25 research outputs found
Analisis Pendapatan Dan Profitabilitas Usaha Pertenakan Ayam Petelur Aditya Farm Jorong Parumpung Nagari Koto Baru Simalanggang Kecamatan Payakumbuh Kabupaten Lima Puluh Kota
No full textPenelitian ini bertujuan untuk menganalisis pendapatan dan profitabilitas usaha
peternakan ayam ras petelur Aditya Farm. Penelitian dilaksanakan di peternakan ayam
ras petelur Aditya Farm Jorong Parumpung Kabupaten Lima Puluh Kota pada tanggal
12 Januari 2022 selama 1 bulan. Metode penelitian yang digunakan adalah metode
studi kasus. Data yang digunakan dalam penelitian ini adalah aspek teknis : bibit pullet,
kandang, pakan, tatalaksana pemeliharaan, pencegahan dan pengendalian penyakit,
pemasaran. Pada aspek ekonomi meliputi: biaya produksi pada periode Januari 2020
s/d Desember 2021 yang terdiri dari biaya tetap dan biaya variabel, penerimaan
bersumber dari hasil penjualan telur dan penjualan ayam afkir. Hasil penelitian
menunjukkan bahwa aspek teknis yang digunakan pada peternakan ayam ras petelur
Aditya Farm sudah cukup baik. Biaya produksi yang dikeluarkan pada periode Januari
2020 - Desember 2021 sebesar Rp. 24.489.877.523 dan penerimaan yang diterima
sebesar Rp. 25.880.177.307. Pendapatan yang didapatkan yang didapatkan oleh Aditya
Farm selama Januari 2020 - Desember 2021 adalah sebesar Rp.1.390.299.784 dengan
R/C Ratio sebesar 1,06 yang berarti usaha peternakan ayam ras petelur yang dijalankan
oleh Aditya Farm tergolong menguntungkan
Recommended from our members
Role of Mitochondria in HIV Infection
No full textHIV-1 hijacks our cellular machinery to complete its life cycle. A better understanding of the interactions between cellular proteins and viral components will certainly lead to the discovery of new ways to inhibit viral replication. My primary research goals are directed toward understanding the basis of molecular interactions between viral and host factors (proteins or organelles) during infection of human cells. In my thesis, the role of mitochondria in the early stage of HIV infection has been evaluated by using a VSV-G pseudotyped HIV-based lentiviral vector. I have observed that cells lacking mitochondrial DNA (ρ0 cells), isolated from an established human osteosarcoma cell line, are defective in the ability to support virus infection when compared to their parental cells. This infection deficiency in ρ0 cells can be repaired by reintroducing mitochondria from ρ+ human cells (293T cells) using transmitochondrial technology. Inhibition of oxidative phosphorylation by mitochondrial inhibitors did not inhibit HIV infection in HOS cells (ρ+ cells) indicating that the reduced infection efficiency in ρ0 cells is not simply the result of reduced oxidative phosphorylation. Further analysis indicates that virus infection in ρ0 cells is blocked at steps after reverse transcription and before nuclear import. Confocal fluorescence microscope analysis shows the subcellular location of the majority of virus nucleoprotein particles (identified by the presence of the viral capsid protein) to be near or in contact with mitochondria. Co-fractionation of viral complexes with mitochondria from infected cells also supports the hypothesis of association of viral complexes with mitochondria. In conclusion, in my study I have shown that ρ0 cells are defective in the ability to support HIV infection and viral infection is inhibited at steps after reverse transcription and before nuclear import. Mitochondria may play an important role in the intracellular transport by directly association with viral complexes. A role for mitochondrial association of viral intracellular complexes has not been previously explored. The elucidation of this role will open new avenues for investigation of the early steps in HIV infection.</p
Apolipoprotein E4 Suppresses Neuronal-Specific Gene Expression in Maturing Neuronal Progenitor Cells Exposed to HIV
No full textThe apolipoprotein ε4 gene allele and the apolipoprotein E4 protein (ApoE4) are important host susceptibility factors linked to neurocognitive disorders associated with HIV infection or Alzheimer's disease. Our previous studies showed differential effects of the two most common human ApoE genotypes, APOE3/3 and APOE3/4, on gene expression by differentiating human neuroepithelial progenitor cells continuously exposed to HIV. To investigate the effects of ApoE3 versus ApoE4 isoforms specifically on maturing neurons, we adapted a human neuronal progenitor cell line, hNP1, with ApoE genotype APOE3/3. Differentiating hNP1 cells were exposed for 16 days to HIV- or mock-infected supernatants and to added recombinant ApoE isoforms rApoE3 or rApoE4 to modulate the ApoE phenotype of the cells. Gene expression was investigated using microarray and functional genomics analyses. Added rApoE3 differentially affected 36 genes. Added rApoE4 differentially affected 85 genes; 41 of which were differentially expressed only in HIV or mock-supernatant treated cells, and 80% of which were downregulated. Genes differentially downregulated only by rApoE4 represented multiple neuronal functions related to neurogenesis. Approximately five times more genes were differentially enriched by rApoE4 versus rApoE3 in the Gene Ontology (GO) cellular process analysis, with 4 orders of magnitude greater significance. Half of the top 10 GO processes affected by rApoE4 treatment were neurogenesis-related. The largest differences in gene expression between the two isoforms were observed within the HIV-exposed cultures, suggesting that HIV exposure magnifies ApoE4's suppressive effect on neuronal gene expression. This study provides evidence for neuronal-specific responses to ApoE4 that could affect neurogenesis and neuronal survival
Keep Your Eye On the Ball (abstract)
No full textIn March 2014, an 82-year-old male developed left sided facial pain and numbness over his left temple and periorbital region. He was treated for a presumed dental infection with no response. MRI brain in July 2014; showed only microvascular white matter changes.1. Andreevscaia O et al. Diagnostic Challenge of Dermoplastic Melanoma. Rare Tumors, 8(1), 5713, 2016. 2. Barnett S et al. Perineural extension of cutaneous desmoplastic melanoma mimicking an intracranial malignant peripheral nerve sheath tumor. J Neurosurg, 115, 273-277, 2011. 3. Frydenlund N et al. Desmoplastic Melanoma, Neurotropism, and Neurotropin Receptors - What We Know and What We Do Not. Adv Anat Pathol, 22, 227-41, 2015. 4. Erkan et al. En Bloc Resection of Desmoplastic Neurotrophic Melanoma with Perineural Invasion of the Intracranial Trigeminal and Intraparotid Facial Nerve: Case Reports and Review of the Literature. J Neurol Surg Rep, 77, e8-e12, 2016.IC-D6biii-abducens-nerve-pals
Keep Your Eye On the Ball (video)
No full textIn March 2014, an 82-year-old male developed left sided facial pain and numbness over his left temple and periorbital region. He was treated for a presumed dental infection with no response. MRI brain in July 2014; showed only microvascular white matter changes.1. Andreevscaia O et al. Diagnostic Challenge of Dermoplastic Melanoma. Rare Tumors, 8(1), 5713, 2016. 2. Barnett S et al. Perineural extension of cutaneous desmoplastic melanoma mimicking an intracranial malignant peripheral nerve sheath tumor. J Neurosurg, 115, 273-277, 2011. 3. Frydenlund N et al. Desmoplastic Melanoma, Neurotropism, and Neurotropin Receptors - What We Know and What We Do Not. Adv Anat Pathol, 22, 227-41, 2015. 4. Erkan et al. En Bloc Resection of Desmoplastic Neurotrophic Melanoma with Perineural Invasion of the Intracranial Trigeminal and Intraparotid Facial Nerve: Case Reports and Review of the Literature. J Neurol Surg Rep, 77, e8-e12, 2016.IC-D6biii-abducens-nerve-pals
Myocardial preservation related to magnesium content of hyperkalemic cardioplegic solutions at 8 °C
No full textRelation of myocardial protection to cardioplegic solution pH: Modulation by calcium and magnesium
No full textQuantitative Analysis of Human Immunodeficiency Virus Type 1 Antibody Reactivity by Western Immunoblots: Evaluation of Relative Antibody Levels in Seropositive Individuals and Mothers
No full textA quantitative analysis of antibody responses to human immunodeficiency virus type 1 (HIV-1) proteins using Western immunoblots and 125I-labeled protein A is reproducible and can be validated. The antibody levels obtained by Western immunoblots were compared with stoichiometric p24 radioimmunoassay over a wide range of antibody (correlation coefficient, .94; P less than .001). Antibody levels to gp160 and gp120 were validated using purified antigens. Analysis of antibody levels from 31 seropositive individuals revealed a statistically significant correlation between antibody levels to p24 and the other viral proteins except gp120. Anti-gag p24 antibody was strongly correlated with antibodies to other env products, specifically gp41 and gp160. Using the validated assay, HIV-1-infected mothers of infants were found to have highly variable levels of antibody to all viral proteins. Mothers of infected infants did not differ significantly from mothers of uninfected infants in antibody pattern or levels to any viral protein including gp120
Treatment of Severe COVID-19 Infection With Remdesivir in Peritoneal Dialysis
No full textEnd-stage kidney disease (ESKD) has been shown to be correlated with an increased risk of COVID-19 infection and mortality. Remdesivir is an effective non-EUA U.S. Food and Drug Administration (FDA)-approved antiviral agent for the treatment of COVID-19 in hospitalized adult and pediatric patients, though a lack of data has prevented its use in patients with severe kidney disease including dialysis patients. Some observational studies report the use of remdesivir in hemodialysis patients, but there are no reports of patients treated with remdesivir on peritoneal dialysis. Dialysis modalities may affect drug pharmacokinetics, and safety and efficiency of remdesivir in peritoneal dialysis is unknown. We report the first case, to our knowledge, of using remdesivir in a patient treated with peritoneal dialysis with no significant adverse events. This case illustrates the potential for remdesivir to be considered in peritoneal dialysis patients with severe COVID infection. Proper risk analysis and careful monitoring should be done, given the unpredictable clearance of the drug
