306 research outputs found
Overcoming Streptococcus agalactiae in vitro resistance to imidazoles
No full textThe increased diffusion of Streptococcus agalactiae in the urinary tract and vagina has affected the strain's resistance to antimicrobial agents, so we decided to study the possibility of overcoming its resistance to imidazoles. Our data suggest that overcoming S. agalactiae resistance to imidazoles in contact and growth culture tests depends partly on the electrical conductivity of the culture medium. Although imidazole contact activity and culture activity have different targets in cell structures, we have demonstrated that imidazole resistance in S. agalactiae tine cells in both types of tests can be affected by the same conditions regulating membrane permeability
miconazole and miconazole sulfosalicylate activity against Streptococcus agalactiae " Emergent microorganism"
No full textContact imidazole activity against resistant bacteria and fungi
No full textThe activities of miconazole and miconazole sulphosalicylate were evaluated by a contact test using strains of Enterococcus faecalis and Escherichia coli and Candida spp selected for their resistance. The results showed that killing of the organisms occured within a few minutes. Imidazole activity required a medium of low electrical conductivity and a pH of 5.6. Greater sensitivity could be obtained with pretreatment of the microbial suspensions with sodium dioctylsulphosuccinate. Microorganisms tested in culture media with low electrical conductivity and at pH 5.6 showed enhanced sensitivity to azoles with MIC values about 20-30 times lower than those obtained using control media. Practical implications of the use of topical drugs are discussed. (C) 2001 Elsevier Science B.V. and International Society of Chemotherapy. All rights reserved
Overcoming imidazole resistance in Escherichia coli
No full textWe have demonstrated that the high resistance to imidazoles in 60 Escherichia coli strains is overcome by lowering the minimum inhibitory concentration (MIC) in culture from >3200 to <0.39 mu g ml(-1) and, by determining, in contact tests, the disappearance of 10(4) CFU with 25 mu g ml(-1) of imidazoles after 3 min. Although the contact. activity was not the same as growth inhibition due to the different target of the drug in the cell structures, we annulled E. coli imidazole resistance in cultural and direct tests by means of the same non-antimicrobial conditions related to outer membrane functions
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