24,053 research outputs found
CHO-S master cell line generation.
No full text(A) The H11 locus was cleaved, using CRISPR/Cas9, to encourage integration of the landing pad donor. A successful knock-in at the H11 locus generates a master cell line that has two PhiC31 attP sites, and that co-expresses three proteins via a PGK promoter-driven transcript. (B) Genotyping of the 5’-arm and 3’-arm in the CHO-S master cell line. PCR was performed with genomic DNA and specific primer pairs. 1: CHO-S parental cell line with 5’-arm primers; 2: CHO-S master cell line ("4–6") with 5’-arm primers; 3: CHO-S parental cell line with 3’-arm primers; 4: CHO-S master cell line ("4–6") with 3’-arm primers.</p
Erratum: 3D bioprinted in vitro secondary hyperoxaluria model by mimicking intestinal-oxalatemalabsorption-related kidney stone disease (Applied Physics Reviews (2022) 9 (041408) DOI: 10.1063/5.0087345)
No full text© 2023 Author(s).This article was originally published online on 21 November 2022 with an incorrect affiliation identifier for author Dong-Woo Cho. It is correct as it appears above. All online versions of this article were corrected on 23 November 2022. AIP Publishing apologizes for this error.11Nsciescopu
Fujio Cho Legacy Lecture Notes
No full textThe Fujio Cho legacy lecture was created in 2013 as part of UK Institute of Research for Technology Development (IR4TD)’s True Lean Systems program to assist industrial clients to transform their organizations to a True Lean organization by effectively using principles and tools of Toyota Production System (TPS). During the years 1987-1994, Cho and Saito worked together to layout the foundation of the now well-established Toyota-University of Kentucky relationship on R&D, True Lean Systems, and production engineering, housed in IR4TD, the Toyota endowed Institute established in 2007 with the support from the Commonwealth of Kentucky under the research competitiveness trust fund. In 2019, this collaboration celebrated its 25th anniversary by recognizing True Lean Systems program serving over 30,000 people in eighteen different countries worldwide and 48 states nationally.
The idea of Fujio Cho Legacy Lecture Notes (FCLLN) was suggested to honor his wisdom and vision which are vital to maintain IR4TD/True Lean Systems program. FCLLN is written to provide philosophical and cultural background of TPS and Goroku, which are mentioned in the Fujio Cho legacy lecture. However, the human side of TPS, Hitozukuri, the manufacturing side of TPS, Monozukuri, and their interaction are not easily explained during an hour-long Cho lecture. Therefore, FCLLN plays into that role for attendees of IR4TD/True Lean Systems’ certification, and general audiences who are interested in TPS and are familiar with the concepts of Hitozukuri and Japanese Monozukuri culture. FCLLN covers a total of ten chapters: Chapter I. Introduction Chapter II. Toyota Production System and Goroku Chapter III. TPS and Wisdom Chapter IV. TPS and Empathetic Listening Chapter V. TPS as Unique Product of Japanese Culture Chapter VI. Deductive Science and Inductive TPS Chapter VII. Top-Down Power-Driven System vs. Bottom-up Kaizen System Chapter VIII. Cho Goroku on Service Chapter IX. Eastern Philosophy, Mother Teresa, and TPS Chapter X. Finally, the West and the East came together under the same principlehttps://uknowledge.uky.edu/ir4td_textbooks/1000/thumbnail.jp
Characterization of VH411-S-Tag peptide conjugate binding to the CHO-hLDLR-EGFP cell line.
No full text(A) Representative confocal photomicrographs of CHO-hLDLR-EGFP and CHO-hTfR-EGFP cells (green) incubated 1 hr at 37°C with 10 μM of VH411-S-Tag detected post-fixation with an anti-S-Tag and A647-conjugated secondary antibody (cyan) and 10 μg/mL of DiI-LDL. Cell nuclei are labeled with Hoechst#33258 (blue) at 0.5 μg/mL. Co-labeling appears in blue-green/orange in the merged pictures. Note that VH411-S-Tag co-localizes with hLDLR and is internalized by CHO-hLDLR-EGFP cells. (B) Representative confocal photomicrographs of CHO-hLDLR-EGFP cells (green) incubated 1 hr at 37°C with a macromolecular complex (see scheme Fig 3B) resulting from the co-incubation and interaction of 10 μM VH411-S-Tag peptide or VH411Sc-S-Tag peptide with the anti-S-Tag antibody (1/200) and the Alexafluor 647-conjugated secondary antibody (1/800) (cyan). Cells were concomitantly exposed to 10 μg/mL of DiI-LDL (red). Cell nuclei are labeled with Hoechst#33258 (blue). Co-labeling appears in blue-green/orange in the merged pictures.</p
Cho, Tse, & Chan - Normative Data for Chinese-English Translations
No full textCho, K.W., Tse, C-S., Chan, Y.L. (2019). Normative Data for English-Chinese Translations. Behavioral Research Methods. doi: 10.3758/s13428-019-01240-
Antigenicity and immunogenicity of SARS-CoV S protein receptor-binding domain stably expressed in CHO cells
No full textThe receptor-binding domain (RBD) of SARS coronavirus (SARS-CoV) spike (S) protein contains multiple conformation-dependent epitopes that induce neutralizing antibody responses. Here we used CHO-K1 cells to establish a cell line for stable expression of a 193-mer (residues 318-510) RBD (RBD193-CHO) and determined its antigenicity and immunogenicity. We found that RBD193-CHO reacted strongly with a panel of six monoclonal antibodies recognizing various conformational and linear epitopes in RBD, suggesting that this recombinant protein maintains intact conformation and good antigenicity. Immunization of mice with RBD193-CHO resulted in induction of high titers of RBD-specific neutralizing antibodies and potent IL-4-expressing T cell responses. RBD193-CHO induced immunity that protected a majority of the vaccinated mice from SARS-CoV challenge. These results suggest that the recombinant RBD produced in an established stable cell line maintains strong immunogenicity with high potential for use as an effective and economic subunit SARS vaccine. © 2009 Elsevier Inc. All rights reserved.link_to_OA_fulltex
Structural and functional definition of the specificity of a novel caspase-3 inhibitor, Ac-DNLD-CHO-3
No full text<p><b>Copyright information:</b></p><p>Taken from "Structural and functional definition of the specificity of a novel caspase-3 inhibitor, Ac-DNLD-CHO"</p><p>http://www.biomedcentral.com/1471-2210/7/8</p><p>BMC Pharmacology 2007;7():8-8.</p><p>Published online 27 Jun 2007</p><p>PMCID:PMC1931592.</p><p></p>en bonding and van der Waals interactions in the X-ray structures of caspases (codes 1PAU, 1F1J, 1F9E, and 1JXQ) and using examples as described [27], and then assigning the particular subsites (S, where x2 is the position in the Ssubsite). B, Schematic representation showing the locations of the subsites on the active site with Ac-DEVD-CHO. The underlined subsite has a conserved residue except the S(S/A) subsite
Acoustic Source Power Control and Global Noise Reduction by Arrangement of Absorptive Materials in Acoustically Small Enclosures
No full textThe possibility of global noise reduction by the sound power control is discussed. It is necessary to investigate the relation between the global sound energy in the field and the total sound power radiated by sources. In the previous work [1,2], the authors presented a useful design method to change boundary condition that reduces noise in acoustically small enclosures. Changing boundary condition is related to not only its geometrical shape but also acoustical treatment on walls; for example, attaching of impedance patches (ex: absorptive material). In many practical situations, we often meet a situation to change acoustical treatment on walls. A paper related to what we envisaged was presented at Inter-noise 2003 (S.-H. Cho, Y.-H. Kim, and K. Grosh, “The effect of impedance patch position on the sound field of an acoustically small cavity,” in Proc. Inter-noise 2003, N236). In this paper, the relation between acoustic source power control and global noise reduction are discussed. The possibility of total acoustic potential energy, which is global noise, reduction by acoustic source power control is examined for acoustically small cavity. Using acoustic energy balance equation, the relation between global noise control performance and absorptive material arrangement is deduced. Simulation is performed to investigate the theoretical possibility and the power control trend in terms of total acoustic potential energy reduction with respect to the frequency range of interest
Unimodality of Betti numbers for Hamiltonian circle actions with index-increasing moment Maps
No full textThe unimodality conjecture posed by Tolman in [L. Jeffrey, T. Holm, Y. Karshon, E. Lerman and E. Meinrenken, Moment maps in various geometries, http://www.birs.ca/workshops/2005/05w5072/report05w5072.pdf] states that if (M,ω) is a 2n-dimensional smooth compact symplectic manifold equipped with a Hamiltonian circle action with only isolated fixed points, then the sequence of Betti numbers {b0(M),b2(M),...,b2n(M)} is unimodal, i.e. bi(M) ≤ bi+2(M) for every i < n. Recently, the author and Kim [Y. Cho and M. Kim, Unimodality of the Betti numbers for Hamiltonian circle action with isolated fixed points, Math. Res. Lett. 21(4) (2014) 691-696] proved that the unimodality holds in eight-dimensional case by using equivariant cohomology theory. In this paper, we generalize the idea in [Y. Cho and M. Kim, Unimodality of the Betti numbers for Hamiltonian circle action with isolated fixed points, Math. Res. Lett. 21(4) (2014) 691-696] to an arbitrary dimensional case. We prove the conjecture in arbitrary dimension under the assumption that the moment map H : M → R is index-increasing, which means that ind(p) < ind(q) implies H(p) < H(q) for every pair of critical points p and q of H, where ind(p) is the Morse index of p with respect to H. © World Scientific Publishing Company1111sciescopu
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