9 research outputs found
Detection of C-type natriuretic peptide (CNP) transcript in the rat heart and immune organs
No full textPrevious studies suggested the expression of mRNA, coding for CNP, exclusively in the central nervous system. In the present study, using the polymerase chain reaction (PCR) technique instead of the less sensitive Northern blot hybridization, CNP-specific sequences have also been detected in rat atria and ventricles of the heart as well as in organs of the immune system (thymus, spleen and lymph nodes). Parallel PCR-assays documented ANP-mRNA in these tissues. To verify specificity of the PCR-products, Southern blots have been hybridized with a third internal oligonucleotide and amplification products have been sequenced. The relative level of CNP-mRNA in these tissues was estimated to be in the range of 1-9% of total brain CNP transcripts. The results suggest that the peptide may have a peripheral as well as a central site of action. In light of its pronounced effect on cell proliferation, particular interest should focus on a possible role of CNP in the immune system
The contralateral coordination of walking in the crayfish Astacus leptodactylus. II. Model calculations
No full textMüller U, Cruse H. The contralateral coordination of walking in the crayfish Astacus leptodactylus. II. Model calculations. Biol. Cybern. 1991;64(5):437-446
T lymphocytes from patients with primary biliary cirrhosis produce reduced amounts of lymphotoxin, tumor necrosis factor and interferon-gamma upon mitogen stimulation
No full textPrimary biliary cirrhosis (PBC) is considered an autoimmune disease characterized by destruction of small intrahepatic
bile ducts by lymphocytes. Altered functions of these lymphocytes might reflect an abnormal immune response leading
to tissue damage. We investigated lymphokine secretion by mitogen-stimulated T lymphocytes from the liver biopsies
of patients with PBC and for comparison also peripheral blood. In PBC, diminished synthesis of lymphotoxin (TNFP),
tumor necrosis factor (TNFa) and interferon-y (IFIVy) was found both in T-cell lines from liver tissue and in peripheral
blood. The reduction was most prominent for TNFP in early histological stages of PBC, and appeared to be a stable
phenomenon when T cells were tested after long-term tissue culture. Analysis of mRNA levels indicates a possible link
between reduced TNFP production and a defect in interleukin-2 transcription. The data suggest that diminished
lymphokine production in patients with PBC may play ;In important role in the immanopathogenesis of this disease
Localization of the genes for tumor necrosis factor and lymphotoxin between the HLA classI and III regions by field inversion gel electrophoresis
No full textTo clarify the position of the TNFA and TNFB genes on the HLA map, we have assigned TNFA to large DNA restriction fragments separated by field inversion gel electrophoresis, which hybridize with either class III- or class I-specific probes as well. These results prove that the TNFA locus is localized between the HLA class III region and the HLA-B locus
High mass photon pairs in lepton+ lepton-gamma gamma events at LEP
No full textHigh mass photon pairs in lepton+ lepton-gamma gamma events at LEP Adriani, O.; Aguilar-Benitez, M.; Ahlen, S.P.; Alcaraz, J.; Aloisio, A.; Alverson, G.; Alviggi, M.G.; Ambrosi, G.; Linde, F.L. Published in: Physics Letters B DOI: 10.1016/0370-2693(92)91576-U Link to publication Citation for published version (APA): Adriani, O., Aguilar-Benitez, M., Ahlen, S. P., Alcaraz, J., Aloisio, A., Alverson, G., ... Linde, F. L. (1992). High mass photon pairs in lepton+ lepton-gamma gamma events at LEP. Physics Letters B, 295,[337][338][339][340][341][342][343][344][345][346] https://doi.org/10.1016/0370-2693(92)91576-U General rights It is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulations If you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: https://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. Download date: 28 Jun 2019 Physics Letters B 295 (1992) From the analysis of the reactions e + e-~ g + g-(n?) (g = e, #, ~) we observe four events, one e+e -~'7 and three #+ ~-??, with the invariant mass of the photon pairs close to 60 GeV. These events were selected from a data sample collected in the L3 detector corresponding to 950000 produced Z°'s. More data are necessary to ascertain the origin of these events
Genetic and serological heterogeneity of the supertypic HLA-B locus specificities Bw4 and Bw6
No full textGene cloning and sequencing of the HLA-B
locus split antigens B38 (B16.1) and B39 (B16.2) allowed
localization of their subtypic as well as their public
specificities HLA-Bw4 or -Bw6 to the c~-helical region of
the c~ 1 domain flanked by the amino acid positions 74-83.
Comparison of their amino acid sequences with those of
other HLA-B-locus alleles established HLA-Bw6 to be
distinguished by Ser at residue 77 and Asn at residue 80.
In contrast, HLA-Bw4 is characterized by at least seven
different patterns of amino acid exchanges at positions 77
and 80-83. Reactivity patterns of Bw4- or Bw6-specific
monoclonal antibodies reveal two alloantigenic epitopes
contributing to the HLA-Bw4 or -Bw6 specificity residing
next to the region of highest diversity of the cr domain
The influences of crustal extension, salt tectonics and gravity-driven deformation on the structural evolution of the Halten Terrace, offshore mid-Norway: new sights from 3D seismic data and fault analysis
No full textNormal fault zones play a fundamental role in the development of sedimentary basins and in the migration and trapping of hydrocarbons. The idealised geometry of an isolated post-sedimentary normal fault (Barnett, 1987, Walsh & Watterson, 1989) existing conceptual models that describe the process of fault growth and linkage in brittle systems (Childs et al, 1995; Cartwright et al, 1996; Childs et al, 1995, 1996b; Huggins et al, 1995), where fault planes composed of many overstepping segments are linked by areas of complex deformation called relay ramps, are generally accepted. Relay zones can trap significant volumes of hydrocarbon or act as leakage points, thus understanding the style of fault linkage, which strongly influences the location of hydrocarbon tops and reservoir compartmentalisation, is vital for any petroleum system.EThOS - Electronic Theses Online ServiceGBUnited Kingdo
Early steps in mitochondrial protein import
No full textThe process of insertion of precursor proteins into mitochondrial membranes was investigated using a hybrid protein (pSc1-c) that contains dual targeting information and, at the same time, membrane insertion activity. pSc1-c is composed of the matrix-targeting domain of the cytochrome c1 presequence joined to the amino terminus of apocytochrome c. It can be selectively imported along either a cytochrome c1 route into the mitochondrial matrix or via the cytochrome c route into the intermembrane space. In contrast to cytochrome c1, pSc1-c does not require the receptor system/GIP for entry into the matrix. The apocytochrome c in the pSc1-c fusion protein appears to exert its membrane insertion activity in such a manner that the matrix-targeting sequence gains direct access to the membrane potential-dependent step. These results attribute an essential function to the receptor system in facilitating the initial insertion of precursors into the mitochondrial membranes
