/2.5, 5.0/3.1, 6.0/3.6, 6.1/3.7, 6.0/3.7, 10.0/3.5, respectively (Fig. 7). A3–A10 densely setose, length of setae 0.1× length of A1. Metasoma L/W = 37.3/30.1, with seven tergites; length and width of tergites T1–T 7 in ratios of 3.6/14.1, 24.0/17.6, 2.9/29.3, 2.9/26.5, 2.1/21.2, 1.7/14.8, 1.3/8.3, respectively. Variability. Not much variation was found between specimens except for differences in size as mentioned above. This could possibly be the result of all specimens being obtained from a single batch of galls. Differential diagnosis. This new species of Synopeas is similar to S. involutum Kieffer, 1926, S. pinnei Buhl, 2009, S. mangiferae Austin, 1984 and S. fuscum Buhl, 1998 but differs from them in the following combination of character states: presence of hyperoccipital carina, OOL not distinct, dense setae along hind margin of mesoscutum, shape of scutellum and unusually short female T3–T6. While S. involutum, S. pinnei and S. fuscum are distributed in the Palaearctic Region, S. mangiferae is an Oriental species known from India (KIEFFER 1926; AUSTIN 1984; BUHL 1998, 2009). Biology. All specimens emerged from galls induced by Pauropsylla cf. depressa (Hemiptera: Psylloidea: Triozidae) on isolated trees of Ficus benghalensis (Figs 9–12) situated in rainfed agricultural land. The adult male psyllid that emerged from the galls closely resembles P. depressa Crawford, 1912 as redescribed by MATHUR (1975) in the structure of the head and antenna, the shape and venation of the fore wing and probably also in the paramere shape, but a more detailed study of the material is needed to confirm the identity (I. Malenovský & D. Burckhardt, pers. comm.). Pauropsylla depressa is a widely distributed species in India including Karnataka; it has so far been known to induce galls on Ficus racemosa L. (MATHUR 1975, D’SOUZA & RAVISHANKAR 2014). Etymology. This species is named ‘ pauropsyllae ’ based on the generic name of the host of this parasitoid; noun in genitive case standing in apposition.Published as part of Veenakumari, Kamalanathan, Buhl, Peter Neerup & Mohanraj, Prashanth, 2018, A new species of Synopeas (Hymenoptera: Platygastridae) parasitizing Pauropsylla cf. depressa (Psylloidea: Triozidae) in India, pp. 137-141 in Acta Entomologica Musei Nationalis Pragae 58 (1) on pages 138-140, DOI: 10.2478/aemnp-2018-0011, http://zenodo.org/record/367670
Self-rectifying non-volatile tunneling synapse: multiscale modeling augmented development
No full textAn atypical, brachypterous species of Idris Förster (Platygastroidea: Scelionidae) from India
Get PDFA new species of Idris – Idris semiflaviventris sp. n. - belonging to the tribe Baeini is described from Kodaikanal in the Western Ghats, S. India. This species is unusual in that it has a short interocellar space, subequal posterior margins of T1 and T2, and T2 the longest and widest tergite
Inostemma indicum Mani 1941
No full textInostemma indicum Mani (Figs 1–11) Female: Body length = 2.116 mm; (m=2.110 (2.028-2.148) mm, SD=0.03, n=15) Colour: Entire body black; legs honey brown except for black coxa, hind femur and distal tarsomere; radicle honey brown; A1 honey brown except for yellow patches laterally and apically; remaining antennomeres black except A2, A5, A6 which have a brown tinge (Figs 1, 2). Head (Figs 1, 2, 3, 5, 9): FCI= 1.42; LCI= 1.32; IOS 0.144× eye length; compound eye large (L: W= 21.6:19.1) with sparse minute setae; POL>LOL>OOL in ratio of 17.3:8.4:2.7; posterior ocelli away from orbits, OOL 0.46× OD; frons, vertex, gena finely reticulate, sparsely setose; hyperoccipital carina absent, occiput finely reticulate, uniformly setose; length and width of antennomeres A1–A 10 in ratio of 26.0:5.5, 7.9:3.4, 6.5:3.3, 4.8:3.7, 2.4:2.7, 3.7:3.5, 4.1:5.2, 5.4:5.8, 5.0:5.8, 7.7:4.5, respectively; A1 lamellate apically; radicle 0.13× length of A1. Mesosoma (Figs 3, 10, 11): Mesoscutum (L: W=34.7:40.6) finely reticulate; notauli complete, posteriorly converging; a short midlobe posteromedially extending onto mesoscutellum; antero-admedian lines indicated; parapsidal ridges present; lateral pronotal area finely reticulate, uniformly setose, with a smooth depression anteromedially; anterior rim of pronotum reticulate-striate; pronotal groove smooth; mesopleuron anteriorly weakly reticulate, remainder smooth, with weak striae dorsally and reticulations ventrally; transepisternal line wide and triangular; mesopleural depression smooth; metapleuron covered with dense setae; scutoscutellar sulcus with large foveae on posterior margin. When horn removed: scutellar disc (L: W:19.5:21.0) finely reticulate with foveae on anterolateral margin; posterior margin of scutellar disc with a inverted ‘U’ shaped carina; posterior and posterolateral margin of scutellar disc predominantly smooth; metascutellum distinct, striate with weak foveae in between; metanotal trough predominantly smooth; lateral propodeal area unevenly sculptured, sparsely setose. Fore wing (L: W= 101.7:45.4) with a long submarginalis, 0.38× length of wing, ending with an elongate knob; microtrichia on fore wing uniformly setose in distal half and absent in basal half; hind wing (L: W=89.8:21.8) with sparse microtrichia; fore wing marginal cilia and hind wing marginal cilia 0.05× and 0.21× width of their respective wings. Metasoma (Fig. 6): (L: W=98.9:37.7); T 1 in female with a long horn (L:W=73.5:10.5), 0.75× length of metasoma, extending up to anterior margin of mesoscutum; T1 medially striate, of which two medial striae extend on to base of horn; horn finely transversely reticulate; T1 densely setose laterally; T2 anteromedially rugose and sparsely setose, laterally striate, striae extending 0.45× length of tergite and a few sparse lateral striae almost reaching the posterior end of tergite, remainder smooth; T3–T5 anteriorly reticulate and posteriorly smooth and each tergite with two transverse rows of setae; T6 entirely reticulate, setose and subequal in length and width; length and width of tergites T1 (exclusive of horn) –T 6 in ratio of 12.5:19.9, 46.3:23.7, 9.0:35.9, 8.1:30.7, 6.9:23.1, 15.2:15.1, respectively. Male (Figs 7, 8): Body length = 1.885 mm; (m=1.899 (1.864–2.01) mm, SD=0.05, n=15)。 Male similar to female except for the absence of horn and antennal characters; length and width of antennomeres A1-A 10 in ratio of 24.0:5.0, 6.7:3.6, 6.7:3.7, 7.0:3.1, 4.5:3.3, 6.8:4.2, 6.2:4.2, 6.5:4.0, 6.4:3.8, 10.1:3.9, respectively. T1 anteromedially smooth followed by costae, densely setose laterally; T2 anteriorly with an oblique carina oriented towards middle, beneath which short longitudinal striae present; T2 entirely smooth except anteromedially. Material examined: Neotype female, (ICAR / NBAIR /P1935), INDIA: Karnataka: Chikkaballapur, 13°25'56''N 77°43'40''E, 920m, 12.IX.2017, Coll. A. N. Shylesha. 23 females, (ICAR / NBAIR /P1936–P1958) and 19 males (ICAR / NBAIR /P2495–P2513), with same data as neotype. Host: These parasitoids emerged from stem galls on Coccinia grandis caused by Neolasioptera cephalandrae (Figs 12–14). Diagnosis: Inostemma indicum females share the presence of a long horn on T1 with two other species from India, I. berijamum, and I. coorgense. This species differs from I. berijamum in the following character states: In I. berijamum fore wing densely setose, horn on T1 curved and away from body reaching anterior ocellus, T6 at least 1.8× as long as wide. Whereas in I. indicum microtrichia of fore wing sparse in basal half and horn on T1 not curved, almost adjacent to mesoscutum and not reaching head, length and width of T6 subequal. This species also differs from I. coorgense (type material unknown, Vlug, 1995) where vertex is smooth, gena sparsely punctate, horn on T1 extending ahead of posterior ocelli, mesoscutum smooth and shiny whereas in I. indicum vertex and gena finely reticulate, horn on T1 not reaching head, mesoscutum finely reticulate. Acknowledgment: The authors are thankful to the Director, NBAIR, Bengaluru for providing facilities for carrying out this work. We are grateful to Dr. D. Dey for examining the type collection at the National Pusa Collection and indicating that the holotype of I. indicum is not present there. We thank Dr. John S. Noyes, History Museum, London; Dr. E. J. Talamas, DPI, Florida Mr. Peter N. Buhl for taxonomic discussion. We also thank V. Shashikala for all help rendered. We thank Dr. K. J. David for identifying the gall midge. Literature support by “The Platygastroidea Planetary Biodiversity Inventory Project” is acknowledged.Published as part of Kamalanathan, Veenakumari, Shylesha, A. N. & Mohanraj, Prashanth, 2018, Neotype designation and redescription of Inostemma indicum (Platygastroidea: Platygastridae) parasitizing ivy gourd gall midge, pp. 439-444 in Zootaxa 4420 (3) on pages 440-444, DOI: 10.11646/zootaxa.4420.3.9, http://zenodo.org/record/125095
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