425 research outputs found
TELOMERASE REVERSE TRANSCRIPTASE PROMOTER MUTATIONS IN A COHORT OF ADULT GLIOMAS – CLINICOPATHOLOGICAL CORRELATES
No full textPurpose: This study characterizes diffuse gliomas (WHO grade II, III and IV) to determine the frequency of telomerase reverse transcriptase promoter (TERTp) mutations, association of TERTp mutations with other molecular alterations and to assess the role of TERTp mutation in overall survival and progression free survival in relation to histological and molecular glioma subtypes.
Methods: This study analyzed a cohort of 107 adult patients with diffuse gliomas, WHO grades II and III and glioblastoma, by immunohistochemistry for isocitrate dehydrogenase (IDH) and alpha-thalassemia/mental retardation, X-linked (ATRX) mutations, fluorescence in situ hybridization (FISH) for 1p/19q co-deletions and polymerase chain reaction (PCR) sequencing for TERTp mutation. Further, five glioma molecular sub-groups were derived using three molecular alterations and included the sub-groups with: i) IDH mutations only, ii) IDH and TERTp mutation only, iii) IDH and 1p/19q co-deletion only, iv) Triple negative and v) Triple positive.
Results: IDH mutations and 1p/19q co-deletions were individually and significantly associated with an improved progression free (p=0.001 and p=0.002 respectively) and overall survival (p=0.000 and p=0.005 respectively) in the present cohort of gliomas. TERTp mutations occurred frequently in anaplastic oligodendrogliomas (94%), oligodendrogliomas (87.5%) and glioblastomas (54%). Sub-division into molecular sub-groups showed that the triple positive tumors carried the best prognosis, followed by IDH only, triple negative and finally the TERTp mutation only tumors (p-value <0.000).
Conclusion: This indicates that sub-classification using these molecular markers separates tumors into prognostically relevant categories
Contribution of tin in electrochemical properties of zinc antimonate nanostructures: An electrode material for supercapacitors
No full textConcept of collective Nernstian-Capacitive mechanism in graphene nanosheets for electrochemical energy storage
No full textFabrication of bismuth ferrite based hybrid nanostructures: Insight into a catalytic and sensing properties for the detection of biomolecules
No full textNano-bioglass: A Versatile Antidote for Bone Tissue Engineering Problems
No full textAbstractBioactive-glass scaffolds are crucial in bone tissue engineering since they act as temporary templates for tissue regrowth, providing structural support to the cells. The enhancement of the angiogenic potential of implantable biomaterial scaffolds is receiving much attention in tissue engineering strategies. The angiogenic potential of most synthetic and natural materials used to fabricate tissue-engineered scaffolds is limited, insufficient, or even absent, numerous attempts have been made to enhance angiogenesis associated with tissue-engineered constructs, either by changing physicochemical properties or by supplementation with angiogenic factors. In order to improve the angiogenic effect of bioglass nano potash alum was incorporated to the Bioglass. Both Bioglass and alum doped Bioglass were synthesised by wet chemical method (sol-gel method) and its physiological properties were studied using TGA, XRD, FESEM, its cytocompatibility was studied by in vitro cell culture studies, and these bioglass are able to form a hydroxycarbonate apatite layer on their surface when in contact with an aqueous solution
Sunlight driven photocatalytic water splitting using nanostructured bismuth tungstate (Bi2WO6)
No full textDeriving magnetite nanostructures from natural resources and investigation of its erythrocyte compatibility
No full textIn vitro studies of graphene oxide reinforced hydroxyapatite nanobiocomposite on human erythrocytes
No full textAlteration of proteins and pigments influence the function of photosystem I under iron deficiency from Chlamydomonas reinhardtii.
No full textBACKGROUND: Iron is an essential micronutrient for all organisms because it is a component of enzyme cofactors that catalyze redox reactions in fundamental metabolic processes. Even though iron is abundant on earth, it is often present in the insoluble ferric [Fe (III)] state, leaving many surface environments Fe-limited. The haploid green alga Chlamydomonas reinhardtii is used as a model organism for studying eukaryotic photosynthesis. This study explores structural and functional changes in PSI-LHCI supercomplexes under Fe deficiency as the eukaryotic photosynthetic apparatus adapts to Fe deficiency. RESULTS: 77K emission spectra and sucrose density gradient data show that PSI and LHCI subunits are affected under iron deficiency conditions. The visible circular dichroism (CD) spectra associated with strongly-coupled chlorophyll dimers increases in intensity. The change in CD signals of pigments originates from the modification of interactions between pigment molecules. Evidence from sucrose gradients and non-denaturing (green) gels indicates that PSI-LHCI levels were reduced after cells were grown for 72 h in Fe-deficient medium. Ultrafast fluorescence spectroscopy suggests that red-shifted pigments in the PSI-LHCI antenna were lost during Fe stress. Further, denaturing gel electrophoresis and immunoblot analysis reveals that levels of the PSI subunits PsaC and PsaD decreased, while PsaE was completely absent after Fe stress. The light harvesting complexes were also susceptible to iron deficiency, with Lhca1 and Lhca9 showing the most dramatic decreases. These changes in the number and composition of PSI-LHCI supercomplexes may be caused by reactive oxygen species, which increase under Fe deficiency conditions. CONCLUSIONS: Fe deficiency induces rapid reduction of the levels of photosynthetic pigments due to a decrease in chlorophyll synthesis. Chlorophyll is important not only as a light-harvesting pigment, but also has a structural role, particularly in the pigment-rich LHCI subunits. The reduced level of chlorophyll molecules inhibits the formation of large PSI-LHCI supercomplexes, further decreasing the photosynthetic efficiency
A study on the behavior of pile supported footings on sand
No full textWhen the bearing capacity is not adequate to provide isolated footings, columns are grouped and supported combined footing. However, if the settlement increases beyond the permissible limits, piles are provided below the combined footing, applying the concept of piled raft. In this case the piles function as settlement reducers. Such systems can be categorized as a particular case of the combined piled raft system. In the present study a typical case of a piled raft with relatively smaller width, supported on two rows of piles on the edges was studied with small scale 1g model tests for understanding the basic behavior and a numerical study to obtain the details of raft settlement and the shaft stress distribution along the pile length.Full Tex
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