1,720,990 research outputs found
Development of an optical RNA-based aptasensor for C-reactive protein
No full textdevelopment of aptasensing for C reactive protei
Improved analytical performances of aptamer-based sensing for thrombin detection Analytical Chemistry
No full textAptamer-based assays represent a modern and attractive approach in bioanalytical chemistry. The DNA thrombin aptamer has been extensively investigated, and the coupling of this aptamer to different transduction principles has demonstrated the wide applicability of aptamers as bioreceptors in bioanalytical assays. The goal of this work was to critically evaluate all the parameters that can influence the sensor performances by using the thrombin
aptamer immobilized onto piezoelectric quartz crystals.
The optimization of the immobilization and the binding protocol was of paramount importance, and improvements in analytical performances could be obtained by
optimizing simple steps in immobilization and assay conditions. Moreover, the work demonstrated the possibility of using aptamer-based sensors in complex matrixes,
opening the possibility of a real application to diagnostics or medical investigation
Aptamer-Based Detection of Plasma Proteins by an Electrochemical Assay Coupled to Magnetic Beads
No full textThe DNA thrombin aptamer has been extensively investigated,
and the coupling of this aptamer to different
transduction principles has demonstrated the wide applicability
of aptamers as bioreceptors in bioanalytical
assays. The goal of this work was to design an aptamerbased
sandwich assay with electrochemical detection for
thrombin analysis in complex matrixes, using a simple
target capturing step by aptamer-functionalized magnetic
beads. The conditions for the aptamer immobilization and
for the protein binding have been first optimized by
surface plasmon resonance, and then transferred to the
electrochemical-based assay performed onto screenprinted
electrodes. The assay was then applied to the
analysis of thrombin in buffer, spiked serum, and plasma
and high sensitivity and specificity were found. Moreover,
thrombin was generated in situ in plasma by the conversion
of its precursor prothrombin, and the formation of
thrombin was followed at different times. The concentrations
detected by the electrochemical assay were in
agreement with a simulation software that mimics the
formation of thrombin over time (thrombogram). The
proposed work demonstrates that the high specificity of
aptamers together with the use of magnetic beads are the
key features for aptamer-based analysis in complex matrixes,
opening the possibility of a real application to
diagnostics or medical investigation
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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